Antioxidant activity of curry leaf extracts in cisplatin Wistar rats

| Posted in: Science

Journal name: World Journal of Pharmaceutical Research
Original article title: Evaluation on antioxidant activity of curry leaf extracts in cisplatin induced wistar rats
The WJPR includes peer-reviewed publications such as scientific research papers, reports, review articles, company news, thesis reports and case studies in areas of Biology, Pharmaceutical industries and Chemical technology while incorporating ancient fields of knowledge such combining Ayurveda with scientific data.
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Original source:

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Author:

Balasubramanian Sathyamurthy, Ganesh Dama, Arul Balasubramanian and Surya Narayana Vvs


World Journal of Pharmaceutical Research:

(An ISO 9001:2015 Certified International Journal)

Full text available for: Evaluation on antioxidant activity of curry leaf extracts in cisplatin induced wistar rats

Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research

Copyright (license): WJPR: All rights reserved


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Summary of article contents:

Introduction

The study outlined in the article evaluates the antioxidant activity of curry leaf extracts in Wistar rats, particularly focusing on oxidative stress induced by cisplatin, a chemotherapeutic agent used to treat various cancers. Reactive oxygen species (ROS) are harmful byproducts of cellular metabolism that contribute to oxidative stress, leading to conditions such as cancer, aging, and heart disease. The research highlights the role of antioxidant enzymes in mitigating these damaging effects and explores the potential of curry leaves, known for their antioxidative and cholesterol-reducing properties, to enhance antioxidant activity in the body.

The Role of Reactive Oxygen Species

Reactive oxygen species (ROS) are produced continuously in cells and can cause significant cellular damage if not properly managed by antioxidants. This oxidative stress results when there is an imbalance between ROS production and antioxidant defense mechanisms. Cisplatin, while effective in treating certain cancers, induces oxidative stress leading to multiorgan toxicity and damage to cellular components such as DNA. The article discusses how the body utilizes antioxidants like glutathione and various enzymes to counteract the harmful effects of ROS, emphasizing the importance of maintaining this balance to prevent biological damage.

Impact of Cisplatin on Antioxidant Levels

Cisplatin treatment has been shown to significantly decrease the levels of crucial antioxidants like glutathione in treated rats, indicating its role in depleting the body's antioxidant defenses. The study found that while cisplatin reduced serum glutathione, the administration of curry leaf extract helped maintain glutathione levels, suggesting its protective role against oxidative stress. The net loss of body protein and the associated weight loss observed in the cisplatin-treated group pointed to the compound's detrimental impact, reinforcing the need for effective antioxidants during cancer treatments.

Benefits of Curry Leaf Extracts

Curry leaves were specifically noted for their ability to reduce lipid peroxidation and support antioxidant enzyme activity in the presence of cisplatin. The study demonstrated that the incorporation of curry leaf extracts not only helped lower lipid peroxides but also maintained levels of critical antioxidant enzymes like superoxide dismutase, glutathione reductase, and catalase, which are essential for detoxifying harmful substances. This suggests that curry leaves can counteract the adverse effects of oxidative stress, providing a potential dietary measure to enhance overall health during chemotherapy.

Conclusion

The overall findings of the study suggest that curry leaves exhibit significant antioxidant activity that could effectively protect against oxidative stress induced by cisplatin. The inclusion of curry leaves in the diet may offer health benefits, especially in patients undergoing cancer treatment. It is recommended to incorporate curry leaves in their powdered form for optimal antioxidant effects, as the study indicates that their cooking method can affect their potential. Future research should explore the precise mechanisms by which curry leaves exert their antioxidative properties and evaluate their efficacy in broader clinical contexts.

FAQ section (important questions/answers):

What is the main focus of the study on curry leaves?

The study evaluates the antioxidant activity of curry leaf extracts in Wistar rats induced with oxidative stress by cisplatin, assessing potential protective effects.

How does oxidative stress impact health according to the research?

Oxidative stress, caused by reactive oxygen species (ROS), can lead to various health issues, including cancer, aging, and heart diseases by damaging cellular components.

What role does glutathione play in the body?

Glutathione is a key antioxidant that helps maintain redox equilibrium in cells, detoxifies harmful substances, and regulates various cellular functions, essential for maintaining health.

What were the observed effects of cisplatin on body weight?

Cisplatin treatment resulted in a significant reduction in body weight in the Wistar rats, highlighting its impact on muscle protein degradation through induced oxidative stress.

What findings did the study report regarding curry leaves' antioxidant effects?

Curry leaves showed significant antioxidant properties, preventing oxidative stress and maintaining levels of important antioxidant enzymes in the presence of cisplatin.

What is the recommended way to include curry leaves in the diet?

The study suggests using curry leaves in dry powder form in food preparation, as this may enhance their antioxidant potential compared to fresh usage.

Glossary definitions and references:

Scientific and Ayurvedic Glossary list for “Antioxidant activity of curry leaf extracts in cisplatin Wistar rats”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.

