GC-MS analysis of bioactive compounds in Moringa and Emblica extracts.

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Journal name: World Journal of Pharmaceutical Research
Original article title: Determination of bioactive compounds from the petroleum ether leaf extract of moringa oleifera and phyllanthus emblica using gc-ms analysis
The WJPR includes peer-reviewed publications such as scientific research papers, reports, review articles, company news, thesis reports and case studies in areas of Biology, Pharmaceutical industries and Chemical technology while incorporating ancient fields of knowledge such combining Ayurveda with scientific data.
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Author:

Malliga Elangovan, M.S. Dhanarajan, I. Elangovan


World Journal of Pharmaceutical Research:

(An ISO 9001:2015 Certified International Journal)

Full text available for: Determination of bioactive compounds from the petroleum ether leaf extract of moringa oleifera and phyllanthus emblica using gc-ms analysis

Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research


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Summary of article contents:

Introduction

The study undertaken by Malliga Elangovan et al. aimed to identify and analyze biologically active compounds present in the petroleum ether leaf extracts of two traditional medicinal plants: Moringa oleifera and Phyllanthus emblica. The research utilized advanced analytical techniques such as Gas Chromatography-Mass Spectrometry (GC-MS) and Fourier Transform Infrared Spectroscopy (FT-IR) to uncover the potential therapeutic properties of these plants. These methods are significant in analyzing non-polar components and providing insights into the chemical profiles of plants with traditional medicinal uses.

Phytochemical Analysis Using FT-IR

The FT-IR analysis of both plant extracts indicated the presence of various functional groups associated with bioactive compounds. Specific peaks identified corresponded to O-H alcohols, C-H alkanes, ketones, and esters, revealing a diverse range of phytochemicals. This analysis serves as a qualitative tool, allowing researchers to determine the functional components of the extracts, which may contribute to their medicinal properties. The identification of these groups underlines the complexity and therapeutic potential of both Moringa oleifera and Phyllanthus emblica.

Identification of Bioactive Compounds via GC-MS

The GC-MS analysis revealed a significantly higher number of bioactive compounds in Phyllanthus emblica (17 compounds) compared to Moringa oleifera (8 compounds). Major compounds identified in Moringa oleifera included unsaturated alcohols, Vitamin E, and triterpenoids, while Phyllanthus emblica featured alcohols, fatty acids, and ester compounds. The extraction and identification of these compounds signify their potential therapeutic applications, making them valuable resources for drug development. The study highlights the importance of GC-MS technology in determining the chemical profiles of medicinal plants.

Medicinal Properties of Identified Compounds

The identified bioactive components have been associated with various medicinal activities, underscoring the pharmacological significance of these plants. Key constituents like D.L. α-Tocopherol exhibit antioxidant properties, while others, such as Hentriacontane, have been linked to anti-inflammatory and anticancer activities. These findings support the historical practices of using these plants in traditional medicine and provide scientific validation for their medicinal applications. The exploration of these compounds could lead to the discovery of effective therapeutic agents.

Conclusion

In conclusion, the study confirms that petroleum ether leaf extracts of Moringa oleifera and Phyllanthus emblica are rich sources of bioactive compounds with significant pharmacological effects. The identification of these compounds through sophisticated analytical methods provides a strong foundation for further research into their individual properties and potential applications in the development of herbal medicines. Continued exploration of these plants may contribute to the advancement of natural product chemistry and therapeutic innovation, promoting the use of traditional medicinal knowledge in modern healthcare.

FAQ section (important questions/answers):

What is the objective of the study involving Moringa oleifera and Phyllanthus emblica?

The study aims to determine the presence of bioactive phytoconstituents in the petroleum ether leaf extracts of Moringa oleifera and Phyllanthus emblica using Gas Chromatography-Mass Spectrometry (GC-MS) analysis.

What methods were used to analyze the plant extracts?

The plant extracts were screened using Fourier Transform Infrared Spectroscopy (FT-IR) and Gas Chromatography-Mass Spectrometry (GC-MS) to identify various bioactive compounds and their functional groups.

