Effect of b-sitosterol on HBx gene-transfected HepG2 cells.
Journal name: World Journal of Pharmaceutical Research
Original article title: The biological effect of b-sitosterol on hepatoma cell hepg2 transfected with hbx gene
The WJPR includes peer-reviewed publications such as scientific research papers, reports, review articles, company news, thesis reports and case studies in areas of Biology, Pharmaceutical industries and Chemical technology while incorporating ancient fields of knowledge such combining Ayurveda with scientific data.
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Original source:
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Ammar M. Lubeiny, WuYun Li, Jin Zhen, Tang Zhang, M. A. Hassan, Yi Qin, Wei Liu, Xu Lin and Elshaikh A. Elobied
World Journal of Pharmaceutical Research:
(An ISO 9001:2015 Certified International Journal)
Full text available for: The biological effect of b-sitosterol on hepatoma cell hepg2 transfected with hbx gene
Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research
Doi: 10.20959/wjpr20172-7792
Download the PDF file of the original publication
Summary of article contents:
Introduction
Hepatitis B virus (HBV) is a major global health concern, frequently leading to hepatocellular carcinoma (HCC), the fifth most common cancer worldwide. The study conducted by Lubeiny et al. aimed to explore the effects of β-Sitosterol, a phytosterol with potential anticancer properties, on the biological processes of the hepatoma cell line HepG2 that had been transfected with the HBX gene. The researchers investigated how β-Sitosterol influences cell migration and gene expression, thereby contributing to the understanding of its potential as a therapeutic agent against HBV replication and cancer metastasis.
Impact of β-Sitosterol on Cell Viability
The research demonstrated that β-Sitosterol significantly affects the viability of HepG2.2.15 cells in a concentration-dependent manner. The cell viability assays revealed a decrease in the number of viable cells as the concentration of β-Sitosterol increased, suggesting its potential use in diminishing cell proliferation associated with HCC. The findings indicate that β-Sitosterol could play a role in inhibiting cell growth pathways, thereby offering insights into its utility as a treatment option for patients with HBV-induced liver malignancies.
Reduction in Cell Migration
Another key finding of the study was the substantial decrease in the migration of HepG2.2.15 cells upon treatment with β-Sitosterol. Using the Transwell migration assay, the researchers observed that the number of migrated cells treated with β-Sitosterol was significantly lower than in the control group. This reduction in migratory ability suggests that β-Sitosterol might be effective in minimizing metastasis, a common and severe complication of cancer. The relationship between cell migration and metastasis is crucial as it can lead to worse prognoses in patients with HCC.
Gene Expression Analysis
The study also explored the effects of β-Sitosterol on gene expression using gene chip analysis. The results showed significant alterations in the expression of genes associated with critical biological processes, particularly in the negative regulation of megakaryocyte and telomere maintenance. These findings underscore β-Sitosterol’s potential to modulate gene expression linked to HCC progression and its implications for target pathways involved in cancer biology and metastasis.
Conclusion
In summary, the research indicates that β-Sitosterol has notable effects on hepatocellular carcinoma by decreasing cell proliferation, reducing cell migration, and influencing gene expression. These findings highlight the compound’s potential as an anti-cancer agent, specifically in the context of HBV-related liver diseases. Further investigations into the specific mechanisms of β-Sitosterol, particularly its impact on telomere maintenance and histone methylation, could pave the way for developing new therapeutic strategies for managing HCC and improving patient outcomes in the context of chronic hepatitis B infection.
FAQ section (important questions/answers):
What is the aim of the study on β-Sitosterol?
The study aimed to evaluate the effects of β-Sitosterol on hepatitis B virus replication and metastasis in hepatoma cells, specifically in HepG2 transfected with the HBx gene.
How does β-Sitosterol affect cell migration in HepG2.2.15?
The results indicated that β-Sitosterol significantly decreases cell migration in HepG2.2.15 cells, suggesting a potential role in reducing metastasis associated with hepatocellular carcinoma.
What methods were used to assess the effect of β-Sitosterol?
The study utilized CCK8 assays for cell viability, Transwell assays for cell migration, and gene expression analysis through gene chips to evaluate the effects of β-Sitosterol.
What are the significant findings regarding gene expression?
The study found significant enrichment in the negative regulation of megakaryocytes and various biological processes that indicate potential pathways influenced by β-Sitosterol in hepatoma cells.
What role does HBx protein play in hepatocellular carcinoma?
The HBx protein is involved in oncogenesis and negatively regulates tumor suppressor genes, leading to chromosomal instability and contributing to the development of hepatocellular carcinoma.
What is the conclusion regarding β-Sitosterol's potential effects?
The study concludes that β-Sitosterol may reduce hepatocellular carcinoma metastasis and hepatitis B virus replication, suggesting its potential use as an anti-platelet agent.
Glossary definitions and references:
Scientific and Ayurvedic Glossary list for “Effect of b-sitosterol on HBx gene-transfected HepG2 cells.”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.
1) Cancer:
Cancer is a significant global health challenge, with hepatocellular carcinoma (HCC) being a major concern linked to chronic hepatitis B virus (HBV) infection. The study focuses on understanding the biological effects of β-Sitosterol on HCC to potentially mitigate cancer progression and improve therapeutic strategies.
