Characterization of Colletotrichum species in Kashmir apple orchards

| Posted in: Health Sciences Science

Journal name: World Journal of Pharmaceutical Research
Original article title: Characterization of colletotrichum species causing bitter rot of apples in kashmir orchards
The WJPR includes peer-reviewed publications such as scientific research papers, reports, review articles, company news, thesis reports and case studies in areas of Biology, Pharmaceutical industries and Chemical technology while incorporating ancient fields of knowledge such combining Ayurveda with scientific data.
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Author:

Gajendra Singh, Pushkar Choudhary, Rayees Ahmad, Rajveer Singh Rawat, Dr. Bhanwar Lal Jat


World Journal of Pharmaceutical Research:

(An ISO 9001:2015 Certified International Journal)

Full text available for: Characterization of colletotrichum species causing bitter rot of apples in kashmir orchards

Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research

Doi: 10.20959/wjpr201611-7220

Copyright (license): WJPR: All rights reserved


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Summary of article contents:

Introduction

The study detailed in this document focuses on the characterization of Colletotrichum species responsible for bitter rot in apples within the Kashmir region. The research highlights the significance of accurate pathogen identification for effective disease management in apple orchards, where various species of Colletotrichum have been noted to cause severe economic losses. Employing both morphology-based identification and molecular techniques including multi-locus gene sequencing and RAPD analyses, the authors aimed to establish the prevalence of specific Colletotrichum species and their pathogenic behaviors, as well as their sensitivity to commonly used fungicides.

Species Identification and Their Characteristics

The research identified five different species of Colletotrichum associated with bitter rot: C. fioriniae, C. nymphaeae, C. siamense, C. theobromicola, and C. fructicola. Notably, morphological analysis revealed distinct groups based on spore shape and colony characteristics, allowing the researchers to categorize the isolates accordingly. For instance, C. fioriniae was distinguished by its red pigmentation in culture, making it highly prevalent among the isolates. This study underscores the importance of using both morphological and molecular identification techniques to accurately diagnose species involved in apple rot, as similar morphological traits among species can complicate identification.

Pathogenicity and Aggressiveness of Species

Pathogenicity tests demonstrated significant differences in the disease-causing potential among different Colletotrichum species. Specifically, isolates from the C. gloeosporioides species complex displayed more aggressive characteristics, producing larger and deeper lesions compared to those from the C. acutatum species complex. Among these, C. siamense was found to be the most pathogenic, while C. fioriniae showed considerable aggressiveness as well. This information is crucial for growers in Kashmir as it can inform management practices that specifically target the most virulent forms of the pathogen, helping to reduce crop losses.

Fungicide Sensitivity and Management

The sensitivity of different Colletotrichum species to commonly used fungicides, including thiophanate-methyl, myclobutanil, trifloxystrobin, and captan, was also assessed. Results indicated a notable difference in tolerance levels between the two species complexes, with C. acutatum generally exhibiting a higher tolerance to tested fungicides compared to C. gloeosporioides. Moreover, within species of the gloeosporioides complex, variability in sensitivity was observed. This finding suggests that management strategies in the region could benefit from tailored fungicide applications based on the specific pathogen present, thereby potentially enhancing disease control measures and preventing economic loss.

Conclusion

In conclusion, the study highlights the complex interactions between various Colletotrichum species and their implications for apple production in Kashmir. Accurate identification and understanding of the pathogenicity and fungicide sensitivity of these species are essential for developing effective disease management strategies. The findings advocate for the importance of implementing integrated pest management practices that include cultural controls, sanitation, and precise fungicide application, catering to the specific characteristics of the pathogens present. Ongoing research into genetic diversity and field studies on pathogenicity will further contribute to our understanding of these problematic fungi and improve the efficacy of disease management in apple orchards.

FAQ section (important questions/answers):

What are the species causing bitter rot in Kashmir apples?

The study identified five species causing bitter rot: C. fioriniae, C. nymphaeae, C. siamense, C. theobromicola, and C. fructicola.

How were the Colletotrichum species identified in the study?

