Phytochemical screening and analysis of Pergularia daemia leaves.

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Journal name: World Journal of Pharmaceutical Research
Original article title: Preliminary phytochemical screening and physicochemical analysis of leaves of pergularia daemia (forssk.) chiov.
The WJPR includes peer-reviewed publications such as scientific research papers, reports, review articles, company news, thesis reports and case studies in areas of Biology, Pharmaceutical industries and Chemical technology while incorporating ancient fields of knowledge such combining Ayurveda with scientific data.
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Author:

Sandhya Bhoyar and Sharad Biradar


World Journal of Pharmaceutical Research:

(An ISO 9001:2015 Certified International Journal)

Full text available for: Preliminary phytochemical screening and physicochemical analysis of leaves of pergularia daemia (forssk.) chiov.

Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research

Copyright (license): WJPR: All rights reserved


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Summary of article contents:

Introduction

The study focuses on Pergularia daemia (Forssk.) Chiov., a member of the Asclepiadaceae family, known for its traditional medicinal uses, including treatments for coughs and asthma. This research aimed to conduct a detailed examination of the phytochemical and physicochemical characteristics of P. daemia leaves, alongside their pharmacognostical attributes. The findings contribute to the understanding of this plant's medicinal potential and highlight the importance of herbal remedies, especially in developing countries, where many still rely on them as primary healthcare.

Phytochemical Screening

One of the primary objectives of the study was to perform phytochemical screenings on P. daemia leaves. The findings revealed a diverse array of phytochemicals, including alkaloids, flavonoids, tannins, terpenoids, saponins, carbohydrates, and amino acids. These compounds are significant as they possess various health benefits and can aid in the prevention and treatment of diseases. The research employed Soxhlet extraction with various solvents, such as petroleum ether, ethanol, methanol, and distilled water, which illustrated differences in the presence of these phytochemicals across extracts, particularly noting that alkaloids were prevalent in most but absent in the ethanol extract.

Microscopic and Morphological Analysis

The investigation included a thorough microscopic examination of the leaf structure, revealing distinct anatomical features crucial for identification and standardization of the herbal product. Key characteristics observed included the presence of a single-layered upper epidermis with various types of trichomes, a compact arrangement of palisade parenchyma cells, and defined vascular structures within the midrib. The stomatal morphology was also detailed, showing anisocytic types surrounded by three subsidiary cells. Such microscopic details aid in confirming the authenticity of the medicinal plant and understanding its physiological properties.

Physicochemical Properties

Physicochemical assessment comprised measuring parameters such as moisture content, total ash value, and extractive values to evaluate the quality and purity of the crude drug. The moisture content was noted to be 8.79%, with a total ash value of 24.1%. Furthermore, the alcohol and water-soluble extractive values were quantified at 23.2% and 35.2%, respectively. These measurements are essential for establishing standards that ensure the reliability and safety of herbal products, addressing potential issues of adulteration, and supporting their proper storage and use.

Conclusion

The comprehensive analysis of Pergularia daemia reveals vital pharmacognostical features that contribute to the plant's standardization and authenticity as a medicinal herb. The study underscores the significance of understanding the phytochemical composition, microscopic structure, and physicochemical properties to promote safe and effective use in traditional medicine. These findings serve as a foundation for further research and utilization of P. daemia in herbal pharmacotherapy, exemplifying the ongoing relevance of plant-based solutions in modern medicine.

FAQ section (important questions/answers):

What is the purpose of the study on Pergularia daemia leaves?

The study aims to screen various phytochemicals and analyze the physicochemical characteristics of Pergularia daemia leaves, known for their medicinal properties.

What are some traditional uses of Pergularia daemia?

Pergularia daemia is traditionally used to treat cough, asthma, rheumatic swelling, and various other ailments in the Ayurvedic system.

What kind of phytochemicals were found in the leaves?

The analysis revealed the presence of alkaloids, flavonoids, tannins, terpenoids, saponins, carbohydrates, and amino acids in the leaves of Pergularia daemia.

How was the physicochemical analysis of the leaves conducted?

The physicochemical analysis included measuring moisture content, total ash values, and the extractive values for both alcohol and water extracts.

What microscopic features were observed in Pergularia daemia leaves?

The microscopic analysis showed a single layered upper epidermis, palisade parenchyma, spongy mesophyll, and anisocytic stomata on both leaf surfaces.

Why is pharmacognostical study important for herbal drugs?

Pharmacognostical studies help in the identification, authentication, and detection of adulteration in crude drugs, ensuring their quality and safety.

Glossary definitions and references:

Scientific and Ayurvedic Glossary list for “Phytochemical screening and analysis of Pergularia daemia leaves.”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.

1) Drug:
The term 'drug' refers to any substance that, when introduced into the body, alters its physiological functions. In traditional medicine, herbal drugs derived from plants, such as Pergularia daemia, are often used for their therapeutic properties to treat ailments like cough and asthma, emphasizing the importance of drug development from natural sources.

2) Powder:
In the context of the research, 'powder' signifies the processed form of plant material, specifically the leaves of Pergularia daemia that have been dried and ground. The powdered form is essential for conducting various pharmacognostical and phytochemical analyses that evaluate the presence of active compounds and their effectiveness as therapeutic agents.

