Callus induction from different explants in vigna unguculata l
Journal name: World Journal of Pharmaceutical Research
Original article title: Callus induction from different explants in vigna unguculata l
The WJPR includes peer-reviewed publications such as scientific research papers, reports, review articles, company news, thesis reports and case studies in areas of Biology, Pharmaceutical industries and Chemical technology while incorporating ancient fields of knowledge such combining Ayurveda with scientific data.
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A. Srujana and J. Naga Lakshmi
World Journal of Pharmaceutical Research:
(An ISO 9001:2015 Certified International Journal)
Full text available for: Callus induction from different explants in vigna unguculata l
Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research
Doi: 10.20959/wjpr201717-10391
Copyright (license): WJPR: All rights reserved
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Summary of article contents:
Introduction
Cowpea (Vigna unguiculata) is a significant tropical legume cultivated globally, especially in regions of West Africa. Known for its nutritional value, cowpea is a crucial source of proteins, vitamins, and minerals, and plays a vital role in agricultural economies as fodder and as a source of income for farmers. The plant's adaptability to varying soil and climatic conditions allows it to thrive in environments with low rainfall and poor nutrition, earning it the nickname "hungry-season crop." However, cowpea faces challenges in tissue culture, primarily due to its recalcitrance in in-vitro regeneration, making research on callus induction from different explants essential.
Profuse Callus Induction
The research presented explores the callus induction capabilities of cowpea's leaf and nodal explants in a Murashige and Skoog (MS) medium supplemented with various concentrations of plant hormones—particularly 2,4-Dichlorophenoxyacetic acid (2,4-D) and Kinetin (KN). Of particular note, the highest callus induction frequency was achieved using a combination of 3.5 mg/l of 2,4-D and 0.5 mg/l of KN, yielding an impressive 83.11% induction rate. This combination prompted explants to form profuse, friable callus within two to three weeks, indicating a strong response when exogenous hormone supply coincided with endogenous hormonal coordination.
Effects of Hormone Concentrations
Callus initiation largely depended on the hormonal concentrations applied to the culture medium. Through systematic experimentation, it was determined that varying the concentrations of 2,4-D and KN led to different rates of callus induction and growth. Specifically, when the concentrations were adjusted from 1.0 mg/l to 4.5 mg/l for 2,4-D, there was a noted increase in callus induction percentages and biomass. This consistent pattern underscores the importance of hormone balance in plant tissue culture, leading to more vital and productive explants.
Nutritional and Phytochemical Outcomes
The study also observed the nutritional implications of callus induction in cowpea. Following analysis, callus from leaf explants presented higher levels of phenolic content and flavonoids compared to leafy extracts from seedlings. This increase in phytochemicals may indicate potential for enhanced nutritional properties in in-vitro cultures. Comparisons suggest that well-managed callus induction conditions can yield biomass with improved health benefits, making a compelling case for utilizing callus cultures for future agricultural and nutritional applications.
Conclusion
In conclusion, the research effectively demonstrates that cowpea explants, particularly when exposed to optimal hormone concentrations, can produce substantial amounts of callus tissue, providing a foundation for further biotechnological applications. The findings not only have implications for improving regeneration techniques in cowpea but also highlight the need for specific hormonal balances for enhanced growth and nutritional profiles. Future studies could expand on these results to explore more diverse conditions or to investigate potential commercial applications of enhanced cowpea varieties derived from tissue culture methodologies.
FAQ section (important questions/answers):
What is Vigna unguiculata and its significance?
Vigna unguiculata, or cowpea, is a crucial legume crop known for its high protein quality. It thrives in low rainfall and poor soils, providing nutrition for humans and livestock, especially during dry seasons in West Africa.
What factors influence callus induction in cowpea explants?
Callus induction depends on the combination of auxins and cytokinins. In this study, the best results were observed with 2, 4-D and kinetin, specifically at concentrations of 3.5 mg/l and 0.5 mg/l, respectively.
What methods are used for plant tissue culture in the study?
In the study, leaf and nodal explants of cowpea were surface sterilized and cultured on MS medium supplemented with various hormones. Controlled conditions of temperature, light, and humidity were maintained for optimal callus growth.
What were the main findings regarding callus formation?
