Hepatoprotective effects of Setaria megaphylla on paracetamol injury.

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Journal name: World Journal of Pharmaceutical Research
Original article title: Evaluation of hepatoprotective effects of setaria megaphylla (steud) t. dur and sphinz (poaceae) root extract on paracetamol-induced injury in rats.
The WJPR includes peer-reviewed publications such as scientific research papers, reports, review articles, company news, thesis reports and case studies in areas of Biology, Pharmaceutical industries and Chemical technology while incorporating ancient fields of knowledge such combining Ayurveda with scientific data.
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Original source:

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Author:

John Akpan Udobang and Jude Efiom Okokon


World Journal of Pharmaceutical Research:

(An ISO 9001:2015 Certified International Journal)

Full text available for: Evaluation of hepatoprotective effects of setaria megaphylla (steud) t. dur and sphinz (poaceae) root extract on paracetamol-induced injury in rats.

Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research

Doi: 10.20959/wjpr20179-9311

Copyright (license): WJPR: All rights reserved


Download the PDF file of the original publication


Summary of article contents:

Introduction

The study investigates the hepatoprotective effects of Setaria megaphylla (Steud) T. Dur and Schinz, a medicinal plant traditionally used by the Niger Delta community in Nigeria to address various health issues, including liver ailments. This research aims to evaluate the plant's potential protective qualities against liver damage caused by paracetamol in rats, particularly concerning biochemical and histological changes. The significance of these findings lies in the need to confirm the safety and efficacy of locally-used medicinal plants in managing liver health.

Hepatotoxicity Induced by Paracetamol

Paracetamol (acetaminophen) is widely recognized for its analgesic and antipyretic properties; however, its hepatotoxicity is a significant concern when taken in high doses. The research found that administering paracetamol to rats resulted in elevated liver enzymes (AST, ALT, ALP) and increased bilirubin levels, indicating liver damage. This damage occurred as paracetamol was metabolized to a toxic compound, N-acetyl-p-benzoquinone imine (NAPQI), which overwhelms the liver's detoxification capacity. The study demonstrates that the elevation of these markers serves as an effective model for evaluating liver toxicity.

Biochemical Effects of Setaria megaphylla Extract

The administration of Setaria megaphylla ethanol root extract significantly altered the biochemical parameters in the treated rats. The extract dosage ranging from 150 to 450 mg/kg led to a notable reduction in liver enzyme levels and bilirubin concentrations when compared to the paracetamol group. In contrast, the levels of total protein and albumin increased significantly, suggesting a protective effect on liver function. These biochemical changes support the hypothesis that the extract plays a crucial role in mitigating the harmful effects instigated by paracetamol toxicity.

Histopathological Observations

Histopathological evaluation of liver tissues revealed stark contrasts between the treated groups and those induced with paracetamol. The paracetamol-only group exhibited focal necrosis and significant inflammation, indicative of severe liver injury. In contrast, the liver tissues from groups treated with the Setaria megaphylla extract and the standard drug silymarin showed markedly less damage, with congestion and mild inflammation but no substantial necrosis or fatty changes. These findings suggest that the extract provides a protective effect against hepatotoxicity, aligning with the observed biochemical results.

Conclusion

The research provides substantial evidence that the ethanol root extract of Setaria megaphylla possesses significant hepatoprotective properties against paracetamol-induced liver damage in rats. The biochemical and histopathological findings confirm the traditional medicinal uses of the plant for liver-related ailments. Future studies are recommended to isolate and identify the active constituents of Setaria megaphylla and elucidate their mechanisms of action, which can lead to the establishment of standardized treatments based on this plant for liver health. This work highlights the importance of ethnobotanical research in promoting the safe use of local medicinal plants.

FAQ section (important questions/answers):

What is the focus of the study conducted by Udobang et al.?

The study evaluates the hepatoprotective effects of Setaria megaphylla root extract on paracetamol-induced liver injury in rats, investigating its potential protective properties.

How was the effectiveness of the extract measured in the study?

The effectiveness was assessed by measuring biochemical parameters like liver enzymes and bilirubin levels, along with histological examination of liver tissue after paracetamol administration.

What dosage levels of Setaria megaphylla extract were tested?

Dosages tested included 150, 300, and 450 mg/kg of the ethanol root extract, administered daily for eight days prior to paracetamol exposure.

What were the main findings regarding liver function from this study?

The extract significantly reduced liver enzyme levels and bilirubin, indicating hepatoprotection, while enhancing total protein and albumin levels compared to the paracetamol group.

What does the histological analysis reveal about liver tissue effects?

Histological analysis showed congestion and mild inflammation in the extract-treated groups, with no necrosis, indicating hepatoprotective effects against paracetamol toxicity.

What compounds in Setaria megaphylla may contribute to its liver protection?

Compounds such as flavonoids and alkaloids may provide antioxidant and free radical scavenging activities, contributing to the observed hepatoprotective effects of the extract.

