Optimizing parameters for improved tannase production from a novel bacterium.
Journal name: World Journal of Pharmaceutical Research
Original article title: Optimisation of parameters for enhanced tannase production from a novel bacterial producer
The WJPR includes peer-reviewed publications such as scientific research papers, reports, review articles, company news, thesis reports and case studies in areas of Biology, Pharmaceutical industries and Chemical technology while incorporating ancient fields of knowledge such combining Ayurveda with scientific data.
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Sarang Sheela, Vichare Smita and Vora Dipak
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World Journal of Pharmaceutical Research:
(An ISO 9001:2015 Certified International Journal)
Full text available for: Optimisation of parameters for enhanced tannase production from a novel bacterial producer
Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research
Doi: 10.20959/wjpr20166-6455
Copyright (license): WJPR: All rights reserved
Summary of article contents:
Introduction
Tannase (E.C. 3.1.1.20) is an important inducible enzyme that hydrolyzes hydrolysable tannins, releasing glucose and gallic acid, which are highly valuable in various industries such as food, beverage, chemical, and pharmaceuticals. The increasing demand for cost-effective gallic acid has necessitated the exploration of effective methods for enhancing tannase production. The research presented focuses on the isolation and optimization of tannase production from a novel bacterial source, emphasizing environmentally friendly practices and the potential commercial applicability of the findings.
Isolation of Tannase Producers
The study began with the collection of 20 soil samples from various locations around Mumbai and Thane, which were screened for tannase-producing bacteria. The screening involved enriching the soil samples in nutrient broth and subsequently isolating bacteria on minimal agar plates containing tannic acid as a growth substrate. Various assays, including swarm plate assays, visual detection tests, and paper chromatography, were employed to identify potential high-activity tannase producers. Ultimately, 15 isolates demonstrating significant tannase activity were confirmed, and one particular strain, identified as Serratia marcescens, was selected for further optimization studies.
Media Optimization for Tannase Production
The research explored optimization of media components to enhance tannase yields from Serratia marcescens. Initial experiments utilized a basal medium with specific concentrations of nutrients, followed by adjustments in key physical parameters like temperature, pH, aeration, and agitation. Optimal conditions were identified at an incubation temperature of 37°C and a pH of 6. Various nitrogen and carbon sources were utilized, revealing that ammonium chloride (0.1%) and sucrose (0.05%) were particularly effective in maximizing tannase production in conjunction with 1.6% tannic acid.
Physical Conditions Affecting Production
In addition to media composition, the study assessed the impact of physical conditions on the enzyme's production. The results indicated that Serratia marcescens thrived under aerobic static conditions, demonstrating the importance of an optimal balance of oxygen for growth while avoiding excessive aeration that could hinder olive activity. The overall findings highlighted a notable enhancement in tannase production, increasing by 1.4 times from initial activity levels, emphasizing the critical role of condition optimization in bioprocessing.
Conclusion
The research successfully demonstrated that Serratia marcescens could be a potent producer of tannase enzyme under optimized conditions. The final recorded tannase activity was 1.115 U/mL, significantly higher than previously reported activities for other bacterial species under similar circumstances. The strategies employed for optimization of media composition and cultivation conditions provide a promising blueprint for future efforts aimed at large-scale tannase production. These findings pave the way for potentially commercial applications of tannase and its derivatives, further underscoring the enzyme's industrial value.
FAQ section (important questions/answers):
What is tannase and what are its main applications?
Tannase is an inducible enzyme that hydrolyses tannins, producing glucose and gallic acid. Its applications span the food, beverage, chemical, and pharmaceutical industries, where it is utilized for producing gallic acid and as a clarifying agent in beverages.
How was the novel tannase producer isolated in this study?
Soil samples from Mumbai and Thane were enriched in nutrient broth, followed by isolation on minimal agar plates containing tannic acid. Individual colonies were selected and further screened for their tannase activity using specific assays.
