Analytical standardization of shatpala gandhaka churna

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Journal name: World Journal of Pharmaceutical Research
Original article title: Analytical standardization of shatpala gandhaka churna
The WJPR includes peer-reviewed publications such as scientific research papers, reports, review articles, company news, thesis reports and case studies in areas of Biology, Pharmaceutical industries and Chemical technology while incorporating ancient fields of knowledge such combining Ayurveda with scientific data.
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Author:

Hema T., Venkata Subbaiah K. and Sridurga Ch.


World Journal of Pharmaceutical Research:

(An ISO 9001:2015 Certified International Journal)

Full text available for: Analytical standardization of shatpala gandhaka churna

Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research

Doi: 10.20959/wjpr201819-13728

Copyright (license): WJPR: All rights reserved


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Summary of article contents:

Introduction

The study focuses on the analytical standardization of Shatpala Gandhaka Churna, an important Ayurvedic formulation primarily utilized in Rasa Shastra, a branch of Ayurveda dedicated to unique pharmaceutics. The formulation comprises various potent ingredients, including Shuddha Gandhaka, Shuddha Bhallataka, and several herbal components. As modern techniques become essential for the validation of traditional remedies, the analytical evaluation presented in this study aims to assess the formulation's safety, toxicity, and chemical composition through various advanced analytical methods.

Analysis of Particle Size and Zeta Potential

One of the significant findings of the study is concerning the particle size and zeta potential of Shatpala Gandhaka Churna. The mean particle size was determined to be 10.9 nm, indicating that the formulation is in the nanoparticle range. This nanoparticle dimension suggests that the drug can be rapidly absorbed and effectively dispersed within the body, enhancing therapeutic efficacy. Furthermore, the zeta potential of -40.9 mV indicates a high level of colloidal stability, which allows for better dispersion of particles in suspension. This stability is crucial for maintaining the efficacy of the formulation in therapeutic applications.

X-Ray Diffraction (XRD) Analysis

Another important aspect of the analysis is the X-Ray Diffraction (XRD), which revealed significant peaks for Sulphur (S₈) and other compounds such as Sodium Magnesium Sulfate (Vanthoffite). The presence of Sulphur highlighted its high crystalline nature and abundance within the formulation. The XRD findings provide a fingerprint characterization of the crystalline materials, enabling the identification of the formulation's compositions through comparisons with established crystallographic data. This method serves as a reliable tool for confirming the structural integrity and quality of Ayurvedic preparations.

Fourier Transform Infrared Spectroscopy (FTIR)

The study also employed Fourier Transform Infrared Spectroscopy (FTIR) to identify the presence of functional groups in Shatpala Gandhaka Churna. The analysis detected ten significant peaks, indicating various functional groups such as alkanes and alcohols. This method underscores the complex nature of the formulation and affirms that it does not contain any overly complex structures. By providing insights into the molecular interactions and constituents of the churna, FTIR contributes to a comprehensive understanding of the formulation's therapeutic potential.

Conclusion

In conclusion, the analytical study of Shatpala Gandhaka Churna demonstrates the formulation's efficacy and safety as a herbo-mineral compound, with Sulphur emerging as the principal component. The nanoscale size of the particles suggests efficient absorption and therapeutic action, while the absence of toxic elements supports the formulation's safety for consumption. This research not only validates the ancient Ayurveda practices but also bridges traditional knowledge with modern scientific methodologies, enhancing the credibility and acceptance of Ayurvedic formulations in contemporary therapeutic contexts.

FAQ section (important questions/answers):

What is Shatpala Gandhaka Churna and its significance?

Shatpala Gandhaka Churna is a herbo-mineral compound significant in Ayurveda, primarily used for developing potent formulations in Rasa Shastra, which focuses on the pharmaceutical aspects of Ayurvedic medicine.

What analytical techniques were used for the study?

Various modern analytical techniques were employed, including X-ray diffraction (XRD), scanning electron microscopy (SEM), particle size analysis (PSA), zeta potential (ZP), UV-spectroscopy, FT-IR, and ICP-OES for comprehensive standardization and analysis.

What were the major findings from the XRD analysis?

XRD analysis revealed major peaks of Sulphur (S8) and confirmed the presence of Sodium Magnesium Sulfate (Vanthoffite), indicating the crystalline nature and constituent composition of Shatpala Gandhaka Churna.

