In silico characterization of QTLs for GPC and micronutrient content.

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Journal name: World Journal of Pharmaceutical Research
Original article title: In silico structural and functional characterization of qtls controlling gpc and micronutrient content and identification of candidate gene based qtls specific markers
The WJPR includes peer-reviewed publications such as scientific research papers, reports, review articles, company news, thesis reports and case studies in areas of Biology, Pharmaceutical industries and Chemical technology while incorporating ancient fields of knowledge such combining Ayurveda with scientific data.
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Author:

R. K. Zote, G. Chandel, R. N. Dhawale, S. B. Verulkar and A. S. Kotasthane


World Journal of Pharmaceutical Research:

(An ISO 9001:2015 Certified International Journal)

Full text available for: In silico structural and functional characterization of qtls controlling gpc and micronutrient content and identification of candidate gene based qtls specific markers

Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research

Copyright (license): WJPR: All rights reserved


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Summary of article contents:

1) Introduction

Rice is a crucial staple food for over half of the world's population, particularly in developing countries where micronutrient deficiencies are prevalent. The study focuses on the complex polygenic traits associated with iron (Fe) and zinc (Zn) content in rice grains, which are essential for addressing malnutrition. The authors aimed to identify quantitative trait loci (QTLs) and develop molecular markers that can aid in biofortification efforts to enhance the micronutrient content of rice through marker-assisted breeding techniques.

2) Identification of QTLs and Molecular Markers

The research involved conducting co-segregation analysis with a mapping population derived from the cross between the rice cultivars Swarna and Moroberekan. The study identified five known QTLs (qFE-1, qFE-9, qZN-5, qZN-7, and qZN-11) associated with grain iron and zinc content. Out of 1063 potential simple sequence repeat (SSR) loci identified within these QTL regions, the researchers designed 22 novel SSR markers based on their proximity to genes related to metal transport. These markers were then validated for polymorphism in the parent rice lines.

3) Phenotypic and Genotypic Analysis

The study analyzed the phenotypic data, revealing a significant variation in grain micronutrient content among the 73 lines of the mapping population. The mean iron and zinc content ranged from 9.68 to 19.98 μg/g and 15.5 to 20.84 μg/g, respectively. The analysis of variance indicated significant differences in these traits. Through genotyping, it was found that the indica parent, Swarna, contributed more allele types in the population than the japonica parent, Moroberekan. Ultimately, 18 polymorphic SSR markers were linked to the QTLs governing micronutrient content.

4) Association Mapping and Genomic Insights

Using single marker analysis, the authors established significant associations between several SSR markers and both iron and zinc contents in rice grains. Markers such as gRMm33-3, gRMm34-1, and gRMm7-2 showed a strong correlation with these micronutrient traits, underscoring the importance of these SSRs in further breeding efforts. The research also highlighted the variability in expression of candidate genes linked to mineral transport, suggesting their roles in enhancing the micronutrient content of rice grains.

5) Conclusion

This study presents critical insights into the genetic mechanisms underlying micronutrient content in rice. By identifying and validating novel SSR markers linked to specific QTLs, the research contributes valuable tools for the development of biofortified rice varieties. The implications of these findings are significant for addressing micronutrient deficiencies in populations reliant on rice as a staple food, emphasizing the need for continued research in molecular breeding techniques to enhance the nutritional quality of this essential crop.

FAQ section (important questions/answers):

What was the focus of the study on rice grain content?

The study focused on identifying QTLs controlling grain iron, zinc, and protein content in rice. It aimed to use molecular markers for developing iron and zinc-rich rice varieties through marker-assisted breeding techniques.

What is the genetic basis for the traits studied in rice?

The traits, including grain iron, zinc content, and protein, are complex polygenic traits governed by multiple interacting QTLs. The study involved co-segregation analysis of a mapping population derived from parents Swarna and Moroberekan.

How were the novel SSR markers identified in the study?

A total of 1063 potential SSR markers were identified within the genomic regions of known QTLs. Out of these, 22 novel SSR markers were designed for further validation and experimental use.

What were the significant findings regarding grain micronutrient content?

The study found that grain iron content ranged from 9.68 to 19.98 µg/g, while zinc content ranged from 15.85 to 20.84 µg/g among the mapping population. This indicated genetic variation and potential for biofortification.

What methodology was used for statistical analysis in the research?

Data were analyzed using a randomized block design, with various parameters including standard deviation and coefficients of variation being calculated. ANOVA tests were performed to identify significant differences in grain nutrient content.

What practical applications can be derived from this research?

