GC-MS analysis of bioactive compounds in Loeseneriella arnottiana.

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Journal name: World Journal of Pharmaceutical Research
Original article title: Identification of bioactive compounds in loeseneriella arnottiana wight root by gc-ms analysis
The WJPR includes peer-reviewed publications such as scientific research papers, reports, review articles, company news, thesis reports and case studies in areas of Biology, Pharmaceutical industries and Chemical technology while incorporating ancient fields of knowledge such combining Ayurveda with scientific data.
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Original source:

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Author:

Prajna P.S., Rama Bhat P. and Sunil Kumar K. N.


World Journal of Pharmaceutical Research:

(An ISO 9001:2015 Certified International Journal)

Full text available for: Identification of bioactive compounds in loeseneriella arnottiana wight root by gc-ms analysis

Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research

Doi: 10.20959/wjpr20164-6013

Copyright (license): WJPR: All rights reserved


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Summary of article contents:

Introduction

Loeseneriella arnottiana, a climbing shrub belonging to the family Celastraceae, is well-regarded in folklore medicine for its potential therapeutic properties, particularly in the treatment of diabetes mellitus. The plant is prevalent in regions of South Canara, Malabar, and Travancore. Initial phytochemical analyses of its root extract highlighted the presence of various compounds including tannins, flavonoids, saponins, glycosides, terpenoids, and alkaloids. The study detailed herein employs Gas Chromatography-Mass Spectroscopy (GC-MS) to identify the bioactive substances in the n-hexane extract of L. arnottiana root.

Bioactive Compounds Identified

GC-MS analysis revealed the presence of 40 low polar constituents in the n-hexane extract. Key identified compounds included beta-amyrin (14.92%), squalene (9.97%), and alpha-amyrin (9.14%), which were recognized as major constituents due to their substantial abundance. The analysis illustrated that beta-amyrin and alpha-amyrin are triterpenes, known for showing various biological activities, including anti-inflammatory and antimicrobial effects. Squalene, another significant compound, also serves as a natural antioxidant, highlighting its role in health-promoting properties.

Pharmacological Activities of Amyrins

Both alpha- and beta-amyrins have been documented to exhibit multiple pharmacological activities. These triterpenes show potentials such as anti-inflammatory, antimicrobial, and antihyperglycemic activities, making them valuable for treating conditions like diabetes and infections. Research has indicated that these compounds can significantly reduce high blood glucose and cholesterol levels, which are critical in metabolic disorders. Their presence in L. arnottiana reinforces the plant's application in traditional medicine, suggesting it may act as a natural adjuvant for enhanced therapeutic outcomes in various health conditions.

Health Benefits of Squalene

Squalene, a prominent constituent in L. arnottiana, plays a crucial role in human health through its function in cholesterol synthesis and its dietary significance. As an antioxidant and immunostimulant, squalene helps in shielding the body from oxidative stress and possible carcinogenic effects. Furthermore, its application in skin care due to its role in maintaining skin health has been widely explored. Studies emphasized that dietary squalene could contribute to preventing cardiovascular diseases by regulating cholesterol levels, showcasing its potential as an integrated health supplement.

Conclusion

The research on the n-hexane extract of L. arnottiana highlights the plant's wealth of bioactive compounds that possess a variety of pharmacological properties, including antioxidant, anti-inflammatory, antidiabetic, and antimicrobial capabilities. These characteristics provide a scientific foundation for the traditional medicinal uses of L. arnottiana, supporting its role in holistic treatment approaches. The findings underscore the need for further investigations into the therapeutic applications of these compounds, which may lead to the development of novel drug therapies or health supplements derived from this promising plant.

FAQ section (important questions/answers):

What are the primary bioactive compounds found in L. arnottiana?

The primary bioactive compounds identified in L. arnottiana include beta-amyrin, squalene, and alpha-amyrin, which possess various medicinal properties.

What method was used to analyze the extract of L. arnottiana?