What was the particle size determined for Shatpala Gandhaka Churna?

The mean particle size of Shatpala Gandhaka Churna was found to be 10.9 nm, suggesting its potential for quick absorption and effective therapeutic action due to its nano size.

Were any toxic elements detected in the analysis?

No toxic elements such as arsenic, cadmium, or lead were detected in Shatpala Gandhaka Churna, indicating the safety of the formulation for therapeutic usage.

What is the significance of zeta potential in this study?

The zeta potential of -40.9 mV indicates high colloidal stability of Shatpala Gandhaka Churna, which is important for ensuring consistent dispersion and bioavailability of the formulation.

Glossary definitions and references:

Scientific and Ayurvedic Glossary list for “Analytical standardization of shatpala gandhaka churna”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.

1) Gandhaka (Gamdhaka):
Gandhaka refers to sulfur, which is a critical component of Shatpala Gandhaka Churna, specifically processed in traditional Ayurveda. Known for its medicinal properties, Gandhaka plays a vital role in Rasa Shastra, where herbs and minerals are combined to enhance therapeutic efficacy in formulations used for various ailments.

2) Hema (Heman):
Hema is a name associated with Dr. Hema T., the corresponding author of the research article. As a PG Scholar in Ayurveda, she has contributed significantly to the analytical standardization of Ayurvedic formulations, bridging ancient knowledge with modern scientific methods to assess safety and efficacy in drug preparation.

3) Study (Studying):
The study discusses the analytical standardization of Shatpala Gandhaka Churna, employing modern scientific parameters to validate ancient Ayurvedic practices. This research aims to provide insights into the formulation's composition, safety, and therapeutic potential, aligning traditional approaches with contemporary scientific scrutiny, which is essential for herbal medicine validation.

4) Bhallataka:
Bhallataka, also known as Semecarpus anacardium, is an essential ingredient in Shatpala Gandhaka Churna. In Rasa Shastra, it undergoes specific purification (Shodhana) processes to enhance its therapeutic properties. Its inclusion signifies the importance of natural substances, bolstering the formulation's efficacy and safety against certain ailments.

5) Drug:
In the context of the research, 'drug' refers to the formulated Shatpala Gandhaka Churna. The formulation includes various herbal and mineral components that undergo extensive analytical studies to determine their safety, efficacy, and quality. It emphasizes the significance of understanding the pharmacological aspects of traditional Ayurvedic medicines.

6) Tirupati (Thirupati, Tiruppati):
Tirupati is a prominent city in Andhra Pradesh, India, recognized for its cultural and spiritual significance. The study is conducted at S. V. University and S. V. Ayurvedic College in Tirupati, where traditional Ayurvedic practices are upheld. This location reinforces the connection between ancient knowledge and modern scientific investigation.

7) Amalaki:
Amalaki, or Emblica officinalis, is an important medicinal herb used in the preparation of Shatpala Gandhaka Churna. It possesses numerous health benefits, including antioxidant properties, and is integral to several Ayurvedic formulations. Its inclusion in the study highlights the use of polyherbal compositions in traditional medicine.

8) Surface:
Surface refers to the physical characteristics observed through techniques such as Scanning Electron Microscopy (SEM) that provide insight into the morphology of Shatpala Gandhaka Churna. Understanding surface features is crucial in the study of drug delivery systems and the effectiveness of herbal formulations in therapeutic applications.

9) Shunthi (Sunthi, Sumthi, Shumthi):
Sunthi, or Zingiber officinale (ginger), is included in Shatpala Gandhaka Churna for its gastrointestinal benefits and anti-inflammatory effects. The presence of Sunthi reflects the importance of synergistic herbal combinations in Ayurveda, which enhance the overall therapeutic properties of the formulation for managing various health conditions.

10) Shodhana (Sodhana):
Shodhana refers to the purification processes employed in Ayurveda to detoxify and enhance the properties of raw materials, particularly in metal and mineral drugs. The study utilizes Shodhana of ingredients like Gandhaka and Bhallataka, demonstrating the traditional practices vital for ensuring the safety of formulations used in medicinal treatments.

11) Powder:
Powder indicates the physical form of the ingredients used in the creation of Shatpala Gandhaka Churna. The grinding and processing into fine powder increase the bioavailability and absorption of the herbal components, thereby improving their efficacy in therapeutic applications as part of Ayurvedic medicine.

