Antibacterial efficacy of Ocimum tenuiflorum vs. Plectranthus amboinicus.

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Journal name: World Journal of Pharmaceutical Research
Original article title: Comparison of antibacterial activity of ocimum tenuiflorum l.and plectranthus amboinicus (lour.) spreng against the clinical pathogens staphylococcus aureus, pseudomonas aeruginosa and escherichia-coli
The WJPR includes peer-reviewed publications such as scientific research papers, reports, review articles, company news, thesis reports and case studies in areas of Biology, Pharmaceutical industries and Chemical technology while incorporating ancient fields of knowledge such combining Ayurveda with scientific data.
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Original source:

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Author:

Jiyauddin K., Samer A. D., Darashhni T., Rasha S., Jawad A., M. Kaleemullah, S. Budiasih, Rasny M. R., M. Qamar, Hamid K., Sakina R., Junainah A. H., Fadli A. and Eddy Y.


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Full text available for: Comparison of antibacterial activity of ocimum tenuiflorum l.and plectranthus amboinicus (lour.) spreng against the clinical pathogens staphylococcus aureus, pseudomonas aeruginosa and escherichia-coli

Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research

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Summary of article contents:

Introduction

This study, conducted by Jiyauddin et al., aims to compare the antibacterial activities of two medicinal plants, Ocimum tenuiflorum (commonly known as Holy Basil) and Plectranthus amboinicus (also known as Cuban Oregano), against several clinical pathogens, specifically Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli. The research was prompted by the increasing rates of infections caused by these bacteria and the corresponding resistance to conventional antibiotics. The study's methodology involved creating ethanolic extracts from the leaves of both plants and evaluating their antimicrobial properties through disc diffusion methods.

Significant Antibacterial Activity of Plectranthus amboinicus

The findings of the study revealed that Plectranthus amboinicus exhibited greater antibacterial potency compared to Ocimum tenuiflorum. At a concentration of 100 mg/mL, the extracts of P. amboinicus showed maximum inhibition zones of 14 mm against S. aureus, 12 mm against P. aeruginosa, and 10 mm against E. coli. These results were notably higher than those of O. tenuiflorum, which recorded inhibition zones of 12 mm against S. aureus and 10 mm against both P. aeruginosa and E. coli. This indicates that the leaf extracts of Plectranthus amboinicus may potentially serve as a more effective natural antibacterial agent against certain gram-positive and gram-negative pathogens.

Resistance Observed in Escherichia coli

Interestingly, the study also highlighted that both plant extracts showed resistance against E. coli, with the highest inhibition zone recorded being only 10 mm even at the highest concentration of 100 mg/mL. This suggests that while Plectranthus amboinicus has more potent antibacterial effects overall, its effectiveness is limited against E. coli. The resistance observed is significant, indicating a need for further research into how these extracts can be modified or combined with other treatments to enhance their efficacy against resistant strains of bacteria.

Antibacterial Variation Between Gram-Positive and Gram-Negative Bacteria

The results of this study demonstrated a clear distinction in antibacterial efficacy against gram-positive versus gram-negative bacteria. Both Ocimum tenuiflorum and Plectranthus amboinicus exhibited higher inhibitory effects on gram-positive S. aureus compared to the gram-negative pathogens, Pseudomonas aeruginosa and Escherichia coli. This disparity may be attributed to the structural differences in bacterial cell walls, with gram-negative bacteria posing a greater barrier to the penetration of phytochemicals found in these plant extracts. The study's findings underscore the necessity of targeting gram-positive infections with these extracts while exploring other solutions for gram-negative bacterial challenges.

Conclusion

In conclusion, the study by Jiyauddin et al. substantiates the potential of Ocimum tenuiflorum and Plectranthus amboinicus as natural antibacterial agents, particularly emphasizing Plectranthus amboinicus for its stronger activity against Staphylococcus aureus. However, both plants demonstrated limited effectiveness against Escherichia coli, suggesting the presence of resistant strains that warrant further investigation. The observed patterns of antibacterial activity raise critical insights into the development of naturally derived therapeutics, highlighting the importance of understanding the unique interactions between plant-derived compounds and various bacterial pathogens in the pursuit of effective treatment strategies.

