Vitamin C levels in brain/liver of trout fed lapsi fruit extract
Journal name: World Journal of Pharmaceutical Research
Original article title: Biochemical estimations of vitamin c in brain and liver of rainbow trout oncorhynchus mykiss fed ethanol extract of lapsi fruit (eelf) choerospondias axillaris (roxb.) in raceways fisheries at nuwakot, nepal
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Shubha Ratna Shakya and Shyam Narayan Labh
World Journal of Pharmaceutical Research:
(An ISO 9001:2015 Certified International Journal)
Full text available for: Biochemical estimations of vitamin c in brain and liver of rainbow trout oncorhynchus mykiss fed ethanol extract of lapsi fruit (eelf) choerospondias axillaris (roxb.) in raceways fisheries at nuwakot, nepal
Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research
Doi: 10.20959/wjpr201818-14809
Copyright (license): WJPR: All rights reserved
Summary of article contents:
1) Introduction
Vitamin C (ascorbic acid) is a crucial nutrient for freshwater fish, including rainbow trout (Oncorhynchus mykiss). Unlike terrestrial animals, fish do not possess the enzyme L-gulonolactone oxidase (GLO) necessary for synthesizing vitamin C, making it vital to include this nutrient in their diets. Previous research indicates that deficiencies in vitamin C can lead to poor growth rates and increased susceptibility to diseases in fish. Given the oxidative sensitivity of vitamin C during processing, the fruit of lapsi (Choerospondias axillaris), which is rich in vitamin C, was used in this study to evaluate its efficacy in improving the health and growth of rainbow trout.
2) The Role of Vitamin C in Fish Nutrition
Vitamin C not only promotes growth and robust immune function but also acts as a water-soluble antioxidant that plays a role in collagen formation, reproductive processes, and overall disease resistance in fish. The study highlights that fish fed diets deficient in vitamin C exhibit several signs of deficiency, including growth retardation and increased mortality. Most commercial fish feeds are low in vitamin C, necessitating its supplementation. Lapsi fruit, known for its high vitamin C content, serves as a natural alternative for enhancing fish diets, thus supporting their immune systems and overall health.
3) Experimental Design and Findings
The experiments were conducted over a 90-day period at Sosoda Trout Farm in Nuwakot, Nepal, where 450 rainbow trout were divided into six groups. Each group received a semi-purified diet supplemented with different concentrations of ethanol extract from lapsi fruit, ranging from 0 to 1600 mg/kg of diet. Results showed that diets with lapsi extracts led to increased weight gain and higher concentrations of vitamin C in both the brain and liver tissues compared to the control group without the extract. Especially significant was the enhanced vitamin C concentration observed in the group fed with the optimal dose of lapsi extract.
4) Impact on Immune Response and Health
The findings suggest that dietary supplementation with lapsi not only improves vitamin C levels but also positively impacts the immune response of the trout. Increased dietary vitamin C is associated with enhanced non-specific immune parameters, indicating potential for improved disease resistance. The study concludes that high vitamin C levels, particularly from natural sources like lapsi fruit, may prove beneficial in enhancing the fish's ability to resist infections caused by pathogens, thereby reducing reliance on antibiotics for disease management in aquaculture settings.
5) Conclusion
The study establishes the essential nature of vitamin C for the growth and wellbeing of rainbow trout and suggests that incorporating lapsi fruit extracts into their diet can effectively meet their nutritional needs. The results indicate that such dietary interventions can facilitate better growth rates and bolster the immune system, allowing trout to be more resilient against diseases. This research opens potential avenues for developing natural dietary supplements in aquaculture, promoting healthier fish farming practices while minimizing antibiotic use.
FAQ section (important questions/answers):
What is the purpose of this study on rainbow trout?
The study aimed to evaluate the effects of dietary Vitamin C, sourced from lapsi fruit extract, on the growth and vitamin C concentration in the brain and liver of rainbow trout.
Why is Vitamin C important for rainbow trout?
Rainbow trout lack the enzyme L-gulonolactone oxidase necessary for synthesizing Vitamin C. Therefore, it is crucial to supplement their diet with Vitamin C to support growth, immune response, and overall health.
What were the dietary treatments applied in the study?
Trout were divided into six groups, receiving diets supplemented with varying concentrations of ethanol extract of lapsi fruits (0, 100, 200, 400, 800, and 1600 mg/kg) over 12 weeks.
How did lapsi fruit extract affect the trout's vitamin C levels?
Fish fed diets with lapsi fruit extract showed significantly higher Vitamin C concentrations in both the brain and liver compared to control groups, with the T4 diet showing optimal results.
What were the key findings related to fish growth and immunity?
Diets enriched with lapsi extract improved growth rates and enhanced non-specific immune responses in trout, indicating that dietary Vitamin C can boost disease resistance in fish.
Who supported the research conducted on vitamin C in trout?
The research was supported financially and logistically by the Head of Central Department of Zoology, Prof. Dr. Ranjana Gupta, and the Vice Chancellor of NAST, Prof. Dr. Jivaraj Pokharel.