What were the major findings from the FT-IR analysis?

FT-IR analysis detected several functional groups in both plant extracts, including alcohols, alkanes, amides, and ketones, indicating diverse bioactive chemical compounds present.

How many bioactive compounds were identified in Moringa oleifera and Phyllanthus emblica?

The petroleum ether leaf extract of Moringa oleifera revealed eight bioactive compounds, while Phyllanthus emblica exhibited seventeen bioactive compounds, highlighting their medicinal potential.

What are some bioactive compounds found in Moringa oleifera?

Notable bioactive compounds in Moringa oleifera include Unsaturated alcohols, Saturated hydrocarbons, and Vitamin E, which are associated with various health benefits.

What conclusions were drawn regarding the medicinal potential of these plants?

The study concluded that both Moringa oleifera and Phyllanthus emblica extracts possess valuable bioactive compounds, supporting their traditional use in medicinal applications for various ailments.

Glossary definitions and references:

Scientific and Ayurvedic Glossary list for “GC-MS analysis of bioactive compounds in Moringa and Emblica extracts.”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.

1) Phyllanthus emblica:
Phyllanthus emblica, also known as Amla or Indian Gooseberry, is a medicinal tree from the Euphorbiaceae family. It has been utilized in traditional Ayurvedic medicine for its wide-ranging health benefits, including antioxidant, anti-inflammatory, and antibacterial properties, making it significant in both herbal medicine and nutritional applications.

2) Activity:
Activity, in the context of pharmacology and botany, refers to the biological effects exhibited by a substance, particularly concerning its therapeutic effects. The activity of bioactive compounds derived from plants like Moringa oleifera and Phyllanthus emblica is essential for evaluating their medicinal potential and applications in treating various ailments.

3) Table:
In scientific research, a table presents data systematically and concisely, allowing for easy comparison and analysis. This paper includes numerous tables summarizing relevant findings from GC-MS and FT-IR analyses of plant extracts, illustrating key phytochemicals and their respective concentrations, vital for further medicinal exploration.

4) Study (Studying):
Study denotes a systematic investigation aimed at acquiring knowledge. In this context, the study procured bioactive compounds from the leaf extracts of Phyllanthus emblica and Moringa oleifera using modern analytical techniques like GC-MS, contributing to the understanding of their pharmacological benefits and potential medicinal applications.

5) Pelmo:
PELmo refers to the petroleum ether leaf extract of Moringa oleifera used in the study. The extraction process employs petroleum ether to isolate phytochemicals from the leaves, which are subsequently analyzed for their bioactive properties, emphasizing the efficacy of Moringa in traditional and modern medicine.

6) Antibiotic (Antibacterial):
Antibacterial refers to substances that inhibit bacterial growth or kill bacteria. Many phytochemicals identified in the study exhibit antibacterial activity, thereby supporting the therapeutic use of Phyllanthus emblica and Moringa oleifera in treating bacterial infections, highlighting the plants' significance in herbal medicine.

7) India:
India is a country rich in biodiversity, particularly in medicinal plants. It plays a crucial role in the traditional system of medicine, such as Ayurveda. The study's plants, Moringa oleifera and Phyllanthus emblica, are widely utilized in Indian herbal practices for their health benefits.

8) Beta:
Beta in this context typically refers to beta-amyrin, a triterpene compound found in the leaf extract of Moringa oleifera. Beta-amyrin possesses various pharmacological properties, including anti-inflammatory and antioxidant effects, thereby contributing to the therapeutic potential of the plant in treating health conditions.

9) Drug:
A drug is any substance used in the diagnosis, cure, mitigation, treatment, or prevention of disease. The phytochemicals found in Moringa oleifera and Phyllanthus emblica demonstrate potential as natural drugs, reinforcing the importance of these plants in developing novel therapeutic agents for various health issues.

10) Medicine:
Medicine refers to substances or practices used to promote health and treat illness. The research emphasizes the traditional medicinal uses of Moringa oleifera and Phyllanthus emblica, showcasing their bioactive compounds as essential components in the development of future medicinal formulations and therapies.

