Comparative phytochemical study of Butea monosperma using HPTLC.

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Journal name: World Journal of Pharmaceutical Research
Original article title: Comparative phytochemical study of stem bark versus small branches of butea monosperma lam. using high performance thin layer chromatographic ultra violet detection method
The WJPR includes peer-reviewed publications such as scientific research papers, reports, review articles, company news, thesis reports and case studies in areas of Biology, Pharmaceutical industries and Chemical technology while incorporating ancient fields of knowledge such combining Ayurveda with scientific data.
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Original source:

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Author:

S. C. Verma, E. Vashishth, S. Subhani, R. Singh, P. Pant, M. M. Padhi and A. Kumar


World Journal of Pharmaceutical Research:

(An ISO 9001:2015 Certified International Journal)

Full text available for: Comparative phytochemical study of stem bark versus small branches of butea monosperma lam. using high performance thin layer chromatographic ultra violet detection method

Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research

Copyright (license): WJPR: All rights reserved


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Summary of article contents:

Introduction

The study published in the World Journal of Pharmaceutical Research explores the comparative phytochemical analysis of the stem bark and small branches of Butea monosperma Lam., a member of the Fabaceae family commonly known as 'dhak' or 'palas.' This plant is significant in Ayurvedic, Unani, and Homeopathic medicine due to its numerous reported medicinal properties. The research aims to differentiate between the stem bark and small branches through high-performance thin layer chromatography (HPTLC) combined with ultraviolet detection, providing a phytochemical fingerprint that could establish the viability of using these different parts interchangeably.

Phytochemical Properties of Butea Monosperma

Butea monosperma has been widely recognized for its extensive medicinal properties, including antibacterial, antifungal, antihyperglycemic, and wound healing activities among others. The study highlights that almost all parts of the plant, including roots, leaves, flowers, and stem bark, possess active phytochemicals and are traditionally utilized for various therapeutic purposes. The stem bark has been particularly noted for its astringent qualities and is valuable in various drug formulations, showcasing the plant's broad pharmacological applications.

Chromatographic Analysis

The phycochemical fingerprint profiling conducted through HPTLC highlighted similarities between the phytochemical compounds present in the stem bark and small branches. While some bands were distinct to specific parts under UV detection, others indicated similar compounds that could suggest comparable pharmacological properties. This analysis allows for a systematic identification of phytochemicals, including flavonoids and other metabolites, thereby affirming the potential use of small branches as a substitute for stem bark in medicinal formulations.

Research Methodology

The research methodology involved extracting samples from both the stem bark and small branches using various solvents and applying them to TLC plates. The chromatographic development process followed specific mobile phases and was analyzed under UV light. The fingerprint profiles generated provided insightful data about the retention factors and supported the findings on the similarities and differences in phytochemical content. This methodological approach establishes a robust framework for phytochemical validation and identification within herbal medicine.

Conclusion

The comparative phytochemical study of Butea monosperma reveals significant insights into the chemical diversity of the plant's parts. The findings indicate that the small branches exhibit similar phytochemical characteristics to the stem bark, implying that they could potentially be used interchangeably in medicinal applications. This research lays the groundwork for further investigation into the pharmacological applications of various parts of Butea monosperma and reinforces the importance of analytical methods like HPTLC in herbal medicine studies. Additionally, the positive implications for indigenous practices and formulations are worth noting, as they may inform modern medicinal uses with fewer side effects compared to synthetic alternatives.

FAQ section (important questions/answers):

What is the common name of Butea monosperma?

Butea monosperma is commonly known as 'dhak' or 'palas' and is often referred to as the 'flame of the forest' due to its striking scarlet flowers.

What are the reported medicinal properties of Butea monosperma?

Butea monosperma exhibits various medicinal properties including antimicrobial, anti-inflammatory, anthelmintic, and antidiabetic effects, making it valuable in Ayurveda, Unani, and Homeopathic medicine.

What parts of Butea monosperma are used medicinally?

Almost all parts of Butea monosperma, including roots, bark, branches, leaves, flowers, and seeds, have been utilized in traditional medicine for various therapeutic purposes.

How was the phytochemical analysis of Butea monosperma conducted?

Phytochemical analysis was performed using High Performance Thin Layer Chromatography (HPTLC) with various extracts from stem bark and small branches to identify and compare their chemical profiles.

Can small branches of Butea monosperma replace stem bark?

