Journal name: World Journal of Pharmaceutical Research
Original article title: Micropropagation of justicia adhatoda
The WJPR includes peer-reviewed publications such as scientific research papers, reports, review articles, company news, thesis reports and case studies in areas of Biology, Pharmaceutical industries and Chemical technology while incorporating ancient fields of knowledge such combining Ayurveda with scientific data.
This page presents a generated summary with additional references; See source (below) for actual content.
Subtitle: medicinal plant of family acanthaceae.
Original source:
This page is merely a summary which is automatically generated hence you should visit the source to read the original article which includes the author, publication date, notes and references.
Alka Sharma, Vijay R. Kumar and Ashwani Kumar
World Journal of Pharmaceutical Research:
(An ISO 9001:2015 Certified International Journal)
Full text available for: Micropropagation of justicia adhatoda
Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research
Doi: 10.20959/wjpr20167-6651
Copyright (license): WJPR: All rights reserved
Download the PDF file of the original publication
Summary of article contents:
Introduction
The increasing global use of traditional medicines has resulted in a rapid depletion of medicinal plants in their natural habitats. This depletion is compounded by difficulties in successful cultivation and concerns regarding genetic fidelity. Traditional propagation methods have led to reduced secondary metabolite content in cultivated plants compared to their wild counterparts. In this context, plant tissue culture has emerged as a promising technique for the mass propagation of medicinal plants. The study focuses on Justicia adhatoda, a significant medicinal plant, exploring its micropropagation and metabolic engineering for sustainable use.
Micropropagation Techniques in Justicia adhatoda
The investigation aimed to develop an efficient in vitro protocol for the high-frequency regeneration of Justicia adhatoda using various explants, modified basal media, and plant growth regulators (PGRs). Explants were obtained from healthy plants, sterilized, and cultured on Murashige and Skoog (MS) medium supplemented with different concentrations of auxins and cytokinins. The best callus induction rate of 80% was observed when nodal segments were cultured on the optimized media. This indicates that the choice of explant and PGR composition is crucial for enhancing the regenerative capacity of the plant in vitro.
Role of Growth Regulators in Callus Induction
The study further explored the effect of various growth regulators on callus formation from the nodal segments of Justicia adhatoda. It was found that the combination of Naphthalene Acetic Acid (NAA), Benzyladenine (BA), Kinetin (Kn), and 2,4-Dichlorophenoxyacetic Acid (2,4-D) yielded the highest callus induction rate (81.0%). This highlights the importance of optimizing growth regulator concentrations in plant tissue culture, as it significantly influences the success of callus formation, which is essential for further propagation and secondary metabolite production.
Secondary Metabolite Production Potential
In addition to callus induction, the study evaluated the effects of different growth regulators on shoot formation. The optimal combination for shoot induction was found to be IAA, BA, and Kn at specific concentrations. The ability to produce shoots successfully is vital for establishing a protocol that can facilitate the production of valuable secondary metabolites, like vasicine, which is known for its medicinal properties. The findings emphasize the potential of Justicia adhatoda tissue culture systems for the extraction and synthesis of bioactive compounds used in traditional medicine.
Conclusion
The research on Justicia adhatoda demonstrates that plant tissue culture techniques can be successfully applied to enhance the propagation of medicinal plants and secure their continuous availability for traditional and modern medicinal applications. By understanding the role of various growth regulators and optimizing the micropropagation process, it is possible to support the conservation of this species and ensure sustainable over-exploitation. The study paves the way for future investigations aimed at maximizing the production of bioactive metabolites, reinforcing the significance of Justicia adhatoda in herbal medicine and its contributions to healthcare.
FAQ section (important questions/answers):
What is the significance of Justicia adhatoda in traditional medicine?
Justicia adhatoda, also known as Adhatoda vasica, is significant as it contains alkaloids like vasicine, used in Ayurvedic medicine for treating respiratory diseases, bronchitis, tuberculosis, and various allergies.
What are the challenges in cultivating medicinal plants like Justicia adhatoda?
Challenges include rapid depletion of natural populations, low seed germination, and ineffective conventional propagation methods, leading to concerns over genetic fidelity and reduced secondary metabolite production.
How does plant tissue culture aid in conserving medicinal plants?
Plant tissue culture provides an efficient method for the clonal propagation of selected plants, preserving genetic fidelity, and facilitating the large-scale production of secondary metabolites without depleting natural resources.
What role do different growth regulators play in callus induction?
Different growth regulators in the medium influence callus formation. The best results for Justicia adhatoda were achieved with specific combinations of NAA, BA, and 2,4-D, achieving high percentage callus induction.
What are the key findings regarding the callus induction for Justicia adhatoda?
The study found that the best callus induction (80.0%) occurred using nodal segments on Murashige and Skoog medium, with specific growth regulator concentrations enhancing the induction process.
Why are secondary metabolites important in medicinal plants?
Secondary metabolites play a crucial role in the medicinal properties of plants, providing therapeutic benefits and enhancing the plant's ability to resist environmental stresses, thus holding significant pharmaceutical value.
Glossary definitions and references:
Scientific and Ayurvedic Glossary list for “Micropropagation of justicia adhatoda”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.