The research can aid in developing biofortified rice varieties to combat micronutrient malnutrition. Using identified SSR markers, breeders can enhance the iron and zinc content of rice, improving nutritional quality for populations reliant on rice as a staple food.

Glossary definitions and references:

Scientific and Ayurvedic Glossary list for “In silico characterization of QTLs for GPC and micronutrient content.”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.

1) Rice (Rce):
Rice, a staple food for over half of the world's population, plays a crucial role in global nutrition. It is essential in combating food insecurity, particularly in Asia. The study of rice varieties enriched with micronutrients like iron and zinc is vital for improving health in regions with prevalent malnutrition.

2) Table:
The term 'Table' often refers to organized data presented in rows and columns. In scientific research, tables outline experimental results, statistical analyses, or comparative data. This aids in visualizing and interpreting information, making it easier for researchers and readers to understand findings related to variables being studied.

3) Family:
In the context of biology and genetics, 'Family' refers to a taxonomic rank in the classification of organisms. Within this study, it can represent a group of related genes or traits. Understanding family relationships among genes can enhance the knowledge of inheritance patterns and functional characteristics.

4) Line:
In agricultural research, 'Line' refers to a specific breeding line or genetic lineage of plants cultivated for desirable traits. The study involves mapping populations composed of various lines that exhibit traits like micronutrient content, providing insights into genetic variation and breeding strategies for enhancement of nutritional qualities.

5) Study (Studying):
The word 'Study' designates a systematic investigation aimed at acquiring knowledge on a particular subject. This research examines the genetic and phenotypic traits of rice, focusing on micronutrient contents. Studies like these contribute significantly to advancements in agricultural biotechnology and improving food quality.

6) Developing:
Transformation within 'Developing' nations focuses on improving agricultural practices and food security. This research aims to address the nutritional needs of populations in these regions, where micronutrient deficiencies are prevalent, by developing rice varieties that are higher in iron and zinc through genetic discovery and breeding.

7) India:
India, a significant global rice producer, faces challenges regarding malnutrition, particularly among children and women. With large populations largely dependent on rice for sustenance, research aimed at biofortifying rice with essential nutrients is critical to improving public health and achieving nutritional goals in the country.

8) Malnutrition:
Malnutrition refers to a condition arising from insufficient, excessive, or imbalanced nutrient intake. It is a major public health issue, especially in developing countries. The research addresses malnutrition by exploring iron and zinc deficiencies in rice, aiming to enhance grain quality and nutritional status of consuming populations.

9) Channel:
In biological contexts, 'Channel' might refer to cellular structures that facilitate the movement of ions or nutrients across cell membranes. Understanding channel proteins involved in nutrient uptake in rice can support the development of varieties with enhanced abilities to absorb essential minerals, impacting overall nutrient content.

10) Seedling:
Seedling refers to a young plant developed from a seed. In this study, analyzing seedlings helps determine the growth patterns and nutrient uptake early in development. Understanding seedling characteristics assists in selecting rice varieties that can thrive and produce high micronutrient content for improved dietary benefits.

11) Mineral:
Minerals are inorganic nutrients essential for human health, playing significant roles in physiological functions. The focus on minerals like iron and zinc in rice research is pivotal for biofortification strategies, aimed at combating deficiencies in populations reliant on rice as a primary dietary component.

12) Food:
Food encompasses substances consumed for nutrition and energy. The study emphasizes the importance of enhancing the nutritional value of staple foods like rice, which is vital for sustaining large populations and combating micronutrient deficiencies, thus improving health outcomes, particularly in vulnerable groups.

13) Crop:
Crop refers to cultivated plants harvested for food, fiber, or other economic purposes. This research targets rice crops to enhance their micronutrient content, using techniques such as marker-assisted selection. Developing nutrient-rich crops is essential for global food security and addressing malnutrition crises.

14) Rich (Rch):
In this context, 'Rich' refers to a high concentration of essential nutrients, particularly concerning food products. Researchers aim to create rice varieties rich in micronutrients like iron and zinc, thereby improving nutritional profiles and health outcomes for populations dependent on rice for their dietary needs.

15) Pur:
Poor indicates insufficient or inadequate levels, particularly regarding nutritional content in staple foods. In the research, 'poor' quality rice refers to varieties lacking essential micronutrients, emphasizing the need for biofortification efforts to improve grain quality and combat malnutrition effectively among affected populations.

16) Biofortification (Bio-fortification):
Biofortification is the process of increasing the nutritional value of food crops through genetic modification or conventional breeding. The research focuses on enhancing rice with higher levels of iron and zinc, essential to prevent deficiencies, thereby contributing positively to global health initiatives and nutritional security.