1) Narayana (Nara-ayana, Nara-yana):
Narayana is a name that appears in the context of principals and notable figures in the research. It represents the individuals involved in the study and reflects their expertise in the fields of biochemistry and pharmacology, which are critical to understanding the interactions and effects of curry leaf extracts on oxidative stress.

2) Activity:
Activity refers to the effectiveness or potency of substances, such as curry leaf extracts, in exhibiting antioxidant properties. In this context, researchers assess the biochemical activity related to cellular protection against harmful reactive oxygen species, underscoring its importance in combatting diseases like cancer and other oxidative stress-related conditions.

3) Surya (Shurya):
Surya is part of the name of one of the researchers involved in the study. This name often evokes connotations of the sun, light, and energy, symbolizing the aim of the research to shed light on the benefits of natural substances like curry leaves in health and disease mitigation.

4) Animal:
Animal studies, particularly involving Wistar rats in this research, are crucial for understanding the physiological effects of treatments in a controlled environment. They help bridge the gap between in vitro findings and human applications, providing insights into how curry leaf extracts can influence oxidative stress within living organisms.

5) Blood:
Blood is a critical fluid in living organisms that transports nutrients, oxygen, and waste. The study measures various parameters in the blood to evaluate how treatments with curry leaf extracts influence oxidative stress and antioxidant levels, reflecting the overall health impact of dietary substances on systemic health.

6) Water:
Water is the medium used for preparing the aqueous curry leaf extract in this research. It is essential for solubilizing the bioactive compounds in curry leaves, allowing for the assessment of their pharmacological properties and efficacy in combating oxidative stress and promoting health in the studied animal models.

7) Study (Studying):
The study is a systematic investigation focusing on evaluating the antioxidant activity of curry leaf extracts. It aims to provide empirical evidence on their potential health benefits, particularly concerning oxidative stress, thus contributing to the broader knowledge in pharmaceutical research and potential therapeutic applications.

8) Species:
In this context, species refers to the biological classification of living organisms, specifically the Wistar rats used in the research. Understanding species-specific responses is vital for interpreting the results and making predictions about how similar treatments may affect human health based on animal models.

9) Cancer:
Cancer is a major health concern addressed in this research. The study investigates the protective role of curry leaf extracts against oxidative stress linked to cancer therapies, particularly analyzing how these extracts may mitigate the harmful effects of cisplatin, a common chemotherapeutic agent.

10) India:
India is the country of origin for the curry leaves being studied, as well as the institution where the research was conducted. The cultural and geographical context of India plays a significant role in the use of curry leaves in traditional medicine, enhancing the relevance of this study.

11) Diet:
Diet encompasses the nutritional habits and food consumption patterns. In this study, the effect of curry leaf extracts on oxidative stress and overall health in the context of diet demonstrates the importance of including natural antioxidants in daily meals for improved health outcomes.

12) Toxicity:
Toxicity refers to the adverse effects of substances on living organisms. This study evaluates the toxicity associated with cisplatin, a chemotherapy drug, and how curry leaf extracts may alleviate some harmful effects, establishing a link between dietary components and reducing toxicity in treatments.

13) Disease:
Disease refers to pathological conditions that impair normal functioning. This research emphasizes the role of antioxidants from curry leaf extracts in combating oxidative stress, which is linked to various diseases, thereby suggesting dietary interventions as a preventive strategy for disease management.

14) Food:
Food is a core element of the study, highlighting the significance of dietary antioxidants. The research suggests that incorporating curry leaves into everyday meals may provide health benefits, particularly in reducing oxidative damage, thus promoting a preventive approach to health through diet.

15) Substance:
Substance refers to the specific materials being studied, such as curry leaf extracts, which contain bioactive compounds. Understanding the properties of these substances is essential for assessing their impact on health and their potential as therapeutic agents in preventing oxidative stress.

16) Reva:
Reva is associated with REVA University, the institution where researchers are affiliated. The university represents a center of learning and research that contributes to advancing knowledge, particularly in biochemistry and pharmacology, which are crucial for this study on curry leaves.

17) Pharmacology:
Pharmacology is the branch of medicine that focuses on drugs and their effects on living systems. The research connects biochemical principles with pharmacological impacts, examining how curry leaf extracts can modify oxidative stress responses, informing future therapeutic strategies.

18) Inflammation:
Inflammation is a biological response to harmful stimuli and is often linked to oxidative stress. This study indirectly addresses inflammation by exploring how antioxidants from curry leaves can mitigate oxidative damage, potentially influencing inflammation-related diseases and health outcomes.

19) Observation:
Observation refers to the process of monitoring and recording findings during the research study. It is crucial for drawing conclusions about the effects of curry leaf extracts on oxidative stress markers and provides empirical data that enriches scientific discourse in the field.

20) Discussion:
Discussion is a critical component of research, interpreting the results obtained from the study. It allows for an analysis of how findings relate to existing knowledge, facilitating a deeper understanding of the significance of curry leaf extracts in health-related contexts.

