Phytochemical and antioxidant evaluation of Artemisia absinthium flowers

| Posted in: Science

Journal name: World Journal of Pharmaceutical Research
Original article title: Phytochemical studies and evaluation of antioxidant activity and cytotoxicity of artemisia absinthium flowers
The WJPR includes peer-reviewed publications such as scientific research papers, reports, review articles, company news, thesis reports and case studies in areas of Biology, Pharmaceutical industries and Chemical technology while incorporating ancient fields of knowledge such combining Ayurveda with scientific data.
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Original source:

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Author:

Enas Ali Hassan Alkangar, Mayada Mawyah Siddig, Manazil Elhaj Ibrahim, Ola Alfadel Abozeid, Olla Siddig Elawad Alshaikh and Tagwa Mohammed Sabah


World Journal of Pharmaceutical Research:

(An ISO 9001:2015 Certified International Journal)

Full text available for: Phytochemical studies and evaluation of antioxidant activity and cytotoxicity of artemisia absinthium flowers

Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research

Doi: 10.20959/wjpr20177-8880


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Summary of article contents:

Introduction

Artemisia absinthium Linn., commonly known as Wormwood, is a significant perennial shrub belonging to the Asteraceae family. This study focuses on analyzing the phytochemical composition, antioxidant activities, and cytotoxic effects of the flowers of Artemisia absinthium. Various extraction methods such as maceration and Soxhlet extraction utilizing different solvents (petroleum ether, chloroform, acetone, and 70% ethanol) were employed to ascertain the most effective means of extracting cytotoxic components. The extracted samples were subjected to phytochemical screening, thin-layer chromatography (TLC), and cytotoxicity assessment through a brine shrimp lethality bioassay.

Antioxidant Activity and Phytochemical Composition

The findings indicated a diverse presence of secondary metabolites within the extracts, including essential oils, saponins, flavonoids, tannins, alkaloids, cardiac glycosides, and anthraquinones. Notably, the antioxidant activity was evaluated using the DPPH method, revealing that the Soxhlet petroleum ether extract demonstrated low scavenging activity (RSA, 46%). The total flavonoid content in the extracts varied, with the acetone Soxhlet and ethanol maceration extracts showing values of 0.86 and 0.02 mg quercetin equivalent per mg of dry extract, respectively. Likewise, the tannin content was assessed, showing values of 1.0 mg tannic acid equivalent for acetone Soxhlet and 1.25 mg for ethanol maceration extracts.

Cytotoxicity Assessment and Lethality Results

A crucial aspect of this research involved evaluating the cytotoxic potential of the Artemisia absinthium extracts using the brine shrimp lethality bioassay, which is a reliable preliminary method for assessing plant toxicity. The results illustrated that the Soxhlet petroleum ether extract exhibited the highest lethality with a lethal concentration 50 (LC50) of 63.0 µg/ml, categorizing it as highly toxic. In comparison, other solvent extracts, particularly chloroform and acetone, revealed lower toxicity profiles, suggesting their potential as non-toxic or moderately toxic. These outcomes indicate the effectiveness of petroleum ether extraction in retrieving cytotoxic compounds from the plant’s flowers.

Extraction Methods and Their Impact

The study highlighted the significance of extraction methods on the yield and phytochemical profile of the extracts. It was observed that the Soxhlet extraction method, despite yielding lower percentages compared to maceration, resulted in extracts with significantly higher cytotoxicity levels. This suggests that exhaustive extraction processes can effectively concentrate bioactive compounds that may contribute to the cytotoxic effects observed. The presence of various compounds identified through gas chromatography further supports the potential therapeutic applications of Artemisia absinthium in traditional medicine, particularly in treating cancer-related ailments.

Conclusion

In conclusion, Artemisia absinthium demonstrates a rich profile of phytochemicals, particularly flavonoids and tannins, that exhibit notable cytotoxicity, especially when extracted using petroleum ether through the Soxhlet method. The study underscores the importance of proper extraction techniques in obtaining effective natural products for potential therapeutic use. Given the rising interest in natural antioxidants and anticancer agents, Artemisia absinthium presents a promising candidate for future research and application in traditional and modern medicine. The research also emphasizes the necessity for further studies to explore the mechanisms underlying the observed cytotoxicity, aiming to harness the therapeutic potential of this plant in combating various diseases.

FAQ section (important questions/answers):

What is the focus of the study on Artemisia absinthium flowers?

The study aimed to evaluate the cytotoxicity of Artemisia absinthium flowers using various extraction methods and solvents while determining which method yields the highest extraction capacity for cytotoxic components.

Which extraction methods were used to analyze Artemisia absinthium?

The study utilized two extraction methods: maceration and exhaustive Soxhlet extraction, employing solvents such as petroleum ether, chloroform, acetone, and 70% ethanol.

What were the significant findings about phytochemical content?

Phytochemical screening revealed the presence of essential oils, tannins, flavonoids, alkaloids, and saponins in the extracts, indicating a rich diversity of bioactive compounds.

How was the antioxidant activity of the extracts evaluated?

