Evaluation of anti-tumor agents in Annona muricata leaves.
Journal name: World Journal of Pharmaceutical Research
Original article title: Evaluation of the anti-tumour agents in annona muricata (linn.) leaves using column chromatography and gas chromatography - mass spectrometry
The WJPR includes peer-reviewed publications such as scientific research papers, reports, review articles, company news, thesis reports and case studies in areas of Biology, Pharmaceutical industries and Chemical technology while incorporating ancient fields of knowledge such combining Ayurveda with scientific data.
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Cynthia Ekwy Ogukwe, Chris O. Akalezi, Brendan Okechukwu Ezugwu and Henry Ebuka Ojukwu
World Journal of Pharmaceutical Research:
(An ISO 9001:2015 Certified International Journal)
Full text available for: Evaluation of the anti-tumour agents in annona muricata (linn.) leaves using column chromatography and gas chromatography - mass spectrometry
Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research
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Summary of article contents:
Introduction
Annona muricata, known as soursop, is a tropical fruit belonging to the Annonacae family, popular for its therapeutic properties. The fruit is traditionally used in various regions, including tropical America, Australia, Africa, and Malaysia for its numerous health benefits, attributed to its rich nutrient profile and bioactive compounds. This study aims to evaluate the anti-tumor properties of ethanol extracts from the leaves of Annona muricata through column chromatography and Gas Chromatography-Mass Spectrometry (GC-MS) analysis, aiming to understand its potential medicinal applications, particularly in the treatment of tumors and related ailments.
Phytocomponents Identified in Ethanol Extract
The GC-MS analysis of the ethanol extract of Annona muricata leaves revealed the presence of 45 distinct phytocomponents. Notable compounds identified include 1-Pentadecene, 1-Nonadecene, and hexadecenoic acid methyl ester, along with several long-chain fatty acids and alcohols. These compounds were found in varying concentrations, with some, like Bis(2-ethylhexyl) phthalate showing a high percentage of 7.63%, indicating its potential for biological activities. The chemical presence in the extract suggests significant environmental and medicinal applications, particularly in combating various diseases due to their bioactive nature.
Antitumor and Pharmacological Properties
The study highlights the probable use of the identified phytocomponents in ethnomedicine for treating tumors. Key compounds such as n-Tetracosanol-1 and n-Nonadecanol-1 demonstrated anti-cancer properties, while others exhibited antimicrobial, antioxidant, and anti-inflammatory activities. The presence of these bioactive compounds supports the traditional claims regarding the therapeutic effectiveness of Annona muricata leaves, providing a basis for further research into their specific mechanisms and potential as drug candidates.
Traditional Uses and Nutritional Benefits
Historically, various parts of Annona muricata, including its leaves, fruit, and seeds, have been used in traditional medicine for ailments ranging from headaches and insomnia to diabetes and rheumatism. The fruit is not only a source of carbohydrates but is also rich in vitamins and minerals, adding to its nutritional appeal. This study supports the relevance of soursop within dietary practices and highlights its essential role in community health, emphasizing the need for more rigorous scientific studies to validate traditional remedies.
Conclusion
This research indicates that the ethanol extract of Annona muricata leaves contains a wealth of bioactive compounds, suggesting promising pharmacological activities that bolster its traditional medical use, particularly in treating tumors. The findings provide a scientific basis for further exploration and validation of the plant’s medicinal properties. Encouraging further studies on individual phytochemical constituents may pave the way for discovering new drugs and enhancing understanding of Annona muricata’s role in contemporary medicine. Continued investigation into the efficacy and safety of these compounds is essential to potentially harness their full therapeutic potential.
FAQ section (important questions/answers):
What is the purpose of the study on Annona muricata leaves?
The study aimed to evaluate the anti-tumour properties of ethanol extract from Annona muricata leaves using column chromatography and gas chromatography-mass spectrometry (GC-MS) analysis.
What methods were used to analyze the Annona muricata leaves?
