Effects of UV radiation on Staphylococcus aureus and Klebsiella.
Journal name: World Journal of Pharmaceutical Research
Original article title: The effect of ultra violet radiation on bacteria (staphylococcus aureus and klebsiella pneumoniae)
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Ritu Kela (Ph.D), Nida Khan (M.Tech), Bilqueesa Mushtaq (B.sc), Mohd. Aziz (B.sc) and Neha Chaudhary (B.sc)
World Journal of Pharmaceutical Research:
(An ISO 9001:2015 Certified International Journal)
Full text available for: The effect of ultra violet radiation on bacteria (staphylococcus aureus and klebsiella pneumoniae)
Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research
Doi: 10.20959/wjpr20178-8969
Copyright (license): WJPR: All rights reserved
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Summary of article contents:
Introduction
The study presented in the World Journal of Pharmaceutical Research investigates the effects of ultraviolet (UV) radiation on two bacterial strains: Staphylococcus aureus and Klebsiella pneumoniae. This research emerges from growing concerns over microbial antibiotic resistance, a pressing issue in modern medicine. The objective of the study was to observe how UV exposure influences the bacteria's susceptibility to various antibiotics, ultimately contributing to the understanding of bacterial resistance mechanisms and the effectiveness of treatment options.
Impact of UV Radiation on Antibiotic Resistance
One key finding of the research is the effect of UV radiation on the efficacy of antibiotics against Staphylococcus aureus and Klebsiella pneumoniae. In the study, bacteria exposed to UV radiation for five hours demonstrated varied resistance levels to several antibiotics, including Cefixime, Oflaxacin, and Moxifloxacina. For instance, while Staphylococcus aureus showed a decrease in the zone of inhibition against most antibiotics, the effect of Moxifloxacina was notable for exhibiting an increased zone of inhibition post-UV exposure. This indicates that UV radiation can potentially alter the antibiotic responsiveness in certain bacterial strains.
Altered Efficacy and Survival Rates
Another important concept addressed in the study is the impact of UV radiation on the survival rates of the bacteria. The researchers monitored colony counts to assess bacterial survival after UV exposure. The findings demonstrated significant discrepancies in survival rates relative to antibiotic treatment, highlighting that UV exposure could either render bacteria more resilient or, conversely, make them more vulnerable to specific antibiotics. Such insights underline the complex interaction between UV radiation and bacterial survival, emphasizing the need for further research in this area.
Methodology and Antibiotic Sensitivity Testing
The study methodology involved the cultivation of isolated strains of Staphylococcus aureus and Klebsiella pneumoniae, followed by a comparative analysis of control (non-exposed) and test groups (UV-exposed). Antibiotic sensitivity was evaluated using the disk diffusion method, where various antibiotics were tested against bacteria post-UV treatment. This systematic approach not only aided in establishing baseline resistance profiles but also allowed for the assessment of how UV exposure could modify these profiles, providing valuable data for understanding therapeutic dynamics.
Conclusion
In conclusion, the study underscores the significance of understanding UV radiation's role in influencing bacterial resistance to antibiotics. By demonstrating that UV exposure can alter the efficacy of various antibiotics against Staphylococcus aureus and Klebsiella pneumoniae, the findings call for further investigation into this area. Such research is vital for developing more effective treatment strategies against drug-resistant bacterial infections and could inform clinical practices by integrating UV radiation as a potential therapeutic avenue. The implications of this study advocate for an ongoing dialogue surrounding antibiotic resistance and the exploration of novel approaches to combat it.
FAQ section (important questions/answers):
What was the main aim of this study on ultraviolet radiation?
The study aimed to understand the effect of ultraviolet radiation on bacteria, specifically Staphylococcus aureus and Klebsiella pneumoniae, and their responses to various antibiotics after UV exposure.
How were the bacterial strains isolated for this research?
The bacterial strains of Staphylococcus aureus and Klebsiella pneumoniae were isolated in a microbiology laboratory using basic microbiological techniques and confirmed through biochemical tests for purity.
What exposure duration was used for the bacteria to UV radiation?
The bacteria were exposed to ultraviolet radiation for a duration of 5 hours, while control samples were kept unexposed to evaluate the effects of UV radiation.
Which antibiotics were tested for sensitivity against UV-exposed bacteria?
The antibiotics tested for sensitivity include Cefixime, Oflaxacin, Ornidazole, Amoxicillin, Moxifloxacina, and Ciprofloxacin to evaluate the efficacy post UV exposure.
What were the findings related to antibiotic efficacy after UV exposure?
The results showed that UV-exposed Staphylococcus aureus had decreased zones of inhibition against most antibiotics, while Klebsiella pneumoniae showed mixed responses, indicating altered antibiotic efficacy.
What future research directions do the authors suggest?
The authors suggest further studies are needed to investigate the role of UV radiation in altering bacterial strains and their responsiveness to antibiotics, emphasizing the varying effects on different organisms.
Glossary definitions and references:
Scientific and Ayurvedic Glossary list for “Effects of UV radiation on Staphylococcus aureus and Klebsiella.”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.
1) Antibiotic (Antibacterial):
Antibiotics are substances that inhibit the growth of or destroy bacteria. They are crucial in treating bacterial infections, especially in the face of increasing antibiotic resistance. The study examined how ultra violet radiation affected the efficacy of various antibiotics against specific bacteria strains, highlighting the importance of understanding resistance mechanisms.
