Antioxidant, anti-inflammatory, and antimicrobial properties of Algerian lectins.
morus nigra, ruta graveolens, cyperus rotundus and pistacia lentiscus.
Journal name: World Journal of Pharmaceutical Research
Original article title: Antioxidant, anti-inflammatory and antimicrobial properties of new lectins purified from roots of algerian plants
The WJPR includes peer-reviewed publications such as scientific research papers, reports, review articles, company news, thesis reports and case studies in areas of Biology, Pharmaceutical industries and Chemical technology while incorporating ancient fields of knowledge such combining Ayurveda with scientific data.
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Subtitle: morus nigra, ruta graveolens, cyperus rotundus and pistacia lentiscus.
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Ahlem Bahi, Youcef Necib, Fateh Merouane, Hala Bouadi and Khaled Boulahrouf
World Journal of Pharmaceutical Research:
(An ISO 9001:2015 Certified International Journal)
Full text available for: Antioxidant, anti-inflammatory and antimicrobial properties of new lectins purified from roots of algerian plants
Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research
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Summary of article contents:
Introduction
The research investigates the antioxidant, anti-inflammatory, and antimicrobial properties of lectins purified from the roots of Algerian plants: Morus nigra, Ruta graveolens, Cyperus rotundus, and Pistacia lentiscus. These lectins were purified using desialylated erythrocyte stroma as a cost-effective method. The study aims to evaluate the antioxidant potential through various in vitro assays, examine the lectins' effect on inflammation in vivo, and assess their antimicrobial capabilities against various pathogens.
Antioxidant Properties of Purified Lectins
The antioxidant activities of the purified lectins were extensively tested using several assays, including DPPH radical scavenging, superoxide dismutase (SOD), ferric reducing power, hydrogen peroxide scavenging, and inhibition of lipid peroxidation. Among the lectins tested, those from Morus nigra and Ruta graveolens demonstrated significant antioxidant activities, exhibiting the lowest IC50 values compared to other lectins and ascorbic acid, a known standard antioxidant. Specifically, Morus nigra showed the strongest DPPH radical scavenging ability with an IC50 of 115.34 µg/ml, while Ruta graveolens followed closely with an IC50 of 208.95 µg/ml, indicating their potential for preventing oxidative stress-related cellular damage.
Anti-Inflammatory Effects
In addition to their antioxidant properties, the lectins displayed notable anti-inflammatory effects in an LPS-induced edema model in rats. The administration of the purified lectins (1.5 mg/kg) significantly reduced paw swelling compared to the control group, indicating their capacity to inhibit inflammatory responses. The lectins from Morus nigra and Ruta graveolens were particularly effective, showing a profound reduction in edema levels, suggesting their potential therapeutic benefits in treating inflammation-related disorders.
Antimicrobial Activity
The antimicrobial efficacy of the purified lectins was tested against various bacterial and fungal strains, including Escherichia coli, Staphylococcus aureus, and Candida albicans. The results indicated that the lectins from Morus nigra, Ruta graveolens, and Cyperus rotundus had varying degrees of antimicrobial activity, particularly against Bacillus cereus and certain fungi like Aspergillus flavus. However, they exhibited no activity against other tested pathogens, emphasizing the need for further research to understand the specific mechanisms and broader applicability of these lectins as antimicrobial agents.
Conclusion
The study highlights the promising antioxidant, anti-inflammatory, and antimicrobial properties of lectins purified from Algerian plant roots. The significant antioxidant activity observed, particularly in Morus nigra and Ruta graveolens, alongside their notable anti-inflammatory effects, positions these plant-derived lectins as potential candidates for therapeutic applications. Although their antimicrobial activity was limited to certain strains, continuing research may uncover new avenues for their use in medicine, particularly in developing natural alternatives to synthetic agents for combating oxidative stress and inflammation-related diseases.
FAQ section (important questions/answers):
What are the primary properties of the lectins studied?
