Antibacterial and antioxidant activity of Salvadora persica extracts
Journal name: World Journal of Pharmaceutical Research
Original article title: Evaluation of antibacterial and antioxidant activity of ethanolic extracts of salvadora persica
The WJPR includes peer-reviewed publications such as scientific research papers, reports, review articles, company news, thesis reports and case studies in areas of Biology, Pharmaceutical industries and Chemical technology while incorporating ancient fields of knowledge such combining Ayurveda with scientific data.
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T. Mohammad Munawarand D. Muralidhara Rao
World Journal of Pharmaceutical Research:
(An ISO 9001:2015 Certified International Journal)
Full text available for: Evaluation of antibacterial and antioxidant activity of ethanolic extracts of salvadora persica
Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research
Doi: 10.20959/wjpr20178-9118
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Summary of article contents:
Introduction
The study focuses on the evaluation of the antibacterial and antioxidant activities of ethanolic extracts from the leaves and stems of Salvadora persica, commonly known as miswak. This medicinal plant is traditionally used for oral hygiene in various cultures, particularly in Asian and Middle Eastern countries. Despite extensive research on its effects on oral microorganisms, there is limited information on its efficacy against other human pathogens, especially multidrug-resistant (MDR) isolates. This research aims to assess the potential of Salvadora persica as a source of bioactive compounds that may exhibit antibacterial and antioxidant activities.
Antioxidant Activity of Salvadora persica
A key focus of the study was to determine the antioxidant properties of the ethanolic extracts. The antioxidant activity was evaluated using several assays, including DPPH, nitroblue tetrazolium (NBT), and ferric reducing power (FRAP) methods. The results indicated that the leaf extract demonstrated a stronger antioxidant activity compared to the stem extract. The DPPH scavenging abilities were notably higher for the leaves, suggesting the extracts are rich in phenolic and flavonoid compounds. This correlation highlights the potential of Salvadora persica leaves as a natural source of antioxidants that can neutralize free radicals and combat oxidative stress.
Antibacterial Activity Against Human Pathogens
The antibacterial effects of the ethanolic extracts were tested against both Gram-positive and Gram-negative bacteria. The study revealed that both the leaf and stem extracts exhibited significant antibacterial activity, with the stem extract showing a higher efficacy. The Minimum Inhibitory Concentration (MIC) values suggested that Salvadora persica is particularly effective against Pseudomonas aeruginosa and Bacillus subtilis. The differential antibacterial activity observed between the extracts may be attributed to the varying levels of bioactive compounds present, particularly flavonoids, which have been associated with inhibiting bacterial growth and metabolism.
Correlation Between Phytochemical Content and Biological Activity
The research established a positive relationship between the phenolic and flavonoid content of the extracts and their respective antioxidant and antibacterial activities. The total phenolic content was found to be significantly higher in leaf extracts than in stem extracts, mirroring the observed biological activities. This supports the notion that the pharmacological effectiveness of Salvadora persica is linked to its rich chemical composition, which plays an essential role in its ability to scavenge free radicals and inhibit microbial growth. These findings suggest that the assessment of total phenolic and flavonoid content can serve as a useful screening method for evaluating the antioxidant capacity of plant extracts.
Conclusion
The study concludes that Salvadora persica is a promising source of antioxidant and antibacterial agents, with its leaves exhibiting superior activity compared to the stems. The identified health benefits and biochemical properties of this plant underscore its potential application as a natural preservative and health supplement. Further research is warranted to isolate specific bioactive compounds responsible for these beneficial effects and to explore their pharmacological applications more broadly. Overall, this study adds valuable knowledge to the existing literature on medicinal plants and encourages utilization of Salvadora persica in both nutritional and pharmaceutical contexts.
FAQ section (important questions/answers):
What is the main focus of the research on Salvadora persica?
The study evaluates the antibacterial and antioxidant activity of ethanolic extracts from the leaves and stem of Salvadora persica against various human pathogens, especially multidrug-resistant isolates.
Which bacteria did the extracts of Salvadora persica inhibit?
The extracts demonstrated antimicrobial effects against both Gram-positive bacteria like Staphylococcus aureus and Bacillus subtilis, and Gram-negative bacteria including Escherichia coli and Pseudomonas aeruginosa.
How was the antioxidant activity of the extracts assessed?
Antioxidant activity was measured using various assays, including DPPH free radical scavenging, NBT, and ferric reducing power (FRAP), showing the leaves extract had stronger activity than the stem extract.
What were the total phenolic and flavonoid contents in the extracts?
The total phenolic content was 68 mg GAE/g for leaves and 42 mg GAE/g for stems. The flavonoid content was 38 mg RU/g for leaves and 21 mg RU/g for stems.
Why is Salvadora persica important in traditional medicine?
Known as miswak, Salvadora persica has been traditionally utilized for oral hygiene and possesses potential medicinal properties due to its high content of antioxidants and antibacterial compounds.
What are the potential applications of this research on Salvadora persica?
