Antioxidant activity of hypericum triquetrifolium turra methanol extract
Journal name: World Journal of Pharmaceutical Research
Original article title: Antioxidant activity of hypericum triquetrifolium turra methanol extract
The WJPR includes peer-reviewed publications such as scientific research papers, reports, review articles, company news, thesis reports and case studies in areas of Biology, Pharmaceutical industries and Chemical technology while incorporating ancient fields of knowledge such combining Ayurveda with scientific data.
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Khulood W. Abbood, Ruqaia M. Ibraheem and Ali H. Ad'hiah
World Journal of Pharmaceutical Research:
(An ISO 9001:2015 Certified International Journal)
Full text available for: Antioxidant activity of hypericum triquetrifolium turra methanol extract
Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research
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Summary of article contents:
Introduction
Hypericum triquetrifolium Turra, a medicinal plant from Eastern Europe and the Mediterranean region, has historically been utilized for various therapeutic applications, including sedative, anti-inflammatory, and anti-septic effects. This study aimed to explore the plant's potential as an antioxidant by determining its total flavonoid content, reductive ability, and capacity to scavenge free radicals. The findings revealed that the methanol extract of H. triquetrifolium is rich in flavonoids, which contribute to its strong antioxidant properties.
Flavonoids and Their Health Benefits
Flavonoids are polyphenolic compounds widely recognized for their potential health benefits, particularly in the prevention of cardiovascular diseases and certain cancers. As effective free radical scavengers and inhibitors of lipid peroxidation, flavonoids play a critical role in protecting the body against oxidative stress. The study highlighted that H. triquetrifolium contains 115.73 ± 5.65 µg/ml of flavonoids, showcasing its potential as a natural source of antioxidants that can enhance overall health by mitigating the effects of reactive oxygen species (ROS).
Antioxidant Activity Assessment
The antioxidant activity of H. triquetrifolium was evaluated using the DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging assay. The methanol extract demonstrated significant DPPH scavenging activity across various concentrations, outperforming vitamin C, a known antioxidant. Specifically, at concentrations of 0.250 mg/ml and 0.500 mg/ml, the extract exhibited an impressive radical scavenging activity of 80.00 ± 2.00% and 80.66 ± 1.15%, respectively, indicating its effectiveness in neutralizing free radicals and highlighting its potential therapeutic applications.
Reductive Ability of the Extract
The reductive ability of the methanol extract was examined through a specific assay, revealing that H. triquetrifolium exhibited a higher reductive capacity compared to Trolox (vitamin E) across all tested concentrations. The results showed a concentration-dependent increase in absorbance, with the maximum observed at 0.64 mg/ml, signaling a robust ability to reduce oxidized intermediates in lipid peroxidation. This further confirms the extract's potential as a significant dietary antioxidant.
Conclusion
The study underscores the medicinal potential of Hypericum triquetrifolium as a rich source of flavonoids with notable antioxidant properties. The methanol extract demonstrated both significant DPPH radical scavenging activity and a greater reductive ability compared to established antioxidants like vitamin C and Trolox. These findings suggest that H. triquetrifolium could play a vital role in enhancing health and preventing oxidative damage, validating its traditional use in herbal medicine for a wide range of therapeutic applications.
FAQ section (important questions/answers):
What is Hypericum triquetrifolium traditionally used for?
Hypericum triquetrifolium is traditionally used for its sedative, anti-helminthic, anti-inflammatory, and anti-septic effects. It has potential therapeutic uses for treating burns, gastroenteritis, and possesses anti-nociceptive and anti-oxidant properties.
What was the primary aim of the study?
The study aimed to determine the total flavonoid content and reductive ability of Hypericum triquetrifolium methanol extract, as well as to assess its anti-oxidant activity based on the DPPH radical scavenging effect.
What method was used to prepare the plant extract?
The methanol extract was prepared by extracting 50 grams of the powdered plant material with 250 ml of 80% methanol at 65°C for 3 hours using a soxhlet apparatus.