21) Tamilnadu (Tamil-nadu):
Tamil Nadu is a state in India, reinforcing the cultural context of the study. This geographical identifier reflects the traditional uses of curry leaves in local cuisine and medicine, thereby emphasizing the relevance of the research to regional dietary practices.

22) Vinayaka:
Vinayaka is part of the name associated with Vinayaka Mission's College of Pharmacy, where some researchers are affiliated. This connection highlights the collaboration and contribution of academic institutions to advancing research on natural remedies and pharmacological benefits.

23) Relative:
Relative refers to the relationship between different parameters measured in the study, such as the relative levels of antioxidants in the blood. This context of relativity assists in understanding changes in physiological responses to treatments with curry leaf extracts.

24) Science (Scientific):
Science denotes the systematic study of phenomena based on empirical evidence. The research exemplifies scientific inquiry into the antioxidant properties of curry leaves, aiming to contribute to the broader understanding of natural compounds in health and disease.

25) Repair:
Repair refers to the cellular processes that restore damage caused by oxidative stress. The study's focus on how curry leaf extracts may support cellular repair mechanisms underscores their potential therapeutic value in preventing damage related to various health conditions.

26) Powder:
Powder indicates the form in which curry leaves may be consumed for health benefits. The research suggests that curry leaves should ideally be used in powdered form for maximizing their antioxidant effects, rather than as a garnish, impacting dietary recommendations.

27) Cotton:
Cotton refers to the material used for filtering the curry leaf extract during the preparation process. This practical aspect highlights the methods employed in the research, ensuring that the extracts analyzed are free from impurities, essential for accurate scientific assessments.

28) Indian:
Indian refers to the cultural and geographical context of the study. This term emphasizes the traditional significance of curry leaves in Indian cuisine and herbal medicine, enhancing the study's relevance to local dietary practices and health remedies.

29) Shalem (Salem):
Salem is a city in India associated with Vinayaka Mission's College of Pharmacy. This location is relevant as it reflects the region's engagement in pharmaceutical research and education, contributing to the investigation of natural substances like curry leaves.

30) Nandi (Nandin, Namdi):
Nandi is a researcher associated with the study. This name represents the contribution of individual scientists to the understanding of antioxidant properties and health benefits derived from natural extracts, which is crucial for advancing pharmacological knowledge.

31) House:
House refers to the animal house where Wistar rats were maintained during the study. Proper housing conditions are crucial for ensuring the well-being of research subjects and for providing reliable, reproducible data that contributes to the validity of study findings.

32) Tamil:
Tamil refers to the linguistic and cultural heritage of a region in India where curry leaves are prominent. It highlights the significance of understanding local practices and the role of traditional medicine in shaping contemporary pharmacological research.

33) Dama:
Dama is another researcher involved in the study. The inclusion of multiple researchers underscores the collaborative nature of scientific investigation, where collective expertise contributes to insights on the antioxidant activity of curry leaf extracts.

34) Aruli (Arul):
Arul is a name associated with one of the researchers in this study. The presence of various researchers indicates a multi-disciplinary approach to understanding the properties of natural antioxidants and their applications in health sciences.

35) Nadu:
Nadu, as part of Tamil Nadu, signifies the regional context of the study. It highlights the cultural and agricultural importance of curry leaves in local diets, thereby contextualizing the research's focus on natural health remedies in this geographic area.

36) Line:
Line refers to a lineage of scientific inquiry. In the context of this research, it pertains to the explorations into the antioxidant properties of natural substances, leading to potential therapeutic interventions based on cumulative scientific evidence.

37) Post:
Post indicates the follow-up or subsequent investigations after initial findings. In this research, it represents the potential for future studies that could expand upon the current knowledge of curry leaves and their effects on human health and diseases.

38) Drug:
Drug refers to chemical substances used to treat diseases. This study connects traditional herbal extracts with modern pharmacology, suggesting that natural substances like curry leaves may have properties that can complement or enhance the efficacy of established drugs, such as cisplatin.

39) Male:
Male signifies the sex of the Wistar rats used in the study. This detail is important in research design as physiological differences between sexes can influence experimental results and outcomes in the context of drug efficacy and antioxidant activity.

40) Life:
Life denotes living organisms, and in this context, it refers to the cellular and biochemical processes studied in rats. The research aims to improve the understanding of how natural antioxidants impact life through their protection against oxidative stress and related health issues.

Other Science Concepts:

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Discover the significance of concepts within the article: ‘Antioxidant activity of curry leaf extracts in cisplatin Wistar rats’. Further sources in the context of Science might help you critically compare this page with similair documents:

Dosage administration, Antioxidant activity, Oxidative stress, Animal model, Reactive oxygen species, Lipid peroxidation, Aqueous extract, Body weight, Superoxide dismutase, Catalase, Lipid peroxide, Cytotoxicity, Catalase activity, Serum analysis, Glutathione, Free Radical, Serum, Glutathione reductase, Curry leaves, Antioxidant enzyme, Wistar rat, Normal saline solution, Glutathione level, Whole blood, Superoxide dismutase level.

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