Identification of co-localized ESTs in rice candidate genes.

| Posted in: Health Sciences Science

Journal name: World Journal of Pharmaceutical Research
Original article title: Identification and characterization of co-localized expressed sequence tags (ests) underlying putative candidate genes in rice (oryza sativa l.)
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Full text available for: Identification and characterization of co-localized expressed sequence tags (ests) underlying putative candidate genes in rice (oryza sativa l.)

Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research

Copyright (license): WJPR: All rights reserved


Summary of article contents:

Introduction

Rice (Oryza sativa) is a crucial staple food for over half of the world's population, particularly among impoverished communities in Asia and Latin America. With an increasing prevalence of micronutrient deficiencies, particularly iron (Fe) and zinc (Zn), enhancing the nutritional quality of rice has become imperative. The research investigates the genetic basis for grain micronutrient content and protein levels in rice and identifies quantifiable trait loci (QTLs) associated with these traits through marker-assisted breeding techniques.

Identification of QTLs for Micronutrient Content

The study targeted the identification of QTLs influencing iron and zinc content in rice grains, emphasizing the importance of genetic variability in breeding programs. A mapping population, derived from a cross between rice cultivars Swarna and Moroberekan, exhibited diverse ranges of grain micronutrient content. Specifically, the grain iron content varied from 9.68 to 19.98 μg/g, averaging 16.87 μg/g, while the zinc content ranged from 15.5 to 20.84 μg/g, averaging 25.76 μg/g. The analysis also identified five significant QTLs associated with these micronutrients, indicating their potential role in improving nutrient density in rice through genetic selection.

Development and Validation of SSR Markers

The research focused on developing novel Simple Sequence Repeat (SSR) markers derived from the genomic regions of identified QTLs related to grain micronutrient content. Out of a total of 1063 putative SSRs identified, 22 novel SSR markers were designed and tested for polymorphism in the parent lines. The study highlighted that these markers could aid in fine mapping of regions associated with micronutrient traits and facilitate the introgression of desired traits into elite rice varieties. The association analysis of these markers revealed strong correlations with both iron and zinc contents, further supporting their utility in breeding programs.

Genetic Analysis and Segregation Patterns

Genetic analysis of the mapping population showed significant segregation patterns, with the indica parent Swarna contributing disproportionately to the allelic pool compared to the japonica parent Moroberekan. The allelic segregation indicated that Swarna contributed approximately 60.6% and Moroberekan 34.32% to the overall allele frequency. This deviation from the expected 1:1 ratio underscores the complex inheritance patterns governing micronutrient traits in rice. Notably, SSR markers were significantly associated with grain micronutrient content, enhancing our understanding of the genetic foundations of these traits.

Conclusion

This research illustrates the profound genetic diversity present in rice and the potential for utilizing this diversity to enhance grain micronutrient content through breeding. The identification and characterization of QTLs and associated SSR markers provide a roadmap for developing nutrient-rich rice varieties. Such advancements hold promise not only for improving human nutrition and combating micronutrient deficiencies but also for addressing global food security challenges. The study paves the way for future research aimed at incorporating these markers into breeding programs to produce rice varieties with enhanced nutritional profiles.

FAQ section (important questions/answers):

What is the significance of grain micronutrient contents in rice?

Grain micronutrient contents, such as iron and zinc, are crucial for preventing malnutrition, particularly in developing countries where rice is a staple food. Enhancing these micronutrients can improve overall health and nutrition among populations dependent on rice.

What traits were analyzed in the rice mapping population?

The study analyzed traits including grain iron and zinc content, grain protein content, and the polymorphism of specific molecular markers. These traits help in understanding genetic variability and developing nutrient-rich rice through marker-assisted selection.

How were novel SSR markers identified and validated?

Novel SSR markers were identified using in silico tools from five known QTL regions. Out of 1063 SSR loci, 22 Class I markers were designed and validated in rice parents for their polymorphism in the mapping population.

