In vitro antioxidant activity of methanolic extract of callicarpa arborea leaves
Journal name: World Journal of Pharmaceutical Research
Original article title: In vitro antioxidant activity of methanolic extract of callicarpa arborea leaves
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M.C. Lallianchhunga, L. Inaotombi Devi C. Lalmuanthanga, C. Lalchhandama P.K. Subudhi and M. Ayub Ali
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World Journal of Pharmaceutical Research:
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Full text available for: In vitro antioxidant activity of methanolic extract of callicarpa arborea leaves
Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research
Doi: 10.20959/wjpr20164-6061
Copyright (license): WJPR: All rights reserved
Summary of article contents:
Introduction
The research article investigates the in vitro antioxidant activity of the methanolic extract of Callicarpa arborea leaves, commonly known as the Beautyberry Tree. This plant is part of the Verbenaceae family and is used in traditional medicine to treat various ailments, including jaundice, fever, and skin diseases. The study focused on evaluating the antioxidant potential of this plant material to understand its benefits in preventing oxidative stress-related degenerative diseases.
Antioxidant Activity of Callicarpa arborea
The antioxidant activity was assessed through three key methods: DPPH free radical scavenging activity, Ferric Reducing Antioxidant Potential (FRAP) assay, and total phenolic content estimation. The DPPH free radical scavenging method measures the ability of antioxidants to donate hydrogen, thereby neutralizing free radicals. The study found the antioxidant activity to be 9.78 ± 0.12 mg Trolox equivalent (TE) per gram of the dried leaves. This indicates that Callicarpa arborea possesses significant potential as a natural antioxidant source, confirming previous findings by other researchers.
Ferric Reducing Antioxidant Potential (FRAP) Assay
The FRAP assay measures the reducing power of antioxidants by evaluating the reduction of ferric ions (Fe³⁺) to ferrous ions (Fe²⁺). In this study, the FRAP activity was reported as 12.81 ± 0.64 mg TE per gram of dried leaves. This result indicates the presence of powerful electron-donating antioxidants in the methanolic extract of the leaves, thereby suggesting their ability to reduce oxidative stress effectively.
Total Phenolic Content
The evaluation of total phenolic content (TPC) is essential as phenolic compounds are known for their antioxidant properties. However, the study revealed that the total phenolic content in the Callicarpa arborea leaves was not detectable. This absence of phenolic compounds could indicate either low quantities present or that other non-phenolic antioxidant compounds might be responsible for the observed antioxidant activities. Similar findings were reported in other studies concerning different extracts of the same plant.
Conclusion
The findings of this research underscore the significant antioxidant activity of the methanolic extract of Callicarpa arborea leaves, demonstrating its potential role in preventing degenerative diseases related to oxidative stress. The study suggests that this plant could serve as a valuable source of natural antioxidants. Further research could explore the specific compounds responsible for its antioxidant properties, which may facilitate the development of therapies aimed at combating oxidative stress.
FAQ section (important questions/answers):
What is the scientific name of the Beautyberry Tree?
The scientific name of the Beautyberry Tree is Callicarpa arborea, which belongs to the Verbenaceae family and is recognized for its traditional medicinal uses.
What traditional uses does Callicarpa arborea have?
Callicarpa arborea is traditionally used for treating jaundice, fever, headache, stomachache, skin issues, and scorpion bites.
What methods were used to analyze antioxidant activity?
Three methods were used to analyze antioxidant activity: DPPH free radical scavenging, Ferric reducing antioxidant potential (FRAP) assay, and total phenolic content estimation.
What were the DPPH and FRAP antioxidant activity results?
The DPPH free radical scavenging activity was 9.78 mg Trolox equivalent/g, and the FRAP assay result was 12.81 mg Trolox equivalent/g of dry leaves.
Was any phenolic content detected in the leaves?
No detectable amount of total phenolic content was found in the methanolic extract of the Callicarpa arborea leaves, possibly due to low quantities.