Glossary definitions and references:

Scientific and Ayurvedic Glossary list for “Hepatoprotective effects of Setaria megaphylla on paracetamol injury.”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.

1) Inflammation:
Inflammation is a biological response to harmful stimuli, such as pathogens or damaged cells, characterized by redness, heat, swelling, and pain. This process plays a crucial role in the body's defense mechanisms and healing, but chronic inflammation can contribute to diseases like cancer and autoimmune disorders, making its study vital in pharmacology and toxicology.

2) Animal:
In research, 'animal' refers to non-human species used for experimentation to understand biological processes and test the effects of drugs or treatments. Animal models are essential for preclinical studies, as they provide insights into physiological and pathological conditions, helping in the development of therapeutics before human trials.

3) Water:
Water is a vital solvent and medium for biochemical reactions in living organisms. It is essential for life, participating in transportation of nutrients, waste elimination, and thermoregulation. In pharmacological studies, water is often used as a control solvent to ensure the efficacy of drug formulations during experiments.

4) Activity:
Activity refers to the observable effects or actions of substances in biological systems. In pharmacology, it describes how compounds exert effects on cells or organisms, which can include therapeutic benefits or adverse effects. Assessing the activity of medicinal plant extracts is crucial in evaluating their potential uses in treatment.

5) Table:
In scientific literature, tables summarize data efficiently, providing a clear comparison of results from experiments. They organize numerical data and findings, making it easier for researchers to interpret and analyze the significance of the results, such as changes in biochemical parameters due to treatment in pharmacological studies.

6) Blood:
Blood is a vital fluid that transports oxygen, nutrients, hormones, and waste products throughout the body. In toxicology and pharmacology, blood analysis is crucial for measuring the concentration of drugs, toxins, and biological markers, providing essential information about the effects of substances and the physiological state of organisms.

7) Pharmacology:
Pharmacology is the study of drugs, their mechanisms of action, therapeutic effects, and potential adverse effects. This discipline combines elements of medicine, chemistry, and biology to understand how drugs interact with biological systems. It is fundamental for developing new medications and therapies to treat various diseases.

8) Drug:
A drug is a chemical substance used to diagnose, cure, treat, or prevent diseases. In pharmacology, drugs are studied for their efficacy, safety, and mechanisms of action on biological systems. Understanding drug properties is essential for developing effective therapeutic agents and minimizing adverse effects.

9) Toxicity:
Toxicity refers to the degree to which a substance can cause harm to biological systems. It encompasses the effects of drugs, chemicals, and environmental factors on health. Assessing toxicity is crucial in pharmacology and toxicology to evaluate the safety of new compounds and the risks associated with exposure.

10) Science (Scientific):
Science is the systematic study of the structure and behavior of the physical and natural world through observation and experimentation. In the context of pharmacology and toxicology, scientific methods are employed to explore the effects of compounds, contributing to the understanding of drug actions and human health.

11) Toxicology:
Toxicology is the scientific study of the adverse effects of chemical substances on living organisms. It involves understanding the mechanisms of toxicity, identifying hazardous substances, and assessing risks. This field is critical in evaluating chemical safety, including drugs, environmental pollutants, and natural toxins.

12) Teaching:
Teaching in a scientific context refers to the process of imparting knowledge about subjects such as pharmacology, toxicology, and medicine. Educators play a vital role in training future researchers and healthcare professionals, enhancing understanding of drug actions, safety, and ethical practices in research and clinical settings.

13) Potter:
In the context of pharmacological research, Potter is likely a reference to a researcher or scientist involved in studies that elucidate drug metabolism and toxicity. Recognizing contributions from individuals such as Potter helps contextualize findings and advances in understanding pharmacological mechanisms and their implications for health.

14) Forest:
Forest refers to a specific ecosystem characterized by a high density of trees and plant species. In pharmacological research, natural habitats like forests are significant for biodiversity studies and the discovery of new medicinal plants, such as Setaria megaphylla, highlighting the relationship between nature and traditional healing practices.

15) Kumar:
Kumar is often a reference to a researcher contributing to the understanding of pharmacological effects and mechanisms of compounds. Recognizing contributors like Kumar is important for contextualizing scientific knowledge, as their work may involve studying phytochemicals and their health benefits in various biological systems.

16) House:
House, particularly in a biological context, often refers to an animal house or facility where animals are housed during experiments. Such facilities are critical for maintaining controlled conditions in research studies and ensuring the well-being of animal subjects during pharmacological and toxicological investigations.

17) Study (Studying):
Study refers to the systematic investigation or research of a particular subject area. In pharmacology and toxicology, studies are designed to evaluate the effects of substances on biological systems, contributing to the understanding of drug actions, safety, and therapeutic potentials in clinical applications.

18) Pain:
Pain is a complex sensory and emotional experience often signaling injury or illness. Understanding pain mechanisms is essential in pharmacology for developing analgesics and pain management strategies. Research into pain relief contributes significantly to improving the quality of life for individuals suffering from various health conditions.