The extract of L. arnottiana was analyzed using Gas Chromatography coupled with Mass Spectroscopy (GC-MS) to identify its components.

What phytochemical constituents were found in L. arnottiana root extract?

The phytochemical analysis revealed the presence of tannins, flavonoids, saponins, glycosides, steroids, terpenoids, and alkaloids in the root extract.

What potential therapeutic effects does L. arnottiana have?

L. arnottiana possesses anti-inflammatory, antidiabetic, antioxidant, anticancer, and antimicrobial properties, making it valuable for treating various diseases.

Where was the L. arnottiana plant material collected?

The roots of L. arnottiana were collected from Bantamale Reserve Forest, Sullia taluk, Karnataka, India.

What is the significance of the phytochemicals from L. arnottiana?

The active constituents of L. arnottiana are linked to human health, potentially offering therapeutic benefits for various conditions.

Glossary definitions and references:

Scientific and Ayurvedic Glossary list for “GC-MS analysis of bioactive compounds in Loeseneriella arnottiana.”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.

1) Activity:
The term 'Activity' refers to the various biological effects that compounds exhibit, especially in the context of medicinal plants like Loeseneriella arnottiana. Understanding the activity of bioactive compounds is essential for developing therapeutic applications in treating diseases such as diabetes, cancer, and inflammation. Each identified component's activity is crucial for evaluating its potential health benefits.

2) Phytochemical:
Phytochemicals are bioactive compounds naturally occurring in plants that contribute to their medicinal properties. They can have a range of effects on human health, including antioxidant, anti-inflammatory, and antimicrobial activity. Identifying and studying phytochemicals helps researchers understand the therapeutic potential of various plants and supports traditional medicine practices.

3) Beta:
Beta, in the context of this research, primarily refers to beta-amyrin, a triterpene compound identified in Loeseneriella arnottiana. Beta-amyrin is noted for its anti-inflammatory and antidiabetic properties, making it a significant focus for its potential health benefits and therapeutic applications in the context of managing chronic diseases.

4) India:
India is the geographical origin of Loeseneriella arnottiana and is known for its rich biodiversity, especially in medicinal plants used in traditional agriculture and healing practices. The research emphasizes the plant’s local application in folklore medicine, showcasing India’s cultural reliance on botanical resources for healthcare.

5) Table:
In scientific literature, 'Table' typically refers to a structured presentation of data or findings. In this study, various tables are used to summarize the results of phytochemical screening and GC-MS analyses, providing an organized overview of the identified compounds, their abundance, and associated biological activities.

6) Pharmacological:
Pharmacological pertains to the branch of medicine and biology concerned with the study of drug action and the effects of chemical compounds on biological systems. This term is essential in discussing the medicinal properties of the identified compounds and their potential for therapeutic use in various diseases and conditions.

7) Antibiotic (Antibacterial):
Antibacterial refers to the ability of a substance to inhibit or kill bacterial growth. Several compounds identified from Loeseneriella arnottiana demonstrate antibacterial activity, highlighting the plant's potential as a natural source of antimicrobial agents, which could be used to develop new drugs or treatments against bacterial infections.

8) Disease:
Disease refers to any deviation from normal physiological function that disrupts an individual's health. The research on Loeseneriella arnottiana focuses on its use in treating conditions like diabetes and the identification of bioactive compounds that may help combat various diseases, emphasizing the importance of medicinal plants in healthcare.

9) Karnataka:
Karnataka is a state in India where Loeseneriella arnottiana is found, highlighting the specific geographical context of this research. The state's rich biodiversity and traditional practices make it a significant area for studying medicinal plants and their applications in local and traditional medicine.

10) Flavonoid:
Flavonoids are a class of phytochemicals known for their antioxidant, anti-inflammatory, and potential health benefits. Their presence in plant extracts like Loeseneriella arnottiana emphasizes the importance of studying such compounds for their contributions to the overall therapeutic value of medicinal plants.