12) Rasa (Rasha):
Rasa refers to the taste and also a branch of Ayurveda focused on the alchemical preparation and spiritual transformation of metals and minerals called Rasa Shastra. In this study, Rasa emphasizes the specialized techniques employed in preparing Shatpala Gandhaka Churna, contributing to its effectiveness in treating ailments.

13) Haritaki:
Haritaki, or Terminalia chebula, is a prominent Ayurvedic herb known for its detoxifying abilities. Its inclusion in Shatpala Gandhaka Churna enhances digestive properties and balances doshas. The study showcases how traditional herbal combinations are used to formulate drugs with multifaceted health benefits based on ancient wisdom.

14) Pippali (Pippalin):
Pippali, or Piper longum, is a significant herb in Ayurveda known for its effectiveness as a digestive and respiratory aid. Its presence in Shatpala Gandhaka Churna ensures the formulation's therapeutic range, reflecting the principles of Ayurveda where specific herbs are selected for their synergistic effects in a composite formulation.

15) Shuddha (Suddha):
Shuddha means 'pure' in Sanskrit, signifying the purified state of the ingredients before their use in formulation. The term underscores the emphasis on quality and safety in the production of Shatpala Gandhaka Churna, ensuring that the substances are free of contaminants and suitable for medicinal use.

16) Table:
The term 'Table' in the research context refers to organized data or results presented succinctly for easy comprehension. It may include numerical data on XRD results, chemical compositions, or ICPOES analyses, allowing researchers and practitioners to quickly assess the findings and implications related to Shatpala Gandhaka Churna.

17) Vibhitaki:
Vibhitaki, or Terminalia bellirica, is another herbal ingredient used in the formulation of Shatpala Gandhaka Churna. Rich in tannins and known for its astringent properties, it enhances the formulation's health benefits. Its role showcases the traditional Ayurvedic practice of using multiple herbal sources to create synergistic effects.

18) Trikatu (Tri-katu):
Trikatu is a traditional Ayurvedic formulation composed of black pepper, long pepper, and ginger. Its inclusion in Shatpala Gandhaka Churna boosts metabolic functions and enhances absorption. The study highlights the role of such combinations in ensuring comprehensive benefits and optimal effectiveness in Ayurvedic treatments is achieved.

19) Mineral:
Mineral refers to inorganic elements critical for various biochemical functions in the body found in Shatpala Gandhaka Churna. The study investigates these mineral constituents through ICP-OES to assess their levels, ensuring that they contribute positively to the therapeutic profile of the formulation while maintaining safety.

20) Science (Scientific):
Scientific pertains to the approaches and methodologies employed in the analysis of Shatpala Gandhaka Churna. This study exemplifies the application of rigorous scientific principles to traditional Ayurvedic preparations, ensuring that ancient practices align with modern standards of validation, assessment, and quality control for medicinal compounds.

21) Rasashastra (Rasa-shastra, Rasasastra):
Rasa-shastra is an Ayurvedic discipline dedicated to the study and processing of metals and minerals for therapeutic use. The formulation Shatpala Gandhaka Churna emerges from this scientific tradition, employing meticulous pharmaceutical methods alongside modern analytical techniques to ensure safety, efficacy, and quality in herbal medicine.

22) Trijataka (Tri-jataka):
Trijataka, or Asteracantha longifolia, is a key ingredient in the herbal formulation. It serves various medicinal purposes, including enhancement of digestion and excretion. Its inclusion in Shatpala Gandhaka Churna showcases the use of multi-modal herbal systems in Ayurveda to create compounds aiming for systemic health improvement.

23) Transformation (Transform, Transforming):
Transform highlights the process of converting raw materials into effective medicinal formulations through the integration of traditional Ayurvedic techniques with modern scientific validation. This dual approach not only preserves ancient wisdom but also advances it by ensuring formulations like Shatpala Gandhaka Churna are safe and effective for contemporary use.

24) Ayurveda (Ayus-veda):
Ayurveda is an ancient holistic healing system originating from India, emphasizing balance within the body, mind, and spirit. The study focuses on Shatpala Gandhaka Churna, showcasing its role in Ayurvedic medicine through rigorous analysis, highlighting Ayurveda's integration with modern scientific practices for safety and efficacy in herbal formulations.