3) Phytochemical:
Phytochemicals are bioactive compounds derived from plants that possess various health benefits. In this study, multiple phytochemicals, including alkaloids, flavonoids, and saponins, were identified in the leaves of Pergularia daemia, indicating the plant's potential as a source for natural medicines. Understanding phytochemicals is crucial for developing herbal therapies.

4) Water:
Water plays a significant role in the extraction process in phytochemical studies. It is used as a solvent to extract soluble compounds from plant material. The water-soluble extractives of Pergularia daemia are quantified in the study, underscoring their importance in determining the quality and efficacy of herbal remedies.

5) Medicine:
Medicine encompasses a broad array of substances and practices used to prevent, diagnose, and treat illnesses. In this research, traditional herbal medicine is emphasized, showcasing the therapeutic applications of Pergularia daemia for conditions such as cough and asthma, alongside the importance of validating these effects through scientific study.

6) Table:
In scientific research, 'table' refers to a structured arrangement of data that facilitates analysis and comparison. The article presents various tables summarizing the findings, such as phytochemical screening results and physicochemical properties of Pergularia daemia, which are critical for assessing the quality and efficacy of herbal medicines.

7) Flavonoid:
Flavonoids are a class of phytochemicals known for their antioxidant properties and potential health benefits. The study identifies flavonoids as present in various extracts of Pergularia daemia, suggesting their role in the plant's medicinal effects. Their significance in combating diseases highlights the value of plant-derived compounds in treatment.

8) Study (Studying):
The term 'study' denotes systematic research aimed at exploring and understanding specific phenomena. In this context, the study investigates the pharmacognostical characteristics, physicochemical properties, and phytochemical composition of Pergularia daemia, contributing to the scientific validation and potential application of traditional herbal medicine in healthcare.

9) Disease:
Disease refers to a pathological condition that disrupts normal bodily functions. The study highlights the significance of herbal remedies like Pergularia daemia in treating diseases such as cough and asthma. Understanding the connection between traditional medicine and disease management underscores the relevance of ethnobotanical studies in modern healthcare.

10) Surface:
In botanical studies, 'surface' refers to the exterior aspects of plant tissues, such as leaves. The examination of surfaces includes analyzing trichomes and stomata distribution, which provide insights into the plant's physiology and its ability to interact with the environment, essential for understanding its medicinal properties.

11) Observation:
Observation in scientific research involves careful monitoring and recording of phenomena to gather data. The study emphasizes various observations during the microscopic analysis and phytochemical screening of Pergularia daemia, which are vital for validating findings and ensuring accurate characterization of the plant's therapeutic potential.

12) Sandhya (Shandhya, Samdhya):
Sandhya Bhoyar is a co-author of the study, contributing to the research on Pergularia daemia. As a researcher, her role includes conducting experiments, analyzing data, and compiling findings that advance the understanding of the plant's pharmacognostical and phytochemical properties, significant for herbal medicine development.

13) Botany:
Botany is the scientific study of plants, including their physiology, structure, and ecology. The research conducted by Sandhya Bhoyar and her team falls under the domain of botany as it investigates the properties of Pergularia daemia, emphasizing the importance of plant sciences in traditional and modern medicine.

14) India:
India is the geographical context for the research study, where Pergularia daemia is found growing naturally. The cultural and historical backdrop of herbal medicine in India adds depth to the research, highlighting the longstanding traditions in utilizing local flora for health and wellness, essential for understanding medical practices globally.

15) Post:
In the research context, 'post' could refer to the affiliation of the authors with academic institutions, thereby indicating their professional standing in the field of botany and pharmacognosy. This enhances the credibility and significance of the study as contributors to medicinal plant research.

16) Pharmacognostical:
Pharmacognostical relates to the study of medicinal drugs derived from natural sources. The research on Pergularia daemia involves pharmacognostical evaluation to assess its physical and chemical characteristics, which is crucial for standardization and ensuring the efficacy of herbal medicines used traditionally for various ailments.

17) Dysmenorrhea (Dysmenorrhoea):
Dysmenorrhea is a medical term for painful menstruation. The article mentions traditional uses of Pergularia daemia, implying that its consumption may alleviate symptoms associated with dysmenorrhea. The connection between herbal medicine and menstrual health highlights the therapeutic potential of plant-based treatments in women's health.

18) Maharashtra (Maharastra, Maha-rashtra):
Maharashtra is the state in India where the plant Pergularia daemia was collected for research. The regional context is important as it influences the availability and traditional uses of medicinal plants, underscoring the significance of local knowledge and biodiversity in the study of herbal remedies.

19) Discussion:
Discussion in research involves analyzing and interpreting data to draw conclusions. In this study, the discussion section likely covers the implications of the findings regarding the pharmacognostic and phytochemical properties of Pergularia daemia, providing insights into its potential medicinal applications based on the results obtained.

20) Developing:
Developing refers to the process of creating or enhancing something. In the context of this research, developing herbal medicines through the study of traditional plant use enriches the scientific foundation for future pharmaceutical applications. It reflects the ongoing efforts to bridge traditional knowledge and modern drug discovery.