1) Sharman (Sarma, Sharma, Sarman):
Sharma refers to one of the authors of the research paper, indicating their contribution to the study of micropropagation techniques for medicinal plants, particularly Justicia adhatoda. The author's presence highlights collaboration and expertise in the field of botany and plant tissue culture, crucial for advancing agricultural and medicinal knowledge.
2) Medium:
In this context, 'medium' refers to the nutrient solution used for plant tissue culture. It provides essential nutrients, hormones, and conditions necessary for the growth and development of plant cells and tissues in vitro. The choice of medium significantly affects the success of micropropagation and biosynthetic processes.
3) Kumar:
Kumar is another author involved in the research study, contributing to the expertise in plant propagation and metabolic studies. The inclusion of Kumar underscores the collaborative efforts of multiple researchers to explore techniques for the sustainable cultivation of medicinal plants, essential for both conservation and pharmaceutical applications.
4) Rajasthan:
Rajasthan, the largest state in India, is significant for its rich diversity in medicinal plants, including Justicia adhatoda. Its unique climate and ecological conditions allow for the study of plant propagation techniques tailored to arid regions, addressing issues like overexploitation and the need for sustainable practices in herb cultivation.
5) Medicine:
Medicine in this context refers to the use of plants, such as Justicia adhatoda, in traditional and modern healthcare. The focus is on exploring the pharmacological properties and therapeutic potentials of plant-derived compounds, which is crucial for drug development and the conservation of valuable plant species used in traditional remedies.
6) Table:
The term 'Table' refers to the presentation of data summarizing the results of the experiments, such as callus induction rates and effects of various growth regulators. Tables organize information clearly and concisely, allowing researchers to easily interpret and compare results in relation to their hypotheses.
7) Species:
Species refers to specific groups of organisms, in this case, focusing on the plant species Justicia adhatoda. Understanding plant species' traits and behaviors is crucial for conservation efforts, studying ecological interactions, and exploring medicinal uses within ethnobotany, as well as for sustainable agricultural practices.
8) Adhatoda vasica:
Adhatoda vasica, known for its medicinal properties, particularly in Ayurvedic medicine, is a focus of this research. Its significance lies in its use for treating respiratory conditions. The study aims to optimize propagation and metabolite production to ensure the sustainable supply of this important medicinal species.
9) Shooting:
Shooting in plant tissue culture refers to the process of shoot formation from cultured explants. The generation of shoots is a critical phase in micropropagation, facilitating the eventual production of whole plants. Optimizing the conditions for shooting can significantly enhance the propagation success of medicinal plants.
10) Jaipur:
Jaipur, the capital of Rajasthan, is where the study is conducted, indicating the geographical relevance of the research. The presence of a research institution in this city supports local efforts to explore and document the medicinal plant diversity in the region, contributing to both scientific and cultural knowledge.
11) Botany:
Botany, the scientific study of plants, is foundational to this research. Knowledge of plant biology, physiology, and ecology is essential for developing effective propagation techniques and understanding the medicinal and ecological roles of species like Justicia adhatoda in their natural habitats.
12) Vasaka (Vashaka):
Vasaka is the common name for Justicia adhatoda, emphasizing its role in traditional medicine. Recognizing local names enhances cultural understanding by linking scientific research with traditional knowledge systems, which can aid in conservation and sustainable usage of local biodiversity for medicinal purposes.
13) India:
India, noted for its rich flora and diverse traditional medicinal practices, serves as the geographical context for the research. The study's relevance in India lies in addressing issues of plant preservation, sustainable use of natural resources, and integration of traditional knowledge with modern scientific methods.
14) Water:
Water is crucial for the successful establishment and maintenance of plant tissue cultures. Proper hydration ensures that plant cells remain viable and can effectively absorb nutrients from the culture medium, which is critical for growth, cell division, and eventual organogenesis in cultured explants.
15) Tuberculosis:
Tuberculosis (TB) is a serious infectious disease that the extract of Justicia adhatoda has been reported to help treat. Focusing on its potential role against TB underscores the importance of researching medicinal plants in finding alternative therapies for combating multidrug-resistant strains of this disease.
16) Accumulation (Accumulating, Accumulate):
Accumulation refers to the buildup of valuable secondary metabolites in plant tissues, which are often tied to their therapeutic properties. Understanding how to enhance the accumulation of such compounds through tissue culture techniques can lead to improving the commercial viability of medicinal plants.
17) Habitat:
Habitat denotes the natural environment where Justicia adhatoda grows. Protecting the habitat is crucial for conservation efforts. Understanding the interplay between habitat preservation and the sustainable use of plants for medicinal purposes is essential for maintaining biodiversity and ecological balance.
18) Surface:
Surface refers to the exterior of the plant tissues, particularly concerning sterilization processes before culturing. It is important to ensure that explants are free from contaminants that could compromise the culture's success, impacting the study of plant propagation and secondary metabolite production.
19) Drug:
Drug refers to medicinal compounds derived from plants like Justicia adhatoda. The research aims to explore the optimization of plant cultures for enhanced production of bioactive compounds, which can lead to the development of new pharmaceutical formulations for various health conditions.