What were the average iron and zinc contents in the rice lines?

The average grain iron content in the mapping population was 16.87 μg/g, while the average zinc content was 25.76 μg/g. These values indicate the variability among selected rice lines for these important micronutrients.

What impact does poor protein content in rice have on health?

Poor protein content in rice can lead to widespread protein malnutrition, especially in developing nations. This condition affects children's growth and health, contributing to higher rates of malnutrition-related diseases and impacting overall food security.

What is biofortification in the context of rice cultivation?

Biofortification involves enhancing the nutritional value of rice through genetic selection or agronomic interventions to increase micronutrient levels like iron and zinc in the edible grains, thereby combating micronutrient deficiencies in populations reliant on rice.

Glossary definitions and references:

Scientific and Ayurvedic Glossary list for “Identification of co-localized ESTs in rice candidate genes.”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.

1) Rice (Rce):
Rice is a vital staple food cultivated in approximately 90 countries and serves as a primary source of calories for over half of the global population. Its importance is particularly pronounced in Asia and Latin America, where it nourishes millions and is integral to the diet of the impoverished.

2) Table:
In the context of this study, a table serves as a critical tool for presenting quantitative data regarding grain micronutrient concentrations in rice. It allows readers to quickly assess and compare the nutritional content of different rice lines and cultivars, facilitating better understanding and decision-making.

3) Family:
Family refers to the concept of relatedness among species in biological classification. In this study, it may relate to the grouping of rice types or varieties, emphasizing the genetic relationships that impact traits such as micronutrient content, essential for developing nutrient-rich rice varieties.

4) Line:
In plant breeding, a 'line' refers to a group of plants derived from a single genetic lineage. The research involves analyzing different lines of rice to identify genetic variability in micronutrient content, which is crucial for breeding programs aimed at enhancing nutrition in rice populations.

5) India:
India is a significant player in rice cultivation, being one of the largest producers of rice globally. The country faces challenges related to malnutrition, making the enhancement of rice with micronutrients crucial to improving the nutritional status of its population, particularly among children.

6) Study (Studying):
The study involves the identification and characterization of genetic traits within rice that relate to micronutrient content. Through co-segregation analysis and QTL mapping, the research aims to enhance the nutritional value of rice, which is essential for addressing global malnutrition challenges.

7) Malnutrition:
Malnutrition refers to the deficiency or imbalance in the intake of nutrients necessary for health. The study highlights how rice is a poor source of essential micronutrients like iron and zinc, contributing to micronutrient deficiencies, particularly in developing countries, indicating the need for biofortification.

8) Channel:
In a biological or agricultural context, 'channel' may refer to the transport mechanisms for nutrients within plants. Understanding these pathways is essential for increasing nutrient uptake in rice, thus improving the grain's nutrient content as part of the research focus on enhancing rice quality.

9) Developing:
This term relates to nations that are in the process of economic growth and improvement in living standards, particularly in Asia and Africa. The study emphasizes the role of biofortification in developing countries, where enhancing rice nutrient content is vital for overcoming malnutrition.

10) Food:
Food encompasses substances consumed to provide nutritional support for the body. Rice is a staple food that contributes significantly to dietary energy. Enhancing its micronutrient content is a key focus of this research, as better nutrition is vital for food security and public health.

11) Crop:
In agriculture, crops like rice are cultivated for food production. The study focuses on the genetic improvement of rice as a crop, specifically targeting the development of varieties that are rich in micronutrients, which could play a crucial role in addressing nutritional deficiencies.

12) Rich (Rch):
In the context of nutrition, 'rich' refers to the high concentration of essential nutrients within food. The research aims to develop rice varieties that are rich in iron and zinc, thereby improving the nutritional quality of rice consumed, particularly in micronutrient-deficient populations.