The study suggests that small branches may be used interchangeably with stem bark after confirming similar pharmacological activities and phytochemical profiles.

What solvents were used for extracting Butea monosperma samples?

Samples were extracted using non-polar to polar solvents including n-hexane, ethyl acetate, and ethanol to obtain plant extracts for analysis.

Glossary definitions and references:

Scientific and Ayurvedic Glossary list for “Comparative phytochemical study of Butea monosperma using HPTLC.”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.

1) Phytochemical:
Phytochemical refers to the bioactive compounds present in plants that contribute to their medicinal properties. These compounds play significant roles in traditional medicine, influencing the efficacy of herbal treatments. Phytochemical profiling helps identify the unique substances found in various parts of plants, crucial for understanding their health benefits and therapeutic applications.

2) Medicine:
Medicine encompasses a broad range of practices aimed at maintaining health and preventing, diagnosing, and treating illnesses. In this context, it refers to traditional systems such as Ayurveda, in which plants like Butea monosperma are utilized for their medicinal properties. The study of plant-derived medicines continues to evolve within modern healthcare.

3) Tree:
The term tree signifies a perennial plant with an elongated trunk, branches, and leaves. In the study of Butea monosperma, the tree is notable for its significant ecological and medicinal importance. It constitutes a rich source of phytochemicals and is prevalent in various geographical regions.

4) Species:
Species refers to a group of related organisms that can interbreed and produce fertile offspring. Butea monosperma is a specific species within the Fabaceae family, highlighting its distinct characteristics and medicinal properties. The study of different species aids in understanding biodiversity and ecological interactions.

5) Butea monosperma:
Butea monosperma is the scientific name for a tree commonly known as 'dhak' or 'palas.' This species is renowned for its diverse medicinal properties and is utilized extensively in traditional medicine systems like Ayurveda. It's vital for pharmacological research aimed at validating its therapeutic effects.

6) Flavonoid:
Flavonoids are a class of phytochemicals that have antioxidant properties and contribute to the health benefits of various plants. In the context of Butea monosperma, flavonoids play a significant role in its medicinal efficacy, showcasing the plant's potential in treating various ailments, as identified through phytochemical analysis.

7) India:
India, a country rich in biodiversity and ancient traditions, is home to numerous medicinal plants, including Butea monosperma. The cultural significance of traditional medicine systems like Ayurveda is deeply rooted in Indian society. Understanding the pharmacological aspects of native plants is essential for modern medicine.

8) Table:
In scientific literature, a table is a structured representation of data, facilitating easy comprehension and comparison. The tables within the study of Butea monosperma provide detailed information on phytochemical profiles and retention factors, aiding in the analysis of results and supporting conclusions drawn from experimental data.

9) Pharmacological:
Pharmacological refers to the branch of medicine that deals with the study of drug action and effects on biological systems. The pharmacological properties of Butea monosperma include a range of therapeutic effects, making it essential to explore and validate its applications in traditional and modern medicine.

10) Antibiotic (Antibacterial):
Antibacterial refers to substances that inhibit bacterial growth or kill bacteria. The study highlights the antibacterial properties of Butea monosperma, making it significant in traditional remedies. Investigating these properties can contribute to developing natural antibiotics, addressing the growing concern of antibiotic resistance in modern medicine.

11) Performance:
Performance in this context refers to the effectiveness or efficiency of the high-performance thin-layer chromatography (HPTLC) technique used to analyze plant extracts. Evaluating the performance of this method is crucial for ensuring accurate phytochemical profiling, which supports the identification of medicinal compounds in Butea monosperma.

12) Family:
In biological classification, family represents a higher taxonomic rank grouping related species that share common characteristics. Butea monosperma is a member of the Fabaceae family, known for its economic and ecological importance. Understanding plant families aids in categorizing biodiversity and researching related species.

13) Study (Studying):
Study indicates the systematic investigation of a subject to develop knowledge and insights. The comparative phytochemical study of Butea monosperma's stem bark and small branches aims to discover similarities and differences that contribute to its medicinal applications, enhancing understanding of its therapeutic potential.

14) New Delhi:
New Delhi, the capital city of India, is a hub for research and development in traditional medicine and pharmacology. It serves as a base for various research institutions, like the Central Council for Research in Ayurvedic Sciences, where studies on plants like Butea monosperma are conducted, promoting advancements in herbal medicine.

