In vitro antioxidant potential of blepharis persica (burm f) o kuntze (seeds)
Journal name: World Journal of Pharmaceutical Research
Original article title: In vitro antioxidant potential of blepharis persica (burm f) o kuntze (seeds)
The WJPR includes peer-reviewed publications such as scientific research papers, reports, review articles, company news, thesis reports and case studies in areas of Biology, Pharmaceutical industries and Chemical technology while incorporating ancient fields of knowledge such combining Ayurveda with scientific data.
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Vichitra Kaushik, Gagandeep Chaudhary, Shoaib Ahmad and Vipin Saini
World Journal of Pharmaceutical Research:
(An ISO 9001:2015 Certified International Journal)
Full text available for: In vitro antioxidant potential of blepharis persica (burm f) o kuntze (seeds)
Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research
Doi: 10.20959/wjpr20164-6059
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Summary of article contents:
Introduction
In recent years, the relevance of antioxidants has garnered increasing attention due to their role in combating oxidative stress, which is linked to numerous human diseases. Antioxidants can significantly prevent or delay the oxidation of essential substrates by neutralizing free radicals. This study investigates the antioxidant potential of the seeds of Blepharis persica, evaluating both ethanolic (90%) and aqueous extracts for their effectiveness in scavenging free radicals and mitigating oxidative stress. The research tests various methods, including DPPH free radical scavenging, ferric reducing power (FRPA), and lipid peroxidation assays (TBARS), to provide a comprehensive overview of the antioxidant properties present in B. persica seeds.
Total Phenolic and Flavonoid Content
The study highlighted the significant presence of total phenolic and flavonoid compounds in the ethanolic (90%) and aqueous extracts of B. persica seeds. The ethanolic extract exhibited a total phenolic content of 2.05% w/w and total flavonoids of 8.234% w/w, while the aqueous extract contained 0.45% w/w phenolics and 1.375% w/w flavonoids. These compounds are known for their capability to act as effective antioxidants. The quantification was performed using standard curves of gallic acid for phenolics and rutin for flavonoids. The presence of these phytochemicals supports the potential health benefits of B. persica as a natural source of antioxidants.
Antioxidant Activity via DPPH and FRPA Assays
The antioxidant activity of B. persica seeds was assessed using DPPH and FRPA assays, establishing its ability to scavenge free radicals effectively. The results demonstrated that both ethanolic (90%) and aqueous extracts showed promising scavenging activity, with the ethanolic extract exhibiting a lower IC50 value (32.05 µg/ml), indicating stronger efficacy compared to the aqueous extract (45.35 µg/ml). In the FRPA assay, both extracts displayed increasing reducing power with concentration, further establishing their potential as antioxidants. Ascorbic acid was used as a standard, reinforcing the notion that the extracts can indeed reduce oxidative stress by neutralizing harmful free radicals in biological systems.
Lipid Peroxidation (TBARS) Inhibition
The study also delved into the effects of B. persica extracts on lipid peroxidation using the TBARS assay. Both ethanolic and aqueous extracts exhibited anti-lipid peroxidation activities, although they remained less effective than the standard butyl hydroxytoluene (BHT). The IC50 values indicated that the ethanolic extract (93.00 µg/ml) was more efficient in inhibiting lipid peroxidation than the aqueous extract (125.34 µg/ml). These findings suggest that B. persica seeds help prevent cellular damage by scavenging free radicals and inhibiting the lipid peroxidation process, thus supporting their use in traditional medicine for health maintenance.
Conclusion
The research findings unequivocally showcase the significant antioxidant activity of ethanolic (90%) and aqueous extracts of B. persica seeds. The presence of beneficial phenolic and flavonoid compounds, alongside effective free radical scavenging and lipid peroxidation prevention, positions B. persica as a valuable natural antioxidant source. Its potential applications in medicine and the food industry could leverage these antioxidant properties to combat oxidative stress and related diseases. However, further studies are warranted to identify specific bioactive compounds responsible for these observed effects, aiding in the development of targeted therapies based on traditional medicinal knowledge.
FAQ section (important questions/answers):
What was the focus of the study on Blepharis persica seeds?
The study investigated the in vitro antioxidant potential of ethanolic (90%) and aqueous extracts of Blepharis persica seeds, including their total phenolic and flavonoid contents.
How were the antioxidant activities measured in the study?
Antioxidant activities were measured using DPPH free radical scavenging, Ferric Reducing Power Assay (FRPA), and lipid peroxidation (TBARS) assays to assess free radical scavenging and reducing capacity.
Which extract showed better antioxidant potential in the study?
The ethanolic (90%) extract exhibited greater antioxidant potential compared to the aqueous extract, as demonstrated by its effectiveness in various antioxidant assays.