What conclusion was drawn from the study on Callicarpa arborea?
The study concluded that the methanolic extract of Callicarpa arborea leaves possesses significant antioxidant activity, indicating potential for preventing degenerative diseases.
Glossary definitions and references:
Scientific and Ayurvedic Glossary list for “In vitro antioxidant activity of methanolic extract of callicarpa arborea leaves”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.
1) Activity:
In the context of this research, 'activity' refers to the functional capability of the methanolic extract of Callicarpa arborea leaves in exhibiting antioxidant properties. Antioxidant activity is crucial for neutralizing harmful free radicals, thus providing protective effects against oxidative stress that can lead to various health issues.
2) Disease:
The term 'disease' is relevant as the study investigates the potential medicinal properties of Callicarpa arborea leaves, used traditionally to treat ailments like jaundice, fever, and cancer. Understanding diseases is essential for evaluating the effectiveness of natural substances in addressing health challenges linked to oxidative damage.
3) Science (Scientific):
'Science' underpins the methodology and analysis in the study, which employs rigorous techniques to measure antioxidant activity. It emphasizes the systematic investigation and understanding of natural products, focusing on their biochemical properties, efficacy, and potential application in medicine, aligning with a broader scientific inquiry into health.
4) Veterinary science:
'Veterinary science' is relevant here as the research is conducted at the College of Veterinary Sciences, indicating the intersection between animal health and medicinal plant research. It highlights the potential use of herbal remedies in veterinary medicine, contributing to holistic approaches to animal treatment and welfare.
5) Evolution:
'Evolution' can be interpreted in the context of the ongoing development of medicinal practices and natural substances over time. The study reflects on how traditional uses of plants have evolved into formal scientific investigations, integrating ancient knowledge with modern scientific methods to explore health benefits.
6) Species:
In this research, 'species' refers to Callicarpa arborea, the plant being studied. Species classification helps in understanding the biological and ecological characteristics of the plant, which may influence its chemical properties and therapeutic potential. It underscores the importance of biodiversity in natural medicine.
7) Powder:
'Powder' is significant as it indicates the form in which the plant leaves were processed for extraction of antioxidants. The grinding of leaves into powder increases the surface area, enhancing the efficacy of solvent extraction methods, which is crucial for obtaining measurable antioxidant activities.
8) India:
'India' is relevant as the location where Callicarpa arborea was studied and traditionally used. The cultural context of India emphasizes the rich heritage of herbal medicine, highlighting the importance of regional plants and their applications in healthcare practices throughout Indian history.
9) Tree:
The term 'tree' describes the physical characteristics of Callicarpa arborea, a tall plant that is part of the Verbenaceae family. Understanding its botanical classification aids in exploring its ecological significance and potential environments where similar plants may thrive, informing conservation efforts.
10) Medicine:
'Medicine' is a central theme, emphasizing the research's focus on traditional healing practices. It underscores the exploration of natural compounds from plants like Callicarpa arborea for their therapeutic effects, contributing to the broader field of ethnopharmacology and the search for alternative treatments.
11) Scorpion:
'Scorpion' is mentioned as one of the ailments for which Callicarpa arborea is traditionally used, illustrating its relevance in folk medicine. This highlights the plant's significance in addressing local health issues, enhancing understanding of how traditional remedies are adapted to community needs.
12) Fever:
'Fever' represents one of the conditions treated with Callicarpa arborea, highlighting the plant's medicinal properties. The study's exploration of antioxidant activity may provide insights into its efficacy for fever management, reflecting the intertwined nature of plant use and disease treatment in traditional practices.
13) Cina:
Like India, 'China' is referenced as part of the geographical distribution of Callicarpa arborea. This reflects the broader Asian context of herbal medicine, reinforcing the need to understand regional plant use and the significance of cross-cultural medicinal applications in addressing health-related issues.
14) Devi:
'Devi' refers to one of the authors, signifying the collaborative effort behind the research. It underlines the importance of diverse contributions within scientific studies, enhancing the credibility of findings and fostering a culture of interdisciplinary collaboration across medical and scientific fields.