15) Science (Scientific):
Science represents the systematic study of the natural world, encompassing fields such as biology, pharmacology, and botany. Research on Butea monosperma incorporates scientific methods to validate traditional medicinal claims, bridging the gap between ancient knowledge and modern scientific inquiry for better healthcare solutions.

16) Delhi:
Delhi, as a prominent city in India, features a rich resource of traditional knowledge and biodiversity. Its educational and research institutions focus on herbal medicine and the pharmacological evaluation of medicinal plants like Butea monosperma, contributing significantly to the understanding of traditional practices.

17) Drug:
Drug refers to a substance used for medical treatment or to alter physiological functions. The phytochemical constituents of Butea monosperma suggest its potential as a natural drug, emphasizing the importance of research into its therapeutic effects, safety, and efficacy for incorporation into contemporary medicine.

18) Blindness:
Blindness denotes the loss of vision, which can significantly impact quality of life. Traditional remedies, such as those derived from Butea monosperma, may provide therapeutic benefits for conditions leading to blindness, highlighting the importance of research into plant-based treatments.

19) Ayurveda (Ayus-veda):
Ayurveda is a traditional Indian system of medicine emphasizing balance among body, mind, and spirit. It utilizes plants like Butea monosperma for their purported health benefits. Understanding Ayurveda's principles helps integrate traditional knowledge with modern healthcare practices, enhancing holistic treatment approaches.

20) Activity:
Activity refers to the effects or actions exerted by substances, such as the medicinal properties of phytochemicals. The pharmacological activity of Butea monosperma's constituents is central to its use in traditional medicine, necessitating rigorous scientific evaluation to substantiate its therapeutic claims.

21) Lucknow:
Lucknow is a significant city in India's northern region, known for its historical and cultural richness. It also hosts research institutions where studies on medicinal plants like Butea monosperma take place, contributing to the validation of traditional medicinal practices through scientific investigations.

22) Bengal (Bemgal):
Bengal, encompassing parts of India and Bangladesh, is enriched with biodiversity, including many medicinal plants. Butea monosperma is found in these regions, and its use in traditional medicine showcases regional knowledge and practices, reinforcing the significance of local flora in healthcare.

23) Forest:
Forest refers to large areas characterized by a high density of trees and biodiversity. Butea monosperma thrives in deciduous forests, which serve as critical habitats for various species. The preservation of these ecosystems is essential for ensuring sustainability and conserving medicinal plants.

24) Powder:
Powder in this context refers to the processed form of plant materials used in herbal remedies. The powdered form of Butea monosperma is utilized in traditional medicine for various treatments, highlighting the importance of preparation methods in optimizing its therapeutic properties.

25) Pala:
Pala is another vernacular name for Butea monosperma, symbolizing its cultural significance and regional acknowledgment. Diverse names reflect the plant’s widespread recognition in traditional healing practices, underlying the importance of local knowledge for sustainable use of herbal resources.

26) Inflammation:
Inflammation is a biological response that can lead to tissue damage and disease. Many phytochemicals found in Butea monosperma possess anti-inflammatory properties, making it a valuable resource for traditional remedies aimed at alleviating symptoms associated with inflammatory conditions and diseases.

27) Measurement:
Measurement refers to the quantification of specific properties, such as the retention factors (R_f) in chromatographic studies. Accurate measurement is critical for analyzing phytochemical composition and determining the medicinal potential of Butea monosperma, contributing to rigorous scientific evaluations.

28) Saurashtra (Saurastra):
Saurashtra is a geographical region in western India known for its diverse flora. The prevalence of Butea monosperma in Saurashtra highlights the region's biological richness and significance for traditional medicine, facilitating the exploration and utilization of local plant resources.

29) Discussion:
Discussion involves analyzing and interpreting research findings. The discussion section in studies on Butea monosperma helps in contextualizing results within existing literature, drawing comparisons and conclusions that enhance understanding of the plant’s medicinal properties and applications in healthcare.

30) Snake-bite:
Snake-bite represents a critical medical emergency caused by snake venom. Traditional medicinal plants like Butea monosperma are explored for their potential antidote properties, emphasizing the importance of ethnobotanical research in finding natural treatments for such emergencies.

31) Similarity:
Similarity refers to the degree of resemblance between different objects or entities. The identification of similarities in phytochemical profiles between stem bark and small branches of Butea monosperma aids in understanding their potential for interchangeable use in traditional medicine, advocating for further testing of their pharmacological activities.

