Anti-inflammatory effects of Opuntia ficus-indica and Punica granatum oils.
Journal name: World Journal of Pharmaceutical Research
Original article title: Anti-inflammatory activity of seed oils of opuntia ficus-indica l. and punica granatum l. from morocco
The WJPR includes peer-reviewed publications such as scientific research papers, reports, review articles, company news, thesis reports and case studies in areas of Biology, Pharmaceutical industries and Chemical technology while incorporating ancient fields of knowledge such combining Ayurveda with scientific data.
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Fadoua El Hachimi, Ghizlane Hajjaj, Abdenbi Bendriss, Yahia Cherrah and Katim Alaoui
World Journal of Pharmaceutical Research:
(An ISO 9001:2015 Certified International Journal)
Full text available for: Anti-inflammatory activity of seed oils of opuntia ficus-indica l. and punica granatum l. from morocco
Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research
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Summary of article contents:
Introduction
The study investigates the anti-inflammatory properties of seed oils derived from two plants, Opuntia ficus-indica (prickly pear) and Punica granatum (pomegranate), which are commonly utilized in Moroccan traditional medicine. The research aims to evaluate the safety and efficacy of these oils through various experimental models, with specific attention to their potential applications in pharmaceuticals, nutraceuticals, and cosmeceuticals.
Acute Toxicity Assessment
An acute toxicity study was conducted according to the OECD guidelines, involving twelve female Swiss mice administered a single oral dose of 5000 mg/kg of both seed oils. The results revealed no mortality or significant behavioral changes in the test subjects over the 14-day observation period. These findings indicate that both Opuntia ficus-indica and Punica granatum seed oils possess a high safety profile and can be considered safe for long-term use.
Anti-inflammatory Effects in Carrageenan-Induced Oedema
The anti-inflammatory activity of the seed oils was evaluated using carrageenan-induced paw oedema in Wistar rats. The oils were administered at doses of 200 mg/kg and 300 mg/kg prior to the carrageenan injection. The results demonstrated a statistically significant reduction in paw oedema compared to the control group, with the oils showing inhibition percentages comparable to that of the reference drug indomethacin. This suggests that both seed oils exhibit substantial anti-inflammatory effects through their ability to modulate inflammatory responses.
Experimental Trauma-Induced Oedema Analysis
Further analysis was performed using an experimental trauma-induced model of rat paw oedema. Similar to the carrageenan-induced model, both seed oils effectively reduced paw swelling at doses of 200 mg/kg and 300 mg/kg. The maximal reductions were observed at 6 hours post-treatment, with the Opuntia ficus-indica seed oil showing a reduction of 87.52% and the Punica granatum seed oil demonstrating an 88.36% decrease in inflammation. These results reinforce the antinociceptive properties of both oils, signaling their relevance in managing inflammatory conditions.
Conclusion
The study concludes that the seed oils of Opuntia ficus-indica and Punica granatum exhibit significant anti-inflammatory and safe characteristics, supporting their traditional use in medicinal practices in Morocco. The results underline the need for further research to identify the specific bioactive compounds responsible for these effects and to explore their mechanisms of action. Given the growing interest in natural products for health benefits, these findings could pave the way for developing new therapeutic agents targeting inflammation and pain management.
FAQ section (important questions/answers):
What is the main purpose of the study?
The study aims to evaluate the anti-inflammatory activity of seed oils from Opuntia ficus-indica and Punica granatum to enhance their use in traditional Moroccan medicine.
How was the oil extracted from the seeds?
The oil was extracted using a soxhlet apparatus, where the seeds were pressed, and the extract was concentrated under reduced pressure before being stored in a refrigerator.
What methods were used to test anti-inflammatory activity?
The anti-inflammatory activity was evaluated using carrageenan-induced and experimental trauma-induced paw oedema models in Wistar rats.
What were the results of the acute toxicity study?
The acute toxicity study indicated that the seed oils did not produce mortality or adverse behavioral changes in mice, suggesting they are safe for long-term administration.
What significant effects did the seed oils have?
Both seed oils demonstrated significant anti-inflammatory effects, comparable to the reference drug Indomethacin in both carrageenan-induced and trauma-induced inflammation models.
What does the conclusion suggest about the seed oils?
The conclusion suggests that the seed oils from Opuntia ficus-indica and Punica granatum have effective anti-inflammatory properties, supporting their usage in folk medicine but highlighting the need for further studies.
Glossary definitions and references:
Scientific and Ayurvedic Glossary list for “Anti-inflammatory effects of Opuntia ficus-indica and Punica granatum oils.”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.