The findings suggest that extracts of Salvadora persica can be used as natural antioxidants and preservatives in various food and non-food systems, alongside further exploration of its bioactive compounds.
Glossary definitions and references:
Scientific and Ayurvedic Glossary list for “Antibacterial and antioxidant activity of Salvadora persica extracts”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.
1) Activity:
Activity refers to the ability of a substance, in this context, the ethanolic extracts from Salvadora persica, to exert antimicrobial and antioxidant effects. The study assesses both antibacterial and antioxidant activities, which are critical for evaluating the potential medicinal uses of the plant extracts against various pathogens and oxidative stress.
2) Antibiotic (Antibacterial):
Antibacterial indicates the capability of a substance to inhibit bacterial growth or kill bacteria. In the study, the ethanolic extracts of Salvadora persica demonstrated significant antibacterial activity against various Gram-positive and Gram-negative bacteria, including multidrug-resistant strains, highlighting the extracts' potential as natural antimicrobial agents.
3) Flavonoid:
Flavonoids are a class of plant secondary metabolites known for their antioxidant properties and health benefits. They contribute to the antioxidant activity observed in the study's extracts. High flavonoid content is associated with the inhibition of certain bacterial strains and may signify the plant's therapeutic potential in treating infections.
4) India:
India is the geographical context of the research, where Salvadora persica plants were collected for study. The diverse climate and rich biodiversity in India allow for numerous medicinal plants, contributing to traditional medicine. The findings from this research can have implications in local medicinal practices in rural India.
5) Table:
Table in the document refers to a visual representation of data that organizes findings such as total phenolic and flavonoid contents or minimum inhibitory concentrations (MIC) of the extracts. Tables facilitate easy comparison and comprehension of results, essential in scientific communication to summarize complex data.
6) Study (Studying):
Study signifies the systematic investigation conducted to evaluate the antibacterial and antioxidant activities of Salvadora persica extracts. It encompasses methodology, data collection, and analysis, thus contributing to the scientific body of knowledge on natural products and their potential applications in pharmacology and health.
7) Aureus:
Staphylococcus aureus, often abbreviated as aureus in the study, is a Gram-positive bacterium targeted for antibacterial evaluation. It is notorious for causing skin infections and is often resistant to multiple antibiotics, making it a significant pathogen in clinical settings and justifying the need for the study's investigation on natural alternatives.
8) Water:
Water acts as a universal solvent and is essential in the extraction process of bioactive compounds from Salvadora persica. The study utilizes water in various assays and methods, highlighting its role in biological activity assessment, as well as demonstrating the importance of proper solvent choice in pharmacognosy.
9) Food:
Food represents the broader category in which the extracts from Salvadora persica can be utilized, particularly as natural preservatives due to their antioxidant and antimicrobial properties. This study contributes to the discussion on functional foods and the potential of plant extracts in enhancing food safety and nutrition.
10) Medicine:
Medicine refers to the field concerned with the prevention, diagnosis, and treatment of disease. The findings from the study suggest that the ethanolic extracts from Salvadora persica can be integrated into modern medicinal practices, potentially acting as natural alternatives to synthetic drugs in treating infections.
11) Disease:
Disease describes any abnormal condition affecting the body, often caused by pathogens such as bacteria. The study aims to provide insights into reducing the incidence of bacterial infections through natural extracts, highlighting the importance of discovering new antibacterial agents from medicinal plants to combat infectious diseases.
12) Andhra (Amdhra):
Andhra Pradesh is the Indian state where the authors conducted their research. The geographical relevance underscores the ecological diversity that may influence the phytochemical profiles of Salvadora persica, emphasizing the significance of local flora in developing treatments through bioprospecting indigenous plants native to the region.
13) Powder:
Powder refers to the form of the plant material processed for extraction. In the study, the leaves and stems of Salvadora persica were ground into a fine powder to facilitate the extraction of bioactive compounds, enabling effective assessment of their antioxidant and antibacterial properties during the research.
14) Species:
Species designates the specific category of living organisms. In the study, Salvadora persica is identified as a distinct plant species with various potential medicinal properties. Understanding species-specific characteristics is critical for pharmacological research, as it influences the extraction and efficacy of bioactive compounds.
15) Hygiene (Hygienic):
Hygiene represents practices ensuring good health and preventing disease, particularly in oral health. The use of Salvadora persica traditionally for oral hygiene illustrates its cultural significance and potential therapeutic benefits, highlighting the historical relevance and contemporary implications of plant-based hygiene practices in preventing dental issues.
16) Botany:
Botany is the scientific study of plants. The identification and classification of Salvadora persica within the study highlight its importance in botany and its role in traditional medicine, as well as the exploration and documentation of phytochemicals that can be used for health benefits.
17) Tree:
Tree often indicates the growth form of Salvadora persica, which is a woody plant. The tree's physical characteristics may influence its bioactive composition and environmental adaptation strategies. Understanding tree species contributes to biodiversity conservation and the sustainable use of natural resources for medicinal purposes.