The lectins purified from roots of Morus nigra, Ruta graveolens, Cyperus rotundus, and Pistacia lentiscus exhibit significant antioxidant, antimicrobial, and anti-inflammatory properties.
How were the lectins isolated from the plants?
The lectins were isolated using a method involving desialylated stroma from erythrocytes, followed by chromatography with Sephadex G-25 to purify the lectins from the plant roots.
What methods were used to evaluate antioxidant activities?
Antioxidant activities were evaluated using various assays including DPPH free radical scavenging, superoxide anion scavenging, ferric reducing power, and H2O2 scavenging, alongside lipid peroxidation inhibition tests.
Did the lectins show any anti-inflammatory properties?
Yes, the lectins demonstrated anti-inflammatory effects in LPS-induced edema in rats, significantly reducing paw swelling compared to control groups.
What was observed regarding antimicrobial activity?
The lectins showed limited antimicrobial activity, inhibiting only certain strains like Bacillus cereus, while showing no activity against others like Escherichia coli and Staphylococcus aureus.
What is the significance of the IC50 values reported?
IC50 values indicate the concentration required for 50% inhibition of free radicals, with lower values showing greater antioxidant potency; values varied across the lectins studied.
Glossary definitions and references:
Scientific and Ayurvedic Glossary list for “Antioxidant, anti-inflammatory, and antimicrobial properties of Algerian lectins.”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.
1) Activity:
Activity refers to the functional capacity or biological effects demonstrated by substances, such as the antioxidant, anti-inflammatory, and antimicrobial properties exhibited by lectins. In pharmacology and biochemistry, assessing activity is crucial for determining potential therapeutic applications and understanding how compounds interact with biological systems.
2) Cyperus rotundus:
Cyperus rotundus, known as purple nutsedge, is a plant with significant medicinal properties. It has been studied for its antioxidant, anti-inflammatory, and antimicrobial activities. Extracts from this plant have shown potential benefits in traditional medicine, making it relevant in research for developing natural therapeutics.
3) Table:
In scientific literature, a table presents data in an organized format, facilitating easy comparison and analysis. Tables summarize experimental results, such as protein concentrations, IC50 values, and other measured parameters, providing a clear visual representation of findings that aid in understanding the results of research studies.
4) Edema (Oedema):
Edema refers to the swelling caused by excess fluid accumulation in tissues, often resulting from inflammation or injury. In pharmacological studies, controlling edema is critical for evaluating the effectiveness of anti-inflammatory drugs and natural products, as well as understanding the mechanisms behind inflammatory responses in experimental models.
5) Drug:
A drug is a substance used for diagnosis, treatment, or prevention of diseases. It can be synthetic or derived from natural sources, such as plants. In the context of the study, drugs are evaluated based on their therapeutic effects, such as reducing inflammation or microbial growth, highlighting their importance in medicine.
6) Antibiotic (Antibacterial):
Antibacterial agents are substances that inhibit the growth of bacteria or kill bacteria. They are vital in preventing and treating bacterial infections. Research on antibacterial properties of natural compounds, like lectins from plants, helps in discovering alternative therapies in the face of increasing antibiotic resistance.
7) Inflammation:
Inflammation is the body's immune response to harmful stimuli, such as pathogens, damaged cells, or irritants. While a necessary protective mechanism, excessive inflammation can lead to chronic diseases. Understanding anti-inflammatory properties of compounds can lead to better treatments for inflammatory conditions, which is a focus in pharmacological research.
8) Food:
Food is essential for life, providing the necessary nutrients and energy for growth, maintenance, and overall health. In the context of antioxidants and natural compounds, food sources with high antioxidant properties can contribute to health benefits, lowering the risk of diseases, and enhancing well-being.
9) Purification:
Purification involves the isolation of specific substances to eliminate impurities. In laboratory contexts, it is crucial for obtaining pure compounds for research. For example, lectin purification from plant roots enables the study of specific biological activities without interference from other components, aiding in understanding their properties.