17) Suffering:
Suffering relates to the hardships experienced by individuals due to lack of essential nutrients leading to health issues. The study acknowledges the suffering caused by malnutrition, particularly iron and zinc deficiencies, emphasizing the need for enhanced nutritional content in staple crops like rice to alleviate health burdens.

18) Quality:
Quality refers to the standard or grade of something, in this case, the nutrient content of rice. The research assesses the quality of rice varieties to improve their nutritional traits, ensuring the grains provide sufficient iron and zinc crucial for preventing deficiencies and enhancing dietary health.

19) Taga:
Taga might refer to a particular trait, factor, or a formal classification relevant to the discussion. In agricultural research, the specificity of traits such as nutrient content may be denoted by terms like 'Taga,' providing a framework for understanding genetic variations significant for enhancing crop traits.

20) Pasha (Pasa):
Pasa could signify a component or element connected to the methodologies involved in the study. Understanding the contribution of various genetic factors, perhaps identified as 'Pasa,' assists in elucidating the complex relationships affecting grain nutrition in rice and developing effective breeding strategies.

21) Human body:
The human body requires a range of nutrients for optimal health, including essential minerals like iron and zinc. This research seeks to enhance rice varieties to meet these nutritional needs, addressing deficiencies in populations where rice constitutes a primary food source and thereby improving health outcomes.

22) Discussion:
Discussion pertains to the analytical dialogue surrounding research findings. It allows researchers to interpret their results, compare with existing literature, and outline implications for public health and agricultural practices. In this study, discussion on the impact of nutrient-rich rice is vital for future breeding programs.

23) Pursuing:
Pursuing describes the effort to achieve specific goals or objectives. In this study, pursuing nutritional enhancement in rice through scientific research signifies the commitment to develop varieties that effectively contribute to alleviating malnutrition and improving health conditions in populations reliant on rice.

24) Falling:
Falling can imply a decline or decrease in conditions or health standards. In relation to nutrition, it may represent the deteriorating health outcomes linked to poor micronutrient intake. Understanding 'falling' health statistics due to deficiencies can redirect research efforts to improve food quality.

25) Krishi (Krsi):
Krishi, meaning agriculture in Hindi, emphasizes the significance of agricultural practices in economic and food security contexts. The research underscores the importance of improving agricultural techniques and breeding strategies in India, aiming to enhance the nutritional quality of rice, a key staple crop.

26) Animal:
Animal refers to living organisms that comprise a diverse group, including humans. In agricultural research, understanding the comparative nutrition needs of animals can parallel the significance of nutritional quality in crops like rice, reinforcing the importance of developing nutrient-rich food sources for both humans and livestock.

27) Farmer:
Farmers are the individuals engaged in agricultural production and cultivation. Their role is vital in implementing findings from research such as biofortification efforts. Equipping farmers with knowledge about nutrient-rich rice varieties will enhance food security and contribute to improved dietary health in their communities.

28) House:
House may denote a physical structure of living, but metaphorically in agricultural contexts, it may represent the food chain or community resources available for nutrition. Delivery of nutrient-rich food to households is essential for combating malnutrition and enhancing overall community health.

29) Cata:
Cata may refer to specific agricultural terms or processes related to the study. However, in this context, its precise meaning might require further clarification, perhaps linking to aspects of plant biology or genetic mapping that contribute to understanding nutrient traits in rice.

30) Diet:
Diet refers to the sum of food consumed by an individual, encompassing the nutritional aspect. The research aims to improve the diet of populations by enhancing rice varieties with crucial minerals, significantly contributing to overall health and mitigating deficiencies associated with poor dietary practices.

31) Hand:
Hand refers to the physical act of manual work or assistance. In agricultural contexts, 'hand' could symbolize the labor involved in food production or preparation processes. This emphasizes the importance of hands-on approaches in implementing research findings to boost food quality and nutrition.

32) Salt (Salty):
Salt, a common nutrient and essential for bodily functions, also represents an area of agricultural focus. In this study, addressing soil salinity issues and enhancing nutrient absorption in rice plays a crucial role in developing crops that can thrive in various challenges to improve overall quality.

33) Post:
Post usually denotes after or subsequent to an event. In the research context, post refers to the results or findings that follow experimental trials. Analyzing post-harvest data is essential to assess the effectiveness of breeding techniques in producing rice with improved nutritional content.

Other Science Concepts:

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Discover the significance of concepts within the article: ‘In silico characterization of QTLs for GPC and micronutrient content.’. Further sources in the context of Science might help you critically compare this page with similair documents:

Micronutrient malnutrition, Phenotypic Characterization, PCR analysis, Genotype, Candidate gene approach, Homologous recombination.

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