20) Tar:
Thar, or the Thar Desert in India, is noted for its rich diversity of medicinal plants including Justicia adhatoda. The study may leverage this regional significance to address conservation and sustainable use while highlighting local knowledge and practices related to medicinal plant utilization.
21) Pharmacological:
Pharmacological pertains to the effects of drugs and their mechanisms of action. The relevance of studying plant-based compounds includes understanding their pharmacological properties to develop new treatments, as traditional medicine often relies on the efficacy of plants in combating various ailments.
22) Transformation (Transform, Transforming):
Transformation in this context may refer to genetic transformation techniques used to enhance plants' characteristics, including stress tolerance and metabolite production. This approach is crucial in biotechnology, targeting the improvement of plant varieties for better pharmaceutical applications.
23) Inflammation:
Inflammation indicates a biological response often treated using medicinal plants. Researching the effects of compounds derived from Justicia adhatoda on inflammation can contribute valuable information for developing anti-inflammatory therapies and underscore the plant's traditional uses in medicine.
24) Discussion:
Discussion is a crucial section in scientific research papers where authors interpret and analyze their findings. This section provides insights into the implications of the results, compares them with existing literature, and suggests future directions for research in tissue culture and medicinal applications.
25) Science (Scientific):
Scientific underscores the nature of the research which is based on empirical methods and evidence. The application of scientific principles to the study of medicinal plants, including Justicia adhatoda, enhances understanding and facilitates the integration of traditional knowledge with modern medicinal research.
26) Flavonoid:
Flavonoids are a class of secondary metabolites found in many plants, including Justicia adhatoda, with known antioxidant and anti-inflammatory properties. Understanding their biosynthesis and functions enhances the significance of these compounds in therapeutics and the overall health benefits associated with medicinal plants.
27) Cutting:
Cutting refers to a method of plant propagation where sections of a plant are used to grow new plants. This conventional method may often be compared to in vitro techniques; optimizing both methods is essential for successful cultivation and conservation of medicinal species.
28) Allergy:
Allergy indicates an adverse immune response that can be treated with certain medicinal plants. Research into the effects of Justicia adhatoda on allergic reactions could enhance the understanding of its medicinal value and promote its use in traditional and modern therapeutic practices.
29) Maurya:
Maurya is an author associated with the research, contributing knowledge and expertise in the field of plant biology. The collaboration of various authors like Maurya illustrates the multi-disciplinary effort needed to explore complex topics like plant propagation and pharmaceutical applications.
30) Suthar:
Suthar is another author involved in this study, highlighting the importance of collaborative research in advancing knowledge about medicinal plants. Their contributions and findings form the basis for deeper investigation into tissue culture methods and secondary metabolite production.
31) Family:
Family, in botanical taxonomy, classifies plants with shared characteristics. Justicia adhatoda belongs to the Acanthaceae family, which is essential for understanding the phylogenetic relationships among plants, their common traits, and how this classification can influence medicinal usage and botanical studies.
32) Nature:
Nature refers to the natural environment from which medicinal plants like Justicia adhatoda are sourced. Understanding the ecological dynamics and conservation needs of these plants is vital for sustainable harvesting practices and preserving biodiversity amid increasing demands for traditional herbs.
33) Saxena (Sakshena, Saksena):
Saxena is also an author contributing to the study, reflecting the collective input from multiple researchers. Their collaborative work emphasizes the need to incorporate diverse perspectives in understanding complex plant systems, especially regarding propagation and the medicinal properties of flora.
34) Study (Studying):
Study refers to the structured investigation of plant propagation techniques and the exploration of medicinal properties in Justicia adhatoda. The results from such studies contribute to the broader understanding of plant biotechnology and sustainable practices in the conservation of medicinal plants.
35) Tree:
Tree generally refers to a large perennial plant. In the context of this research, although Justicia adhatoda is a shrub, it illustrates the importance of various plant structures in understanding propagation methods, ecological roles, and their applications in traditional medicine.
36) Crop:
Crop signifies cultivated plants, crucial for sustenance and trade. Understanding how to micropropagate medicinal crops like Justicia adhatoda addresses agricultural practices and helps fulfill the increasing demand for herbal medicines in both local and global markets.
37) Rich (Rch):
Rich denotes the abundance of medicinal plant species in a given area, like in Rajasthan, which emphasizes the ecological importance of preserving such resources. A rich diversity contributes to traditional medicine, food security, and sustainable practices in agriculture and conservation.
38) Viru:
Viru is not explicitly identified in the provided context but may hint at a specific plant or a term related to the medicinal use or ecological context within the region. It signifies an aspect of diversity that may need further exploration regarding its properties or uses.
Other Science Concepts:
Discover the significance of concepts within the article: ‘Micropropagation of justicia adhatoda’. Further sources in the context of Science might help you critically compare this page with similair documents:
Hiv, Medicinal plant, Traditional medicine, Secondary metabolite, Multidrug resistant tuberculosis, Vasicine, Plant tissue culture, Callus induction, Murashige and Skoog medium, Indole Acetic Acid, Over exploitation, Plant growth regulator.