Standardisation of one of traditional ‘dashmula’
shalparni (desmodium gangeticum linn. dc. family: leguminosae) through phytochemical and histological evaluation
Journal name: World Journal of Pharmaceutical Research
Original article title: Standardisation of one of traditional ‘dashmula’
The WJPR includes peer-reviewed publications such as scientific research papers, reports, review articles, company news, thesis reports and case studies in areas of Biology, Pharmaceutical industries and Chemical technology while incorporating ancient fields of knowledge such combining Ayurveda with scientific data.
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Subtitle: shalparni (desmodium gangeticum linn. dc. family: leguminosae) through phytochemical and histological evaluation
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Attarde D.L., Dr. Pal S.C. and Dr. Bhambar R.S.
World Journal of Pharmaceutical Research:
(An ISO 9001:2015 Certified International Journal)
Full text available for: Standardisation of one of traditional ‘dashmula’
Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research
Doi: 10.20959/wjpr20172-7771
Copyright (license): WJPR: All rights reserved
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Summary of article contents:
Introduction
The research article focuses on the standardization of Desmodium gangeticum (L.) DC, also known as Shalparni, a significant herb utilized in traditional Ayurvedic medicine, specifically as one of the constituents of 'Dashmoola'. The study explores the plant's phytochemical properties, anatomical characteristics, and utilizes advanced techniques such as Scanning Electron Microscopy (SEM) and High-Performance Thin-Layer Chromatography (HPTLC) for botanical identification. Following the guidelines set by the World Health Organization (WHO), the study aims to establish a set of standards for the identification and quality assessment of this herbal material.
Phytochemical Screening
The exploration of the chemical composition of Desmodium gangeticum revealed the presence of various bioactive compounds. The phytochemical screening conducted through different solvent extracts indicated that the plant contains steroids, flavonoids, alkaloids, carbohydrates, and tannins. Notably, the ethyl acetate extract exhibited seven distinct zones upon HPTLC analysis, suggesting a rich profile of phytochemicals that may contribute to its pharmacological effects. Furthermore, Genistein, a flavonoid identified in the extracts, has been marked as a significant biomarker due to its numerous health benefits, including roles in osteoporosis and cardiovascular health.
Microscopic and Histological Evaluation
The research extensively examined the microscopic characteristics of the stem of Desmodium gangeticum using both trinocular microscopy and SEM. The transverse sections revealed distinct histological features, such as the structure of the pith, xylem, and phloem, along with the presence of collenchyma and various types of trichomes. This comprehensive histological evaluation facilitated the identification of key structural parameters of the plant, reinforcing the botanical authenticity and aiding in the development of quality control measures for herbal formulations involving this species.
HPTLC Fingerprinting and Stability Analysis
HPTLC fingerprinting played a crucial role in the study, particularly in identifying Genistein as a marker compound in the ethyl acetate extract. The chromatographic analysis demonstrated good resolution with marked Rf values, confirming the consistency and stability of the extract over time. Stability studies indicated that the chemical composition remained unchanged during chromatography, ensuring reliability in identification and standardization processes. This aspect is essential for the utilization of Desmodium gangeticum in various herbal preparations, as it supports dosage formulation and efficacy evaluations.
Conclusion
The standardization study of Desmodium gangeticum provides critical insights into the phyto-chemical profile and microscopical characteristics of this important medicinal plant. The identification of Genistein as a biomarker alongside detailed histological evaluation and robust chromatographic techniques enhances the credibility of the research, underscoring the importance of quality control in herbal medicine. As herbal products gain increasing prominence in healthcare, establishing such standards is essential for ensuring safety, efficacy, and consistency in therapeutic applications.
FAQ section (important questions/answers):
What is Desmodium gangeticum and its traditional use in Ayurveda?
Desmodium gangeticum, known as Salparni, is a traditional Ayurvedic herb used in various formulations for treating ailments like asthma, disorders, and as a bitter tonic.
What techniques were used for the study of Desmodium gangeticum?
The study utilized Scanning Electron Microscopy (SEM) for histological evaluation and High-Performance Thin Layer Chromatography (HPTLC) for phytochemical fingerprinting.
What were the main findings from the phytochemical screening?
Phytochemical screening revealed the presence of steroids, alkaloids, tannins, and flavonoids in the various extracts of Desmodium gangeticum.
How was the stability of the ethyl acetate extract tested?
Stability tests checked the ethyl acetate extract over time in solution and chromatography, showing consistent chromatograms without changes.
What is the significance of Genistein in the study?
Genistein, identified as a key isoflavone in the extracts, can serve as a biomarker for the standardization of Desmodium gangeticum due to its pharmacological properties.
What are the key physical parameters measured in the study?
Key physical parameters included moisture content, total ash, extractive values, and crude fiber content, aligning with standards from the Ayurvedic Pharmacopoeia.
Glossary definitions and references:
Scientific and Ayurvedic Glossary list for “Standardisation of one of traditional ‘dashmula’”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.