25) Triphala (Tri-phala):
Triphala is a revered combination of three fruits used in Ayurveda known for its detoxifying and rejuvenating properties. Its inclusion in Shatpala Gandhaka Churna signifies the importance of comprehensive herbal systems that support health and healing, encapsulating the Ayurvedic belief in holistic well-being through synergistic formulations.

26) Shastra (Sastra):
Shastra refers to the comprehensive body of traditional knowledge and scriptures pertaining to various disciplines in Ayurveda. In this context, Rasa Shastra, a subset of Ayurvedic science based on wisdom and practices is foundational for the formulation of Shatpala Gandhaka Churna, ensuring its historical and therapeutic significance.

27) Vidanga (Vidamga):
Vidanga, or Embelia ribes, is an important herb that is part of the Shatpala Gandhaka Churna. It plays a crucial role in digestive health, acting as a carminative. The utilization of Vidanga emphasizes Ayurveda's focus on using natural substances for enhancing health and treating various ailments holistically.

28) Nature:
Nature signifies the foundational source of herbal and mineral components used in Shatpala Gandhaka Churna. The study underscores the essence of harnessing natural resources for formulating drugs that embrace the therapeutic potential of plants and minerals, advocating for sustainable practices in medicine and health care.

29) Medium:
Medium may refer to the vehicles or solvents used in the preparation and analysis of Shatpala Gandhaka Churna. The choice of medium affects solubility, absorption, and overall efficacy in therapeutic applications, stressing the importance of proper formulation in maximizing the benefits of Ayurvedic medicines.

30) India:
India is the geographical origin of Ayurveda, a system deeply embedded in its cultural practices and medicinal traditions. The research conducted in Indian institutions highlights the country's commitment to preserving and validating its ancient knowledge through thorough scientific investigation, aiming to enhance the global acceptance of Ayurveda.

31) Water:
Water serves as a medium in various preparations and analytical techniques mentioned in the study, such as Zeta potential and particle size analysis. Its role is crucial in ensuring that the components of Shatpala Gandhaka Churna are effectively processed and measured for optimal performance in medicinal contexts.

32) Bhaishajya (Bhaisajya):
Bhaishajya refers to the medicinal formulations in Ayurveda that are prepared from various natural ingredients. The term emphasizes the art and science of creating effective herbal medicines, aligning with the study's focus on the production and analysis of Shatpala Gandhaka Churna for therapeutic purposes.

33) Shrinivasa (Shri-nivasa, Srinivasa):
Srinivasa alludes to Sri Srinivasa Ayurvedic Pharmacy where critical components of Shatpala Gandhaka Churna are procured and studied. This underscores the blend of traditional practice and contemporary research in evaluating the herbal formulations' properties and therapeutic value while preserving the Ayurvedic heritage.

34) Toxicity:
Toxicity involves evaluating the safety profile of the components in Shatpala Gandhaka Churna. The study aims to address concerns regarding toxic elements through rigorous analyses to ensure that the formulation is safe for therapeutic use, reflecting Ayurveda's principles of ensuring well-being through safe practices.

35) Kalpana:
Kalpana denotes the preparation or formulation aspects within Ayurveda. This term emphasizes the systematic approach when creating medicinal compounds like Shatpala Gandhaka Churna, as it merges ancient techniques and modern understanding to provide therapeutic solutions that are historically grounded yet scientifically validated.

36) Quality:
Quality pertains to the standards of efficacy, safety, and purity established for the components in Shatpala Gandhaka Churna. The study undertakes comprehensive analyses using modern techniques to ensure the drug maintains high quality, reinforcing the importance of consistent quality control in Ayurvedic medicine.

37) Silver:
Silver, a trace element analyzed in the study, indicates the presence of various minerals within Shatpala Gandhaka Churna. Although present in minute quantities, it may contribute to the overall therapeutic properties of the formulation, showcasing the balance of herbal and mineral constituents traditionally esteemed in Ayurvedic treatments.

38) Filling (Filled):
Filling refers to the process of encapsulating the powdered Shatpala Gandhaka Churna into capsules. This step is crucial for preserving the formulation’s integrity and ensuring accurate dosing, making herbal preparations more adaptable for contemporary medical use and facilitating convenient patient administration.