Identification was performed using morphological traits and molecular methods, including multi-locus gene sequencing of TUB2 and GAPDH genes.

What does the study reveal about fungicide sensitivity among species?

The study found that C. acutatum species complex isolates were more tolerant to fungicides than those from the C. gloeosporioides complex.

What was the most aggressive species identified in the study?

Colletotrichum siamense was found to be the most aggressive species, causing larger and deeper lesions than other species.

What pathogenicity differences exist among the identified Colletotrichum species?

Differences in pathogenicity were observed, with C. fioriniae causing larger lesions than C. nymphaeae, and C. siamense being the most aggressive overall.

Why is accurate identification of Colletotrichum species important for management?

Accurate pathogen identification is crucial for implementing effective disease management strategies, considering the differences in pathogenicity and fungicide sensitivities among species.

Glossary definitions and references:

Scientific and Ayurvedic Glossary list for “Characterization of Colletotrichum species in Kashmir apple orchards”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.

1) Species:
In the context of the study, 'species' refers to the different types of Colletotrichum fungi identified as causing bitter rot in apple trees. Understanding the distinct species is crucial for disease management, as each may exhibit different pathogenic characteristics, fungicide sensitivities, and ecological behaviors that influence crop health and yield.

2) Bitter:
'Bitter' describes the rot disease affecting apples, characterized by the lesions and decay it causes. The term is essential for identifying the type of fruit rot that significantly impacts apple production in Kashmir, highlighting the disease's economic and agricultural importance, and necessitating effective management strategies.

3) Kashmir:
Kashmir refers to the region in India where this study was conducted, significant for its apple orchards and agricultural practices. Its climate and geography influence the prevalence and characteristics of plant diseases, including bitter rot, thereby informing local farming practices and disease management solutions.

4) Disease:
'Disease' in this context relates to the bitter rot affecting apples, caused by specific Colletotrichum species. Understanding the nature of the disease is crucial for developing effective prevention and control measures, as it directly impacts agricultural productivity, fruit quality, and economic outcomes for farmers.

5) Study (Studying):
'Studying' refers to the systematic investigation of Colletotrichum species in apple orchards. This process of studying is essential for gaining insights into pathogen behaviors, understanding disease mechanisms, and developing effective strategies for managing bitter rot.

6) India:
India is the country where Kashmir is located, and it plays a significant role in the horticultural sector, particularly in apple production. Understanding the agricultural practices and challenges faced in India helps contextualize the study and its implications for fruit production and food supply logistics.

7) Relative:
'Relative' denotes comparisons made during the study, such as in terms of pathogenicity and fungicide sensitivity among different Colletotrichum species. This comparative approach is essential for identifying which species pose a greater threat to apple crops and for tailoring effective treatment strategies.

8) Tree:
'Tree' refers to the apple trees in Kashmir orchards that are subject to bitter rot. This term underlines the importance of healthy trees for fruit production and emphasizes the relevance of studying pathogens that affect their health and the overall yield of apples.

9) Gold (Golden):
'Gold' possibly refers to the Golden Delicious apple or as a descriptor for high quality, indicating economic value in produce. Gold-associated terms might highlight market desirability and branding strategies vital for fruit commercialization.

10) Water:
'Water' is a critical environmental factor influencing the development of plant diseases, including bitter rot. Its role in pathogen spread and fungal spore germination emphasizes the need for appropriate irrigation and management practices to mitigate disease outbreaks in orchards.

11) Crop:
'Crop' in this context signifies the apples being produced in Kashmir orchards. Apples are an essential agricultural output, and understanding the factors leading to crop losses, such as diseases, informs strategies to optimize production and maintain economic viability for farmers.

12) Valley:
'Valley' refers to the Kashmir Valley, a region known for its fertile land and rich horticulture. The valley's particular environmental conditions contribute to both the advantages and challenges of apple production, including susceptibility to diseases like bitter rot.

13) Field:
'Field' denotes the agricultural land where apple trees are cultivated. Understanding field conditions, pest interactions, and disease prevalence is crucial for effective farm management practices, facilitating targeted interventions to protect crops and maintain healthy yields.