11) Medicine:
Medicine encompasses the science and practice of diagnosing, treating, and preventing diseases. The research on Loeseneriella arnottiana serves to validate traditional medicinal practices through scientific inquiry, bridging the gap between folklore and modern pharmacology by identifying bioactive compounds with tangible therapeutic effects.

12) Species:
The term species denotes a group of organisms that can interbreed and produce fertile offspring. In this study, Loeseneriella arnottiana is the specific species investigated, underlining the importance of studying individual species for their unique medicinal properties and contributions to biodiversity.

13) Family:
In biological classification, the family groups related species exhibiting common characteristics. Loeseneriella arnottiana belongs to the Celastraceae family, which includes many other plants with potential medicinal uses. Understanding family associations aids in predicting compound presence and biological activity.

14) Water:
Water is crucial as a solvent and medium for extraction processes in phytochemical analysis. Understanding the role of water in the extraction of bioactive compounds from plants reinforces its importance in preparing herbal medicines and analyzing the phytochemical profiles of medicinal plants.

15) Human body:
The human body is the biological recipient of the compounds studied in medicinal research. Understanding how bioactive compounds from plants like Loeseneriella arnottiana affect human physiology and health is fundamental to developing effective treatments for diseases, emphasizing the connection between phytochemistry and health.

16) Inflammation:
Inflammation is a biological response to harmful stimuli, often linked to various diseases including diabetes and cancer. The study of Loeseneriella arnottiana aims to identify compounds that exhibit anti-inflammatory properties, thus contributing to the management and treatment of inflammatory conditions.

17) Surface:
Surface often refers to the outer layer of biological tissues or materials. In the context of squalene, a compound noted in the research, its role is vital in maintaining skin health and protecting against oxidative damage, illustrating the importance of understanding surface interactions in medicinal applications.

18) Cancer:
Cancer is a group of diseases characterized by uncontrolled cell growth. The identification of anticancer compounds in Loeseneriella arnottiana suggests its potential role in cancer treatment. Research aims to explore the mechanisms through which these compounds may inhibit cancer cell proliferation.

19) Nature:
Nature refers to the inherent qualities and biochemical diversity of living organisms. The study of the natural phytochemical constituents of Loeseneriella arnottiana illuminates the therapeutic possibilities embedded within nature's biodiversity, reinforcing the significance of natural products in advancing healthcare solutions.

20) Line:
Line in this context may refer to the lineage or the specific pathway related to biological compounds such as the metabolic pathways involving bioactive constituents. Understanding lineages helps in the study of compound interactions and their effects on human health.

21) Post:
Post may refer to the publication or communication of the study results. Sharing findings in peer-reviewed platforms helps disseminate knowledge about the medicinal properties of plants such as Loeseneriella arnottiana, contributing to broader recognition and application in pharmacological contexts.

22) Arrangement:
Arrangement pertains to how data or biological components are organized. In pharmacological studies, the arrangement of compounds within tables or figures helps simplify complex information, aiding in the clear presentation of results and facilitating understanding of the relationships between bioactive compounds.

23) Measurement:
Measurement refers to the process of obtaining data regarding the quantity or degree of specific compounds. Accurate measurement is crucial in GC-MS analysis to ascertain the prevalence of bioactive components, contributing to the reliability of the study's findings and their implications for health and medicine.

24) Discussion:
Discussion is a critical component of scientific research that interprets the findings and contextualizes them within existing literature. It allows researchers to articulate the significance of their results, propose practical applications, and identify areas for future study regarding the compounds identified in Loeseneriella arnottiana.

25) Similarity:
Similarity refers to the degree of likeness between different compounds or biological activities. In GC-MS analysis, identifying the similarity of mass spectra to established databases helps confirm the presence of specific bioactive compounds, supporting the validity of the research outcomes.

26) Ayurveda (Ayus-veda):
Ayurveda is a traditional system of medicine that employs plants and natural therapies for healing. The study of Loeseneriella arnottiana highlights its place in Ayurvedic practices, reaffirming the relevance of ancient knowledge in modern scientific research to explore and validate the therapeutic potential of traditional remedies.

