Screening antioxidant activity of phytosterol from Holoptelea integrifolia.
Journal name: World Journal of Pharmaceutical Research
Original article title: Screening of isolated phytosterol from leaves of holoptelea integrifolia (roxb) planch for antioxidant activity
The WJPR includes peer-reviewed publications such as scientific research papers, reports, review articles, company news, thesis reports and case studies in areas of Biology, Pharmaceutical industries and Chemical technology while incorporating ancient fields of knowledge such combining Ayurveda with scientific data.
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Ranjana Sharma, Sudhir Singh Gangwar, Amita Tilak, R. N. Thakur and Ravindra C. Sutar
World Journal of Pharmaceutical Research:
(An ISO 9001:2015 Certified International Journal)
Full text available for: Screening of isolated phytosterol from leaves of holoptelea integrifolia (roxb) planch for antioxidant activity
Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research
Doi: 10.20959/wjpr201819-13707
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Summary of article contents:
Introduction
Holoptelea integrifolia (Roxb.) Planch, commonly known as Indian Elm, has a long history of usage in traditional medicine practices. The study conducted by Sharma et al. aimed to evaluate the antioxidant activity of phytosterols isolated from the leaves of H. integrifolia. Given the implication of reactive oxygen species (ROS) in various degenerative diseases and the importance of antioxidants in counteracting oxidative stress, it was vital to assess the plant's potential benefits in this regard. The research primarily focused on the total reduction capability of the isolated phytosterol (HIIP) from petroleum ether extracts.
Antioxidant Activity of HIIP
The antioxidant activity of the isolated phytosterol (HIIP) was assessed using a method known as Total Reduction Capability. The results indicated a dose-dependent increase in absorbance, which correlates with enhanced antioxidant activity. This suggests that HIIP effectively neutralizes free radicals, thereby showcasing its potential as a natural antioxidant agent. The investigation highlighted the importance of HIIP in medical applications, providing a scientific basis for the traditional claims of antioxidant properties associated with H. integrifolia.
Phytochemical Composition
Preliminary phytochemical investigations of the petroleum ether extract of H. integrifolia leaves revealed the presence of various compounds, including steroids, terpenoids, alkaloids, glycosides, flavonoids, proteins, tannins, and carbohydrates. Each of these constituents contributes to the overall pharmacological effects of the plant. This rich phytochemical profile underscores the relevance of H. integrifolia in traditional medicine, where it has been utilized to treat various ailments. The documented medicinal properties are corroborated by the detected presence of these bioactive compounds.
Methodology of Isolation and Evaluation
The study employed a systematic methodology for isolating the HIIP from the leaves of H. integrifolia. Using high-performance thin-layer chromatography (HPTLC), approximately 4.8% yield of the phytosterol was obtained from the petroleum ether extract. The extraction process involved multiple steps, including drying, size reduction, and solvent extraction, to ensure the integrity and bioactivity of the compounds. The rigor in methodology provided a reliable means to evaluate the biological activity of the isolated phytosterol effectively.
Conclusion
The findings from the study affirm that HIIP from H. integrifolia demonstrated significant antioxidant activity through the Total Reduction Capability assay. This supports the ethnomedicinal use of the plant and suggests that its phytosterols may play a crucial role in combating oxidative stress-related diseases. The evidence gathered in this research encourages further exploration of H. integrifolia for its potential therapeutic applications, aiming to validate its traditional uses in treating various health issues. The combination of historical knowledge and modern scientific scrutiny presents avenues for future studies in natural products and their effective use in pharmacotherapy.
FAQ section (important questions/answers):
What is the main objective of the study on Holoptelea integrifolia?
The main objective of the study was to evaluate the antioxidant activity of isolated phytosterol from the leaves of Holoptelea integrifolia using the Total Reduction Capability method.
Which method was used to isolate phytosterol from Holoptelea integrifolia?
Phytosterol was isolated using preparative Thin Layer Chromatography (TLC) from the petroleum ether extract of Holoptelea integrifolia leaves.
What phytochemical constituents were found in the leaf extract?
Preliminary phytochemical investigation revealed steroids, terpenoids, alkaloids, glycosides, flavonoids, proteins, tannins, and carbohydrates in the petroleum ether extract of the leaves.
How was the antioxidant activity of HIIP measured?
The antioxidant activity was measured using the Total Reduction Capability method, assessing the reduction capacity of different concentrations of HIIP compared to Vitamin C.
What were the results of the total reduction capability test for HIIP?
HIIP showed a dose-dependent increase in absorbance, indicating significant antioxidant activity with higher concentrations resulting in greater reducing power.
