Phylogenetic analysis of two species of sea horse genus
hippocampus using cytochrome b of mitochondrial dna
Journal name: World Journal of Pharmaceutical Research
Original article title: Phylogenetic analysis of two species of sea horse genus
The WJPR includes peer-reviewed publications such as scientific research papers, reports, review articles, company news, thesis reports and case studies in areas of Biology, Pharmaceutical industries and Chemical technology while incorporating ancient fields of knowledge such combining Ayurveda with scientific data.
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Subtitle: hippocampus using cytochrome b of mitochondrial dna
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Dr. Virupakshaiah D.B.M.
World Journal of Pharmaceutical Research:
(An ISO 9001:2015 Certified International Journal)
Full text available for: Phylogenetic analysis of two species of sea horse genus
Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research
Doi: 10.20959/wjpr20168-6743
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Summary of article contents:
Introduction
Seahorses, belonging to the Syngnathidae family, exhibit unique morphological traits distinguishing them from other fish, such as a prehensile tail and a distinctive head position. This study focuses on the phylogenetic relationships of two species of seahorses from the genus Hippocampus, specifically Hippocampus kuda. Using mitochondrial DNA's cytochrome b gene, this research investigates genetic connectivity and evolutionary patterns among seahorse populations in Indian coastal waters, leveraging the importance of molecular markers in understanding marine biodiversity.
Phylogenetic Relationships
One important aspect of this study is the phylogenetic analysis conducted using the cytochrome b gene sequences. The results indicated that Hippocampus kuda was closely associated with haplotype IND 338.5, while its populations showed signs of a mixed distribution with haplotypes TH16 and JAP4. This suggests that the Indian populations of Hippocampus kuda are not genetically isolated but are instead interconnected with other populations in different geographical regions. The analysis reveals nuances in genetic divergence, underscoring the evolutionary relationships within and between these seahorse populations.
Sample Collection and DNA Analysis
Sample collection involved obtaining seahorse tails from fishers in the Gulf of Mannar along the southeast coast of India, which were then preserved in ethanol for DNA extraction. Standard protocols were employed for the isolation of DNA from these samples, ensuring high-quality DNA suitable for subsequent analysis. The process included quantifying the extracted DNA using UV spectrophotometry and assessing its integrity via agarose gel electrophoresis. This careful methodology is crucial for obtaining reliable genetic data necessary for effective phylogenetic analysis.
Molecular Amplification and Sequencing
To amplify the target mitochondrial DNA, primers specific to the cytochrome b gene were designed and utilized in Polymerase Chain Reaction (PCR). The successful amplification of the DNA was followed by purification and sequencing processes. By employing advanced sequencing techniques, including BigDye terminator chemistry, the resulting sequences were analyzed to construct a comprehensive genetic profile. This detailed sequencing provided a foundation for further genetic distance calculations and phylogenetic relationships among and between the studied seahorse species.
Conclusion
This study investigated the genetic relationships and connectivity of the genus Hippocampus, particularly focusing on Hippocampus kuda within Indian coastal waters, using mitochondrial DNA for the analysis. The findings highlighted that Hippocampus kuda populations are not uniquely distributed, emphasizing the complexity of their genetic structure and interconnections to other haplotypes. This work contributes to the broader understanding of seahorse genetics and conservation, revealing insights into evolutionary patterns critical for the management and preservation of seahorse species in varying marine environments.
FAQ section (important questions/answers):
What is the focus of the research on Hippocampus species?
The research focuses on the phylogenetic analysis of two seahorse species, specifically Hippocampus kuda and Hippocampus trimaculatus, using cytochrome b mitochondrial DNA to explore their genetic connectivity.
What method was used for DNA isolation in the study?
DNA isolation was carried out using the standard EDTA method, involving chopping tail samples and quantifying the DNA using a spectrophotometer and agarose gel electrophoresis.
How was the DNA amplification performed in this research?
DNA amplification was performed using PCR with specific primers designed based on optimal GC content and annealing temperature adjustments to ensure successful amplification of the cytochrome b gene.
What conclusions were drawn regarding Hippocampus kuda populations?
The analysis revealed that Hippocampus kuda populations are not uniquely distributed in nature, indicating mixed genetic populations with specific haplotypes observed across different regions.
What were the key findings from the phylogenetic analysis?
Phylogenetic analysis showed that Hippocampus kuda is closely related to haplotype IND 338.5, with evidence of mixed populations involving TH16 and JAP4 haplotypes within Indian waters.
Why are seahorses significant in this study of evolution?
Seahorses are significant due to their unique biological traits, ecological diversity, and the evolutionary insights they provide regarding marine species distribution and connectivity across regions.
Glossary definitions and references:
Scientific and Ayurvedic Glossary list for “Phylogenetic analysis of two species of sea horse genus”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.
1) Species:
In biological classification, 'species' refers to a group of organisms capable of interbreeding and producing fertile offspring. The study specifically analyzes two species of seahorse within the genus Hippocampus. Understanding species diversity is essential for conservation efforts and studying evolutionary relationships within marine ecosystems.
2) Kuda:
'Kuda' denotes the specific species Hippocampus kuda, which is analyzed in this research. This seahorse species inhabits coastal waters and shows varying genetic traits. Its significance lies in understanding the distribution, genetic variation, and ecological adaptation of seahorses in Indian waters and potentially highlighting conservation needs.