19) Phytochemical:
Phytochemicals are bioactive compounds derived from plants that may have health-promoting properties. In pharmacology, studying phytochemicals is critical for discovering new drugs and understanding the health benefits of medicinal plants. Their antioxidant, anti-inflammatory, and hepatoprotective properties can contribute to developing natural therapies.

20) Alcoholism:
Alcoholism is a chronic disease characterized by an inability to control alcohol consumption, leading to significant health issues. In pharmacology, understanding alcoholism is essential for developing treatments and interventions that address its physiological and psychological effects, enabling better management and support for affected individuals.

21) Substance:
Substance refers to any material or compound studied in scientific research. In pharmacology and toxicology, substances are analyzed for their chemical properties, effects on biological systems, and therapeutic potential. This classification is essential for understanding the safety and efficacy of drugs and other chemicals.

22) Murugan (Murukan):
Murugan may reference a researcher involved in pharmacological studies, particularly focusing on natural compounds and their effects. Recognizing contributions from scientists like Murugan helps contextualize advancements in understanding the therapeutic applications of plant-derived substances and their mechanisms of action.

23) Samtosha (Samtosa, Santosa, Santosha, Santosh):
Santosa likely refers to a researcher whose work contributes to the body of knowledge in pharmacology or toxicology. Acknowledging scientists like Santosa is important to contextualizing research findings, especially those related to the effects of various compounds on biological systems.

24) Vijaya:
Vijaya typically denotes a researcher in the field of pharmacology or science, focusing on the exploration of medicinal properties. Citing individuals like Vijaya emphasizes collaborative efforts in scientific research to unravel the therapeutic potentials of plant-based substances and their impacts on health.

25) Thomas:
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26) Edema (Oedema):
Oedema is the medical term for swelling caused by excess fluid accumulation in tissues. Understanding oedema is crucial in pharmacology, as many medical conditions involve fluid retention. Research into treatments for oedema contributes to developing therapeutic strategies that mitigate this condition in affected patients.

27) Sharman (Sarma, Sarman, Sharma):
Sharma is likely a reference to a researcher contributing to pharmacological or toxicological studies. Recognizing contributions from researchers such as Sharma is essential for contextualizing the impacts of their work on systemic understanding and refinement of medical science, especially in drug discovery and use.

28) Botany:
Botany is the scientific study of plants, their physiology, structure, genetics, and ecology. In pharmacology, botany plays a crucial role in identifying medicinal plants, understanding their effects, and exploring phytochemicals that can be developed into drugs or therapeutic agents for treating various ailments.

29) Ghanta (Ghamta):
Ghanta likely references a researcher involved in pharmacology or pharmacognosy, studying the therapeutic effects of natural compounds. Recognizing contributions from scientists like Ghanta helps enrich the scientific context surrounding medicinal plants and their utilization in developing new treatments.

30) Mason:
Mason may refer to a researcher whose contributions to pharmacology or toxicology are notable. Acknowledging individuals like Mason is important to frame scientific discussions related to the development and assessment of drugs and their impacts on health and safety.

31) Fever:
Fever is an elevation in body temperature often indicative of infection or illness. Understanding fever is important in pharmacology for developing antipyretic medications and managing conditions associated with increased body temperature. Research contributes to improving treatment strategies and optimizing patient care.

32) Death:
Death signifies the end of life, a critical concept in pharmacology and toxicology that considers how substances can impact longevity and mortality. Understanding the mechanisms behind toxicity and harmful effects of drugs is essential in developing safer therapeutic options to prevent undesirable outcomes.

33) Jacob:
Jacob likely refers to a researcher in the field of pharmacology or toxicology whose studies contribute to our understanding of chemical interactions within biological systems. Recognizing such contributions is essential for framing current scientific discourse and acknowledging collaborative efforts in research.

34) Raja:
Raja may denote a researcher whose contributions to pharmacological studies focus on natural substances and their effects. Acknowledging individuals like Raja is crucial for contextualizing research outputs and emphasizing the collective advancement of scientific understanding in drug development and therapeutic effectiveness.

35) Salt (Salty):
Salt, often used as a seasoning and preservative, can also serve medicinal purposes. In the context of pharmacology, studying the effects of salt can help understand its role in fluid balance, hypertension, and related health conditions. Its properties are relevant for developing dietary recommendations and therapeutic interventions.

Other Science Concepts:

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Discover the significance of concepts within the article: ‘Hepatoprotective effects of Setaria megaphylla on paracetamol injury.’. Further sources in the context of Science might help you critically compare this page with similair documents:

Medicinal plant, Free radical scavenging activity, Animal Ethics Committee, Hepatoprotective effect, Mechanism of action, Significant increase, Standard drug Silymarin, Total bilirubin, Ethnomedicinal use, Biochemical parameter, Toxicity evaluation, Phytochemical constituent.

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