The researchers applied column chromatography for separation and purification, followed by gas chromatography-mass spectrometry (GC-MS) to identify and analyze the phyto-compounds present in the ethanol extract of the leaves.
What types of compounds were found in the extract?
The GC-MS analysis revealed 45 phytocomponents, including long-chain fatty acids, alcohols, and esters, with notable compounds being 1-Pentadecene, n-Tetracosanol-1, and Bis(2-ethylhexyl) phthalate, among others.
How were the plant materials prepared for extraction?
Fresh Annona muricata leaves were dried, pulverized, and then treated with absolute ethanol for 48 hours before filtration. The filtrate was concentrated to dryness for further purification.
What medicinal uses of Annona muricata are mentioned in the study?
The leaves are reported to have various medicinal uses, including treating headaches, insomnia, hypertension, and tumors, highlighting their potential ethnomedicinal applications.
What further research is suggested based on the study findings?
The study suggests further research on isolating individual phytochemical constituents and conducting biological activity and toxicity tests to develop new drug formulations derived from Annona muricata.
Glossary definitions and references:
Scientific and Ayurvedic Glossary list for “Evaluation of anti-tumor agents in Annona muricata leaves.”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.
1) Activity:
Activity refers to the specific biological effects exhibited by certain compounds found in plants, such as those extracted from Annona muricata. In this context, it highlights how phytochemicals contribute to medicinal effects, such as anti-tumoral, antimicrobial, and antioxidant properties, which are essential for evaluating therapeutic potential.
2) Disease:
Disease indicates any abnormal condition affecting the body, which the phytocompounds in Annona muricata may address. Various diseases, including cancer, diabetes, and infections, are mentioned, and the study aims to substantiate the plant's traditional medicinal uses in treating these ailments, emphasizing the importance of medicinal plants in healthcare.
3) Table:
In the research, tables are used to systematically summarize data like phytocompound identification, retention time, and percentage composition from the GC-MS analysis. They provide a clear visual representation of findings, allowing for easy comparison and reference, which increases the study's comprehensibility and aids further discussions.
4) Antibiotic (Antibacterial):
Antibiotic denotes substances that kill or inhibit the growth of bacteria. The study of Annona muricata investigates its phytochemicals that may exert antibiotic effects, supporting the relevance of this plant in addressing bacterial infections and emphasizing the importance of natural antibiotic sources in treatment.
5) Family:
Family refers to a taxonomic category in biological classification. In this context, Annona muricata belongs to the Annonaceae family. Understanding the family helps identify shared characteristics and properties of plants, which can lead to exploring their collective medicinal benefits and potential pharmacological applications.
6) Study (Studying):
Study signifies the research conducted to evaluate the anti-tumour properties of Annona muricata leaves. It reflects the structured investigation into the plant's potential therapeutic effects, utilizing methods such as GC-MS. The study contributes scientific insights into traditional medicine practices and supports the need for further research.
7) Pharmacological:
Pharmacological pertains to the study of drugs and their effects on the body. In this research, it relates to the evaluation of compounds' therapeutic effects. Understanding the pharmacological properties of phytochemicals from plants like Annona muricata is essential for developing new medications and exploring their possible health benefits.
8) Medicine:
Medicine encompasses the science and practice of diagnosing, treating, and preventing disease. The interactions between phytochemicals present in Annona muricata and various health conditions highlight the plant’s potential role in modern and traditional medicine, contributing to the search for new treatments and healthcare solutions.
9) Species:
Species refers to a specific group of organisms capable of interbreeding. In this research, Annona muricata is highlighted as a notable species with potential health benefits. An understanding of the species contributes to conservation efforts and the exploration of biodiversity's role in traditional medicine systems.
10) Worm:
Worm refers to parasitic organisms that can infect humans and animals. This study mentions traditional uses of Annona muricata against worms, particularly for its potential anthelmintic properties. Researching this aspect can lead to effective treatments for parasitic infections, enhancing public health.
11) Phytochemical:
Phytochemical refers to the bioactive compounds derived from plants. In the context of the study, the identification of phytochemicals presents insights into their potential health benefits, illustrating how these natural compounds can be harnessed for medicinal uses and further developed into therapeutic agents.