FAQ section (important questions/answers):

What was the objective of this study on herbal extracts?

The objective was to identify and compare the antibacterial activity of *Ocimum tenuiflorum* and *Plectranthus amboinicus* leaves against selected gram-positive and gram-negative bacteria.

Which bacteria were tested against the herbal extracts?

The study tested the antibacterial activity against *Staphylococcus aureus*, *Pseudomonas aeruginosa*, and *Escherichia coli*.

What method was used to test antibacterial activity?

The antimicrobial activity of the extracts was determined using the disc diffusion method, measuring the zone of inhibition for each bacterial strain.

What were the results of *Plectranthus amboinicus* extracts?

*Plectranthus amboinicus* exhibited higher antibacterial activity, especially against *S. aureus*, with maximum inhibition zones observed at higher concentrations.

Did *Ocimum tenuiflorum* show significant antibacterial activity?

Yes, *Ocimum tenuiflorum* also displayed antibacterial activity but was less effective than *Plectranthus amboinicus*, particularly against *P. aeruginosa* and *E. coli*.

How were the plant extracts prepared for testing?

The leaves were collected, dried, ground into powder, and then extracted using dehydrated ethanol, followed by filtration and evaporation to concentrate the extract.

Glossary definitions and references:

Scientific and Ayurvedic Glossary list for “Antibacterial efficacy of Ocimum tenuiflorum vs. Plectranthus amboinicus.”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.

1) Activity:
In the context of the study, 'activity' refers to the biological efficacy of plant extracts in inhibiting bacterial growth. Understanding and measuring the activity of antimicrobial agents like plant extracts is crucial for determining their potential as natural alternatives to conventional antibiotics in treating bacterial infections and other diseases.

2) Aureus:
'Aureus' specifically refers to Staphylococcus aureus, a Gram-positive bacterium commonly responsible for various infections, including skin infections and respiratory illnesses. The study focuses on the antibacterial effects of plant extracts against S. aureus, highlighting their potential use in managing infections caused by this pathogen.

3) Antibiotic (Antibacterial):
'Antibiotic' refers to substances used to treat bacterial infections. The study aims to identify natural products with antibiotic-like properties from plant extracts, providing insights into alternatives for conventional antibiotics and contributing to addressing antibiotic resistance issues prevalent in modern medicine.

4) Study (Studying):
'Study' denotes the systematic investigation carried out to compare the antibacterial properties of Ocimum tenuiflorum and Plectranthus amboinicus. It encompasses the research methodology, results, and analysis aimed at discovering potential natural antimicrobial agents that could serve as alternatives to synthetic antibiotics.

5) Science (Scientific):
'Science' encompasses the systematic body of knowledge that forms the basis of the presented study. It refers to a methodological approach to investigating natural phenomena, ensuring that findings concerning plant extracts’ antibacterial properties are based on evidence, replicability, and adherence to scientific principles.

6) Drug:
In the context of the study, 'drug' refers to substances used for medical treatment, particularly for their antibacterial properties. The research underscores the significance of identifying new drugs derived from natural sources, like plants, to combat antibiotic resistance and improve healthcare outcomes.

7) Disease:
'Disease' pertains to disorders caused by pathogens, such as bacteria, leading to various health complications in humans. The study emphasizes the relevance of botanical remedies in treating bacterial infections and diseases, contributing to understanding how natural products can support modern medical practices.

8) Alam (Alaṁ):
'Alam' translates to 'nature' in Malay. In this research, it signifies the natural environments where the studied plants grow. Understanding the habitats of these plants is crucial for their collection and evaluation for medicinal properties, as environmental factors can influence their bioactive compound profiles.

9) Sah:
'Shah' can refer to Shah Alam, a city in Malaysia where the research took place, specifically at the Management and Science University. The location underscores the significance of local flora in traditional medicine practices, finding connections between indigenous plants and modern scientific research.