What traditional uses are associated with Holoptelea integrifolia?
Traditionally, Holoptelea integrifolia has been used for treating inflammation, gastritis, diabetes, rheumatism, and other health conditions.
Glossary definitions and references:
Scientific and Ayurvedic Glossary list for “Screening antioxidant activity of phytosterol from Holoptelea integrifolia.”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.
1) Activity:
Activity in this context refers to the biological effect of the isolated phytosterol compound derived from Holoptelea integrifolia. It indicates how effectively the compound can perform its role as an antioxidant, potentially influencing various physiological functions and contributing to health benefits by counteracting oxidative stress in cells.
2) India:
India is the geographical location where Holoptelea integrifolia is commonly found and utilized in traditional medicine. The country's rich biodiversity includes numerous medicinal plants, and India serves as a focal point for research into herbal remedies and their applications in treating various health conditions and promoting wellness.
3) Phytochemical:
Phytochemicals are naturally occurring compounds found in plants that contribute to their color, flavor, and disease resistance. In the context of this study, phytochemicals such as flavonoids and steroids are being researched for their potential health benefits, especially concerning their antioxidant properties and therapeutic effects against diseases.
4) Science (Scientific):
Scientific refers to the systematic approach employed in the research and analysis presented within the study. It highlights the methods, experiments, and conclusions drawn based on empirical evidence, reinforcing the credibility of the findings related to the antioxidant activity of the phytosterol isolated from the plant.
5) Flavonoid:
Flavonoids are a class of phytochemicals noted for their antioxidant properties and are commonly found in many plants, including Holoptelea integrifolia. Their significance lies in their ability to combat oxidative stress, offering protective benefits against various diseases, such as cardiovascular disorders and cancer.
6) Study (Studying):
The study encompasses the research carried out to evaluate the antioxidant properties of isolated phytosterols from Holoptelea integrifolia. It involves various methodologies, including extraction and testing for biological activity, aimed at establishing the therapeutic efficacy of this plant material within a scientific framework.
7) Pharmacology:
Pharmacology is the branch of medicine that deals with the study of drug action on biological systems. The relevance here is the exploration of how phytosterols, as potential pharmaceutical agents, can impact health outcomes, particularly their role in treating conditions influenced by oxidative stress.
8) Inflammation:
Inflammation is a biological response to harmful stimuli, including pathogens, damaged cells, and irritants. In the context of the study, the antioxidant properties of Holoptelea integrifolia's phytosterols may help manage inflammation-related conditions, providing a basis for its traditional therapeutic uses.
9) Maharashtra (Maharastra, Maha-rashtra):
Maharashtra is a state in India where one of the institutions involved in the study is located. This emphasizes regional contributions to medicinal research and the cultivation of local flora with potential health benefits, enhancing the relevance of traditional practices in modern pharmacological studies.
10) Arrangement:
Arrangement refers to the organization of plant morphological features that help in identifying and authenticating Holoptelea integrifolia. This aspect is crucial for ensuring accurate botanical classification and understanding the relationships among species within the Ulmaceae family for research and medicinal applications.
11) Ahmednagar:
Ahmednagar is a district in Maharashtra, India, relevant for the geographical and cultural context of the research. It is significant as the collection site for the Holoptelea integrifolia leaves, linking local biodiversity to traditional medicine and scientific inquiry into its medicinal properties.
12) Samjivani (Sanjivani):
Sanjivani refers to the college of pharmaceutical education involved in the research. It signifies the collaboration of academic institutions in advancing pharmacological studies, enhancing the understanding of traditional medicine, and validating the medicinal potentials of plants through scientific investigation.
13) Education:
Education is emphasized through the role of institutions like Sanjivani College, which contribute to advancing knowledge in pharmacology and herbal medicine. It underpins the importance of training and research in developing effective treatments derived from traditional plant-based remedies.
14) Medicine:
Medicine encompasses the field focused on health care and treatment of diseases. This study highlights the intersection of traditional medicine and modern pharmacological research, investigating the therapeutic potential of plant-derived compounds in treating various health conditions through scientific methodologies.
15) Water:
Water in this context may refer to its role as a solvent in various extraction processes used in the study. It is a crucial medium for biological reactions, impacting the efficacy of phytochemical extraction and demonstrating the importance of suitable solvents in pharmacological research.
16) Table:
Table refers to the presentation of data in a structured format within the study. It is used to summarize findings, such as the total reduction capability results, facilitating clarity and comparison of the antioxidant activities of the isolated phytosterol and standard compounds.
17) Post:
Post in this context may imply an official communication or document related to the research. It symbolizes the importance of disseminating research findings to the broader scientific community, ensuring that valuable insights from the study reach relevant stakeholders and the public.