3) Water:
Water is the primary habitat for seahorses, including Hippocampus kuda. The study emphasizes the importance of coastal water environments in supporting biodiversity. Researching the genetic connectivity between populations in these habitats aids in understanding how marine organisms adapt to their aquatic ecosystems and the effects of environmental changes.
4) Horse:
The term 'horse' in this context relates to seahorses, which, despite their name, are unique members of the Syngnathidae family. They exhibit different physical and biological characteristics distinct from true horses. Understanding seahorses' unique adaptations helps clarify their evolutionary history and ecological importance in marine biodiversity.
5) Indian:
'Indian' references the geographic and ecological context of the study, which focuses on the coastal waters of India. The Indian Ocean's varied marine life and ecological dynamics make it crucial for understanding species distribution and genetic connectivity among seahorses, particularly in relation to global biodiversity.
6) Study (Studying):
The term 'study' signifies the systematic investigation undertaken to analyze the phylogenetic relationships and genetic variation among seahorse species. It reflects scientific inquiry aimed at uncovering aspects of marine biology and has implications for biodiversity conservation and management strategies in marine environments.
7) Genu:
'Genus' refers to a rank in the biological classification system used to group species sharing common characteristics. In this research, Hippocampus represents the genus name for seahorses. Understanding phylogenetic relationships at the genus level is essential for elucidating evolutionary patterns and species divergence within marine taxa.
8) India:
'India' provides the geographical and ecological framework for the research, indicating that the study's focus is on the Indian coastal ecosystem. The marine biodiversity unique to Indian waters necessitates specialized conservation efforts and ecological studies to understand the adaptations and connectivity of resident marine species like seahorses.
9) Tree:
In this context, 'tree' refers to the phylogenetic tree, a diagrammatic representation illustrating evolutionary relationships among various species. The phylogenetic tree derived from the study aids in visualizing genetic connections and divergence among Hippocampus species, helping researchers deduce patterns of evolution and speciation.
10) Line:
'Line' may refer to the Wallace Line, a biogeographical boundary that separates species distributions. This concept is essential in understanding evolutionary processes and geographic barriers affecting marine populations, including seahorses in the Indian Ocean, highlighting how geography influences genetic diversity and species similarity.
11) Karnataka:
Karnataka is the state in India where the research was conducted, highlighting the local ecological significance and diversity of marine life in this region. By studying seahorses within Karnataka's coastal waters, this research aims to enhance understanding and conservation efforts of unique marine species facing environmental threats.
12) Habitat:
'Habitat' refers to the natural environment where an organism lives. This study examines specific habitats of Hippocampus kuda, differentiating between types (e.g., shallow waters, seagrass, mangroves). Understanding habitats is crucial for conservation efforts, as it influences seahorse distribution, behavior, and ecological interactions.
13) Nature:
In this context, 'nature' pertains to the intrinsic characteristics and behaviors of organisms within marine ecosystems. The study examines how seahorse species interact with their environment and each other naturally while adapting to ecological variables and genetic speciation pressures, which are vital for preserving biodiversity.
14) Purification:
'Purification' refers to the processes used to isolate DNA from the seahorse samples for further genetic analysis. Ensuring high-quality DNA is essential for accurate sequencing outcomes and interpretations of genetic information, which can influence conservation strategies and ecological understandings in marine biology.
15) Discussion:
The term 'discussion' represents a critical part of the research paper where findings are interpreted, implications are explored, and comparisons with existing literature are made. This section is fundamental for contextualizing the results within broader scientific discourse, contributing to knowledge on seahorse diversity and conservation.
16) Reflecting:
'Reflecting' is used to convey the idea of examining or representing the genetic and evolutionary patterns seen in the studied seahorse populations. The relationship between population distributions and environmental factors is revealed, guiding further research and conservation efforts, as well as enhancing understanding of marine biodiversity.
17) Quality:
'Quality' relates to the integrity of the DNA samples obtained during the study. High-quality DNA is essential for reliable molecular analysis, influencing the outcomes of genetic studies and phylogenetic relationships. Ensuring quality through precise isolation and quantification methods is crucial for meaningful scientific results.
18) Surface:
The term 'surface' refers to external contaminants present on the seahorse samples prior to DNA extraction. Cleaning the samples is vital to ensure that any environmental DNA does not interfere with the analysis, thereby maintaining the integrity of the research focused on the species' genetic makeup.
19) Family:
'Family' is a taxonomic rank in biological classification and in this context refers to Syngnathidae, the family that includes seahorses. Understanding familial relationships helps clarify evolutionary patterns and biological similarities among diverse marine organisms, aiding in the conservation of species and their habitats.
20) Reason:
'Reason' indicates the rationale behind studying Hippocampus species and analyzing their genetic diversity and phylogenetic relationships. The research aims to understand evolutionary processes, patterns of dispersal, and implications for conservation, addressing the need for knowledge about marine biodiversity in changing ecosystems.
21) Trader:
'Trader' signifies individuals involved in the collection and distribution of seahorses, highlighting human interactions with marine species. Traders play a role in the seahorse supply chain, which can impact conservation strategies, necessitating greater awareness of sustainable practices and species protection efforts in marine environments.
Other Science Concepts:
Discover the significance of concepts within the article: ‘Phylogenetic analysis of two species of sea horse genus’. Further sources in the context of Science might help you critically compare this page with similair documents:
Sample collection, Agarose gel electrophoresis, PCR amplification, Phylogenetic analysis, Mitochondrial DNA, Nucleotide substitution.