10) Surface:
In the microbiological context, 'surface' refers to the area of the culture medium where bacteria grow and interact with the extracts. The study’s antimicrobial activity assessment relies on examining how plant extracts inhibit bacteria on the agar surface, thus determining their zone of inhibition.

11) Table:
'Table' denotes a systematic arrangement of data, often used for summarizing the results, such as the antibacterial zone of inhibition measurements. In this study, tables help in organizing findings, making it easier to compare the antibacterial effects of different plant extracts at varying concentrations.

12) Water:
'Water' is a solvent used in the extraction processes of the study. Its role in dilution and extraction techniques is fundamental to isolating the bioactive compounds from the plant materials, which are crucial for assessing their antibacterial effectiveness against the selected pathogens.

13) Life:
'Life' relates to the biological aspects discussed in the study, particularly concerning human health. The antibacterial properties of the plant extracts significantly contribute to enhancing life quality by providing natural alternatives to combat infections, highlighting the importance of sustaining health through natural remedies.

14) Ocimum sanctum:
'Ocimum sanctum', commonly known as holy basil or tulsi, is a medicinal plant explored in the study alongside Ocimum tenuiflorum. This species is renowned for its pharmacological properties, including its antibacterial effects, making it relevant for potential therapeutic applications in combating infections.

15) Medicine:
'Medicine' pertains to the science of diagnosing, treating, and preventing diseases. This study is relevant to the field of medicine as it explores the role of plant-derived antibacterial agents in therapeutic applications, advocating for the integration of traditional knowledge and modern scientific approaches.

16) Family:
'Family' refers to the taxonomic classification of the studied plants within the Lamiaceae family. Understanding plant taxonomy is crucial for identifying related species with potential medicinal properties, contributing to the broader research on plant-based antimicrobial agents and their applications in healthcare.

17) Powder:
'Powder' refers to the processed form of plant leaves used in the study. The grinding of dried leaves into powder is a necessary step in preparing the extracts, facilitating the extraction of active compounds for subsequent antibacterial activity tests against the selected pathogens.

18) Genu:
'Genu' refers to the classification or genu of the studied plants, indicating the genus level in the taxonomic hierarchy. Proper classification of plants is vital in pharmacological studies as it helps in understanding their properties and potential applications in medicine based on genus-related characteristics.

19) Phytochemical:
'Phytochemical' indicates the bioactive compounds derived from plants that can exert therapeutic effects. The study closely examines phytochemicals in Ocimum tenuiflorum and Plectranthus amboinicus, highlighting their importance in producing plant extracts with significant antibacterial activity and potential health benefits.

20) Observation:
'Observation' pertains to the process of watching and recording the antimicrobial effects of plant extracts during the study. Observations provide empirical evidence of the antibacterial activity of the studied plants, contributing to the analysis and interpretation of research findings.

21) Discussion:
'Discussion' is a critical component of the study where results are interpreted, compared with prior research, and implications are drawn. It addresses the significance of findings within the context of existing literature, emphasizing how the research contributes to understanding plant-based antibacterial activities.

22) Developing:
'Developing' refers to the progress in discovering new antimicrobial agents, especially in the context of pharmaceutical developments from natural sources. The study aims to highlight the potential of plant extracts as innovative solutions in combating bacterial infections in the face of emerging antibiotic resistance.

23) Container:
'Container' signifies the vessels used for storing the plant extracts during preparation. This aspect is crucial in ensuring proper handling, preservation, and avoiding contamination, which is fundamental in maintaining the integrity of the extracts for reliable results in the study.

24) Flavonoid:
'Flavonoid' refers to a class of phytochemicals known for their antioxidant and antimicrobial properties. The study may investigate the presence of flavonoids in the selected plants, providing insights into their contribution to the antibacterial activity observed, thus helping to understand their therapeutic potential.

25) Toxicity:
'Toxicity' refers to the harmful effects substances may have on organisms. The study highlights the importance of evaluating toxicity levels of the plant extracts, ensuring that potential antibacterial agents are safe for use in therapeutic applications without causing adverse effects on human health.