15) Inflammation:
'Inflammation' is relevant as a health condition associated with oxidative stress, justifying the investigation of antioxidant properties in Callicarpa arborea. Understanding its role in chronic diseases may lead to better strategies for utilizing natural compounds in managing inflammation, impacting overall health care.
16) Measurement:
'Measurement' refers to the quantitative assessment of antioxidant activity in the study. Precise and accurate measurements are critical in scientific research to validate findings and compare the efficacy of different extracts, directing further research into the therapeutic applications of natural products.
17) Manjunatha:
'Manjunatha' is mentioned as part of the methodology reference, indicating previous work that informed the extraction process. Citing established research enhances the study's credibility, linking it to a broader scientific discourse on herbal extraction methods and their effectiveness.
18) Discussion:
'Discussion' highlights the importance of interpreting findings in the context of existing literature. This section analyzes results, compares with prior studies, and explores implications, fostering a comprehensive understanding that contributes to advances in herbal medicine and scientific inquiry.
19) Container:
'Container' refers to the storage method for extracted materials, crucial for preserving bioactive compounds. Proper containment is important in maintaining the integrity of extracts before analysis, ensuring that the results obtained accurately reflect the antioxidant potential of the plant material.
20) Realising (Realizing):
'Realizing' pertains to the acknowledgment of the significance of studying plant antioxidants. It signifies the growing understanding of the role natural compounds can play in healthcare, encouraging further exploration that may enhance preventive medicine strategies and promote the use of natural resources.
21) Malaya:
'Malaya' is referenced in relation to the geographical range of Callicarpa arborea, demonstrating its wider distribution and use in traditional medicine across various cultures. Understanding such distribution is essential for conservation and utilization of plant species in different regions.
22) Muslin:
The term 'muslin' is noted as a filtration method used in the extraction process. Selecting appropriate materials for filtration is vital in scientific research as it affects the purity and concentration of the extracts obtained, directly influencing the study's outcomes.
23) Family:
'Family' relates to the botanical classification of Callicarpa arborea within Verbenaceae. This classification helps in understanding the characteristics shared among related plants, potentially guiding research into their collective medicinal properties and ecological roles.
24) Cancer:
'Cancer' is one of the diseases linked to oxidative stress that is of interest in the anti-cancer potential of herbal extracts. Investigating such properties emphasizes the importance of identifying natural compounds that can aid in cancer prevention and treatment strategies.
25) Animal:
'Animal' connects to the veterinary aspect of the research, indicating the target applications of the study's findings. It showcases the potential for using natural antioxidant sources to enhance animal health and nutrition, expanding the relevance of the research beyond human medicine.
26) Campu:
'Campu' refers to the 'campus' where the plant sample was collected, indicating the research setting. This highlights the importance of local biodiversity in academic research and the role of institutions in fostering studies that utilize native plant resources for health benefits.
27) Kumar:
'Kumar' is cited in reference to previous research, linking the current study to a broader scientific conversation on antioxidant properties and plant studies. Acknowledging past work helps contextualize findings and encourages ongoing exploration within the field.
28) Table:
'Table' is utilized to present results succinctly, illustrating the antioxidant activity of Callicarpa arborea. Tables are crucial in scientific writing for organizing data clearly, making it easier for readers to interpret results and draw conclusions based on empirical evidence.
Other Science Concepts:
Discover the significance of concepts within the article: ‘In vitro antioxidant activity of methanolic extract of callicarpa arborea leaves’. Further sources in the context of Science might help you critically compare this page with similair documents:
Traditional medicine, Antioxidant activity, Herbarium specimen, Reactive oxygen species, Total phenolic content, Analytical grade, DPPH free radical, Degenerative disease, Gallic acid, UV-visible spectrophotometer, Incubation time, Rotary evaporator, Sodium acetate, Phenolic compound, Cold maceration.