The study found that the optimal hormone combination of 2, 4-D and kinetin resulted in maximum callus induction of 83.11%. Both leaf and nodal explants produced similar results in terms of callus growth.
What is the duration for callus formation in this research?
Callus from the explants typically formed within two to three weeks. The callus culture was continuously maintained by transferring to fresh media every two weeks based on the growth rate.
How does the callus composition relate to phytochemical content?
The callus produced in the study exhibited increased phenolic and flavonoid content compared to in-vitro leaf extracts. This suggests a potential for enhanced health benefits in phytochemical analysis of callus tissue from cowpea.
Glossary definitions and references:
Scientific and Ayurvedic Glossary list for “Callus induction from different explants in vigna unguculata l”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.
1) Medium:
In plant tissue culture, the 'medium' refers to the nutrient-rich mix that supports the growth and development of plant cells, tissues, or organs. It contains essential elements like vitamins, minerals, and growth regulators that are critical for inducing callus formation and stimulating cell division, providing a conducive environment for in vitro studies.
2) Crop:
A 'crop' refers to a plant grown for agricultural purposes. In this study, cowpea (Vigna unguiculata) is highlighted as a vital crop due to its nutritional benefits and adaptability in various climatic conditions. Understanding crop biology can aid in improving yield and crop resilience, essential for food security.
3) Phytochemical:
Phytochemicals are bioactive compounds found in plants that may have health-promoting properties. The study examines the presence of these compounds in callus tissues compared to seedling extracts, showcasing how in vitro cultivation can enhance the accumulation of beneficial phytochemicals, vital for both human nutrition and medicinal uses.
4) Species:
A 'species' is a group of organisms that can interbreed and produce fertile offspring. In the context of the study, cowpea is part of the genus Vigna, which includes around 80 different species. Exploring species diversity is crucial for agriculture, as it influences crop resilience and productivity.
5) Water:
Water is essential for all living organisms, including plants. In tissue culture, water is used in the nutrient medium to provide hydration for plant cells. It plays a crucial role in various physiological processes, including nutrient transport and photosynthesis, which are key for successful plant growth in vitro.
6) Table:
A 'table' in research presents data in a structured format, allowing for easy comparison and analysis. The tables in the study summarize the hormonal concentrations and corresponding callus induction percentages, contributing to the statistical representation of results, essential for validating the effectiveness of different treatment combinations.
7) Soil:
Soil is a natural resource composed of minerals, organic matter, air, and water that supports the growth of plants. Cowpea is known for its ability to grow in poor soil, making it an ideal crop for arid regions. Exploring soil conditions is vital for optimizing agricultural practices and enhancing yield.
8) Surface:
The term 'surface' commonly refers to the outermost layer of an object. In tissue culture, the surface of explants must be properly sterilized to prevent contamination before being placed in a growth medium. Contaminated surfaces can lead to failure in tissue culture experiments, making surface treatment critical.
9) Sharad (Sarad):
Sharad refers to one of the authors, whose study contributes valuable information on callus induction in cowpea using specific hormonal treatments. The research from Sharad Vats et al. assists in establishing a foundation for future studies focused on enhancing the nutritional and phytochemical composition of cowpea through tissue culture.
10) Family:
In biological classification, 'family' is a rank that groups together related plants or animals. Cowpea belongs to the Fabaceae family, which facilitates the understanding of its characteristics and relationships with other legumes. Knowledge of plant taxonomy is integral to breeding programs and conservation efforts.
11) Botany:
Botany is the scientific study of plants, including their physiology, structure, and genetics. The research conducted in the Department of Botany emphasizes the importance of understanding plant biology to improve crop species like cowpea, which are crucial for sustainable agriculture and nutritional security.
12) India:
India is a significant agricultural country where crops like cowpea are grown across diverse climatic zones. The research carried out in India highlights the country's role in enhancing agricultural practices and addressing nutritional needs, contributing to both local and global food security objectives.
13) Study (Studying):
A 'study' refers to a systematic investigation aimed at discovering or interpreting facts. In this context, the study on cowpea focuses on callus induction through tissue culture techniques. Research findings contribute to practical applications in agriculture, such as improving cultivation methods and enhancing crop yields.