10) Water:
Water is a fundamental solvent and medium in biological processes, crucial for life. It participates in biochemical reactions, maintains cell structure, and regulates body temperature. In research, the role of water as a solvent is paramount, influencing extraction processes and the solubility of bioactive compounds.
11) Life:
Life encompasses the characteristic processes and phenomena that distinguish living organisms from inorganic matter. In science, studying life involves exploring various biological functions, such as growth, reproduction, and adaptation. Investigating compounds that influence life processes, like antioxidants, is essential for improving health and longevity.
12) Science (Scientific):
Science is the systematic study of the structure and behavior of the physical and natural world through observation and experimentation. It comprises various disciplines, including biology, chemistry, and medicine. Scientific research aims to discover new knowledge and improve understanding of health-related issues, including disease treatment.
13) Animal:
Animals are multicellular organisms that play vital roles in ecosystems. They are used in scientific research to model human diseases and test treatments. Studies on animal models help evaluate the efficacy of drugs, including anti-inflammatory and antimicrobial properties, and aid in understanding biological processes relevant to humans.
14) Toxicity:
Toxicity refers to the degree to which a substance can harm organisms. Understanding toxicity is crucial in evaluating the safety of drugs and natural compounds. Research aims to identify safe therapeutic doses for human use, minimizing adverse effects while maximizing therapeutic benefits in treating diseases.
15) Disease:
Disease is a disordered or abnormal condition of a biological system, often resulting in specific signs and symptoms. Disease research focuses on understanding etiologies, mechanisms, and treatments, enabling development of effective therapies that enhance quality of life and target preventive measures to improve overall health outcomes.
16) Aureus:
Aureus, particularly Staphylococcus aureus, is a common bacterium that can cause various infections. Understanding its pathogenicity and resistance mechanisms is crucial in developing effective antibacterial agents. Research focuses on natural and synthetic compounds' effectiveness against S. aureus to combat antibiotic resistance and improve treatment options.
17) Study (Studying):
A study refers to systematic investigation of a subject to establish facts or principles. In scientific research, studies are designed to test hypotheses and gather data about specific phenomena. Findings from studies contribute to advancing knowledge in disciplines such as pharmacology and medicine, impacting health strategies.
18) Human body:
The human body is a complex organism composed of various systems that work together to maintain life. Understanding its biological processes is vital for developing medical treatments. Research into herbs and compounds affecting human physiology can lead to new therapies for improving health and preventing diseases.
19) Surrounding:
Surrounding refers to the environment around an organism. It encompasses various external factors, including physical conditions, toxins, and interactions with other organisms. In biomedical research, understanding environmental effects on biological systems is crucial for comprehending health outcomes and the efficacy of treatments.
20) Measurement:
Measurement is the process of obtaining the magnitude, quantity, or dimension of a property. In scientific studies, accurate measurement is essential for assessing effects, such as antioxidant activity or protein concentration. It ensures data validity and reliability, contributing to the overall quality of research findings.
21) Discussion:
Discussion is a critical component of scientific papers where researchers interpret findings, compare results with previous studies, and suggest implications. It provides a platform for analyzing data within the broader context of the field, highlighting significance and encouraging further research based on observed outcomes.
22) Medicine:
Medicine is the science and practice of diagnosing, treating, and preventing illness. With advances in research, medicine incorporates natural compounds and traditional remedies alongside modern techniques. Understanding the properties of naturally derived substances can enhance therapeutic options and contribute to holistic healthcare approaches.
23) Swelling:
Swelling is the enlargement of body parts due to fluid accumulation or inflammation. It is a common symptom in various conditions and is often indicative of underlying issues. Understanding the mechanisms of swelling, such as those induced by inflammation, helps develop effective treatment strategies.
24) Relative:
Relative refers to a comparative relationship between two entities. In scientific research, relative measurements help understand the effects of tested compounds against controls or standards. They provide context for evaluating efficacy, safety, and therapeutic potential in developed treatments or interventions.
