Assessment of homocysteine and antioxidant enzymes levels in stroke

| Posted in: Science

Journal name: World Journal of Pharmaceutical Research
Original article title: Assessment of homocysteine and antioxidant enzymes levels in stroke
The WJPR includes peer-reviewed publications such as scientific research papers, reports, review articles, company news, thesis reports and case studies in areas of Biology, Pharmaceutical industries and Chemical technology while incorporating ancient fields of knowledge such combining Ayurveda with scientific data.
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Original source:

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Author:

Susan J. Ali, Wasen N. Hussain, Sura Z. Hussein and Ayoub A. Bazzaz


World Journal of Pharmaceutical Research:

(An ISO 9001:2015 Certified International Journal)

Full text available for: Assessment of homocysteine and antioxidant enzymes levels in stroke

Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research

Doi: 10.20959/wjpr20174-8155


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Summary of article contents:

Introduction

Stroke is a leading cause of disability and mortality worldwide, with increasing evidence linking elevated levels of total plasma homocysteine (tHcy) to this condition. Homocysteine, a non-protein α-amino acid resulting from the metabolism of methionine, has been suggested as a key risk factor for several cardiovascular diseases, including stroke. This study aimed to assess the levels of homocysteine, antioxidant enzymes, and lipid profiles in stroke patients compared to healthy individuals.

Role of Homocysteine in Stroke

Elevated homocysteine levels, or hyperhomocysteinemia, can lead to endothelial cell injury, inflammation in blood vessels, and ultimately atherogenesis, contributing to ischemic injury. Hyperhomocysteinemia can be classified into different severity levels based on plasma concentrations. Various factors—from dietary habits to hereditary abnormalities and lifestyle choices—can impact homocysteine levels. The study found that stroke patients exhibited significantly higher levels of tHcy compared to healthy controls, reinforcing the association between elevated homocysteine and stroke risk.

Oxidative Stress and Antioxidant Enzymes

Oxidative stress, characterized by an excess of reactive oxygen species (ROS) in the body, plays a pivotal role in the neuronal damage observed in ischemia-reperfusion injuries. In this study, plasma levels of superoxide dismutase (SOD) and malondialdehyde (MDA)—indicators of oxidation and oxidative stress—were measured alongside homocysteine. The results indicated a significant increase in SOD and MDA levels among stroke patients, suggesting a potential link between oxidative stress and the ischemic stroke mechanism.

Lipid Profiles in Stroke Patients

The study also evaluated lipid profiles in both stroke patients and the control group. Specifically, cholesterol, triglycerides, low-density lipoprotein (LDL), and very low-density lipoprotein (VLDL) levels were significantly elevated in stroke patients. Interestingly, high-density lipoprotein (HDL) levels were notably lower in stroke patients compared to controls. This dysregulation of lipid profiles in conjunction with increased oxidative stress markers further supports the notion that metabolic changes accompany stroke conditions.

Conclusion

The findings of this study suggest that elevated homocysteine levels and higher markers of oxidative stress may play significant roles in the development of stroke. The marked differences in tHcy, SOD, MDA, and lipid profiles between stroke patients and healthy individuals warrant the consideration of these factors as routine screening markers. Monitoring these biomarkers may facilitate early identification of individuals at risk for stroke and promote preventive strategies to safeguard against neurological damage.

FAQ section (important questions/answers):

What is the role of homocysteine in stroke development?

Homocysteine, a non-protein amino acid, can contribute to endothelial injury, inflammation, and atherogenesis, leading to ischemic injury and increased risk of stroke, particularly when present at elevated levels in the blood.

How was plasma homocysteine measured in the study?

Plasma total homocysteine was determined using High-Performance Liquid Chromatography (HPLC) with ultraviolet detection at 254 nm, following established methodology to ensure accurate measurement.

What antioxidant enzymes were evaluated in stroke patients?

The study assessed superoxide dismutase (SOD) levels as the antioxidant enzyme. SOD is crucial for mitigating oxidative stress and protecting against cellular damage during stroke.

What were the significant findings related to lipid profiles?

Stroke patients exhibited significantly elevated levels of total cholesterol, triglycerides, and low-density lipoprotein (LDL), with a notable decrease in high-density lipoprotein (HDL) when compared to healthy controls.

What methods were used for lipid profile analysis?

Lipid profile analysis, including measurements of cholesterol and triglycerides, was conducted using colorimetric methods, specifically employing a Biolabo kit designed for accurate biochemical assessments.

What conclusions were drawn regarding antioxidant enzymes and stroke?

The study concluded that increased levels of homocysteine, along with antioxidant enzymes like SOD and MDA, indicate the significant role of oxidative stress in stroke, suggesting these markers could aid in early diagnosis.

Glossary definitions and references:

Scientific and Ayurvedic Glossary list for “Assessment of homocysteine and antioxidant enzymes levels in stroke”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.

1) Blood:
Blood is a vital fluid that circulates in the body, transporting oxygen and nutrients to tissues while removing waste products. In the context of this study, blood samples were collected to analyze the levels of homocysteine and antioxidant enzymes in stroke patients, which plays a significant role in assessing cardiovascular health and disease risk.