2) Medium:
The term 'Medium' refers to the nutrient solutions used to culture cell lines, such as Dulbecco's Modified Eagle Medium (DMEM), which provides essential nutrients for cell growth and maintenance in laboratory experiments. It plays a critical role in studying cellular responses to treatments like β-Sitosterol.
3) Table:
In the context of research, 'Table' refers to a structured arrangement of data, often summarizing experimental conditions, results, or parameters. The tables mentioned provide details on plasmid preparation and experimental setups crucial for replicating the study findings and ensuring data clarity.
4) Beta:
β-Sitosterol, commonly referred to as Beta in this study, is a phytosterol that shows potential anti-cancer properties. The research investigates its effects on cell proliferation, migration, and the regulation of gene expression in hepatoma cells, aiming to explore its utility against HCC.
5) Viru:
Assuming 'Viru' relates to 'virus', it highlights the role of infectious agents like the hepatitis B virus (HBV) in promoting liver diseases, including hepatocellular carcinoma. The study focuses on understanding how β-Sitosterol might impair HBV replication and contribute to cancer treatment strategies.
6) Study (Studying):
The term 'Study' encompasses the systematic investigation undertaken to explore the biological effects of β-Sitosterol on the HepG2 cell line transfected with the HBx gene. It aims to contribute to understanding the relationship between HBV, HCC, and potential therapeutic agents.
7) Eagle:
Eagle refers to the name of the culture medium system developed by Harry Eagle, known as Eagle's Medium, which is foundational in cell culture practices. The use of such media in the study is essential for the growth and maintenance of liver cancer cell lines.
8) Cutan:
Sudan is the geographical location of Al-Neelain University, where the first author conducted part of the research. The country's endemic issues with hepatitis B underline the importance of addressing hepatitis-related cancers like HCC, thereby emphasizing the study's relevance to public health in the region.
9) Cotton:
Cotton is used in the laboratory for swabbing cell culture plates to remove non-migrated cells during migration assays. This technique ensures accurate measurement of cell migration, an important part of the experimental design aimed at assessing the effectiveness of β-Sitosterol.
10) Cina:
China is home to the Fujian Medical University, where part of the research was conducted. The collaboration highlights the growing importance of international partnerships in addressing global health issues like HBV and HCC, which are prevalent in various regions, including Asia.
11) Line:
In scientific research, 'Line' usually refers to a specific cell line used in experiments. The HepG2 cell line employed in the study is derived from human liver cancer cells and is pivotal for investigating the biological effects of potential treatments like β-Sitosterol.
12) Education:
In this context, Education refers to the academic and institutional framework that supports research and development in biomedical sciences. The study's affiliation with educational institutions indicates the role of research in advancing knowledge and addressing critical health challenges.
13) Medicine:
Medicine encompasses the field dedicated to diagnosing, treating, and preventing diseases. The research aims to explore new treatment avenues for HCC, emphasizing the importance of integrating research findings into clinical practices for better patient outcomes.
14) Disease:
The term 'Disease' signifies the pathological conditions being investigated in this study, particularly hepatitis B and hepatocellular carcinoma. Understanding these diseases' mechanisms and potential treatments is crucial for developing effective therapeutic strategies.
15) Fujian:
Fujian references the province in China where the Key Laboratory for Gastrointestinal Cancer is located. This laboratory plays a significant role in cancer research, underscoring the importance of regional efforts in tackling health issues like HCC and HBV.
16) Hela (Helá):
HeLa cells are an immortal cell line derived from cervical cancer cells, often used in scientific research. Mentioning HeLa in this context relates to previous studies on β-Sitosterol's effects on cell proliferation, providing a comparative background for the current research.
17) Discussion:
Discussion refers to the section of the research where findings are interpreted, contextualized, and implications are drawn. This part is essential for correlating experimental results with broader biological processes and other research in the field.
18) Quality:
Quality indicates the standard and reliability of the research data obtained, especially concerning RNA quantification and experimental methods. High-quality results ensure the validity of the conclusions drawn regarding the effects of β-Sitosterol on hepatoma cells.
19) Company:
Company in this context possibly refers to affiliated institutions or organizations that support the research, indicating the collaborative environment necessary for advancing scientific inquiries into cancer treatments. This highlights the synergistic effort in research endeavors.
20) Death:
Death signifies the ultimate outcome of many cancers, including hepatocellular carcinoma, which is linked to chronic hepatitis B. The study aims to investigate therapeutic methods that could reduce mortality associated with these diseases through effective treatments.
21) Zhen:
Zhen refers to one of the co-authors involved in the research, indicating the collaborative effort in conducting the study. The contributions from multiple researchers highlight the interdisciplinary nature of scientific research in addressing complex health issues like HCC.
Other Science Concepts:
Discover the significance of concepts within the article: ‘Effect of b-sitosterol on HBx gene-transfected HepG2 cells.’. Further sources in the context of Science might help you critically compare this page with similair documents:
Chronic hepatitis, Gene expression, Tumor suppressor gene, Biological effect, Cell viability assay, Hepatocellular Carcinoma, Cell migration, Plasmid construction.