13) Pur:
This word highlights the nutritional inadequacies in certain populations, particularly in developing countries. The study addresses issues related to poor grain micronutrient content in rice and aims to enhance it through genetic improvements, thereby addressing food-related health challenges faced by these populations.

14) Suffering:
Suffering pertains to the adverse health effects resulting from poor nutrition, such as malnutrition. This research underlines the importance of addressing the nutritional deficiencies in rice to mitigate health issues and improve quality of life for populations, particularly children in developing nations.

15) Mineral:
Minerals are essential nutrients that play critical roles in bodily functions. The focus of this study is on micronutrients, particularly iron and zinc, which often lack adequate levels in staple foods like rice, thereby necessitating efforts to biofortify rice to combat mineral deficiencies.

16) Taga:
Taga does not have a clear context from the provided text; it may refer to a specific term, location, or symbolic entity. However, if it refers to a part of rice cultivation or research, it could be tied to enhancing agricultural techniques or crop management practices.

17) Human body:
The human body requires various nutrients for health and proper functioning. This study emphasizes the need for food that supplies sufficient vitamins and minerals, pointing out the role of fortified rice as a potential solution to combat deficiencies affecting billions.

18) Biofortification (Bio-fortification):
Biofortification refers to the process of enhancing the nutritional quality of crops through breeding or agronomic practices. This research highlights its importance in developing rice varieties rich in essential nutrients like iron and zinc, aimed at improving nutrition in populations with malnutrition.

19) Seedling:
A seedling is a young plant that has developed from a seed. In the context of the research, studying seedlings is essential for understanding the early growth stages of rice plants and how genetic traits for nutrient uptake manifest, which impacts the overall grain quality.

20) Quality:
Quality in this study relates to the nutritional value of rice grains, particularly regarding their iron and zinc content. Improving the quality of rice is critical for addressing malnutrition in rice-eating populations, emphasizing the need for research and breeding efforts targeting these traits.

21) Falling:
Falling can refer to trends or states of decline, such as the decreasing micronutrient levels in widely consumed staple foods. In the context of this study, it highlights the urgency of addressing these declines through breeding programs to prevent further health impacts.

22) Animal:
In research contexts, animals may serve as model organisms or components of agricultural ecosystems. While the primary focus of this study is on crops, the linkage of animal nutrition with plant-based diets underlines the interdependence of agriculture and health for all species in food chains.

23) Farmer:
Farmers are individuals engaged in agriculture, crucial for the production of crops like rice. This study is significant for farmers as it explores ways to enhance crop quality, enabling them to contribute effectively to food security and improve income through better yielding varieties.

24) House:
In this context, 'house' could metaphorically represent households that rely on rice as a food source. The nutrition provided by rice affects household well-being, especially in regions where rice is a staple ingredient, underscoring the need for fortified varieties in those contexts.

25) Cata:
Cata appears as a fragment and may refer to a genetic aspect or methodology within the broader research context. Its specific implication may involve examination of genetic traits related to micronutrient uptake or composition in crops during research.

26) Diet:
A diet encompasses all food and drink consumed by an individual or population. The study stresses the importance of enhancing the dietary quality of staple foods, like rice, to improve health outcomes in populations with inadequate nutrition due to limited micronutrient intake.

27) Hand:
In the agricultural context, 'hand' may refer to manual methods involved in crop management and processing. Techniques employed by farmers in handling and cultivating rice are pivotal in ensuring the quality of grain produced and can impact nutrient levels.

28) Post:
Post may refer to the post-harvest processes involved in rice production, including storage and processing. These procedures are vital to maintaining the nutritional quality of rice and can significantly affect the availability of micronutrients for consumers.

Other Science Concepts:

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Discover the significance of concepts within the article: ‘Identification of co-localized ESTs in rice candidate genes.’. Further sources in the context of Science might help you critically compare this page with similair documents:

Total protein content, Protein energy malnutrition, Molecular marker, Genetic variability, Genomic region, Polymorphic marker.

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