14) Genu:
'Genu' likely refers to a genus within biological classification, stressing the scientific aspects of taxonomic categorization. Accurate identification of fungal genera, particularly Colletotrichum, is essential for effective disease diagnosis and implementing appropriate management practices in agriculture.

15) Rajasthan:
Rajasthan, where Bhagwant University Ajmer is located, contributes to the broader agricultural research ecosystem in India. Understanding regional differences in horticulture and diseases facilitates better management practices across diverse agricultural areas, including Kashmir's unique growing conditions.

16) Insect:
'Insect' points to the various pests that threaten apple crops in Kashmir. Identifying these pests and understanding their impact on fruit health underscores the importance of integrated pest management strategies to mitigate losses and enhance production sustainability.

17) Ajmer:
Ajmer is the city in Rajasthan housing Bhagwant University, where part of the research team is based. This location emphasizes the academic collaboration that supports agricultural research, using scientific inquiry to address local and regional agricultural challenges, including those faced in Kashmir.

18) Moth:
'Moth' refers to specific insect pests, like the codling moth, that infest apple fruits and can significantly damage crops. Understanding the impact of these pests on apple production emphasizes the need for effective pest management practices alongside disease control.

19) Knowledge:
'Knowledge' encompasses the insights gained from studying the Colletotrichum species and their effects on apple crops. Increased knowledge about disease dynamics is fundamental for developing effective management strategies that enhance agricultural productivity and ensure food security.

20) Surface:
'Surface' refers to the exterior part of apple fruit where infections may occur. The relevance of surface in disease management highlights how physical interactions between the fungi and the fruit skin can determine the severity of infections and subsequent yield losses.

21) Cola:
'Cola' may refer to the coca or cola family in relation to tropical agriculture, although its specific relevance in this context may require further clarification—likely as a point of relation to biochemical content or taste preferences among various fruit species.

22) Measurement:
Measurement relates to the quantifying of variables such as lesion size or growth rates of fungi in the study. Accurate measurement is crucial for validating research findings, comparing pathogenicity, and making informed decisions about disease management strategies.

23) Agriculture:
'Agriculture' refers to the science and practice of cultivating soil, growing crops, and raising animals. The broader agricultural context highlights the importance of research in improving productivity, managing diseases, and ensuring sustainable practices to secure food supply and farmers' livelihoods.

24) Similarity:
'Similarity' denotes the morphological and biological characteristics shared among different Colletotrichum species, which can complicate accurate identification. Recognizing similarities is essential for understanding disease dynamics and implementing appropriate management strategies based on species-specific traits.

25) Epidemic:
'Epidemic' typically refers to the rapid increase in disease incidence within a population. In the context of agriculture, understanding how environmental conditions can lead to epidemic outbreaks of bitter rot aids in developing proactive management strategies for crops.

26) Wounded:
'Wounded' refers to apple fruits that have been damaged, making them more susceptible to infections by pathogens like Colletotrichum. Wounded fruits serve as a focal point for disease spread, highlighting the importance of careful handling and effective pest management.

27) Medium:
'Medium' signifies the growth substrate used for culturing fungi in laboratory settings. The type of medium influences the growth characteristics of Colletotrichum species, impacting experimental outcomes and the understanding of their biology and pathogenicity.

28) Insect pest:
'Insect pest' relates to harmful insects that damage apple crops, exacerbating economic losses. Understanding the complex interactions between insect pests and plant diseases is vital for developing integrated pest management strategies to protect apple yield.

29) Observation:
'Observation' is the act of systematically monitoring and recording data during experiments. Careful observation is key to accurately understanding the dynamics of disease progression, pathogen behavior, and the efficacy of control measures in apple orchards.

30) Developing:
'Developing' pertains to the process of creating or improving methods for managing apple rot diseases. Focused development efforts are important for establishing effective practices that can enhance apple yields while minimizing economic losses for farmers.

31) Science (Scientific):
'Science' emphasizes the empirical methods and inquiry-based approaches employed in the study of Colletotrichum and bitter rot. The scientific method underpins research efforts aimed at advancing agricultural practices and improving disease management strategies.