18) Human body:
The human body is the biological system that the compiled research aims to impact through the potential therapeutic benefits of the isolated phytosterol. Understanding the antioxidant activity’s effects on human health provides context for studying medicinal plants like Holoptelea integrifolia.
19) Pharmacotherapy:
Pharmacotherapy is the treatment of disease through the administration of drugs. This study explores the implications of phytosterols as natural compounds that might serve as alternatives or complements to conventional pharmacotherapy in managing oxidative stress-related diseases.
20) Pharmacological:
Pharmacological refers to the principles related to drug action and therapeutic outcomes. The study delves into the pharmacological potential of phytosterols isolated from Holoptelea integrifolia, investigating their antioxidant properties and their implications for developing herbal remedies and therapies.
21) Transformation (Transform, Transforming):
Transformation refers to the biochemical processes involving changes in chemical species, such as the reduction of Fe3+ to Fe2+ in the antioxidant assays presented in the study. It highlights the mechanisms behind the antioxidant activity of HIIP and its potential to mitigate oxidative stress.
22) Dysmenorrhea (Dysmenorrhoea):
Dysmenorrhea is a medical condition characterized by painful menstruation. The mention within the study underscores traditional uses of Holoptelea integrifolia in alleviating various ailments, revealing potential insights into its effectiveness as a natural remedy for menstrual-related discomforts.
23) Tirunelveli:
Tirunelveli is a district in Tamil Nadu where Holoptelea integrifolia leaves were collected for the study. Its significance lies in highlighting local biodiversity, the importance of ethnobotanical knowledge, and the contributions of regional flora to traditional healthcare practices.
24) Performance:
Performance in this context relates to the effectiveness of the isolated phytosterols in exhibiting antioxidant activity as measured in experiments. Understanding their performance helps evaluate the phytochemical's potential in various therapeutic applications and validates traditional medicinal claims.
25) Observation:
Observation refers to the process of systematically noting and recording data during scientific experiments, a critical aspect of the study. Observations provide the foundation for drawing conclusions about the antioxidant properties of phytosterols and contribute to the overall reliability of the research.
26) Discussion:
Discussion in a research context involves analyzing results, comparing findings with previous studies, and drawing conclusions based on the data. It provides an opportunity to explore the implications of the study's results and suggests future research directions regarding Holoptelea integrifolia.
27) Tamilnadu (Tamil-nadu):
Tamil Nadu is a state in India known for its rich biodiversity, including medicinal plants like Holoptelea integrifolia. The region's unique flora contributes to traditional medicine practices, increasing the relevance of studying local species for potential pharmacological benefits.
28) Attacking:
Attacking refers to the harmful interaction between free radicals and biological molecules, which can lead to cellular damage. This concept is foundational in understanding oxidative stress and the role of antioxidants in protecting cells from such detrimental effects, underlining the importance of the study.
29) Vomiting:
Vomiting is a symptom that may arise from various gastrointestinal disturbances. The inclusion of vomiting signifies the traditional medicinal uses of Holoptelea integrifolia in treating such conditions, indicating the need for scientific validation of its effectiveness in alleviating these symptoms.
30) Swelling:
Swelling refers to an increase in size or volume of body parts, often due to inflammation. The use of Holoptelea integrifolia in treating swellings emphasizes its traditional value in herbal medicine, warranting scientific investigation into its anti-inflammatory and therapeutic properties.
31) Ranjana (Ramjana):
Ranjana refers to one of the authors of the study. Her involvement signifies the collaborative nature of scientific research and highlights the contributions of various individuals toward advancing knowledge and understanding of the therapeutic properties of traditional medicinal plants.
32) Habitat:
Habitat refers to the natural environment where Holoptelea integrifolia grows. Understanding its habitat is crucial for conservation efforts, sustainable use of the plant, and recognizing the ecological significance of preserving biodiversity that supports traditional medicine practices.
33) Leprosy:
Leprosy, also known as Hansen's disease, is an infectious disease affecting the skin and nerves. The traditional use of Holoptelea integrifolia in treating such ailments highlights the need for scientific inquiry into its effectiveness, expanding the understanding of its therapeutic potential.
34) Disease:
Disease refers to pathological conditions affecting the body's normal functioning. This study aims to explore the potential of Holoptelea integrifolia extracts in combating diseases linked to oxidative stress, emphasizing the importance of traditional medicinal knowledge in addressing health issues.
35) Species:
Species denotes the category of organisms that share common characteristics. Identifying Holoptelea integrifolia as a specific species allows researchers to focus on its unique properties, thereby advancing scientific knowledge of its medicinal benefits and their applications in the treatment of various ailments.