14) Genu:
The term 'genus' refers to a taxonomic category ranking above species and below family. Cowpea is classified under the genus Vigna. Understanding the classification helps in studying evolutionary relationships among plants, aiding breeding and biotechnological innovations for crop improvement and sustainability.
15) Discussion:
The 'discussion' section of a research article analyzes and interprets the findings, placing them in the context of existing literature. This section provides insights into the implications of the study's results, potential applications in agriculture, and suggests areas for future research in plant regeneration and callus formation.
16) Seedling:
A 'seedling' is a young plant that develops from a seed. In the study, comparisons are made between callus derived from explants and seedling phytochemical content. Understanding seedling characteristics is crucial for crop management and understanding plant development processes, informing breeding and cultivation strategies.
17) Lakshmi (Laksmi):
Lakshmi refers to one of the authors of the study, contributing expertise to the research on cowpea tissue culture. The collaboration among researchers is important for interdisciplinary studies that enhance our understanding of plant biology, leading to improved agricultural practices and crop productivity.
18) Quality:
Quality in this context refers to the nutritional and overall value of the cowpea. The study emphasizes the importance of enhancing the quality of crops through tissue culture techniques, which can lead to improved growth rates, higher yields, and better nutritional profiles, addressing both consumer and agricultural needs.
19) Disease:
Disease in plants refers to any condition that impairs their normal functioning, often caused by pathogens. The study highlights that disease-free explants are essential for successful callus induction in tissue culture, as diseases can significantly hinder growth and affect the reliability of research findings.
20) Mineral:
Minerals are inorganic nutrients required by plants for various physiological functions, including growth and development. The research emphasizes the importance of including essential minerals in the growth medium during tissue culture, which influences callus induction and ultimately enhances the nutritional quality of the cowpea plant.
21) Science (Scientific):
Science is the systematic pursuit of knowledge through observation and experimentation. The study of cowpea tissue culture exemplifies applied science, bridging plant biology with agricultural practices. Scientific research plays a vital role in developing innovative solutions for improving crop traits and ensuring food security.
22) Animal:
In this context, 'animal' refers to livestock or animals that benefit from cowpea as fodder. Understanding the role of crops like cowpea in animal nutrition is important for sustainable agricultural practices and enhancing livestock productivity, thereby contributing to economic stability for farmers and communities.
23) Farmer:
A 'farmer' is an individual engaged in agriculture, cultivating crops for food production and economic sustainability. The study's focus on cowpea highlights its significance for farmers, especially in arid regions, as it serves as a vital source of income and nutrition, fostering sustainable farming practices.
24) Trader:
A 'trader' refers to an individual or entity involved in the buying and selling of agricultural products. The economic significance of cowpea for traders in the region emphasizes the crop's role in local and international markets, impacting food supply chains and economic development in agricultural communities.
25) Naga (Nag):
Naga refers to one of the researchers contributing to the study on cowpea tissue culture. Collaborative research efforts are crucial for generating comprehensive knowledge that can be applied to improve crop cultivation, enhance yield, and address agricultural challenges faced by farmers in various regions.
26) Rich (Rch):
In this context, 'rich' refers to the high nutritional value and flavour of cowpea, which provides essential vitamins and minerals beneficial for human and animal consumption. Emphasizing crops that are rich in nutrients is important for health and wellness, addressing food insecurity and malnutrition.
27) Pur:
The term 'poor' signifies regions with low nutrient availability, where crops like cowpea can thrive. Understanding how plants adapt to poor soil conditions is crucial for developing sustainable agriculture strategies and improving food security, particularly in arid and less-favoured areas of crop cultivation.
28) Dish (Dis):
'Dish' generally refers to a utensil for serving or cooking food. In the context of the study, the sterile Petri dish plays a critical role in plant tissue culture as it provides a controlled environment for callus induction, underscoring the importance of sterile techniques in scientific experiments.
Other Science Concepts:
Discover the significance of concepts within the article: ‘Callus induction from different explants in vigna unguculata l’. Further sources in the context of Science might help you critically compare this page with similair documents:
Nutrient medium, Tissue Culture, Photochemical analysis.
Concepts being referred in other categories, contexts and sources.