32) Harvesting (Harvest):
'Harvest' relates to the period when apples are gathered from the orchard, directly linked to production success. Timing and methodology during harvest are crucial factors that impact the quality and marketability of the fruit.

33) Container:
'Container' refers to the vessels used for storing or transporting apple fruit during and after harvest. The choice of containers affects fruit quality, helps minimize damage, and is a practical consideration in managing post-harvest fungal infections.

34) Habitat:
'Habitat' signifies the natural environment where both apple trees and associated pests and pathogens exist. Understanding the habitat is significant for assessing ecological interactions and implications for pest and disease management in apple production.

35) Company:
'Company' may reference agricultural enterprises or academic institutions involved in research and development related to crop management. Collaboration among various companies is essential for advancing agricultural techniques and improving efficacy in managing diseases and pests.

36) Forest:
'Forest' refers to natural ecosystems that may interact with agricultural areas. The proximity of orchard habitats to forest environments influences pest dynamics, disease spread, and can have implications for sustainable orchard management practices.

37) Horn:
'Horn' could reference a specific technical or biological term within the study context, possibly concerning morphological structures or features relevant to plant systems or pests. Additional context is required to clarify its significance fully.

38) Pur:
'Poor' describes conditions that negatively impact agricultural productivity, including inadequate pest management and disease control. Addressing poor management practices is essential for improving crop resilience, maximizing yields, and promoting sustainable growth in apple orchards.

39) Cynodon dactylon:
Cynodon dactylon, also known as Bermuda grass, refers to a common weed in apple orchards. Understanding the competitive dynamics between weeds and crops like apples is significant for effective weed management and ensuring optimal growth conditions for apple trees.

40) Cosmopolitan:
'Cosmopolitan' describes a species or organism that is widespread or found in diverse regions. The term applies to Colletotrichum, highlighting its adaptability and relevance across various geographical locations, thus emphasizing the need for localized management strategies in different environments.

41) Surrounding:
'Surrounding' indicates adjacent areas to apple orchards, which may influence pest and pathogen dynamics. Understanding surrounding ecosystems is important for recognizing how environmental factors and biodiversity impact agricultural production and crop health.

42) Pesticide:
'Pesticide' refers to chemical substances used to control pests and pathogens affecting crops. In the context of apple production, the strategic application of pesticides is crucial for managing disease outbreaks like bitter rot while balancing safety and ecological impact.

43) Srinagar:
Srinagar is a major city in Kashmir known for its agriculture, particularly apple production. Its significance in the study relates to the unique environmental conditions that impact the prevalence of diseases like bitter rot, thereby influencing agricultural practices in the region.

44) Attacked:
'Attacked' describes the harm or damage inflicted upon apple trees and fruits by pests or pathogens. This term emphasizes the vulnerability of crops and underscores the need for effective management strategies to mitigate losses.

45) Worry (Worried, Worrying):
'Worrying' captures the concern surrounding the impact of pesticides on local wildlife. The implications of agricultural practices on ecological health raise important discussions about sustainable farming and the responsibility to protect both crops and natural habitats.

46) Cutting:
'Cutting' may refer to the practice of pruning apple trees or the harvesting of fruits. Proper cutting techniques are vital for maintaining plant health and optimizing yield while also playing a role in disease prevention.

47) Mineral:
'Mineral' signifies the essential nutrients and elements that influence plant growth and health. Understanding the mineral composition of soils and fruits is critical for optimizing fertilization techniques and ensuring healthy apple production.

48) Killing (Killed):
'Killing' refers to the lethal impact of pesticides on targeted pests and possibly non-target organisms. This term highlights the importance of responsible pesticide usage to minimize unintended ecological consequences while effectively managing agricultural pests.

49) Family:
'Family' may refer to the classification of plants in botanical terms, such as the Rosaceae family, which includes apples. Understanding plant family relationships helps scientists and farmers identify risks and cultivate resilience against diseases.