36) Family:
Family refers to the taxonomic classification in biology that groups related species. Holoptelea integrifolia belongs to the Ulmaceae family, which aids researchers in understanding its ecological relationships and potential shared characteristics with other species used in traditional medicine.
37) Cancer:
Cancer is a serious disease characterized by uncontrolled cell growth. The investigation of antioxidant properties in Holoptelea integrifolia is relevant, as antioxidants may play a role in preventing oxidative damage that could lead to cancer, thus emphasizing its potential as a preventative herbal remedy.
38) Bitter:
Bitter describes a taste characteristic of some medicinal plants, including Holoptelea integrifolia. The bitter flavor is often associated with various therapeutic effects, reinforcing the plant's traditional use in medicine for conditions like digestive disorders, and enhancing its profile as a potential remedy.
39) Sharman (Sarma, Sarman, Sharma):
Sharma refers to one of the co-authors of the study. The mention of authors emphasizes the collaborative aspect of scientific research and highlights the contributions of individuals toward exploring the health benefits of traditional plants through rigorous investigation.
40) Indian:
Indian refers to the cultural and geographical identity relating to the context of the study. This highlights the traditional uses of Holoptelea integrifolia in Indian medicine, underlining the significance of indigenous knowledge in the development of alternative therapies based on local flora.
41) Botany:
Botany is the branch of biology that studies plants. Its relevance in this study underscores the importance of understanding the botanical characteristics of Holoptelea integrifolia to explore its medicinal properties and validate traditional claims through scientific research.
42) Glass:
Glass may refer to the equipment used in laboratory procedures, such as glassware for conducting experiments. Proper handling of glass instruments is crucial in pharmacological research for maintaining accuracy and safety during chemical analyses and the extraction of phytochemicals.
43) Tilak:
Tilak refers to the co-author of the study, contributing to the collaborative aspect of research. The presence of multiple authors highlights the interdisciplinary approach in exploring the medicinal properties of Holoptelea integrifolia and furthering scholarly communication and findings.
44) Field:
Field may refer to the agricultural environment or study area where Holoptelea integrifolia is grown. Understanding the context of cultivation is essential for assessing the quality of plant material and the environmental factors influencing its medicinal properties, bolstering research significance.
45) Earth:
Earth refers to the planet hosting diverse ecosystems teeming with medicinal plants. Highlighting this aspect connects the research of Holoptelea integrifolia to the global appreciation of traditional medicine, emphasizing the importance of conservation and sustainable practices in plant usage.
46) Tamil:
Tamil refers to the linguistic and cultural identity relevant to the state of Tamil Nadu, where Holoptelea integrifolia is found. The cultural significance of the plant within the Tamil community underscores the regional richness of traditional medicine and its relevance in scientific studies.
47) Acrid:
Acrid describes a sharp and bitter taste characteristic of some medicinal plants, including Holoptelea integrifolia. This taste is often associated with therapeutic benefits, and understanding its presence aids in contextualizing the plant's traditional uses and effectiveness in treating various ailments.
48) Nadu:
Nadu, as part of Tamil Nadu, signifies the regional identity and cultural context where Holoptelea integrifolia is prevalent. This identification underscores the traditional practices of using local flora in medicine, emphasizing the importance of regional knowledge in scientific exploration.
49) Line:
Line refers to the specific scientific or traditional knowledge paths being followed in research. In studies of Holoptelea integrifolia, it denotes the methods and findings that connect traditional uses with scientific validation, aiding in the discovery of new therapeutic potentials.
50) Worm:
Worm in this context usually refers to intestinal worms, which can be a target for traditional remedies. Holoptelea integrifolia's traditional applications for anthelmintic properties underlie the necessity of scientific research into its effectiveness against parasitic infections, spotlighting ethnobotanical relevance.
51) Drug:
Drug refers to any substance used in the diagnosis, treatment, or prevention of disease. The focus on the pharmacological properties of Holoptelea integrifolia illustrates its potential role as a natural drug or therapeutic agent, stemming from traditional knowledge into scientifically validated practices.
Other Science Concepts:
Discover the significance of concepts within the article: ‘Screening antioxidant activity of phytosterol from Holoptelea integrifolia.’. Further sources in the context of Science might help you critically compare this page with similair documents:
Medicinal properties, Traditional medicine, Therapeutic evaluation, Antioxidant activity, Oxidative stress, Reactive oxygen species, Phytochemical constituents, Molecular weight, Oxidative damage, Dose-dependent, Petroleum ether extract, Inflammatory response, In vitro model.