25) Quality:
Quality refers to the inherent characteristics of a product that determine its suitability for use. In medicine and food science, quality assessments ensure that compounds meet safety and efficacy standards. High-quality substances enhance therapeutic effects while minimizing risks to health, emphasizing the importance of rigorous testing.
26) Species:
Species is a fundamental unit in biological classification, representing a group of organisms capable of interbreeding. Understanding different species is crucial in pharmacological research, as various plants or microorganisms may offer distinct therapeutic properties. Species diversity contributes to the discovery of novel compounds for medical applications.
27) Surface:
Surface refers to the outermost layer of an object or organism, playing an essential role in interactions with the environment. In biomedicine, surface characteristics of cells and tissues affect drug delivery and efficacy. Understanding these interactions can inform the development of better therapeutic strategies.
28) Cancer:
Cancer is a group of diseases characterized by uncontrolled cell growth and division. Research aims to understand its mechanisms, identify potential treatments, and improve patient outcomes. Investigating natural compounds with anticancer properties provides alternative therapeutic approaches that may work synergistically with conventional treatments.
29) Medium:
Medium refers to a substance or environment in which biological reactions or processes occur. It influences the behavior and interactions of cells and compounds. In scientific contexts, selecting the appropriate medium for experiments is essential for achieving accurate and reproducible results, particularly in microbiological studies.
30) Sugar:
Sugar, a simple carbohydrate, is a crucial energy source for living organisms. It is involved in various biochemical processes, including metabolism and energy production. In the context of lectin research, sugar interactions with proteins can influence biological activities, highlighting the importance of understanding glycoprotein properties.
31) Blood:
Blood is the fluid that circulates in the body, transporting nutrients, gases, and waste products. It plays a critical role in maintaining homeostasis and immune response. In research, studying blood interactions with therapeutic agents helps evaluate the efficacy and safety of pharmacological treatments.
32) Hala:
Hala is a name referencing one of the authors in the study. Authors contribute significantly to the research, providing insight, expertise, and collaborative effort in exploring the properties of lectins in plants. Their collaboration enhances the credibility and scope of the study's findings.
33) Hind:
Hind refers to the rear part of an organism, particularly concerning limbs. In an anatomical or physiological research context, examining hind limb responses in animal studies can provide insights into the effects of treatments, such as changes in swelling or inflammation following drug administration.
34) Mast:
Mast typically refers to mast cells, which are specialized immune cells involved in allergic and inflammatory responses. In the context of inflammation research, understanding mast cell function helps decipher immune mechanisms and identifying potential therapeutic targets for managing allergic reactions and chronic inflammation.
35) Lamp:
A lamp refers to a source of light. In laboratory experiments, lamps provide necessary illumination for observing reactions and processes or stimulating cells. Proper lighting conditions are essential for accurate readings and successful execution of experiments that may involve light-sensitive substances.
36) Hand:
Hand refers to the human limb, often involved in manual tasks. In research, examining the effects of treatments on hand function or swelling can reveal therapeutic impacts on inflammatory or degenerative conditions, emphasizing the importance of evaluating physical capabilities in clinical studies.
37) Dish (Dis):
Dish refers to a shallow, flat container used in laboratories for culturing cells or microorganisms. It is crucial for conducting microbiological assays and tests. Using sterile dishes ensures accurate assessments of antimicrobial or antifungal activities, promoting valid scientific findings in laboratory research.
Other Science Concepts:
Discover the significance of concepts within the article: ‘Antioxidant, anti-inflammatory, and antimicrobial properties of Algerian lectins.’. Further sources in the context of Science might help you critically compare this page with similair documents:
Cyperus rotundus, Pistacia lentiscus, Ruta graveolens, Anti-inflammatory activity, Oxidative stress, In vitro, Zone of inhibition, DPPH assay, Antioxidant potential, Ascorbic acid, Scavenging activity, Microbial strains, H2O2 scavenging, Bacillus cereus, Natural antioxidant, C. albicans, SOD assay, Lipid peroxidation inhibition, Hydrogen donors, Electron donors.