12) Fragrance:
Fragrance refers to a sweet or pleasant smell often associated with flowers and plants. In the context of Annona muricata, fragrances can indicate certain phytochemicals used in perfumes or as natural scent contributions. Exploring fragrance components also reveals potential commercial and therapeutic applications.
13) Pesticide:
Pesticide relates to substances utilized to repel or kill pests, including insects and weeds. The study's reference to pesticides highlights the broader application of compounds isolated from plants like Annona muricata, illustrating their potential in agriculture for natural pest control, reducing reliance on synthetic chemicals.
14) Cancer:
Cancer refers to a disease characterized by uncontrolled cell growth. The research emphasizes the anti-tumour properties of Annona muricata, focusing on how its phytochemicals may provide therapeutic options for cancer treatment, aligning with the necessity of finding effective and less toxic cancer therapies.
15) Water:
Water is essential for various biological processes and is a critical solvent in the extraction and preparation of plant materials. In this study, water is implied in methods of preparing extracts and may also relate to the biological systems where medicinal plants exhibit their effects.
16) Genu:
Genu is likely a typographic error and may refer to 'genus.' In botanical classification, genus encompasses groups of species that are closely related. Understanding the genus of Annona muricata aids in recognizing its relatives and their shared properties, thus enriching research into plant-based therapies.
17) Drug:
Drug signifies a substance that can alter physiological processes in body organisms. The study aims to assess the potential transformative properties of phytochemicals from Annona muricata as therapeutic agents, thus contributing to drug development and enhancing healthcare possibilities through natural product research.
18) Human life:
Human life encapsulates the biological and social aspects of human existence. The study's exploration of Annona muricata aims to enhance health outcomes, reflecting on the importance of using natural resources to improve the quality of human life by discovering potential treatments for diseases.
19) Purification:
Purification involves the separation and isolation of specific compounds from mixtures. In this research, the process of column chromatography was used to purify the extract to identify bioactive compounds effectively. Purification is vital for understanding the specific actions of isolated phytochemicals and their potential medical applications.
20) Discussion:
Discussion refers to the examination and interpretation of findings within the study. It provides an essential context for understanding the implications of results, contributing to scientific discourse, and guiding future research directions centered on the pharmacological properties of Annona muricata.
21) Developing:
Developing implies the process of creating or improving. In the context of this research, it relates to the ongoing efforts to investigate the potential applications of phytochemicals from Annona muricata for therapeutic uses, aiming to innovate and discover new treatments for various health conditions.
22) Science (Scientific):
Scientific refers to the systematic study of the natural world through observation and experimentation. This research employs scientific methods to analyze the phytochemical properties of Annona muricata, providing evidence-based insights that contribute to the credibility and validation of traditional uses in medicine.
23) Flavonoid:
Flavonoid represents a class of phytochemicals known for their antioxidant and anti-inflammatory properties. While not explicitly mentioned as a key component of Annona muricata, understanding flavonoids is crucial for evaluating the holistic benefits of the plant and its potential roles in disease prevention and health enhancement.
24) Toxicity:
Toxicity refers to the degree to which a substance can harm organisms. Studies on phytochemicals must include toxicity assessments to ensure that any therapeutic agents derived from Annona muricata are safe for use. Evaluating toxicity is essential for drug development and ensuring patient safety.
25) Training:
Training indicates the education and skill development necessary for researchers to utilize analytical equipment and methodologies. The acknowledgment of training facilities in this study highlights the importance of technical skills in conducting research effectively and producing reliable scientific data during the study.
26) Relative:
Relative refers to a comparative measure or relationship between entities. In the context of pharmacological studies, understanding the relative abundance of various compounds can provide insight into the biological significance of phytochemicals from Annona muricata, indicating their potential efficacy in therapeutic applications.