1) Powder:
Powder refers to the finely ground form of the dried plant materials used in herbal medicine. In the context of the study, the powder of Desmodium gangeticum stems is analyzed for its physical and chemical properties, which aids in the characterization and standardization of herbal formulations.
2) Phytochemical:
Phytochemicals are the bioactive compounds found in plants that contribute to their medicinal properties. The study focuses on identifying these phytochemicals in Desmodium gangeticum, such as sterols, flavonoids, and alkaloids, to establish quality control parameters for traditional herbal medicines.
3) Flavonoid:
Flavonoids are a class of phytochemicals known for their antioxidant and anti-inflammatory effects. Genistein, a specific flavonoid identified in the study, serves as a biomarker for standardization of Desmodium gangeticum extracts, highlighting its potential therapeutic roles in various health issues.
4) Water:
Water is a crucial solvent used in the extraction and analysis of phytochemicals from plant materials. In the study, the water-soluble extractive values of Desmodium gangeticum are measured, which provides insights into the plant’s medicinal properties and its efficacy in herbal remedies.
5) Study (Studying):
The study refers to the systematic investigation undertaken to standardize Desmodium gangeticum as a raw material for herbal formulations. It combines various methods, including phytochemical screening, microscopy, and chromatographic techniques, to ensure quality and identify key bioactive compounds.
6) Table:
Tables in scientific literature present organized data succinctly, facilitating comprehension. The study includes tables that summarize physical parameters, phytochemical screening results, and HPTLC fingerprinting, enabling quick reference and comparison with standard values outlined in the Ayurvedic Pharmacopoeia.
7) Desmodium gangeticum:
Desmodium gangeticum is a leguminous plant known for its medicinal uses in Ayurveda. This herb, also called Shalparni, is subjected to various analyses in the study to understand its pharmacological properties, quality control, and potential as a standard herbal ingredient.
8) Nashik:
Nashik is a city in Maharashtra, India, known for its rich biodiversity and traditional medicine practices. The study's samples of Desmodium gangeticum were collected from the Igatpuri forest in the Nashik district, providing valuable regional information on the plant’s habitat and availability.
9) Quality:
Quality refers to the standard of the herbal products being evaluated in the study. Ensuring the quality of Desmodium gangeticum through various evaluations, including physicochemical and phytochemical testing, is crucial for its efficacy and safety in medicinal applications.
10) India:
India is a country with a long history of herbal medicine and Ayurveda. The study emphasizes the importance of standardizing traditional herbal formulations like Desmodium gangeticum to align with global quality and safety standards for herbal medicine in India.
11) Hair:
Hair in the context of this study may refer to the microscopic structures, such as trichomes, present on the plant surface. The evaluation of these features through microscopy helps in the identification and authentication of Desmodium gangeticum as a medicinal herb.
12) Shalparni (Salparni):
Salparni is the common name for Desmodium gangeticum used in various regional languages. The name reinforces the plant's cultural and medicinal significance in Ayurveda, where it is often included in formulations aimed at treating a variety of health issues.
13) Medicine:
Medicine encompasses the study and practice of diagnosing and treating illnesses, including the use of herbal formulations. The research aims to establish Desmodium gangeticum as a standardized medicinal ingredient, promoting its safe use and effectiveness in treating various health conditions.
14) Activity:
Activity in this study refers to the biological or pharmacological effects produced by the compounds found in Desmodium gangeticum. By identifying these activities, the researchers aim to correlate phytochemical presence with the herb's traditional uses and therapeutic potentials.
15) Mahatman (Mahatma, Maha-atman):
Mahatma Gandhi Vidyamandir’s Pharmacy College in Nashik is where part of this research is conducted. It plays a vital role in the study by providing a framework for academic investigation into botanicals like Desmodium gangeticum, aligning education with practical applications in pharmacy.
16) Line:
Line refers to the representation of analytical results in chromatography, where the positioning of spots or bands indicates the presence of specific compounds. The study emphasizes obtaining well-defined lines during HPTLC fingerprinting for accurate identification of phytochemical constituents.
17) Drug:
Drug refers to any substance used to diagnose, cure, mitigate, treat, or prevent disease. The study explores Desmodium gangeticum as a potential drug source by analyzing its phytochemical constituents and their possible pharmacological effects, linking traditional uses to modern applications.
18) Pharmacological:
Pharmacological relates to the study of drug effects and mechanisms of action within biological systems. The research focuses on the pharmacological potential of phytochemicals in Desmodium gangeticum, emphasizing its therapeutic use in traditional medicine and the need for scientific validation.
19) Shaliparni (Saliparni, Shali-parni):
Shaliparni is another name for Desmodium gangeticum used in Sanskrit literature and traditional medicine. The nomenclature highlights the plant's historical significance and the continuity of its use in various Ayurvedic formulations across different cultures and languages.