11) Phytochemical:
Phytochemicals are natural bioactive compounds found in plants that contribute to their therapeutic properties. The study identifies various phytochemicals extracted from Moringa oleifera and Phyllanthus emblica, establishing their potential roles in combating diseases, thus fostering interest in herbal remedies within modern medicine.

12) Tamilnadu (Tamil-nadu):
Tamil Nadu is a state in southern India known for its rich heritage and biodiversity. The study is conducted in this region, where traditional knowledge of medicinal plants, especially Moringa oleifera and Phyllanthus emblica, is prevalent, emphasizing their roles in local healthcare practices and wellness.

13) Tamil:
Tamil is a language predominantly spoken in the Indian state of Tamil Nadu and by Tamil communities worldwide. It represents a rich cultural and historical backdrop pertinent to the study, as traditional practices involving the researched medicinal plants are deeply rooted in Tamil lifestyle and medicinal history.

14) Nadu:
Nadu means 'land' or 'state' in Tamil, often used when referring to geographical regions within India. In this context, Tamil Nadu indicates the geographical area of study where Moringa oleifera and Phyllanthus emblica are harvested, emphasizing the significance of local flora in traditional medicine.

15) Knowledge:
Knowledge encompasses the understanding and insights gained through study, experience, or practice. In the research, traditional knowledge of medicinal plants plays a vital role in guiding the investigation of phytochemicals in Moringa oleifera and Phyllanthus emblica and their potential medical applications.

16) Madurai:
Madurai is a city in Tamil Nadu, India, known for its historical significance and cultural heritage. The research location emphasizes the relevance of local medicinal plants, such as Moringa oleifera and Phyllanthus emblica, which are traditionally utilized for various health benefits in this region.

17) Disease:
Disease refers to any abnormal condition affecting the body, often associated with specific symptoms and signs. The study focuses on medicinal plants that can provide therapeutic benefits for various diseases, advocating their use as natural remedies in traditional and modern medical practices.

18) Family:
Family, in a botanical context, refers to a classification level that groups related plants based on shared characteristics. Moringa oleifera and Phyllanthus emblica belong to different families (Moringaceae and Euphorbiaceae), underlining the diversity of plant compounds critical for medicinal chemistry.

19) Nature:
Nature encompasses the physical world including plants, animals, and ecosystems. The study examines the properties of naturally occurring phytochemicals in Moringa oleifera and Phyllanthus emblica, highlighting their role in health and wellness, rooted in biocultural connections to natural resources.

20) Tree:
Tree denotes a perennial plant with an elongated stem or trunk. Moringa oleifera, commonly known as the drumstick tree, and Phyllanthus emblica, the Amla tree, are traditional herbal medicine sources. Their plant parts are utilized for various therapeutic properties, emphasizing the importance of tree species in pharmacy.

21) Pharmacological:
Pharmacological pertains to the branch of medicine that deals with the study of drug action. The research investigates the pharmacological effects of identified phytochemicals from Moringa oleifera and Phyllanthus emblica, laying the groundwork for their development as therapeutic agents in natural medicine.

22) Tirunelveli:
Tirunelveli is a city in Tamil Nadu, India, representative of regions where Moringa oleifera and Phyllanthus emblica are cultivated. Its significance is essential in the context of natural resource utilization and the local knowledge systems surrounding the traditional use of these medicinal plants.

23) Science (Scientific):
Science is a systematic enterprise that builds and organizes knowledge through empirical evidence. The exploration of plant extracts like Moringa oleifera and Phyllanthus emblica within this study highlights the intersection of traditional knowledge and scientific inquiry, contributing to modern pharmacology.

24) Chennai:
Chennai is the capital city of Tamil Nadu, known for its educational and research institutions. The scientific aspects of the study, including spectroscopic analyses, contribute to the growing body of knowledge in pharmacological research centered around local medicinal plants significant in traditional medicine.

