Mapping candidate genes for grain iron and zinc in rice.
Journal name: World Journal of Pharmaceutical Research
Original article title: Mapping and characterization of putative candidate genes for grain iron and zinc content in rice by mpss signature analysis
The WJPR includes peer-reviewed publications such as scientific research papers, reports, review articles, company news, thesis reports and case studies in areas of Biology, Pharmaceutical industries and Chemical technology while incorporating ancient fields of knowledge such combining Ayurveda with scientific data.
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R. K. Zote, R. N. Dhawale, G. Chandel, S. B. Verulkar3 and A. S. Kotasthane
World Journal of Pharmaceutical Research:
(An ISO 9001:2015 Certified International Journal)
Full text available for: Mapping and characterization of putative candidate genes for grain iron and zinc content in rice by mpss signature analysis
Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research
Doi: 10.20959/wjpr20166-6424
Copyright (license): WJPR: All rights reserved
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Summary of article contents:
Introduction
Rice (Oryza sativa L.) is a fundamental staple food for over half of the world’s population, particularly in developing nations, where deficiencies in essential micronutrients such as iron (Fe) and zinc (Zn) are prevalent. This study follows the identification and characterization of candidate genes responsible for enhancing grain Fe and Zn content in rice through the analysis of quantitative trait loci (QTLs) using MPSS (massive parallel signature sequencing) signature analysis. Specific focus is placed on developing rice varieties enriched with these essential micronutrients through marker-assisted breeding techniques.
Mapping and Characterization of QTLs
The research involved mapping an F6 population derived from a cross between two rice cultivars, Swarna and Moroberekan. Co-segregation analysis revealed a significant variance in grain micronutrient content within the mapping population, with Fe content ranging from 9.68 to 19.98 μg/g and Zn content from 15.85 to 20.84 μg/g. By identifying five known QTLs (qFE-1, qFE-9, qZN-5, qZN-7, and qZN-11), the study utilized in-silico tools to discover 1063 novel simple sequence repeats (SSRs) in genomic regions associated with these QTLs. Primers were designed for 22 class I SSR markers for further validation.
Importance of Candidate Genes in Biofortification
A critical element of the study is the characterization of putative candidate genes within the identified QTLs that relate to grain micronutrient content. The research identified several genes including metal cation transporters and oxidoreductases that play roles in metal ion uptake and transport. By leveraging these candidate genes, the study proposes biofortification as a sustainable strategy for improving the nutritional quality of rice, thereby addressing the pressing issue of micronutrient malnutrition, commonly referred to as "hidden hunger" among populations reliant on rice as a primary food source.
Statistical Analysis and Genotypic Validation
The statistical analysis of the mapping population indicated significant genetic variation among the different breeding lines, allowing for an assessment of allele segregation. Among the novel SSR markers, a total of 18 displayed polymorphism, with significant deviation from the expected segregation ratios noted. Five selected polymorphic SSR markers demonstrated significant associations with both Fe and Zn contents in grain, indicating potential for these markers to be used in future breeding programs aimed at producing nutrient-rich rice varieties.
Conclusion
The study successfully mapped and characterized candidate genes associated with grain Fe and Zn content in rice, identifying valuable QTLs and novel SSR markers that can facilitate biofortification efforts. The results underscore the potential for genetic improvement of rice grain micronutrient content through molecular breeding techniques. The findings will contribute to breeding programs aimed at alleviating micronutrient deficiencies globally, thus enhancing food security and nutrition among vulnerable populations dependent on rice as a staple food.
FAQ section (important questions/answers):
What is the main focus of the study conducted by Zote et al.?
The study focuses on mapping and characterizing candidate genes for grain iron and zinc content in rice, specifically using marker-assisted breeding techniques to improve the nutritional value of rice.
What genetic analysis techniques were used in this research?
The research utilized co-segregation analysis, SSR marker identification, and association mapping techniques to study the genetic variability and association of SSR markers with grain iron and zinc contents.
What significant findings were revealed regarding iron and zinc contents?
The grain iron content ranged from 9.68 to 19.98 μg/g, while zinc content ranged from 15.85 to 20.84 μg/g among the studied rice lines, indicating variability in micronutrient content.
How many SSR loci were identified in the study?
A total of 1063 SSR loci were identified in the genomic regions of five known QTLs associated with grain iron and zinc content in rice.
What were the results of the parental polymorphism analysis?
The parental polymorphism analysis identified 18 polymorphic markers. The Allelic segregation analysis showed that the indica parent Swarna contributed approximately 60.6% of total amplified alleles.
What is biofortification in the context of this study?
Biofortification is a sustainable approach to enhance the nutritional value of crops, specifically focusing on improving iron and zinc content in edible portions of rice through agronomic practices or genetic selection.
Glossary definitions and references:
Scientific and Ayurvedic Glossary list for “Mapping candidate genes for grain iron and zinc in rice.”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.
1) Rice (Rce):
Rice is a staple food for more than half of the world's population, especially in Asia and Latin America. It serves as a primary source of calories and is crucial for food security. The study emphasizes enhancing rice's nutrient profile, specifically iron and zinc, to combat malnutrition.
2) Family:
In genetics, 'family' often refers to a classification of related organisms. In the context of rice, it is used to group various cultivars and species that share common traits. Understanding these relationships helps in identifying genes related to micronutrient content and improving breeding practices.
3) Table:
In scientific literature, tables are used to present data clearly and concisely. Here, tables are utilized to organize information on grain micronutrient contents, the results of statistical analyses, and the characterization of specific genes. This makes complex data more accessible and easier to interpret.