20) Discussion:
Discussion involves analyzing and interpreting the results obtained from the study. The study includes a discussion section to evaluate the findings related to the quality, efficacy, and standardization of Desmodium gangeticum in correlation with its traditional medicinal uses.
21) Gitanaram:
Gitanaram is another regional name for Desmodium gangeticum in Telugu. The various names highlight the herb's widespread recognition and utilization across different Indian languages and cultures, reflecting its importance in traditional medicine practices throughout India.
22) Commerce:
Commerce relates to the trade of goods and services, including herbal medicines. The study's context reflects the increasing market demand for standardized herbal products like Desmodium gangeticum, emphasizing the need for quality assurance in the herbal medicine industry.
23) Sanskrit:
Sanskrit is the ancient language of India, rich in literature on Ayurveda and traditional medicine. The use of Sanskrit names for plants like Desmodium gangeticum illustrates the deep historical roots of herbal medicine in Indian culture and its ongoing relevance today.
24) Marathi:
Marathi is the regional language spoken in Maharashtra, India, where the study is conducted. The presence of Marathi names for Desmodium gangeticum emphasizes the plant's local significance and the cultural context of herbal medicine practices in the region.
25) Kalyani (Kalyanin):
Kalyani refers to RG Sapakal College of Pharmacy located in Kalyani Hills, Maharashtra. The collaboration between institutions in this study enhances academic research focused on herbal medicine, thereby contributing to the standardization and recognition of plants like Desmodium gangeticum.
26) Habitat:
Habitat refers to the natural environment where Desmodium gangeticum grows, particularly in the lower Himalayan regions and plains of India. Understanding its habitat is essential for conservation efforts and sustainable harvest practices within traditional herbal medicine.
27) Disease:
Disease refers to any abnormal condition affecting the body or mind, often treated with medicinal plants. The study examines the efficacy of Desmodium gangeticum in treating various diseases by analyzing its phytochemical content and traditional uses in herbal remedies.
28) Forest:
Forest denotes the natural ecosystem where Desmodium gangeticum can be found. The collection site in Igatpuri forest illustrates the importance of preserving forest habitats for biodiversity and the sustainable sourcing of valuable medicinal plants used in Ayurveda.
29) Family:
Family in botanical taxonomy classifies plants with shared characteristics. Desmodium gangeticum belongs to the Leguminosae family, which includes many important medicinal and agricultural plants, highlighting the ecological and economic significance of this family in both traditional and modern contexts.
30) Bitter:
Bitter refers to one of the basic tastes and is often related to the medicinal properties of plants. Many herbal remedies, including those derived from Desmodium gangeticum, often possess bitter flavors, which are traditionally believed to enhance digestion and health benefits.
31) Cotton:
Cotton is mentioned in the context of collaboration with the Central Institute for Research on Cotton Technology. The relationship to cotton highlights interdisciplinary research efforts that may enhance the understanding of plant-based resources, including medicinal herbs like Desmodium gangeticum.
32) Filling (Filled):
Filled may refer to the presence of certain compounds or structures within plant tissues identified during the study, such as ergastic contents like starch grains. Understanding these aspects contributes to the overall characterization of Desmodium gangeticum and its extracts.
33) Glass:
Glass is commonly used in laboratory settings for holding samples or conducting experiments. The use of glass slides for microscopy in this study emphasizes the importance of proper tools in the accurate analysis and documentation of Desmodium gangeticum's characteristics.
34) Hindi (Himdi):
Hindi is one of the major languages spoken in India. The study references the Hindi names for Desmodium gangeticum, which underscore the cultural significance of the plant across diverse linguistic regions, linking language with traditional medicinal knowledge.
35) Pune:
Pune is a prominent city in Maharashtra, where the Botanical Survey of India is located. The connection to Pune for authentication of Desmodium gangeticum herbarium reflects the organized effort for preserving and validating herbal plant materials used in traditional medicine.
36) Wall:
Wall may refer to the cellular walls observed in plant tissues during microscopic studies. The analysis of cell wall content is vital for understanding tissue structure and composition, which aids in authenticating the identity of Desmodium gangeticum.
37) Gold (Golden):
Gold refers to a method used in Scanning Electron Microscopy (SEM) for coating samples, enabling enhanced imaging quality. The use of gold coating in this study underscores the advanced techniques employed to analyze the morphological details of Desmodium gangeticum.
38) Tai:
Thai refers to the language and culture of Thailand, where regional names for Desmodium gangeticum may exist. Acknowledging names in multiple languages reflects the global appreciation and utilization of this plant in various traditional medicinal practices beyond India.
Other Science Concepts:
Discover the significance of concepts within the article: ‘Standardisation of one of traditional ‘dashmula’’. Further sources in the context of Science might help you critically compare this page with similair documents:
Desmodium gangeticum, Ayurvedic pharmacopoeia, Microscopic evaluation, Phytochemical screening, Scanning electron microscope, HPTLC fingerprinting, Extraction method, Physicochemical Parameter, Herbal formulation standardization.