Glossary definitions and references:
Scientific and Ayurvedic Glossary list for “Vitamin C levels in brain/liver of trout fed lapsi fruit extract”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.
1) Diet:
The term 'Diet' refers to the specific nutritional plan created by incorporating various ingredients, in this case, experimental diets for the rainbow trout. Various formulations, including the ethanol extract of lapsi fruits, were tested to assess how different nutrition levels influence fish growth and health.
2) Fish:
'Fish' specifically relates to the subject of the study, rainbow trout (Oncorhynchus mykiss), an important fresh-water species known for its cold-water habitat. The research emphasizes the nutritional needs of fish, particularly the critical role of Vitamin C in their diets for optimal growth and health.
3) Water:
'Water' is essential for the aquaculture environment of rainbow trout. The study highlights the importance of clean, cold water conditions for proper fish culture. It refers to the natural habitat and the controlled environments maintained in raceway ponds necessary for the survival and growth of trout.
4) Lapsi:
'Lapsi' refers to the fruit of Choerospondias axillaris, known for its high Vitamin C content. The study investigates the potential of lapsi as a dietary supplement, emphasizing its use in enhancing the vitamin intake of rainbow trout and supporting their immune function and growth.
5) Shubha (Subha):
'Shubha' is the first author of the research paper, indicating her primary role in conducting the study. Her insights and findings contribute significantly to the scientific understanding of vitamin C supplementation in fish diets and hold importance in the broader context of aquaculture research.
6) Activity:
'Activity' refers to the physiological responses or functions observed in the trout, particularly with respect to the effects of dietary Vitamin C on immune responses. The study assessed various activities linked to growth and health outcomes resulting from different dietary treatments with lapsi.
7) Disease:
'Disease' pertains to health challenges that fish may face due to nutritional deficiencies, specifically Vitamin C deficiency. The study discusses how appropriate dietary supplementation can enhance disease resistance in trout, suggesting a critical relationship between nutrition and the prevention of illnesses in aquaculture.
8) Cage:
'Cage' denotes the method of housing trout in controlled environments for the study. The research utilized nylon cages placed in raceway ponds to regulate feeding trials and monitor growth, highlighting an important aspect of fish farming techniques and experimental aquaculture.
9) Zoology:
'Zoology' is the field of science that encompasses the study of animals, including the biology and behavior of fish like the rainbow trout. The research falls under zoological studies, particularly focusing on aquatic species, their nutritional needs, and their physiological responses to dietary supplements.
10) Rich (Rch):
'Rich' describes the high concentration of Vitamin C in lapsi fruit. The research emphasizes the nutritional richness of lapsi as a natural dietary source, showing its advantage over synthetic vitamin supplements and underscoring the importance of incorporating natural sources into fish diets for better health outcomes.
11) Village:
'Village' refers to Ranipauwa, a region noted for its trout farming activities in Nepal. This setting is crucial for the study, as it provides a natural context for understanding aquaculture practices and the socio-economic impact of fish farming on local communities.
12) Animal:
'Animal' in this context refers to fish, specifically the rainbow trout studied in the research. It emphasizes the biological and ecological aspects of fish as living organisms, alongside their nutritional requirements and how appropriate care enhances their health and growth in aquaculture settings.
13) Powder:
'Powder' typically relates to the feed ingredients used in the diets formulated for the trout, such as dry fish powder. The research explores how these components alongside lapsi extract contribute to the overall nutritional profile and effectiveness of the feed in promoting growth.
14) Campu:
'Campu' likely refers to the campus environment of the Central Department of Zoology at Tribhuvan University, where the research is based. This provides a scholarly context for the study, linking academic resources and expertise to practical applications in fish nutrition and aquaculture.
15) India:
'India' is relevant as it mentions the origin of some feed materials used in the diets, which were procured from the local market. This indicates the influence of international suppliers on local fish farming practices and emphasizes the role of regional trade in aquaculture.
16) Soya:
'Soya' is included as one of the feed components, a common protein source in fish diets. It highlights the use of plant-based ingredients in aquaculture feeds, which is essential for providing balanced nutrition and supporting optimal growth and health of fish like rainbow trout.
17) Kathmandu:
'Kathmandu' denotes the location where certain feed ingredients were sourced for the experiment. The capital city represents a central hub for trade in Nepal, facilitating access to various nutritional resources important for aquaculture and related research initiatives.
18) Species:
'Species' particularly refers to the rainbow trout, which is the focal point of this study. Understanding the species-specific nutritional requirements is critical for designing effective feeding protocols that maximize growth and health in aquaculture systems.
19) Mineral:
'Mineral' refers to the added vitamins and mineral premix in the formulated diets, which ensure that the trout receive a balanced nutritional intake. The presence of essential minerals in the diet is vital for normal physiological functions and enhancing the overall health of fish.