Antioxidant activity was assessed using the DPPH radical scavenging method, comparing the extracts' performance against a standard antioxidant, propyl galate.

What did the GC analysis reveal about the extracts?

Gas chromatography analysis identified about 18 compounds in the petroleum ether Soxhlet extract, primarily consisting of fatty acid esters.

What conclusions were drawn regarding the cytotoxicity of Artemisia absinthium?

The study concluded that Artemisia absinthium exhibits high cytotoxicity, particularly emphasized by the petroleum ether Soxhlet extract, demonstrating its potential in further medicinal applications.

Glossary definitions and references:

Scientific and Ayurvedic Glossary list for “Phytochemical and antioxidant evaluation of Artemisia absinthium flowers”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.

1) Flavonoid:
Flavonoids are a group of plant metabolites known for their antioxidant properties. They are significant in the study of medicinal plants, like Artemisia absinthium, which may contribute to cytotoxic effects against cancer cells. Their presence was notably high in acetone extracts, indicating potential therapeutic value.

2) Table:
Tables are essential in research papers for organizing and summarizing data clearly. In the study, tables present yield percentages, phytochemical screening results, and chromatographic analysis, providing a succinct overview of findings related to the efficacy and components of Artemisia absinthium extracts.

3) Water:
Water as a solvent plays a crucial role in extraction processes. Various methods employed in the study utilized water alongside organic solvents to isolate bioactive constituents from Artemisia absinthium. It can influence the extraction efficiency and the types of compounds retrieved.

4) Phytochemical:
Phytochemicals are biologically active compounds found in plants that possess various health benefits. The significant presence of phytochemicals such as flavonoids, tannins, and alkaloids in Artemisia absinthium contributes to its antioxidant and cytotoxic properties, which are critical in evaluating its potential therapeutic uses.

5) Disease:
Diseases like cancer, diabetes, and coronary heart disease can be exacerbated by oxidative stress. The research emphasizes the importance of natural products, such as those from Artemisia absinthium, in developing alternative therapies to combat these diseases, particularly when traditional options may have adverse effects or high costs.

6) Toxicity:
Toxicity refers to the degree to which a substance can harm organisms. The study assessed cytotoxicity via the brine shrimp lethality bioassay, determining the lethal concentration (LC50) values, which indicate the potential harmful effects of the extracts from Artemisia absinthium.

7) Activity:
Activity in this context refers to the biological effects of phytochemicals, such as antioxidant and cytotoxic activities. The study evaluated the effectiveness of extracts from Artemisia absinthium in exhibiting these activities, indicating the potential for use in therapeutic applications against various diseases.

8) Cutan:
Sudan is the geographical context of the study, where Artemisia absinthium was harvested. The climatic and environmental conditions of Sudan contribute significantly to the properties of the plants studied, including their phytochemical profiles, which may influence their medicinal effectiveness.

9) Medicine:
Medicine refers to the science or practice of diagnosing, treating, and preventing diseases. The study explores traditional medicinal uses of plants, highlighting how natural products like Artemisia absinthium may play integral roles in healthcare and enhancing therapeutic strategies in modern medicine.

10) Cancer:
Cancer is a significant focus of modern medical research due to its morbidity and mortality rates. The study investigates the cytotoxic properties of Artemisia absinthium extracts, looking for potential anticancer effects that could contribute to new treatment options, especially given the limitations of current therapies.

11) Medium:
In biological contexts, 'medium' often refers to the environment in which experiments are conducted. The study utilized various mediums, including different solvents for extraction and artificial seawater for brine shrimp assays, to evaluate the cytotoxicity and health effects of Artemisia absinthium.

12) Study (Studying):
The term study signifies the systematic investigation aimed at understanding the cytotoxicity and antioxidant properties of Artemisia absinthium. It employs various methodologies to evaluate the effects and potential medicinal applications of phytochemicals derived from this plant.

13) Salina (Shalina):
Salina refers to the brine shrimp, Artemia salina, used in toxicity assessments within the study. These organisms provide a convenient model for evaluating the cytotoxic effects of plant extracts, as their sensitivity to harmful substances offers insight into the safety and efficacy of those extracts.

14) Species:
Species in biology denotes the basic unit of classification, representing a specific group of organisms. The study focuses on Artemisia absinthium as a species, exploring its unique properties and potential uses in medicine, particularly regarding its phytochemical content and biological activities.

15) Family:
Family classifies a group of related plants within a larger taxonomic hierarchy. Artemisia absinthium belongs to the Asteraceae family, which comprises various plants recognized for their medicinal properties. The classification aids researchers in understanding botanical relationships and potential uses.

16) Field:
Field can refer to a subject area of study or research. In this context, it relates to the field of pharmacognosy and phytochemistry, focusing on examining natural products and their potential applications in pharmacology, particularly in developing new medicinal therapies.

17) Fever:
Fever is often a symptom of underlying infections or disease processes. In traditional medicine, herbs like Artemisia absinthium have been used to alleviate such symptoms. The study may evaluate the effectiveness of this herb in treating fevers or related conditions.