2) Aureus:
Aureus refers to 'Staphylococcus aureus', a common bacterium that can cause serious infections in humans. In this study, the effects of ultra violet radiation on 'Staphylococcus aureus' were investigated, revealing significant alterations in the bacteria's response to various antibiotic treatments, which is critical for antibiotic resistance research.
3) Education:
Education in the context of this study pertains to the training in biological and health sciences. The research was conducted in an academic institution in India, showcasing the role of higher education in advancing knowledge about microbiology, antibiotic resistance, and the effects of ultraviolet radiation on bacteria.
4) Science (Scientific):
Science is the systematic study of the structure and behavior of the physical and natural world. This research falls within the realm of microbiology and pharmacology, focusing on understanding the interactions between ultraviolet radiation and bacterial strains, and how these interactions affect antibiotic efficacy and resistance.
5) India:
India serves as the geographical context of this study, emphasizing the local relevance of antibiotic resistance. The research conducted at a college in Meerut, India, contributes to the global understanding of microbial resistance, helping to address pressing health challenges faced in Indian healthcare settings.
6) Table:
In scientific research, tables are used to present data clearly and concisely. The study included tables summarizing the results of antibiotic efficacy measurements against UV-exposed bacteria, facilitating comparison and analysis of how ultraviolet radiation affects bacterial response to various antibiotic treatments.
7) Study (Studying):
The term study refers to this specific research aimed at examining the impact of ultraviolet radiation on two types of bacteria. It highlights the need to investigate bacterial responses to environmental factors, thereby contributing valuable insights into microbial behavior and antibiotic efficacy, which is pertinent to public health.
8) Discussion:
In scientific literature, the discussion section interprets the research findings, contextualizing them within broader fields of study. This paper's discussion points to the implications of altered antibiotic efficacy due to UV exposure, suggesting directions for future research on microbial resistance and treatment strategies.
9) Drug:
Drugs are substances used to diagnose, cure, treat, or prevent diseases. In this study, various antibiotic drugs were tested against the UV-exposed bacteria. Understanding how these drugs interact with altered bacterial strains is essential for developing effective treatment regimens in light of antibiotic resistance.
10) Observation:
Observation refers to the act of monitoring or noting phenomena for scientific inquiry. The results of this study are based on careful observation of bacterial behavior pre- and post-ultraviolet exposure, providing empirical data necessary for understanding the effects of radiation on bacterial viability and antibiotic susceptibility.
11) Zoology:
Zoology is the branch of biology that studies animals. Although not directly the focus of this research, the mention of zoology highlights the broader implications of understanding microbial interactions and antibiotic resistance, which can relate to animal health and the impacts of radiative treatments on wildlife.
12) Wisdom:
Wisdom in this context refers to insights gained from experienced individuals in the field of microbiology and pharmacology. The researchers expressed gratitude towards knowledgeable mentors, underscoring the value of guided inquiry and collaboration in advancing the understanding of complex scientific issues like antibiotic resistance.
13) Repair:
Repair relates to the biological mechanisms that organisms use to mend damage, such as DNA repair in response to ultraviolet radiation. This study discusses how certain bacteria can potentially recover from UV-induced damage, which is crucial for understanding bacterial resilience and developing effective treatment strategies.
14) Medium:
Medium in microbiology refers to the nutrient-rich environments used to culture bacteria. The use of standardized culture media in the study ensures that bacterial growth can be consistently evaluated when testing the effects of ultraviolet radiation and antibiotic responses, emphasizing methodological rigor.
15) Animal:
Animal references in the study touch upon the implications that research on bacteria and antibiotic resistance can have on animal health. Understanding microbial responses and interactions is vital for veterinary medicine and can influence treatments for infections in both human and animal populations.
16) Pearl:
Pearl metaphorically refers to valuable insights or knowledge shared among researchers. The acknowledgment of 'sharing pearls of wisdom' suggests that collaboration and mentorship enriched the research process, emphasizing the importance of collective expertise in yielding significant findings in scientific studies.
17) Ritu (Rtu):
Ritu is the name of one of the researchers involved in the study. The inclusion of personal names highlights the individual contributions to the research and helps attribute credit to the efforts and expertise of the team members engaged in investigating the effects of ultraviolet radiation on bacteria.
18) Kela:
Kela is likely a surname of one of the lead researchers in this study, indicating their primary role within the research team. The authorship credits reinforce the importance of individual contributions in collaborative scientific work, fostering a well-rounded analysis of the effects of ultraviolet radiation on bacterial resistance.
19) Dish (Dis):
Dish refers to the laboratory containers used for culturing bacteria in this study. The careful use of dishes ensures controlled conditions for the bacterial growth experiments, showcasing the importance of precise methodologies in scientific research related to microbiology and antibiotic efficacy assessments.
Other Science Concepts:
Discover the significance of concepts within the article: ‘Effects of UV radiation on Staphylococcus aureus and Klebsiella.’. Further sources in the context of Science might help you critically compare this page with similair documents:
Zone of inhibition, Staphylococcus aureus, Nutrient agar, Antibiotic resistance, Multidrug resistant bacteria, Klebsiella pneumoniae, Ciprofloxacin, Biochemical techniques, Antibiotic sensitivity test, Ornidazole, Cefixime, Amoxicillin, Aerobic incubation, Ultra violet radiation, Anti microbial susceptibility testing, Recombinant bacteria, Oflaxacin, Moxifloxacina, Colony count, Test and control.