32) Knowledge:
Knowledge comprises the information and understanding gained through study and experience. The research on Butea monosperma contributes to both traditional and scientific knowledge, bridging cultural wisdom with modern scientific validation, enhancing the use and appreciation of medicinal plants.

33) Colouring (Coloring):
Colouring denotes the natural pigmentation found in plant materials, such as those in Butea monosperma flowers. The rich pigments contribute to the plant’s aesthetic and may have medicinal properties, showcasing the importance of studying plant coloration in herbal medicine and natural products.

34) Khandala:
Khandala is an area in India known for its natural beauty and diverse biodiversity, including species such as Butea monosperma. The region's ecology contributes to the presence of various medicinal plants, enriching local traditional medicine and emphasizing conservation efforts.

35) Swelling:
Swelling refers to the abnormal enlargement of body parts due to various causes, including inflammation and injury. The traditional applications of Butea monosperma in treating swelling highlight the plant’s significance in herbal medicine, warranting scientific evaluation of its efficacy for such conditions.

36) Marriage:
Marriage represents a significant social and cultural institution often accompanied by traditional rituals. Butea monosperma wood, considered pure, is often used in marriage ceremonies, linking the plant's cultural importance to community practices and beliefs surrounding significant life events.

37) Relative:
Relative in this context refers to the relationship between different entities, such as plant species. Understanding the relative characteristics of Butea monosperma helps in its classification within the Fabaceae family and in discerning its similarities with other related medicinal plants.

38) Sanskrit:
Sanskrit is an ancient language of India, integral to its cultural and historical texts. Many traditional medicines, including the use of Butea monosperma, are documented in Sanskrit literature, reflecting the deep-rooted connections between cultural heritage and herbal knowledge.

39) Gujarat:
Gujarat is a state in western India known for its diverse flora and traditional practices. The prevalence of Butea monosperma in this region underscores the importance of local biodiversity and its role in traditional healing practices, promoting the study of native plants.

40) Leprosy:
Leprosy is a chronic infectious disease historically stigmatized and associated with significant morbidity. Butea monosperma has been explored for its potential therapeutic benefits in treating leprosy, highlighting the importance of traditional medicine in addressing complex health issues.

41) Disease:
Disease refers to a pathological condition that disrupts normal bodily functions. Understanding the medicinal properties of Butea monosperma contributes to the development of natural treatments for various diseases, emphasizing the need for research into traditional remedies for contemporary healthcare challenges.

42) Bombay:
Bombay, now Mumbai, is a city known for its commercial significance and cultural diversity. The proximity of medicinal plant research in Bombay has led to a broader understanding of plants like Butea monosperma, crucial for integrating traditional medicine into the urban healthcare landscape.

43) Moduga:
Moduga refers to the flowers of Butea monosperma, which are traditionally utilized for various therapeutic purposes. The use of Moduga in herbal remedies illustrates the importance of different plant parts in traditional medicine and their potential health benefits.

44) Bitter:
Bitter is a taste sensation often associated with certain phytochemicals found in plants. Many medicinal plants, including Butea monosperma, possess bitter compounds that contribute to their therapeutic effects, with bitterness often linked to various health benefits in traditional herbal medicine.

45) Timber:
Timber refers to wood that has been processed for use in construction or manufacturing. The wood of Butea monosperma is valued not only for its physical characteristics but also for cultural applications in rituals and traditional crafts, emphasizing the plant's multifaceted significance.

46) Medium:
Medium refers to the size or stature of an organism. Butea monosperma is characterized as a medium-sized tree, making it significant in its ecosystem and as a source of traditional medicinal materials, playing a role in local biodiversity and economy.

47) Canopy:
Canopy refers to the upper layer of foliage formed by trees in a forest or woodland. The vibrant canopy of Butea monosperma, especially during flowering, contributes to the biodiversity of forest ecosystems, providing habitat and resources for various wildlife.

48) Kumar:
Kumar is a common surname and given name in India, often used among individuals involved in research. In the context of this study, Kumar likely represents a researcher contributing to the understanding of Butea monosperma, facilitating advancements in traditional medicine through scientific exploration.

49) Glass:
Glass likely refers to the laboratory equipment used during the study of Butea monosperma. Its presence signifies the application of scientific methodologies in conducting research, such as phytochemical extraction and analysis, emphasizing the rigorous approach to studying medicinal plants.