Which bacterial strain was identified as the best tannase producer?
The selected bacterium with the highest tannase activity was identified as Serratia marcescens through biochemical tests and 16s rRNA analysis.
What conditions were found optimal for tannase production?
Optimal conditions for tannase production were at 37°C, pH 6, using 1.6% tannic acid and 0.05% sucrose in aerobic static conditions, resulting in a final activity of 1.115 U/ml.
How were media components optimised to enhance tannase yields?
Media optimisation involved varying nitrogen and carbon sources, including ammonium chloride and sucrose, as well as the concentration of tannic acid. This helped achieve a 1.4-fold increase in tannase production.
What significance does this research have for commercial exploitation?
This study highlights that Serratia marcescens has promising potential as a tannase producer, offering economically viable methods for production that could be scaled for commercial applications in various industries.
Glossary definitions and references:
Scientific and Ayurvedic Glossary list for “Optimizing parameters for improved tannase production from a novel bacterium.”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.
1) Activity:
In the context of this research paper, 'activity' refers to the tannase enzyme's capability to catalyze reactions that break down tannins into gallic acid and glucose. Measuring tannase activity is crucial for evaluating the efficiency of the bacterial isolate, 'Serratia marcescens', in producing this enzyme under optimal conditions, which has significant industrial applications.
2) Food:
The term 'food' is relevant as tannase has applications in the food industry, particularly in its role in the production of food preservatives. It helps in creating antioxidants and other compounds through the breakdown of tannins, thereby enhancing food safety and shelf life, while possibly also improving flavors.
3) Table:
In scientific literature, a 'table' serves as a concise format for presenting data, such as experimental results concerning tannase production. Tables provide visual clarity for comparing different conditions affecting enzyme activity or other parameters, allowing readers to quickly grasp experimental outcomes and methodologies employed in the study.
4) Soil:
The 'soil' from which bacterial isolates were obtained is essential for understanding the environmental context of this research. Soil is a natural reservoir for diverse microorganisms, including those capable of producing tannase. The collection of soil samples facilitates the discovery of novel tannase-producing bacteria, vital for industrial applications.
5) Medium:
In microbiological studies, 'medium' refers to the nutrient solutions used to cultivate bacteria. The medium composition can significantly influence the growth of the bacterial isolate and the production of tannase. Optimizing the medium with carbon and nitrogen sources is critical for enhancing enzyme yield, as highlighted in the study.
6) Maharashtra (Maharastra, Maha-rashtra):
The inclusion of 'Maharashtra' designates the geographic location where the research was conducted. This information contextualizes the study within India's diverse ecological systems and variations in soil microbiota. It may also reflect the availability of specific strains of bacteria known for tannase production, emphasizing regional biodiversity.
7) Species:
'Species' pertains to the specific bacterial isolate identified as 'Serratia marcescens' in this research. Understanding the species is crucial, as different bacterial species may exhibit varying levels of tannase production. Identifying the species helps in comprehending its potential for commercial utilization in industries reliant on tannase.
8) Sarang:
'Sarang' refers to one of the authors of the study and underscores the collaborative nature of scientific research. Author attribution is significant, as it acknowledges contributions and establishes credibility for the findings presented in the paper. This recognition is vital in academic and research communities for future references.
9) India:
'India' represents the country where the research originated. The relevance of this designation lies in presenting the study in a national context where agricultural and industrial strategies may benefit from local research on biotechnological applications, such as tannase production, which can enhance food processing and environmental sustainability.
10) Water:
'Water' is implicitly relevant in the sense that tannase-producing bacteria, including those selected in this study, might also be found in aquatic environments. Understanding the relationship between tannase production and water sources highlights potential applications for renewable resources and bioremediation processes in environments impacted by tannins.
11) Sabu:
The name 'Sabu' references one of the authors acknowledged for prior research in the paper. Including it establishes a continuity of knowledge and collaboration in the field of tannase research. Authorial presence links the current findings to previous studies, emphasizing credibility and the evolution of research insights.