1) Edema (Oedema):
Edema is often interchangeably used with oedema, indicating swelling due to fluid accumulation in tissues. Studying edema is crucial in understanding inflammatory conditions, and therefore, researching compounds that can address this issue is central to developing effective treatments.
2) Punica granatum:
Punica granatum, commonly known as pomegranate, is a fruit-bearing tree known for its rich antioxidant properties and health benefits. Its seeds and oils have been utilized in traditional medicine for their anti-inflammatory effects. This study focuses on extracting and evaluating the benefits of its seed oils.
3) Animal:
Animal refers to non-human living beings used in scientific research, particularly in preclinical studies to evaluate drug efficacy and safety. In this context, animals such as Swiss mice and Wistar rats were used to test the anti-inflammatory properties of seed oils from Opuntia and Punica granatum.
4) Activity:
Activity, in this study, pertains to the biological effects of substances, particularly the anti-inflammatory effects of seed oils. Evaluating the activity of these oils in experimental animals helps in understanding their potential clinical applications and guides future research in natural medicinal compounds.
5) Drug:
A drug is a chemical substance used to prevent, diagnose, treat, or alleviate symptoms of diseases. The study compares the anti-inflammatory activity of seed oils to standard reference drugs like indomethacin, providing insights into their potential benefits as natural therapeutic agents.
6) Inflammation:
Inflammation is a biological response to harmful stimuli, characterized by redness, swelling, heat, and pain. It plays a default protective role but can become chronic, leading to various diseases. This research focuses on natural products that can mitigate inflammation effectively, highlighting their role in traditional medicine.
7) Study (Studying):
Study designates the systematic investigation conducted to answer a specific research question. Here, it describes the assessment of the anti-inflammatory activity and safety of seed oils from prickly pear and pomegranate. The outcomes enhance understanding and application of these plants in both traditional and modern healthcare.
8) Hind:
Hind specifies the rear part, in this research, referring to the hind paws of rats where oedema is measured post-injection of carrageenan to induce inflammation. The hind paw oedema model is a standard for evaluating the efficacy of anti-inflammatory agents in experimental settings.
9) Toxicity:
Toxicity indicates the degree to which a substance can harm living organisms. Evaluating the toxicity of the seed oils is essential to ensure their safety for consumption and therapeutic use. This study conducted acute toxicity tests to confirm that no adverse effects occurred at high doses.
10) Medicine:
Medicine is a field focused on the prevention, diagnosis, and treatment of diseases. The study evaluates the medicinal properties of Opuntia ficus-indica and Punica granatum, underscoring their potential role in improving health and well-being. Such research contributes to the innovations within herbal medicine.
11) Water:
Water is a critical component in biological systems, necessary for life. In this research, water is mentioned in the context of controlled pre-experimental fasting for animals and as a solvent for the substances used in testing, emphasizing its ubiquitous role in scientific experimentation.
12) Table:
Table refers to a structured presentation of data, summarizing results from the experiments conducted. Tables in the study organize and communicate findings related to the efficacy of seed oils on inflammation, aiding clarity and comparison against control and reference drugs.
13) Pomegranate:
Pomegranate, or Punica granatum, possesses deep cultural and medicinal significance and is known for its health benefits. Its extracts have been the subject of numerous studies due to their rich content of anti-inflammatory compounds. Understanding its properties contributes to a deeper knowledge of herbal pharmacology.
14) Hand:
Hand appears in the context of measurements taken in the experiment and serves as a metaphorical reference to manual tasks in research. While not a primary focus, physical interaction with study specimen or equipment is implicit in methodological processes conducted during the research.
15) Relative:
Relative pertains to comparisons made in the study, specifically regarding the efficacy of the seed oils against standard drugs. The term emphasizes the significance of assessing the performance of proposed treatments in relation to established pharmacological substances to validate their effectiveness.
16) Pain:
Pain is an unpleasant sensory experience associated with actual or potential tissue damage or described in terms of such damage. The study's context on anti-inflammatory activity inherently ties to pain management, as inflammation often leads to heightened pain responses in biological systems.
17) Science (Scientific):
Science is the systematic pursuit of knowledge through observation and experimentation. This study represents an intersection of various scientific disciplines, including botany, pharmacology, and toxicology, aimed at exploring the therapeutic potential of natural products for health management.
18) Family:
Family in biological taxonomy is a classification used to group related species. Opuntia and Punica belong to different families, Cactaceae and Punicaceae respectively. Understanding family relationships aids in the study of phytochemistry and determining the potential properties of the plants in question.
19) Food:
Food plays a crucial role in providing essential nutrients for health and well-being. The researched plants, particularly Opuntia, are consumed as food in many cultures and have additional health benefits explored in this study, linking nutrition with herbal medicine.