4) India:
India is one of the largest producers and consumers of rice. The context of the study highlights the significance of improving rice quality to address nutritional deficiencies prevalent in Indian populations, particularly among children. Enhancing micronutrient content in rice can significantly impact public health in India.
5) Line:
The term 'line' refers to genetic lines of plants that have been bred for specific traits. In the study, lines derived from the Swarna x Moroberekan cross are investigated for their micronutrient content. Identifying and characterizing these lines may lead to better rice varieties.
6) Study (Studying):
The study aims to map and characterize genes associated with iron and zinc content in rice. It employs molecular techniques to explore genetic variation and develop markers for breeding. Such research is crucial for addressing nutritional deficiencies and improving food quality through agricultural practices.
7) Malnutrition:
Malnutrition is primarily caused by insufficient intake of essential nutrients. This issue is particularly pressing in developing regions where diets lack key vitamins and minerals. The study addresses malnutrition by proposing biofortification strategies to enhance the micronutrient content of staple crops like rice.
8) Channel:
In the context of plant biology, 'channel' refers to proteins that facilitate the transport of ions and nutrients across cell membranes. Understanding these channels is essential for manipulating rice varieties to improve nutrient uptake, which is critical for increasing iron and zinc levels in rice grains.
9) Developing:
Developing nations often face significant challenges concerning food security and nutrition. In this study, the focus on agricultural improvement, particularly biofortification of rice, aims to improve health outcomes in populations suffering from micronutrient deficiencies prevalent in these regions.
10) Food:
Food is essential for human survival and health. This study highlights the importance of enhancing the nutritional quality of staple foods, such as rice, to combat deficiencies of micronutrients like iron and zinc, which are essential for growth and development.
11) Crop:
Crops are cultivated plants grown for food, fiber, or other purposes. The study concentrates on rice, a critical crop for global nutrition. By improving the nutritional profile of crops through genetic research, the study aims to address widespread micronutrient deficiencies.
12) Rich (Rch):
'Rich' in this context refers to crops that contain high levels of essential nutrients. The study emphasizes the need to develop rice varieties rich in iron and zinc to provide better nutritional options for populations suffering from malnutrition.
13) Pur:
Poor diet refers to a condition where the intake of essential nutrients is inadequate. Many populations, especially in developing countries, consume rice as a primary food source, which is often lacking in micronutrients. The study proposes strategies to convert rice into a more nutritious food option.
14) Suffering:
Suffering from malnutrition affects millions, especially in regions reliant on staple crops with low nutrient content. This study addresses the need for biofortification of rice, aiming to reduce the suffering caused by deficiencies of iron and zinc in rice-consuming populations.
15) Mineral:
Minerals are inorganic nutrients that are vital for human health. This study focuses on mineral content—specifically iron and zinc—in rice. Enhancing mineral levels in staple crops can significantly improve public health, especially in populations with high rates of mineral deficiencies.
16) Taga:
Though often appearing to be a typographical error or abbreviation, 'Taga' does not have a specific relevance in the context of the provided text. It may refer to a particular cultivar or research term. More context is needed to define its significance accurately.
17) Human body:
The human body requires various nutrients, including minerals like iron and zinc, for optimal health. The study highlights the importance of consuming foods rich in these nutrients to prevent deficiencies, particularly in populations that primarily consume rice as a staple.
18) Biofortification (Bio-fortification):
Biofortification is the process of enhancing the nutritional quality of food crops through agronomic practices or genetic engineering. This study advocates for biofortification of rice to increase its iron and zinc content, aiming to alleviate micronutrient deficiencies in developing countries.
19) Seedling:
Seedlings are young plants that develop from seeds. In this research, seedlings refer to the plants grown from the rice lines being studied for their micronutrient content. Their development stages are crucial for evaluating the genetic potential for enhanced nutritional traits.
20) Animal:
In agricultural research, animals can be related to studies of genetics and breeding or can serve as a comparison for plant traits. Understanding how animal nutrition parallels plant nutrition helps inform strategies for biofortification and breeding of nutrient-rich crops.
21) Farmer:
Farmers are crucial stakeholders in agriculture, responsible for cultivating crops that provide food security. This study is relevant to farmers as it seeks to enhance rice varieties, which can improve yields and nutritional quality, ultimately benefiting their livelihoods and communities.
22) House:
In agricultural contexts, 'house' may refer to greenhouses or controlled environments for growing plants. These environments are used in research studies to maintain optimal growing conditions for plants like rice, facilitating the investigation of micronutrient content in various conditions.
23) Cata:
Cata could refer to a specific methodology or a mineral that helps in plant growth; however, it appears to be out of place or a typographical error in this context. More detail is needed to determine its relevance accurately.
24) Diet:
A balanced diet is essential for good health, and it should include sufficient vitamins and minerals. The study focuses on enhancing the dietary value of rice through increased micronutrient content, addressing the needs of populations with poor nutritional intake.
25) Hand:
In the context of this research, 'hand' could refer to manual methods used in agriculture or laboratory procedures. This term underscores the importance of human involvement in cultivation and research, particularly in developing effective agricultural techniques for biofortification.
26) Post:
In research, 'post' often denotes the stage following specific interventions. In the study, it may indicate post-harvest phases or assessments after breeding to evaluate improvements in rice's nutrient content. Understanding these stages is crucial for successful implementation of agricultural innovations.
Other Science Concepts:
Discover the significance of concepts within the article: ‘Mapping candidate genes for grain iron and zinc in rice.’. Further sources in the context of Science might help you critically compare this page with similair documents:
Micronutrient malnutrition, Significant association, Microbial analysis, Phenotypic Characterization, Randomized block design, Candidate gene approach, Genetic variability.