12) Road:
'Road' is associated with the specific locality, 'L.N Road,' indicating the site of Ramnarain Ruia College in Mumbai. Such details provide context to the institutions involved in the research, which can be of interest for academic relationships, collaborations, and knowledge dissemination regarding biotechnological advancements in enzyme production.
13) Antibiotic (Antibacterial):
The term 'antibacterial' is crucial as it highlights one of the significant applications of gallic acid, produced by the action of tannase. The study's relevance extends to pharmaceuticals, as antibacterial compounds synthesized from gallic acid could lead to innovative treatments, making the research pertinent to public health considerations.
14) Purification:
'Purification' refers to the process of isolating the tannase enzyme from the bacterial extract to ensure it is free from contaminants. This step is necessary to evaluate the enzyme's properties accurately and its potential applicability in various industries. Effective purification techniques enhance the enzyme's commercial viability and performance.
15) Discussion:
'Discussion' represents a section in the research paper where results are analyzed and interpreted. This part is pivotal for contextualizing findings within the broader scientific landscape, examining implications, and suggesting future research directions. It facilitates critical thinking about the outcomes and how they align with or challenge existing knowledge.
16) Substance:
'Substance' generally refers to a particular matter, in this case, likely pertinent to the tannins, gallic acid, or other compounds discussed in the research. Understanding these substances is essential for elucidating biochemical pathways and their industrial importance, making the study of their interactions crucial in enzyme production.
17) Sharman (Sarma, Sharma, Sarman):
'Sharma' highlights one of the referenced authors associated with the methodology used for assessing tannase activity. Acknowledging established methods by veteran researchers adds validity to the current study's protocol and demonstrates a rigorous scientific approach, enhancing the credibility of the results obtained.
18) Animal:
'Animal' pertains to the potential impact of tannase applications in industries related to animal health products or feeds. The discussion of its capabilities in synthesis relating to animal products makes the research multi-dimensional, linking enzyme production not only to food and pharmaceuticals but also to veterinary applications.
19) Smita:
'Smita' denotes one of the authors contributing to the research. Author recognition is important in establishing the intellectual trustworthiness of the work presented. It acknowledges collaborative efforts in scientific inquiry and promotes accountability, allowing for the tracing of contributions in future citations and research advancement.
20) Thane:
'Thane' references the location of soil sample collection in the study. This information provides geographical context, indicating diverse microbial ecosystems from which bacterial isolates were sourced. Such locality aspects can contribute to understanding the ecological dynamics of tannase production and offer insights into biotechnological applications based on regional biodiversity.
21) Sugar:
'Sugar' pertains to the carbon sources utilized in the media for bacterial growth and tannase production. The influence of various sugars on enzyme yield is critical in optimizing conditions for maximal activity. This relevance aligns with the study's focus on the impact of nutritional components on industrial enzyme production.
22) Wine:
The term 'wine' is relevant due to tannase's role in the beverage industry, particularly in wine production. Tannase helps clarify and improve the taste of wines by degrading tannins that may cause astringency. This application showcases the enzyme's importance in enhancing sensory qualities and consumer satisfaction in beverages.
23) Drug:
'Drug' relates to the pharmaceutical applications of gallic acid derived from tannase activity. The significance lies in synthesizing antibacterial agents for medical use, thus highlighting the scope of tannase enzyme production in drug development. Understanding this relationship emphasizes the study's potential impact on healthcare and therapeutic advancements.
Other Science Concepts:
Discover the significance of concepts within the article: ‘Optimizing parameters for improved tannase production from a novel bacterium.’. Further sources in the context of Science might help you critically compare this page with similair documents:
Physical condition, Bacterial isolate, Serratia marcescens, Temperature and pH, Growth conditions, Media components, Tannery effluent, Environmentally friendly method, Nitrogen and carbon sources, Tannic acid concentration, Screening method.