18) Rich (Rch):
Rich signifies the abundance or high content of specific compounds within the extracts, particularly in terms of phenolics and flavonoids. This richness correlates with greater antioxidant and antibacterial activities, suggesting that the plant holds significant medicinal potential, warranting further exploration in pharmacological contexts.
19) Drug:
Drug relates to any substance used for medical treatment or as a pharmacological agent. The study implies that extracts from Salvadora persica could serve as potential drug candidates due to their antimicrobial and antioxidant properties, adding to the development of plant-based medicines for various health applications.
20) Pharmacological:
Pharmacological relates to the branch of medicine concerned with the study of drug action and the properties of medicinal substances. The findings regarding the antibacterial and antioxidant activities of Salvadora persica lay foundation for future pharmacological studies aimed at deriving therapeutic agents from this plant.
21) Phytochemical:
Phytochemical refers to the bioactive chemical compounds derived from plants, like those found in Salvadora persica. The study focuses on isolating and evaluating these phytochemicals for their biological activities, reinforcing the significance of plant research in discovering new health-promoting substances from natural sources.
22) Discussion:
Discussion represents the section of the research where results are interpreted and contextualized within existing literature. Here, findings regarding the antibacterial and antioxidant properties of Salvadora persica are analyzed, assessed, and compared with previous studies to enhance scientific understanding and guide future research directions.
23) Science (Scientific):
Scientific denotes a systematic approach based on empirical evidence and experimentation. The study employs scientific methods to explore the properties of Salvadora persica, emphasizing the integrity and reliability of research in contributing to the body of knowledge in pharmacognosy and natural product chemistry.
24) Karnataka:
Karnataka is the Indian state from where the Salvadora persica samples were gathered. The geographical context is significant as it highlights regional biodiversity and the availability of different plant species, suggesting that localized studies can enhance the understanding of bioactive compounds unique to the local flora.
25) Tirupathi:
Tirupathi is a city in Andhra Pradesh near where certain reagents were sourced for the study. The mention of Tirupathi emphasizes the local context of scientific research, highlighting the importance of regional resources and collaborations that contribute to experimental procedures and research findings.
26) Substance:
Substance refers to a particular kind of matter with uniform properties, emphasizing the chemical composition in this study. The focus on natural substances like the ethanolic extracts of Salvadora persica showcases the importance of evaluating the raw materials derived from plants for their potential health benefits.
27) Muslin:
Muslin is a type of fine cotton cloth used for filtering the plant extracts in this study. Its use highlights the importance of proper laboratory techniques in isolating and preparing extracts, showcasing the procedural elements that contribute to the purity and effectiveness of the tested phytochemicals.
28) Family:
Family in this context refers to the families of plants, specifically Salvadoraceae, which includes Salvadora persica. Recognizing familial relationships among plant species is crucial for understanding their characteristics, potential uses, and evolutionary biology, laying a foundation for ethnobotanical and pharmacological investigations.
29) Cancer:
Cancer denotes a group of diseases characterized by abnormal cell growth. The study indirectly addresses cancer through the lens of antioxidants, as free radicals are linked to the development of cancer. The antioxidant properties of plant extracts may play a role in cancer prevention and health promotion.
30) Reason:
Reason pertains to the motivations behind conducting the study, primarily the quest for natural alternatives to synthetic substances. The increasing concern for antibiotic resistance and the quest for safe, effective treatments encourage the exploration of antioxidant and antibacterial properties in medicinal plants like Salvadora persica.
31) Medium:
Medium refers to the nutrient-rich environment used to culture bacteria in the antibacterial assays. Understanding the optimal growth conditions for bacterial strains is essential for accurate assessment of the antibacterial effects of the extracts, allowing for determination of their efficacy in inhibiting pathogenic growth.
32) Kadapa:
Kadapa is a city in Andhra Pradesh that serves as the location for the Department of Microbiology involved in antibacterial testing. The local context is relevant as it represents the geographical and educational backdrop for conducting research that contributes to regional knowledge and application of medicinal plants.
33) Ghat (Gham):
Ghat refers to the Western Ghats range in India, where the Salvadora persica plants were collected. This region is known for its rich biodiversity, making it a focal point for discovering various medicinal plants and their bioactive compounds. Geographic factors influence plant properties and distribution.
34) Hand:
Hand represents the method of manual processing in preparing plant materials. The phrase relates to traditional practices associated with preparing and using medicinal plants for health, emphasizing the link between historical knowledge and modern scientific exploration in understanding the medicinal utilization of natural resources.
Other Science Concepts:
Discover the significance of concepts within the article: ‘Antibacterial and antioxidant activity of Salvadora persica extracts’. Further sources in the context of Science might help you critically compare this page with similair documents:
Salvadora persica, Antibacterial activity, Antioxidant activity, Phytochemical analysis, Free radical scavenging activity, DPPH assay, Ethanolic extract, Total phenolic content, Biological properties, Gram positive bacteria, Gram negative bacteria, Total flavonoid content, Dose-dependent activity, Minimum inhibitory concentration (MIC), Plant material collection, Natural antioxidant.