How was the total flavonoid content determined?
Total flavonoid content was spectrophotometrically determined using the aluminium chloride colorimetric method, with rutin as a standard. The extract showed a flavonoid content of 115.73 ± 5.65 µg/ml.
What does the DPPH assay measure?
The DPPH assay measures the radical scavenging activity of a substance by assessing its ability to reduce the stable DPPH free radical, indicating its potential anti-oxidant activity.
What conclusions were drawn about Hypericum triquetrifolium's anti-oxidant activity?
The methanol extract of Hypericum triquetrifolium was found to be an effective anti-oxidant, demonstrating significant DPPH radical scavenging activity and reductive ability, attributed to its high flavonoid content.
Glossary definitions and references:
Scientific and Ayurvedic Glossary list for “Antioxidant activity of hypericum triquetrifolium turra methanol extract”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.
1) Flavonoid:
Flavonoids are a class of polyphenolic compounds that are abundant in many plants. They are known for their antioxidant properties, which help protect the body against oxidative stress and free radicals. The presence of flavonoids in Hypericum triquetrifolium contributes to its medicinal uses, particularly in treating diseases related to oxidative damage.
2) Activity:
In this context, 'activity' refers to the biological functions and effects that compounds or extracts possess, particularly their ability to scavenge free radicals and demonstrate antioxidant properties. The antioxidant activity of Hypericum triquetrifolium is significant for its potential therapeutic applications and health benefits.
3) Disease:
The text discusses various diseases, including cancer and cardiovascular issues, that are linked to oxidative stress. The antioxidant activity of plant extracts, like those from Hypericum triquetrifolium, can potentially mitigate the effects of such diseases, making them relevant in herbal medicine and preventive health strategies.
4) Species:
Species refers to specific types of plants or organisms studied for their unique properties and effects. Hypericum triquetrifolium is investigated as a species for its medicinal properties, particularly its rich flavonoid content, which enhances its potential usefulness as a natural remedy in traditional medicine.
5) Science (Scientific):
Science encompasses the systematic study of the natural world, including the investigation of medicinal plants. The study of Hypericum triquetrifolium's chemical makeup and its biological effects employs scientific methods to validate its uses in traditional medicine, thus bridging the gap between folklore and scientific inquiry.
6) Table:
The tables in the study present structured data regarding the experimental results, such as total flavonoid content and antioxidant activity across different concentrations. These tables allow for easy comparison and interpretation of the results, facilitating understanding of the potency and efficacy of Hypericum triquetrifolium extracts.
7) Substance:
Substance refers to any material that has a definite composition and can be characterized by its chemical properties. In the context of the study, the methanol extract from Hypericum triquetrifolium is a substance that contains various bioactive compounds, including flavonoids, contributing to its therapeutic potential.
8) Cancer:
Cancer is a major disease characterized by uncontrolled cell growth and is linked to oxidative stress among other factors. The antioxidant properties of plant extracts, particularly flavonoids, can play a crucial role in preventing or managing cancer, highlighting the significance of Hypericum triquetrifolium's constituents.
9) Medicine:
Medicine refers to the science and practice of diagnosing, treating, and preventing illness. The use of Hypericum triquetrifolium in traditional and modern medicine underscores the importance of plant-derived compounds in health care. Its proven antioxidant activity positions it as a valuable medicinal agent.
10) Water:
Water plays a crucial role in the extraction process of bioactive compounds from plant materials. The extraction method described for Hypericum triquetrifolium utilizes methanol and water, which help solvate and isolate flavonoids and other beneficial constituents essential for evaluating the plant's medicinal properties.
11) Study (Studying):
The study aims to investigate the antioxidant and reductive properties of Hypericum triquetrifolium, providing scientific evidence for its traditional uses. This systematic examination contributes to the broader understanding of medicinal plants and their efficacy, validating the importance of traditional knowledge through empirical research.