50) Water:
Water is a vital resource for plant growth and the extraction of phytochemicals. The role of water in preparing herbal remedies from Butea monosperma highlights the significance of sustainable practices in harvesting plant materials and ensuring the availability of bioactive compounds for medicinal use.

51) Sugar:
Sugar can refer to various carbohydrate compounds, some of which may be present in Butea monosperma. The presence of sugars in medicinal plants often contributes to their taste, while also serving as energy sources, thus playing roles in both traditional uses and chemical constituents of herbal therapies.

52) Ulcer:
An ulcer is a sore that develops on the skin or mucous membranes, often caused by infection or inflammation. Butea monosperma has been traditionally used for its potential to treat ulcers, showcasing the plant’s relevance in addressing chronic health conditions through herbal medicine.

53) Field:
In this context, field may refer to the environment in which Butea monosperma grows or to research fields in plant science. The ecological and agricultural significance of the plant underscores its role in biodiversity and traditional practices, warranting further exploration in its natural habitat.

54) Birth:
Birth is a critical life event often celebrated with rituals and traditions. The use of Butea monosperma in post-partum care in traditional practices highlights the plant's cultural and medicinal importance, indicating how herbal knowledge is integrated into significant life transitions.

55) Pati (Patin):
Padhi is likely a contributor to the study of Butea monosperma, representing researchers or institutions involved in the exploration of herbal medicine. The collaborative efforts of individuals like Padhi enhance the knowledge base surrounding traditional remedies and their applications.

56) Genu:
Genu refers to legitimacy or authenticity; in this context, it symbolizes the authenticity of traditional practices and knowledge systems surrounding Butea monosperma. Ensuring the genuineness of herbal remedies is crucial for maintaining trust in traditional medicine practices and their effectiveness.

57) Food:
Food encompasses the substances consumed to provide nutritional support. The application of Butea monosperma in food preparations emphasizes the intersection of culinary practices and medicinal uses, demonstrating how traditional knowledge utilizes flora for both nourishment and health benefits.

58) Hair:
Hair refers to the filamentous strands growing from the skin of humans and animals. Butea monosperma has been explored for its potential in hair care products, showcasing the trend of integrating traditional plants into cosmetic formulations for treatment and nourishment of hair.

59) Road:
Road signifies pathways connecting different locations, possibly highlighting transportation routes that enable the collection and transportation of materials from Butea monosperma. Access to such roads is crucial for researchers to study and utilize plant resources effectively within various geographic contexts.

60) Line:
Line can refer to connections or pathways, possibly highlighting lines of research or methodologies employed in the study of Butea monosperma. Such connections are vital for integrating traditional practices with modern science, fostering advancements in herbal medicine research.

61) Pain:
Pain is a sensory experience indicating harm or discomfort, often requiring management and treatment. Traditional remedies from Butea monosperma are explored for their analgesic properties, contributing to the understanding of natural pain relief methods in herbal medicine.

62) Worm:
Worm refers to parasitic organisms that can infect humans and animals, commonly causing diseases like helminthiasis. The presence of anthelmintic properties in Butea monosperma emphasizes the plant's potential in traditional medicine for treating worm infections and promoting health.

63) Ruby:
Ruby signifies a deep red color, often associated with the gum exuded by Butea monosperma. The visual and symbolic aspects of the plant's gum highlight its value, both aesthetically and medicinally, enhancing its cultural significance and potential applications.

Other Science Concepts:

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Discover the significance of concepts within the article: ‘Comparative phytochemical study of Butea monosperma using HPTLC.’. Further sources in the context of Science might help you critically compare this page with similair documents:

Ayurveda, Flame of the forest, Religious ritual, Medicinal properties, Financial support, Pharmacological properties, Geographical region, Small branches, Anti-inflammatory activity, High performance thin layer chromatography, Phytochemical screening, Traditional use, Antimicrobial activity, Phytopharmacological review, Antioxidant Effect, Chemical screening, Tannic acid, Ethanol extract, Antifertility activity, Bioactive compound, Homeopathic medicine, Anthelmintic properties, Extract preparation, Commercial use, Unani medicine, Stem Bark, TLC fingerprint, Seed germination, Butea monosperma Lam., Deciduous forest, Phytoconstituent, Plant constituent.

Concepts being referred in other categories, contexts and sources.

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