What is the source of antioxidants from Blepharis persica seeds?
The antioxidants in Blepharis persica seeds are attributed to phenolic compounds and flavonoids, which contribute to its ability to scavenge free radicals and reduce oxidative stress.
What traditional uses does Blepharis persica have in medicine?
Blepharis persica is traditionally used as a purgative, diuretic, tonic, and for treating disorders like urinary issues, liver disorders, and ascites.
What conclusions were drawn from the study on B. persica seeds?
The study concluded that both ethanolic (90%) and aqueous extracts of B. persica seeds possess significant antioxidant activities and could potentially be valuable in medical and food industries.
Glossary definitions and references:
Scientific and Ayurvedic Glossary list for “In vitro antioxidant potential of blepharis persica (burm f) o kuntze (seeds)”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.
1) Activity:
Activity refers to the effectiveness of the ethanolic and aqueous extracts of Blepharis persica in demonstrating antioxidant properties. In the study, the antioxidant activity was assessed through various assays, such as DPPH scavenging and FRPA, indicating the extracts' potential health benefits against oxidative stress-related damages.
2) Table:
Table is a structured representation of data within the study, providing summarized information on various measured parameters such as total phenolic and flavonoid content. Tables facilitate quick comparisons between different extracts or conditions, aiding in the clear presentation of experimental results and enhancing understanding.
3) Water:
Water serves as a solvent in the extraction process of the seeds of Blepharis persica. It is essential for preparing aqueous extracts, which were tested alongside ethanolic extracts for their antioxidant properties. Water's role in extraction highlights the importance of solvents in isolating bioactive compounds from plant materials.
4) Flavonoid:
Flavonoids are a group of polyphenolic compounds present in Blepharis persica, contributing to its antioxidant properties. The study measured flavonoid content in both ethanolic and aqueous extracts, indicating their potential role in scavenging free radicals and providing health benefits, such as reducing oxidative stress.
5) India:
India is the geographic region where Blepharis persica is indigenous. The study explored the use of this plant in traditional medicine, highlighting its significance in Indian herbal practices. The botanical, cultural, and nutritional context of India enhances the relevance of the antimicrobial and antioxidant findings.
6) Disease:
Disease refers to health conditions potentially mitigated by antioxidants. The study emphasizes the importance of the antioxidant properties of Blepharis persica in combating oxidative stress, which is linked to various diseases. This underscores the need for natural sources of antioxidants in disease prevention and management.
7) Medicine:
Medicine represents the broader context of therapeutic interventions, including the use of plant-derived extracts like Blepharis persica for health benefits. The findings suggest that the studied extracts may serve as natural medicinal sources, aligning with traditional uses in various healthcare systems, including Ayurveda and Unani.
8) Punjab:
Punjab is the specific region in India where the seeds of Blepharis persica were collected and studied. Understanding its local significance highlights the plant's traditional uses and its relevance within regional herbal medicine, agriculture, and ecology concerning its antioxidant potential and health applications.
9) Blood:
Blood is relevant in the context of the study as free radicals can cause oxidative damage to red blood cells, leading to conditions like thalassemia. The study's findings on antioxidant potential imply a protective role for Blepharis persica extracts in maintaining blood health and counteracting oxidative stress-related disorders.
10) Study (Studying):
Study denotes the systematic investigation conducted to explore the antioxidant potential of Blepharis persica seeds. The research encompassed various methodologies to determine the extracts' effectiveness, contributing valuable empirical data to the field of pharmacognosy and further research on natural antioxidants.
11) Powder:
Powder refers to the form in which the seeds of Blepharis persica were processed for extraction. The seeds were shade dried and coarsely powdered to increase the surface area for better solvent extraction of bioactive compounds, emphasizing the role of preparation methods in enhancing the effectiveness of herbal remedies.
12) Gupta:
Gupta likely refers to the authors or researchers who have previously studied or reported on the medicinal properties of Blepharis persica. Citing past research by Gupta lends credibility to the current study and links it to established scientific knowledge regarding the plant's therapeutic applications.
13) Food:
Food is essential in the discussion of dietary antioxidants, as the study implicates the potential use of Blepharis persica extracts in the food industry. Its antioxidant properties can be utilized to enhance food preservation and promote health, thus drawing a link between nutrition and natural compounds.
14) New Delhi:
New Delhi is where the plant samples were authenticated, reflecting its academic importance. The location provided a formal verification of the species studied, thereby ensuring accurate identification in research, which is critical for the reliability of results in pharmacognostic and medicinal studies.
15) Sunishannaka (Sunisannaka):
Sunishannaka is one of the traditional names for Blepharis persica, illustrating the cultural significance and historical use of the plant in traditional medicine practices. Such names often indicate the plant's observed effects or uses, contributing to its recognition in ethnobotany.