12) Rich (Rch):
Rich in this context refers to the high concentration of beneficial compounds, particularly flavonoids, in Hypericum triquetrifolium. The richness of these compounds is critical for its potential health benefits, emphasizing the significance of this plant as a valuable resource in herbal medicine.
13) Burning (Burn, Burned, Burnt):
Burn refers to injuries that can benefit from plant extracts with anti-inflammatory properties. Traditional uses of Hypericum triquetrifolium include treating burns, supported by its antioxidant and therapeutic activities that may aid in healing and protecting skin tissue from oxidative damage.
14) Human body:
The human body has natural mechanisms to combat oxidative stress through enzymatic and non-enzymatic defenses. The antioxidant activity of Hypericum triquetrifolium can enhance these defenses, offering protective effects against diseases related to oxidative damage and promoting overall health.
15) Measurement:
Measurement pertains to the quantification of the flavonoid content and antioxidant activity in the plant extract, which is vital for scientific validation. Accurate measurement techniques allow researchers to assess the efficacy and potential therapeutic applications of Hypericum triquetrifolium.
16) Discussion:
The discussion section interprets the findings of the study, contextualizing the results within existing literature. It highlights the implications of Hypericum triquetrifolium's antioxidant properties, suggesting how such findings could influence future research and its applications in healthcare.
17) Developing:
Developing refers to the countries or regions where reliance on traditional plant-based medicines is prevalent. The study of Hypericum triquetrifolium holds relevance for developing nations, where access to affordable healthcare solutions derived from local medicinal plants is crucial.
18) Mountain:
Mountain indicates the geographic origin of Hypericum triquetrifolium, which is collected from mountainous regions. The environment may influence the concentration of bioactive compounds, and understanding the plant's natural habitat is essential for its cultivation and conservation.
19) Richness:
Richness pertains to the abundance and variety of bioactive compounds found within Hypericum triquetrifolium. This richness is significant for its pharmaceutical potential, as diverse compounds may work synergistically to enhance its therapeutic effects and efficacy in treating various health conditions.
20) Quality:
Quality in this context refers to the effectiveness and potency of the methanol extract of Hypericum triquetrifolium, primarily measured by its antioxidant activity and flavonoid content. High-quality extracts are essential for ensuring reliable health benefits and therapeutic applications.
21) Powder:
Powder refers to the form in which the aerial parts of Hypericum triquetrifolium are processed for extraction. The powdered plant material is essential for solubilizing active compounds in methanol, making it easier to extract the flavonoids and other medicinal constituents that contribute to the plant's health benefits.
22) Drug:
Drug in this context refers to the potential pharmaceutical applications of Hypericum triquetrifolium due to its bioactive compounds. The plant's extracts may act as natural drugs with therapeutic properties, especially in treating oxidative stress-related diseases, mirroring the functionality of synthetic pharmaceuticals.
23) Life:
Life refers to the overall health and well-being of individuals. Antioxidants like those found in Hypericum triquetrifolium can play a critical role in enhancing the quality of life by preventing diseases and promoting better health outcomes, thus illustrating the importance of dietary and medicinal plants.
Other Science Concepts:
Discover the significance of concepts within the article: ‘Antioxidant activity of hypericum triquetrifolium turra methanol extract’. Further sources in the context of Science might help you critically compare this page with similair documents:
Medicinal plant, Comparative study, Statistical analysis, Medical condition, Significant difference, Antioxidant activity, Bioavailability, Antioxidant properties, Oxidative stress, Reactive oxygen species, Anti oxidant activity, Cognitive impairment, Biological Activity, Anti-inflammatory effect, Aqueous extract, Antioxidant capacity, Antioxidant activities, Flavonoid content, Reactive oxygen species (ROS), Reducing power assay, Total flavonoid content, DPPH free radical, DPPH radical scavenging activity, Gastroenteritis treatment, Fatty Acid Composition, Medicinal application, Radical scavenging, Free radical scavenger, Phenolic compound, Cardiovascular prevention, Polyphenolic compound, Dietary flavonoid, Natural anti oxidant, Food Source, Virgin olive oil.