39) Ghi (Ghee):
Ghee, or clarified butter, is used as a medium in the purification process (Shodhana) for some ingredients in Shatpala Gandhaka Churna. It plays a significant role in enhancing the bioavailability of the formulation and exemplifies Ayurveda's emphasis on using natural substances for therapeutic efficacy.

40) Milk:
Milk is employed in the preparation process of Shatpala Gandhaka Churna during the purification phase of certain ingredients. Its use embodies the Ayurvedic principle of combining various natural substances to enhance the therapeutic qualities of herbal formulations, demonstrating traditional methods to detoxify and purify vital components.

41) Shuddhagandhaka (Suddhagandhaka, Shuddha-gandhaka):
Shuddha-gandhaka refers to purified sulfur, a major component in Shatpala Gandhaka Churna. The purification process ensures that it is free from impurities, which is crucial for maintaining safety and efficacy in Ayurvedic medicine. This highlights the importance of quality control in preparing medicinal compounds.

42) Vijayawada (Vijayavada, Vijaya-vada):
Vijayawada is the location where Gandhaka used in the formulation is sourced. The significance of geographical sourcing emphasizes how local ingredients are integral to crafting authentic Ayurvedic formulations. This connection demonstrates the local heritage that enriches the authenticity of traditional medicinal practices in India.

43) Discussion:
Discussion refers to the section where the implications of the study's findings are analyzed and interpreted. It draws connections between traditional Ayurvedic practices and modern scientific techniques, exploring how these findings impact the understanding, acceptance, and application of herbal formulations in contemporary healthcare.

44) Knowledge:
Knowledge refers to the insights gained through the analytical study of Shatpala Gandhaka Churna. This encompasses both traditional Ayurvedic wisdom and modern scientific understanding, creating a comprehensive view that strengthens the validation of Ayurvedic practices within the context of evidence-based medicine.

45) Substance:
Substance refers to the individual components and materials analyzed in Shatpala Gandhaka Churna. This term emphasizes the importance of understanding each ingredient's properties and potential effects in formulations, ensuring that their combined therapeutic qualities are maximized for patient care and treatment outcomes.

46) Container:
Container signifies the sterile storage method employed for Shatpala Gandhaka Churna capsules. Proper containment is crucial to maintaining the formulation's integrity, preventing contamination, and ensuring that the potency and safety of the herbal medicine persist during storage until administration.

47) Madhyama:
Madhyama refers to the intermediate section of the Sharangadhara Samhita, serving as a reference for processing certain herbs and minerals. This highlights the importance of classical texts in guiding the methods employed for purification and formulation, bridging traditional practices and modern scientific methodologies effectively.

48) Medicine:
Medicine encompasses the broader field of therapeutic interventions, including herbal formulations like Shatpala Gandhaka Churna. This term signifies the objective of the study: to validate and improve traditional Ayurvedic medicines to align with contemporary healthcare standards and enhance their acceptance in mainstream medicine.

49) Samhita:
Samhita refers to the ancient Ayurvedic texts that contain knowledge on herbal medicine, formulations, and healing practices. Understanding how classic literature informs the preparation of Shatpala Gandhaka Churna demonstrates the foundational role these texts play in sustaining and evolving traditional Ayurvedic knowledge within modern contexts.

50) Venkata (Vemkata):
Venkata refers to Venkata Subbaiah K., one of the co-authors of this research article. His association with the study illustrates the collaborative effort among scholars in Ayurveda to enhance the understanding and standardization of Ayurvedic formulations through scientific analysis and validation.

51) Kushtha (Kustha):
Kushtha refers to various skin diseases mentioned in Ayurvedic texts, highlighting the therapeutic uses of Shatpala Gandhaka Churna in treating skin-related ailments. Understanding these traditional applications underlines the relevance of ancient knowledge in addressing contemporary health issues through evidence-based methodologies.

52) Chennai:
Chennai is a major city in India, serving as a source for ingredients like Triphala and other herbs used in the formulation of Shatpala Gandhaka Churna. This signifies the significance of geographic diversity in sourcing natural materials, an essential aspect of crafting authentic Ayurvedic medicines based on traditional principles.

53) Pouring:
Pouring is part of the Shodhana process for purification, especially for ingredients like Gandhaka, typically involving the transfer of melted substances through specific mediums like ghee and milk. This process underscores the importance of meticulous preparation methods in Ayurveda to ensure safety and effectiveness in herbal formulations.