16) Thalassemia:
Thalassemia is a blood disorder mentioned in the context of oxidative stress and free radical damage. The study's results imply that the antioxidant properties of Blepharis persica may help reduce oxidative damage in patients with blood disorders like thalassemia, thus linking traditional herbal remedies to modern health issues.
17) Calculation:
Calculation involves the quantitative determination of antioxidant activity, total phenolic, and flavonoid content in the extracts. Accurate calculations are crucial for establishing the effectiveness and potential health benefits of Blepharis persica in combating oxidative stress and warrant further research in pharmacological applications.
18) Measurement:
Measurement pertains to the process of quantifying specific parameters like antioxidant capacity and total phenolic content in the study. Accurate measurement ensures the reliability of findings and comparisons, essential for validating the potential use of Blepharis persica in health-related applications.
19) Discussion:
Discussion refers to the analysis section of the study where results are interpreted in light of existing literature. This section synthesizes findings to highlight the implications of the antioxidant properties of Blepharis persica and suggests areas for future research within the field of natural products and pharmacology.
20) Science (Scientific):
Scientific highlights the nature of the study, which is grounded in empirical methods and biological investigations. The rigorous approach taken to assess the antioxidant properties reinforces the credibility of the findings, aligning them with broader scientific research aimed at uncovering the health benefits of botanical extracts.
21) Rajasthan:
Rajasthan is another geographic region in India where Blepharis persica is indigenous. Its presence highlights the ecological distribution of the plant and reinforces the relevance of regional studies that may uncover diverse traditional uses in local practices across varying climates and cultures.
22) Substance:
Substance refers to the bioactive compounds isolated from the extracts of Blepharis persica. Understanding the specific substances responsible for antioxidant activities is essential for evaluating their therapeutic potential and could lead to the development of natural antioxidants in both food and pharmaceutical industries.
23) Container:
Container signifies the storage method used for dried and powdered seeds of Blepharis persica. Proper storage in airtight containers is necessary to preserve the integrity and potency of the extracts until they are further processed and utilized in research or applications.
24) Species:
Species identifies the specific classification of Blepharis persica within the plant kingdom. Understanding the precise species is vital for accurately assessing its therapeutic potential and for further research focused on the ecological and pharmacological aspects of related species.
25) Madhya:
Madhya refers to the Madhya Pradesh region in India, which is part of the native habitat for Blepharis persica. Its inclusion emphasizes the plant's ecological relevance and the importance of understanding regional biodiversity and traditional knowledge regarding medicinal plants throughout different geographical areas.
26) Sharman (Sarma, Sharma, Sarman):
Sharma potentially references research conducted by the named author, indicating past contributions to the field. Citing previous studies by Sharma enriches the academic context of the current research and draws connections to established findings regarding the medicinal properties of plants.
27) Delhi:
Delhi is mentioned as a significant location for the authentication and validation of Blepharis persica plant material. As the capital city of India, it serves as a central hub for scientific research, providing important resources and institutions for the verification of botanical studies.
28) Malwa:
Malwa is a region in Madhya Pradesh, India, where Blepharis persica is found. Mentioning Malwa adds to the geographical diversity and traditional uses of the plant, reinforcing its role within local cultural contexts and indigenous medical practices, connecting history to contemporary research.
29) Pandu (Pamdu, Pamde):
Pande likely refers to a researcher or author whose work on Blepharis persica adds to the current understanding of its medicinal uses. Citing Pande highlights the cumulative knowledge surrounding the plant and its applications, supporting the importance of collaborative research in ethnobotany.
30) Drug:
Drug signifies the therapeutic applications of extracts from Blepharis persica in traditional medicine. This term encapsulates the potential of the plant's antioxidant properties to contribute to drug development, especially in managing oxidative stress-related diseases and promoting health in conventional and alternative medicine.
31) Tar:
Thar refers to the desert region, which is part of the indigenous habitat for Blepharis persica. Its association with arid climates highlights the plant's resilience and adaptability, providing insights into the ecology and ethnobotanical practices related to medicinal plants in challenging environments.
32) Male:
Male signifies the biological context within the experiment involving male rats for the bud peroxidation assays. Understanding the specific subject population is critical for generalizing the research findings, ensuring clarity about potential variable impacts pertaining to different biological sexes in pharmacological studies.
Other Science Concepts:
Discover the significance of concepts within the article: ‘In vitro antioxidant potential of blepharis persica (burm f) o kuntze (seeds)’. Further sources in the context of Science might help you critically compare this page with similair documents:
Oxidative stress, Reactive oxygen species, Free radical scavenging activity, Aqueous extract, Ethanolic extract, Total phenolic content, Total flavonoids content, Bioactive compound, Lipid peroxidation assay, Traditional formulation.