27) Surface:
Surface can refer to the outermost layer of a substance where interactions take place. In chemical and biological contexts, surface properties of materials, such as plant cell walls, can influence extraction methods, compound activity, and effectiveness in interactions with biological systems, relevant for analyzing phytochemicals.
28) Aureus:
Aureus commonly refers to Staphylococcus aureus, a type of bacteria known for causing infections. The presence of antibacterial activity against Staphylococcus aureus in the study illustrates the significance of Annona muricata as a potential natural treatment for combating bacterial infections affecting humans.
29) Bitter:
Bitter describes a taste characteristic often found in certain plant parts, which can indicate the presence of specific compounds. The bitter rind of Annona muricata is mentioned, highlighting how perception of taste can be linked to bioactivity and the varied applications of plant-derived substances in medicine and food.
30) Nature:
Nature denotes the physical world and organisms, including flora and fauna, which contribute to biodiversity. In the context of this research, understanding nature emphasizes the importance of exploring natural products, such as Annona muricata, which hold significant potential for developing new pharmacological therapies.
31) Powder:
Powder refers to a finely ground substance, often prepared from plant material for extraction purposes. The study mentions the pulverization of Annona muricata leaves, which is crucial in the extraction methods to increase the surface area and improve the yield of bioactive compounds for analysis.
32) Yunnan:
Yunnan is a province in China known for its diverse flora, including Annona muricata. The reference to Yunnan illustrates the geographic significance of harvesting plants with medicinal properties and encourages exploration of local plants in traditional medicine for broader applications in health and nutrition.
33) Candy:
Candy typically refers to sweet confections made from sugar or syrup often associated with fruit flavors. The mention of candy in relation to Annona muricata suggests the fruit's culinary uses, promoting the integration of functional foods into diets and demonstrating the versatility of medicinal plants.
34) Glass:
Glass refers to a material often used to make laboratory equipment, such as vials for collecting and analyzing samples. In this research, glass vials were used to store fractions from column chromatography, highlighting the importance of appropriate materials in analytical chemistry for preserving sample integrity.
35) Cina:
China is a country regarded for its rich biodiversity and traditional medicine practices. The reference to China in relation to Annona muricata points to the importance of cultural and geographic contexts in studying herbal remedies, fostering global collaboration in the exploration of medicinal plants.
36) Food:
Food denotes the substances consumed to provide nutritional support for the body. The context in this research emphasizes the dual role of Annona muricata as nutritional sustenance and a source of bioactive compounds, highlighting the significance of food plants in promoting health and well-being.
37) Amde (Ande):
Ande is potentially a typographical or contextual error and may refer to the Andean region's relation to the research on Annona muricata. Acknowledging geographical regions can enhance the understanding of plant distribution, cultural usages, and the integration of traditional knowledge into modern scientific research.
38) Tree:
Tree refers to a perennial plant with an elongated stem or trunk. Annona muricata is described as a tree, emphasizing the ecological and practical roles of such species in providing food, medicinal resources, and habitats while reflecting the importance of plant biodiversity in human well-being.
39) Rich (Rch):
Rich denotes an abundance or strong presence of particular qualities or resources. In this research, the term highlights the high concentration of bioactive phytochemicals in Annona muricata leaves, thus indicating the potential for health benefits and the importance of exploring such natural resources for therapeutic purposes.
40) Life:
Life encompasses the characteristics differentiating living organisms, highlighting the interconnectedness of ecosystems. The discussion on Annona muricata emphasizes the role of plants in sustaining human life through nutrition and medicine, showcasing the significance of respecting and safeguarding biodiversity and ecological balance for future generations.
Other Science Concepts:
Discover the significance of concepts within the article: ‘Evaluation of anti-tumor agents in Annona muricata leaves.’. Further sources in the context of Science might help you critically compare this page with similair documents:
Traditional medicine, Medicinal use, Anti-inflammatory, Antimicrobial activity, Ethno-medicine, Ethanol extract, Column chromatography, Nutraceutical, Biological activities, Gas Chromatography Mass Spectrometry, Biomedical application, Fractional analysis, Phytochemical constituent.