20) Pharmacological:
Pharmacological relates to the effects and mechanisms of drugs and bioactive compounds in the body. The study examines the pharmacological activities of the seed oils from the plants under review, assessing their potential use as natural anti-inflammatory treatments.
21) Pharmacology:
Pharmacology is the branch of medicine that studies the effects and mechanisms of drugs. This study contributes to pharmacological knowledge by evaluating the anti-inflammatory effects and safety of oils derived from natural sources, aiming to integrate herbal remedies into clinical applications.
22) Accumulation (Accumulating, Accumulate):
Accumulation refers to the build-up of substances within biological systems. In the context of this study, it relates to the accumulation of fluid leading to oedema during inflammation, emphasizing the need for evaluating compounds that can manage or reduce this condition effectively.
23) Agriculture:
Agriculture is the practice of cultivating plants for food, fiber, and other products. Understanding the role of plants like Opuntia and Punica granatum in agriculture highlights their significance not only in traditional diets but also in potential pharmaceutical applications derived from their agricultural use.
24) Discussion:
Discussion serves as a critical analysis in research, where findings are interpreted in the context of existing literature. This section evaluates and connects the results of the anti-inflammatory activities of seed oils, providing insight for future studies and understanding implications for their use.
25) Toxicology:
Toxicology is the study of the adverse effects of substances on living organisms. This research incorporates toxicological assessments to ensure the safety of seed oils extracted from plants, confirming that they are free from harmful effects at tested dosages.
26) Substance:
Substance refers to any specific matter with a defined composition and properties. In this study, different substances include the extracted seed oils and the chemicals used for inducing inflammation in rats, crucial for understanding their interactions and biological effects.
27) Container:
Container denotes receptacles used to store samples or substances. In the study, appropriate storage of seed oils in containers ensures their stability and preserves their properties when analyzed for potential medicinal benefits, facilitating effective experimental conditions.
28) Himalaya (Hima-alaya):
Himalaya refers to a mountainous region known for its diverse flora, including medicinal plants. While not the primary focus, mentioning the Himalayan connection in the study alludes to the geographical diversity of punica granatum cultivation, accentuating its widespread relevance.
29) Swelling:
Swelling is a visible symptom of inflammation resulting from oedema. In this study, swelling is quantitatively measured to assess the efficacy of anti-inflammatory treatments. Understanding the mechanisms behind swelling is vital for developing effective therapeutic interventions in inflammatory conditions.
30) Pregnant:
Pregnant refers to female animals in a state of carrying offspring, an essential factor when considering the safety of treatments within animal studies. Assessments in research often factor in pregnancy as a variable influencing the outcomes, ensuring safety for all demographics.
31) Species:
Species signifies distinct groups of organisms that can interbreed. In this study, the species of interest are Opuntia ficus-indica and Punica granatum. Understanding the characteristics of these species provides insights into their unique properties and potential therapeutic uses.
32) Field:
Field denotes the area of study or the experimental site where plant materials are cultivated. In this study, reference to the experimental field signifies controlled conditions under which plants were harvested, supporting the credibility of research outcomes stemming from them.
33) Blood:
Blood is vital for transporting nutrients throughout the body. In terms of inflammation, blood flow increases to affected areas, leading to redness and swelling. Investigating how therapeutic agents modify blood dynamics aids in understanding their efficacy in treating inflammatory disorders.
34) Tree:
Tree generally refers to a large perennial plant. In the context of this study, it relates to the growth form of pomegranate, highlighting its long-living nature and the cultural significance it holds in various regions as a source of food and medicinal products.
35) Diet:
Diet encompasses the types of food consumed by organisms. Healthy diets potentially incorporate foods rich in therapeutic properties, such as those derived from Opuntia and Punica granatum, encouraging lifestyle choices aimed at health maintenance and prevention of chronic diseases.
36) Sign:
Sign signifies an indicator or symptom of a condition. In the context of inflammation, signs like swelling, redness, and pain guide the diagnosis and treatment plans in clinical practice. Understanding these signs paves the way for more effective therapeutic strategies.
37) Cage:
Cage refers to the enclosures used for housing animals in research settings. In this study, cages provided controlled environments for the animals, ensuring their well-being during experimentation and guiding appropriate methodologies to obtain reliable results.
Other Science Concepts:
Discover the significance of concepts within the article: ‘Anti-inflammatory effects of Opuntia ficus-indica and Punica granatum oils.’. Further sources in the context of Science might help you critically compare this page with similair documents:
Herbal remedies, Traditional medicine, Anti-inflammatory activity, Acute Toxicity Studies, Animal model, Indomethacin, Significant anti-inflammatory activity, Carrageenan-induced rat paw oedema.