54) Vaidya:
Vaidya refers to an Ayurvedic practitioner or physician, emphasizing the role of traditional healers in formulating and prescribing Ayurvedic medicines like Shatpala Gandhaka Churna. The term underscores the traditional wisdom held by practitioners that integrates herbal knowledge with patient care in managing health conditions.

55) Mahila:
Mahila in this context represents the 'Women,' specifically referring to Padmavathi Mahila University where some analyses were conducted. This reflects an emphasis on institutional involvement in enhancing research capabilities and underlines the educational institutions' role in advancing Ayurvedic science and technology.

56) Andhra (Amdhra):
Andhra refers to Andhra Pradesh, a state in India where the research on Shatpala Gandhaka Churna takes place. The designation emphasizes the region's rich cultural and medicinal heritage, highlighting the integration of traditional practices with modern scientific approaches in the study of Ayurveda.

57) Ishtika (Istika):
Istika is a reference to a type of fine powder used during the Shodhana process of purification, particularly for Bhallataka. Its use signifies the importance of specific materials and techniques in Ayurveda to ensure that raw ingredients are thoroughly processed for safe therapeutic applications.

58) Khanda (Khamda):
Khanda translates to 'section' in Sanskrit, specifically referring to segments in Ayurvedic texts such as the Madhyama Khanda of the Sharangadhara Samhita. This demonstrates the crucial role of organized knowledge in guiding Ayurvedic practices and understanding the medicinal properties of formulations like Shatpala Gandhaka Churna.

59) Kadapa:
Kadapa is another city in Andhra Pradesh where some research activities may have occurred. The local geographical context highlights the region's contribution to sourcing and studying herbal ingredients, enhancing the understanding of traditional medicinal practices deeply rooted within specific cultural frameworks.

60) Ratna:
Ratna translates to 'jewel' in Sanskrit, commonly used in Ayurveda to denote highly valuable substances, particularly herbal or mineral ingredients. The reference to Ratna emphasizes the esteemed quality of components in Shatpala Gandhaka Churna, signifying their significance in traditional healing and the quest for purity in Ayurveda.

61) Glass:
Glass refers to the containers used for storing and preserving Shatpala Gandhaka Churna capsules. The highlight on using glass underscores the importance of maintaining purity and preventing contamination, which is essential in ensuring the integrity and efficacy of Ayurvedic formulations up to the point of use.

62) Roga:
Roga refers to diseases or ailments in Ayurveda, emphasizing the therapeutic applications of Shatpala Gandhaka Churna in treating skin diseases (Kushtha). Understanding the health conditions addressed by the formulation highlights the integration of traditional knowledge with evidence-based practices in addressing patient health challenges.

63) Yoga:
Yoga refers to practices that intertwine physical and spiritual disciplines prevalent in Ayurveda. The term emphasizes holistic approaches to health that include the use of formulations like Shatpala Gandhaka Churna designed not only for physical healing but also promoting overall wellness in alignment with Ayurvedic principles.

64) Yogi (Yogin):
Yogi signifies an individual practicing yoga, representing a holistic approach to health and wellness that reflects Ayurvedic principles. The association underscores the integration of yoga with the use of Ayurvedic formulations, creating a comprehensive strategy for achieving balance between mind, body, and spirit in health practices.

65) Jute:
Jute refers to the natural fiber often used in Ayurvedic preparations, specifically for wrapping or containing raw materials during processing. The use of jute exemplifies the traditional practices that involve sustainable materials, aligned with Ayurveda’s principles of utilizing natural resources for preparation and therapeutic methods.

66) Mula:
Mula refers to the root part of plants, which is often utilized in Ayurvedic formulations. In Shatpala Gandhaka Churna, various roots may be included to enhance the medicinal properties, emphasizing the importance of whole plant usage and the holistic philosophy that underpins Ayurvedic medicine.

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Analytical study, Therapeutic efficacy, Pharmaceutical procedure, Safety and toxicity, UV-Spectroscopy, Scanning Electron Microscopy (SEM), Modern Analytical Parameters, Fourier transform Infrared Spectroscopy (FTIR), X-ray Diffraction (XRD), Zeta Potential (ZP), Inductively Coupled Plasma – Optical Emission Spectrometry (ICP-OES), Nano Size.

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