26) Habitat:
'Habitat' refers to the natural environment where the studied plants grow. Understanding the habitat conditions can influence the botanical and biochemical properties of the plants, informing researchers about the best practices for cultivation, collection, and potential applications in medicine.

27) Species:
'Species' denotes specific classifications of living organisms, essential for documenting the two plants studied—Ocimum tenuiflorum and Plectranthus amboinicus. Accurate identification of species is pivotal in research, ensuring precise communication about the plants’ properties and uses, particularly in medicinal contexts.

28) Account:
'Account' refers to a narrative or report explaining the findings in the study. Providing an account of the research results is crucial for disseminating knowledge, engaging with the scientific community, and contributing to the broader discourse on natural antimicrobial agents derived from plants.

29) Medium:
'Medium' refers to the growth environment for the bacterial cultures used in testing antibacterial activity. The choice of medium is essential for the growth of specific bacteria, allowing the researchers to accurately assess the effectiveness of the plant extracts against the pathogens.

30) Animal:
'Animal' signifies living organisms other than plants that may also serve as subjects in related studies. The connection between plant-based remedies and animal health can extend to veterinary medicine and insights into how natural products can benefit broader biological health.

31) Cotton:
'Cotton' refers to the material used (such as cotton swabs) for transferring and applying bacterial inoculates during the study's experimental procedures. The use of sterile cotton swabs is essential for maintaining aseptic conditions during microbiological testing, ensuring reliable results.

32) Putra:
'Putra' refers to Universiti Putra Malaysia, where the study's plant specimens were authenticated. Acknowledging the role of academic institutions in scientific research underscores the collaboration between various entities, enhancing reliability and credibility in the identification and study of medicinal plants.

33) Rasa (Rasha):
'Rasha' is likely a reference to one of the authors or contributors involved in the study. Acknowledging authorship is crucial in academic research, providing credit for contributions to the research and ensuring transparency in scientific discourse.

34) Jaya:
'Jaya' refers to the geographic locality of Petaling Jaya in Malaysia, where the plants were collected. Understanding local geography and culture around medicinal plants adds depth to the study, emphasizing the role of indigenous knowledge in plant use for health and disease management.

35) Pain:
'Pain' signifies the physical suffering that may result from infections or diseases that the study's plant extracts could potentially alleviate. Highlighting the therapeutic potential of natural remedies in pain management stresses the importance of exploring alternative treatment methods in medicine.

36) Burning (Burn, Burned, Burnt):
'Burn' refers to a type of injury that may occur from thermal exposure. The connection between plant extracts and skin healing properties, including treating burns, reflects the potential therapeutic applications of the studied plants, further promoting the exploration of natural medicine solutions.

37) Moci:
'Mosi' is potentially a reference to a researcher or prior study that contributed to the discourse surrounding the antibacterial activities of the mentioned plants. Recognizing contributions from previous research fosters a collaborative understanding of the ongoing investigations within the scientific community.

Other Science Concepts:

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Discover the significance of concepts within the article: ‘Antibacterial efficacy of Ocimum tenuiflorum vs. Plectranthus amboinicus.’. Further sources in the context of Science might help you critically compare this page with similair documents:

Plectranthus amboinicus, Phytochemical, Research findings, Traditional medicine, Antibacterial activity, Antimicrobial activity, Disc diffusion method, Zone of inhibition, Staphylococcus aureus, Ethanolic extract, Escherichia coli, Pseudomonas aeruginosa, Gram positive bacteria, Gram negative bacteria, Positive control, Ethanol extract, Inhibitory effect, Statistically significant difference, Bioactive compound, Gram negative, E. coli, Sensitivity test, S. aureus, P. aeruginosa, Nutrient agar medium, Two-way ANOVA, Significant antibacterial activity, Two-way analysis of variance, Clinical pathogen, High potential, Previous research, Statistical Package for the Social Science, Herbal Remedie, Leave extract.

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