21) Knowledge:
Knowledge encompasses the information and understanding derived from study and experience. The research aims to enrich the existing knowledge about Pergularia daemia, particularly its pharmacognostical and medicinal properties, thus contributing to the broader field of ethnopharmacology and the preservation of traditional medicine practices.

22) Observing:
Observing entails closely monitoring plant characteristics and behaviors to gather scientific data. The researchers in this study employ observing techniques during microscopic analysis and organoleptic evaluations to accurately document the features of Pergularia daemia. Such diligence is crucial for validating its medicinal relevance.

23) Swelling:
Swelling refers to the enlargement of body parts due to inflammation or injury. Traditional uses of Pergularia daemia include treatments for rheumatic swelling, showcasing the plant's relevance in managing inflammatory conditions. Understanding such therapeutic claims is essential for validating its application in integrative medicine.

24) Quality:
Quality refers to the standard or grade of a substance. In this study, the assessment of the quality of Pergularia daemia is emphasized through physicochemical analysis and extractive values. This evaluation is crucial for certifying the efficacy and safety of herbal formulations derived from the plant.

25) Killing (Killed):
Killing refers to the causing of death, especially in the study of diseases. While the text discusses herbal remedies combating harmful diseases, it emphasizes the importance of plants like Pergularia daemia in preventing the escalation of illnesses, supporting the broader narrative of traditional medicine's role in health care.

26) Sharad (Sarad):
Sharad Biradar is a co-author of the study, indicating collaboration in the research on Pergularia daemia. His contributions likely involve fieldwork, data analysis, and discussions relevant to the pharmacognostical and phytochemical assessment of the plant, showcasing teamwork in scientific inquiry.

27) Thomas:
Thomas refers to an author noted in the research, likely providing foundational knowledge or methodologies in pharmacognosy. The mention of his work underscores the collaborative nature of scientific research and builds upon existing literature for the evaluation of herbal medicines like Pergularia daemia.

28) Family:
Family refers to a taxonomic group in biology. In this context, it pertains to the family Asclepidaceae, to which Pergularia daemia belongs. The classification is vital for understanding the ecological and medicinal traits shared by species within the same family, shaping the study's focus on traditional uses.

29) Purity:
Purity is a critical aspect of pharmacognostical studies, denoting the absence of adulterants and contextual factors that could compromise the quality of herbal drugs. Ensuring the purity of Pergularia daemia enhances its medicinal efficacy and safety, which is essential for both traditional and modern health practices.

30) Kumar:
Kumar refers to an author referenced in the research, suggesting his contributions to the study of pharmacognosy. His prior works inform the methodologies applied in this study, reinforcing the collaborative nature of research and the accumulation of knowledge in the field of herbal medicine.

31) Ulcer:
Ulcer refers to a sore that develops on the skin or mucous membranes as a result of inflammation. The study hints at the traditional use of Pergularia daemia for gastric ulcers, highlighting the potential for herbal remedies in treating gastrointestinal ailments, thereby linking traditional practices with modern health concerns.

32) Fever:
Fever is a medical condition characterized by an elevated body temperature, often indicating infection. The article notes the traditional uses of Pergularia daemia in treating fever, reflecting the reliance on herbal medicine in symptom management and emphasizing the need for scientific validation of these traditional claims.

33) Pune:
Pune is a city in India where the Botanical Survey of India, which authenticated the plant used in the study, is located. This urban setting is significant as it facilitates research on local flora, contributing to the understanding of traditional medicinal practices and biodiversity in the region.

34) Tree:
Tree refers to a perennial plant with an elongated stem or trunk. In the case of Pergularia daemia, the plant may often grow on trees, highlighting its ecological interaction and dependence within its habitat. Such information is relevant for understanding its growth patterns and potential medicinal applications.

35) Hair:
Hair could refer to the trichomes found on plant surfaces, which are microscopic structures contributing to a plant's physical characteristics. In the context of the study, understanding the presence and role of trichomes can provide insights into the overall health and medicinal qualities of Pergularia daemia.

36) Hand:
Hand may refer to the involvement of researchers in the detailed analysis and hands-on practices during the study. It emphasizes the practical aspects of botanical research and the importance of careful handling and preparation of plant materials for achieving reliable and valid results.

37) Milk:
Milk is mentioned alongside the discussion of herbal remedies, as it is commonly used as a medium to prepare herbal infusions or decoctions. The link between milk and herbal medicine reflects traditional practices, suggesting that certain phytochemicals in plants like Pergularia daemia may be better absorbed or utilized when combined with other substances.

Other Science Concepts:

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Discover the significance of concepts within the article: ‘Phytochemical screening and analysis of Pergularia daemia leaves.’. Further sources in the context of Science might help you critically compare this page with similair documents:

Phytochemical, Traditional medicine, Herbal medicine, Preliminary phytochemical screening, Phytochemical screening, Pharmacognostic study, Physicochemical analysis, Microscopical Examination, Organoleptic character, Microscopic studies, Extractive value, Pharmacognostical characteristics, Phytochemical component.

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