50) Nature:
'Nature' encompasses the natural environment in which apple orchards exist, affecting plant health and disease dynamics. Awareness of ecological interactions enhances the ability to develop effective agricultural practices that are sustainable and compatible with natural systems.

51) Reason:
'Reason' denotes the rationale behind choices made in agricultural practices, particularly concerning disease management. Understanding the reasons behind specific actions helps inform better decision-making and the development of efficacious strategies for managing crops.

52) Summer:
'Summer' indicates the season during which apple fruits develop and ripen, also linked with increased humidity and warmth that favor the proliferation of plant diseases. Monitoring seasonal conditions is critical for timely intervention and management of apple diseases.

53) Animal:
'Animal' encompasses various wildlife that may be affected by agricultural practices or that interact with apple orchards. Understanding animal interactions is important for developing integrated pest management strategies that protect crops while conserving local fauna.

54) Farmer:
'Farmer' refers to the individual or entity engaged in apple cultivation. Understanding farmer practices, challenges, and knowledge is paramount for developing effective communication and education strategies that promote sustainable agricultural practices.

55) Labour (Labor):
'Labor' indicates the essential human work required in orchard management, cultivation, and harvesting. Agricultural labor dynamics inform strategies for improving efficiency, reducing costs, and addressing labor shortages in the farming industry.

56) Indian:
'Indian' denotes the nationality or regional identifier relating to agricultural practices in India, particularly concerning apple production in states like Kashmir. Recognizing local agricultural dynamics is essential for framing context-specific solutions to farming challenges.

57) Botany:
'Botany' covers the scientific study of plants and their biology. Its relevance to the research is in understanding the plant-pathogen relationships, contributing to the scientific knowledge necessary for sustainable horticultural practices and effective disease management.

58) Sugar:
'Sugar' refers to the natural sweeteners present in apple fruits that can influence marketability and consumer preference. Understanding sugar content and its implications for fruit quality enhances strategies for crop selection and marketing.

59) Table:
'Table' may refer to tabulated data or statistical representations arising from the study. Tables provide clear, concise ways to visualize research findings, facilitating analysis and discussion of results in the context of disease management strategies.

60) Food:
'Food' pertains to apples as a significant food source both locally and globally. Understanding the implications of fruit quality and safety directly impacts consumer health, market demand, and food security strategies.

61) Sign:
'Sign' highlights indicators of disease presence or severity in apple crops. Recognizing signs of fruit rot is crucial for timely intervention and effective disease management, ensuring healthier crops and sustained agricultural productivity.

62) Rich (Rch):
'Rich' describes the fertile conditions of Kashmir Valley that promote successful apple cultivation. The richness of the soil and environment contributes to notable agricultural outputs, including high-quality apples, thus emphasizing the region's horticultural significance.

63) Fire:
'Fire' can refer to the natural occurrence or its metaphorical use in discussing agricultural management. Understanding fire's ecological role might relate to pest management strategies or conservation efforts surrounding apple orchards and their environments.

64) Worm:
'Worm' may indicate specific pests affecting apple crops, like fruit worms, which can damage the fruit. Understanding the impact of such pests on crop health fosters better integrated pest management strategies to protect yields.

65) Post:
'Post' could refer to post-harvest management strategies that influence fruit quality and marketability post-harvest. Understanding post-harvest conditions is crucial for minimizing waste and maximizing profits in apple production.

66) Soil:
'Soil' is a fundamental aspect of agriculture impacting plant health and crop yield. Understanding soil composition, quality, and management informs effective practices that enhance apple growth and disease resistance.

67) Dish (Dis):
'Dish' relates to culinary uses of apples, underscoring the importance of fruit quality for consumer satisfaction. Understanding the market demand for processed apple products reinforces the significance of effective disease management to ensure quality.

Other Science Concepts:

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Discover the significance of concepts within the article: ‘Characterization of Colletotrichum species in Kashmir apple orchards’. Further sources in the context of Science might help you critically compare this page with similair documents:

Symptoms and signs, Environmental factor, Disease management, Genetic Diversity, Economic losses, Molecular Taxonomy, Chemical group.

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