27) Science (Scientific):
Science encompasses the systematic study of the structure and behavior of the physical and natural world through observation and experimentation. The research presented on Loeseneriella arnottiana exemplifies the scientific method, reinforcing the importance of empirical evidence in understanding medicinal plants and their effects.

28) Forest:
Forest refers to the natural habitat where Loeseneriella arnottiana is found. The ecological context of forests highlights the biodiversity of plant species crucial for natural medicine. Preservation of forest ecosystems is essential for maintaining this diversity and ensuring access to medicinal plants.

29) Animal:
Animal refers to living organisms that can benefit from the bioactive compounds derived from plants. Animal studies are often conducted to evaluate the efficacy and safety of medicinal compounds discovered in plants like Loeseneriella arnottiana, linking botanical research directly to health outcomes.

30) Kumar:
Kumar is a common surname in India, and in the context of this research, it refers to one of the authors involved in the study. The inclusion of multiple authors emphasizes collaborative scientific efforts and the collective expertise necessary to conduct comprehensive research in the field.

31) Ulcer:
Ulcer refers to a sore on the skin or mucous membrane that results from the loss of tissue. Some compounds identified in the study may possess properties that could be beneficial in treating ulcers, highlighting the significance of plant-derived substances in therapeutic applications for various ailments.

32) Blood:
Blood is a vital bodily fluid responsible for transporting nutrients and oxygen to cells. The study of compounds like beta-amyrin and their potential effects on blood glucose levels underscores the importance of understanding how natural products can influence metabolic health and address conditions like diabetes.

33) Study (Studying):
Study refers to the systematic investigation of a subject, in this case, the analysis of Loeseneriella arnottiana. The final findings contribute to the broader understanding of the plant's medicinal properties, demonstrating the importance of scientific inquiry in discovering new treatments and improving health outcomes.

34) Rama:
Rama is a common name in India, and in this context, it refers to one of the authors of the study. The inclusion of multiple researchers indicates a collaborative approach to the study, emphasizing the importance of teamwork in conducting comprehensive scientific investigations.

35) Ghat (Gham):
Ghat refers to the mountain ranges in Southwestern India, renowned for their biodiversity. The ecology of such regions supports the growth of many medicinal plants, including Loeseneriella arnottiana, underscoring the importance of geographical context in understanding the availability and utility of herbal medicines.

36) Tree:
Tree often represents larger perennial plants that play a pivotal role in the ecosystem. The study of Loeseneriella arnottiana serves to illustrate the importance of plant diversity, including trees and shrubs, in providing natural remedies and contributing to ecological health and sustainability.

37) Diet:
Diet refers to the consuming habits that impact health, particularly related to the intake of various nutrients. Understanding how the compounds identified in Loeseneriella arnottiana can integrate into human diets aids in developing nutritional recommendations aimed at improving health and preventing diseases.

38) Rich (Rch):
Rich signifies abundance in a particular context. The term here refers to the rich diversity of phytochemicals found in Loeseneriella arnottiana that contribute to its medicinal properties, highlighting the importance of biodiversity in medicine and promoting sustainable harvesting of medicinal plants.

39) Drug:
Drug refers to any substance used to diagnose, cure, treat, or prevent diseases. The research on Loeseneriella arnottiana explores the potential of identified bioactive compounds to develop new drugs, reaffirming the significance of natural products in modern pharmaceutical applications and drug discovery.

Other Science Concepts:

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Discover the significance of concepts within the article: ‘GC-MS analysis of bioactive compounds in Loeseneriella arnottiana.’. Further sources in the context of Science might help you critically compare this page with similair documents:

Pharmacological properties, Tannins and flavonoids, Antioxidant properties, Antimicrobial properties, Anti-inflammatory effect, Secondary metabolite, Biological activities, Anticancer properties, Preliminary phytochemical analysis, Alkaloid, GC-MS analysis, Squalene, N-hexane extract, Phytoconstituent.

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