18) Drug:
Drugs are substances used for medical treatment. This study emphasizes the importance of exploring natural compounds from plants like Artemisia absinthium as potential sources for new drugs, particularly for diseases with challenging treatments, such as cancer.

19) Civilization:
Civilization refers to complex human societies that develop over time, notably in managing health and disease. Historical use of plants in natural medicine can influence contemporary practices, making understanding traditional medicinal uses imperative in the context of modern civilizations.

20) Chemotherapy:
Chemotherapy is a common but often harsh treatment for cancer. The study highlights the need for alternative medicines derived from plants like Artemisia absinthium, potentially reducing the side effects associated with standard chemotherapy while maintaining efficacy against cancer cells.

21) Inflammation:
Inflammation is an immune response that can indicate various underlying health issues. The study may explore how the phytochemicals in Artemisia absinthium possess anti-inflammatory properties, providing insights into their role in managing inflammation and related conditions.

22) Calculation:
Calculation is vital in experiments to determine concentrations, yields, and toxic dosages. The study employs various calculations to analyze data, set up experiments accurately, and derive meaningful conclusions regarding the effects of Artemisia absinthium extracts.

23) Observation:
Observation is crucial in research to gather data and assess biological effects. The study relies on careful observation of the results from phytochemical screenings, extraction yields, and cytotoxicity assays to draw conclusions about the efficacy of Artemisia absinthium.

24) Discussion:
Discussion refers to the section of a research paper where findings are interpreted and contextualized. This study's discussion assesses the implications of the results and their relevance to existing knowledge on medicinal plants, particularly concerning Artemisia absinthium.

25) Pesticide:
Pesticides are substances used to kill pests, and some natural products from plants can exhibit pesticide-like properties. The study may highlight the dual relevance of Artemisia absinthium in both medicinal and agricultural contexts, potentially influencing pest management strategies.

26) Swelling:
Swelling is often a symptom related to inflammation or injury. The study may explore traditional uses of Artemisia absinthium in addressing swelling, focusing on its potential anti-inflammatory properties and how these might translate into therapeutic benefits.

27) Debility:
Debility refers to physical weakness or incapacity. The study may highlight how Artemisia absinthium has been used as a tonic traditionally to help alleviate symptoms of debility, thereby emphasizing the herb's potential health benefits.

28) Gelatin:
Gelatin can be involved in various tests and assays. The study may reference gelatin in the context of methodologies used to assess phytochemical presence or effects, indicating its role in the formulation of experimental procedures.

29) Bitter:
Bitter is a flavor profile often associated with medicinal plants. Artemisia absinthium is noted for its bitter taste, which has historical significance in its use as a medicinal herb, as many bitter compounds are thought to possess therapeutic properties.

30) Nature:
Nature encompasses the plant's intrinsic properties and its interactions within ecosystems. The study examines the natural compounds present in Artemisia absinthium, linking their chemical structures with biological activities, illustrating the relationship between natural products and their medicinal use.

31) Indian:
Indian refers to the traditional use of plants in Indian medicine, such as Ayurvedic practices. The study's focus on Artemisia absinthium may connect with historical practices in Indian civilizations that utilized various plants for their health benefits, influencing modern research.

32) Food:
Food relates to the consumption of plants for nourishment, and some medicinal plants also serve culinary purposes. The study may explore the duality of Artemisia absinthium as both food and medicine, emphasizing its versatility in human health.

33) Wine:
Wine, particularly absinthe, is flavored with Artemisia absinthium. The study may consider the historical and cultural significance of using this herb in alcoholic beverages while evaluating its potential health benefits and risks associated with consumption.

34) Line:
Line can refer to establishing correlations between variables in research. The study employs line graphs to visualize relationships between concentrations of extracts and observed effects, assisting in understanding trends in the data collected.

35) Hand:
Hand may refer to the tactile engagement with nature and herbs in traditional medicine practices. Researchers may informally discuss traditional methods or share insights gained ‘by hand’ through direct experience with plants like Artemisia absinthium.

36) Worm:
Worm relates to the name Wormwood, derived from the use of Artemisia absinthium as an anthelmintic agent traditionally used to expel parasitic worms. This historical context aligns with the exploration of the plant's medicinal properties in the study.

37) Salt (Salty):
Salt, specifically in establishing artificial seawater for brine shrimp tests, plays a role in the study's methodology. Proper salt concentrations help maintain brine shrimp viability, ensuring accurate toxicity assessments of Artemisia absinthium extracts.

Other Science Concepts:

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Discover the significance of concepts within the article: ‘Phytochemical and antioxidant evaluation of Artemisia absinthium flowers’. Further sources in the context of Science might help you critically compare this page with similair documents:

Medicinal plant, Flavonoid, Essential oil, Antioxidant activity, Phytochemical screening, Thin Layer Chromatography, Oxidative stress, Secondary metabolite, Cancer therapy, Brine shrimp lethality bioassay, Maceration method, Total flavonoid content, Gas chromatography, Soxhlet extraction, Cytotoxicity, Total tannin content, Anthraquinones, Natural source.

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