20) Shakya (Sakya):
'Shakya' is the name of the corresponding author, indicating a key figure in the research study. This highlights individual contributions to the scientific discourse surrounding aquaculture nutrition and furthers collaboration among experts in the field.
21) Kakani:
'Kakani' is the locality related to the research site, specifically the Sosod Trout Farm where the experiments were conducted. This geographic context emphasizes the relevance of localized study in understanding the practical applications of aquaculture and fish nutrition.
22) Ratna:
'Ratna' is part of the author's name, tying to Shubha Ratna Shakya, who plays a pivotal role in this research. This denotes the importance of specific contributors to academic studies and illustrates individual expertise within collaborative efforts in the scientific community.
23) Table:
'Table' refers to the data presentation structure used in the research to summarize the composition of experimental diets, providing clear statistics on feed ingredients. It plays an essential role in facilitating understanding of dietary formulation and nutritional content for trout.
24) Study (Studying):
'Study' encapsulates the entire research project focusing on vitamin C levels in rainbow trout and their dietary influences. This highlights the methodological approach taken to investigate the nutritional requirements and health outcomes while contributing to expanding knowledge in aquaculture.
25) Rice (Rce):
'Rice' is one of the dietary components used in the formulated feeds for the trout. As a carbohydrate source, its inclusion contributes to the overall energy content of the diet, thus playing an essential role in supporting growth and metabolic needs of the fish.
26) Sign:
'Sign' pertains to the indicators of health and nutritional status observed in the fish throughout the study. Recognizing physiological signs associated with deficiencies aids in developing better dietary strategies to enhance growth and health among trout in aquaculture contexts.
27) Pur:
'Poor' describes the adverse outcomes, such as reduced growth rates, seen in trout not receiving adequate vitamin C. This term underscores the importance of sufficient nutrient intake in fish diets to prevent deficiencies that hamper growth and overall health.
28) Phytochemical:
'Phytochemical' refers to the beneficial compounds found in lapsi fruit that can have positive health effects. The study discusses the potential use of natural sources rich in phytochemicals as alternatives to synthetic additives in fish feeds to enhance health and growth performance.
29) Discussion:
'Discussion' reflects the analytical section of the research that interprets findings and their implications. It emphasizes the importance of results in relation to previous studies and highlights how the incorporation of lapsi can benefit aquaculture practices through improved fish health.
30) Antibiotic (Antibacterial):
'Antibiotic' discusses the potential use of natural products like lapsi as alternatives to conventional antimicrobial treatments in aquaculture. The study suggests that focusing on natural immune-boosters can reduce reliance on antibiotics while maintaining fish health and preventing disease outbreaks.
31) Ranjana (Ramjana):
'Ranjana' refers to Prof. Dr. Ranjana Gupta, a key figure acknowledged in the study for providing support and expertise. This highlights the collaborative nature of research and the importance of mentorship in guiding scientific inquiry in aquaculture and related disciplines.
32) Quality:
'Quality' refers to the standard of the water, feeds, and overall environment in which fish are raised. The study emphasizes maintaining high quality in all aspects of trout culture to support optimal growth, health, and resistance to diseases within aquaculture systems.
33) Raigad:
'Raigad' indicates the origin of some feed ingredients utilized in the diets. It connects the research to broader geographical trade networks, demonstrating the significance of regional resources in formulating effective nutritional plans for aquaculture and the implications for sustainable fishing practices.
34) Family:
'Family' in this context refers to the classification of rainbow trout under the Salmonidae family. Understanding the biological characteristics of this family provides insights into its nutritional needs and management practices for aquaculture, ensuring the health and productivity of these fish.
35) Valley:
'Valley' relates to the Kathmandu Valley region, where key components for the study, like feed ingredients, were procured. This emphasizes the geographical and ecological context that supports aquaculture initiatives and the importance of local resources in fish farming.
36) Mitra:
'Mitra' refers to a researcher acknowledged in the study, suggesting that collaborative work builds on existing knowledge. The mention of previous studies conducted by Mitra adds credibility to the current research, situating it within a broader academic discourse on fish nutrition.
37) Gupta:
'Gupta' refers to the author, Prof. Dr. Ranjana Gupta, who played a critical role in the study. Her contributions underline the importance of academic leadership and support in research endeavors, particularly those focused on nutrition and aquaculture.
38) Field:
'Field' encompasses the practical application and implications of the research within aquaculture. It signifies the relevance of laboratory findings to real-world fish farming practices, impacting growth strategies and health management for trout and similar species.
39) Ruci:
'Ruchi' refers to Ruchi Soya Industries, the supplier noted for its contribution to the feed components. This connects the research to industry practices and highlights the significance of commercial partnerships in supporting aquaculture initiatives and enhancing dietary formulation for fish.
Other Science Concepts:
Discover the significance of concepts within the article: ‘Vitamin C levels in brain/liver of trout fed lapsi fruit extract’. Further sources in the context of Science might help you critically compare this page with similair documents:
Growth rate, Rainbow trout, Non-specific immunity, Antioxidant vitamin, Recirculation system.