2) Disease:
Disease refers to any condition that impairs the normal functioning of the body, often resulting in symptoms and reduced quality of life. In this article, the focus is on stroke as a disease, with an examination of its relationship to blood parameters such as homocysteine levels, which are linked to cardiovascular health.

3) Male:
Male denotes the gender of an individual. In this study, the sample population included both male and female stroke patients. Understanding the gender distribution is important in epidemiological studies, as men and women may present different risks and responses to conditions like stroke and may require tailored healthcare strategies.

4) Science (Scientific):
Science is the systematic study of the natural world through observation and experimentation. This research is rooted in scientific principles, employing biochemical testing methods to analyze plasma levels of homocysteine, antioxidant enzymes, and lipids to understand their relationship to stroke, contributing to biomedical knowledge and potential treatment pathways.

5) Study (Studying):
A study is a detailed investigation and analysis of a specific topic to generate knowledge. In this context, the study investigates the levels of homocysteine and antioxidant enzymes in stroke patients compared to healthy individuals, aiming to uncover potential biomarkers for stroke risk and inform clinical practice for prevention and treatment.

6) Education:
Education encompasses the process of acquiring knowledge and skills. The research aims to highlight the relationship between plasma biomarkers and stroke, contributing to medical education by informing healthcare professionals about risk factors and management of stroke, ultimately improving patient care and preventive strategies in clinical settings.

7) Teaching:
Teaching involves facilitating learning and imparting knowledge to others. Findings from studies like this one can be used as educational material for teaching healthcare providers about the importance of monitoring biomarkers such as homocysteine levels in patients, thus enhancing their understanding of stroke risk assessment and prevention strategies.

8) Species:
Species typically refers to a group of organisms capable of interbreeding. Though this term usually applies to biological classifications, in this context, it may encompass the diversity of individuals in the study sample (both male and female stroke patients), reflecting variability in health conditions and response to stroke-related risk factors.

9) Table:
A table organizes and presents data clearly and concisely. In the article, Table 1 summarizes the findings of the study, displaying the mean levels of homocysteine, antioxidant enzymes, and lipid profiles between stroke patients and control groups. Such tables aid in quickly interpreting research results and facilitating comparisons.

10) Inflammation:
Inflammation is a biological response to harmful stimuli, often resulting in tissue damage. The study discusses inflammation's role in vascular health, indicating that elevated homocysteine may contribute to endothelial dysfunction and inflammatory processes that exacerbate conditions leading to stroke, underscoring the disease's pathophysiology.

11) Calculation:
Calculation is the mathematical process of determining a numerical result from data. This study utilizes statistical methods, including Student T-tests and Duncan’s Multiple Range Test, to analyze the significance of differences between stroke patients and controls, providing robust, reliable conclusions about the relationship between homocysteine and stroke risk.

12) Discussion:
Discussion sections in research papers critically analyze results and contextualize findings within existing literature. The discussion here evaluates the implications of elevated homocysteine and antioxidant enzyme levels in stroke patients, exploring their potential as biomarkers and discussing relevant mechanisms linked to stroke pathogenesis, informing future research directions.

13) Surface:
Surface relates to the outer-most layer of an organism or structure. In a biochemical context, the term might refer to the surfaces of cells where interactions occur, such as the endothelial surface in blood vessels, which is affected by factors like homocysteine and could lead to stroke via endothelial dysfunction.

14) Cancer:
Cancer is a group of related diseases characterized by uncontrolled cell growth. While this study does not directly investigate cancer, it mentions hyperhomocysteinemia's association with various diseases, including some cancer types. Understanding how elevated homocysteine influences health can inform research on its link to other serious conditions.

15) Reason:
Reason signifies the underlying cause or explanation for an occurrence. In this context, the study explores reasons for increased levels of homocysteine and antioxidant enzymes in stroke patients, aiming to establish causative associations that could inform clinical assessments and preventive strategies against stroke.

16) Death:
Death refers to the cessation of all biological functions that sustain life. In the discussion of stroke, a leading cause of global mortality, understanding risk factors like plasma homocysteine levels provides insights into prevention strategies which could ultimately reduce death rates associated with traumatic health events like stroke.

17) Sura (Shura):
Sura likely refers to an author or contributor to the study, signaling the collaborative nature of research. Recognizing individual contributions helps in understanding the diverse expertise brought to the investigation, enhancing the study's credibility and ensuring a comprehensive approach to examining the topic under discussion.

18) Sah:
Shah, similar to Sura, likely denotes an author involved in the research and analysis presented in the study. Attributing work to specific individuals acknowledges their role in data collection, interpretation, or publication, which is essential in the academic context for accountability and advancement of scientific knowledge.

19) Meat:
Meat includes animal-derived food products, which are discussed in the context of homocysteine. The study indicates that high dietary intake of meat may elevate homocysteine levels, linking nutritional factors to stroke risk and highlighting the importance of dietary considerations in managing cardiovascular and neurological health.

Other Science Concepts:

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Discover the significance of concepts within the article: ‘Assessment of homocysteine and antioxidant enzymes levels in stroke’. Further sources in the context of Science might help you critically compare this page with similair documents:

Oxidative stress, Ischemic Stroke, Lipid profile, Cardiovascular disease, Lipid peroxidation, Stroke patients, Malondialdehyde (MDA), Superoxide dismutase (SOD), Free Radical, Antioxidant enzyme.

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