25) Species:
Species refers to a group of organisms capable of interbreeding. Moringa oleifera and Phyllanthus emblica are recognized species, often used in traditional medicine. Understanding their unique characteristics and biological significance is crucial for exploring their health benefits and therapeutic applications.

26) Medium:
Medium, in the context of science, relates to the materials or environment in which organisms grow or chemical processes occur. The petroleum ether serves as the extraction medium in the study to isolate bioactive compounds from the plants, enabling detailed analysis of their properties and benefits.

27) Powder:
Powder refers to a finely ground form of solid material. In the study, naturally dried and ground leaves of Moringa oleifera and Phyllanthus emblica are used to extract bioactive compounds, emphasizing the importance of the powder form in traditional remedies and phytochemical analysis.

28) Food:
Food represents any substance consumed to provide nutritional support to the body. The studied plants are not only utilized for medicinal purposes but also serve as nutritious food sources, highlighting their role in promoting health and wellness beyond traditional medicinal applications.

29) Emblica officinalis:
Emblica officinalis is the scientific name for Phyllanthus emblica, known for its rich vitamin C content and numerous health benefits. As a prominent plant in Ayurvedic medicine, its extracts are studied for pharmacological activities, emphasizing natural remedies and their therapeutic potentials.

30) Southern India:
Southern India is a geographical region encompassing several states, rich in biodiversity and traditional medicine practices. The specific climate and culture here support the growth of valuable medicinal plants like Moringa oleifera and Phyllanthus emblica, highlighting the region's contribution to herbal medicine.

31) Maharashtra (Maharastra, Maha-rashtra):
Maharashtra is a state in western India known for its diverse flora and traditional medicinal practices. The mention of Maharashtra in the study underscores the geographical diversity in the phytochemical profiles of Moringa oleifera, suggesting variations in compounds based on location and environmental factors.

32) Krishnaveni (Krsnaveni, Krishna-veni):
Krishnaveni appears to reference a researcher or an individual associated with studies on phytochemistry or traditional medicine. Their contributions signify the ongoing efforts in understanding the biological properties of medicinal plants in relation to health benefits and applications in healthcare.

33) Discussion:
Discussion refers to the portion of a scientific paper where data interpretations, implications, and contextual analysis occur. The discussion in this study reflects the significance of the findings obtained from Moringa oleifera and Phyllanthus emblica's bioactive compounds, emphasizing their therapeutic relevance.

34) Meenakshi:
Meenakshi likely refers to Meenakshi Mission Hospital, associated with healthcare and clinical research. Mentioning this institution reinforces the collaborative efforts between traditional medicinal research and modern medical practices in assessing the therapeutic potential of local plants for broader health applications.

35) Substance:
Substance denotes any material with specific properties, whether natural or synthetic. The study evaluates various natural substances based on their phytochemical constituents in Moringa oleifera and Phyllanthus emblica, crucial for understanding their potential medicinal uses and benefits in traditional healthcare systems.

36) Flavonoid:
Flavonoid refers to a class of plant metabolites known for their antioxidant and anti-inflammatory properties. While specific flavonoids were not highlighted in the study, their relevance in traditional plant medicine underpins the health benefits attributed to Phyllanthus emblica and Moringa oleifera.

37) Transformation (Transform, Transforming):
Transform indicates a change in form or structure, often related to chemical processes. In this context, the transformation of plant materials into extracts allows for the identification and study of bioactive compounds, revealing their potential health benefits and therapeutic roles in medicine.

38) Pesticide:
Pesticide refers to substances used to eliminate pests, often in agricultural settings. The implication of discussing pesticides in accordance with medicinal plants pertains to ensuring the safety and efficacy of plant-derived compounds, focusing on their natural holistic properties in alternative medicine.

39) Relative:
Relative denotes the relationship between two or more entities. In scientific research, relative comparisons of bioactive compound presence, efficacy, or concentration allow for a better understanding of plant properties, leading to improved assessments of medicinal effectiveness between different plant species.

40) Cancer:
Cancer denotes a group of diseases characterized by abnormal cell growth with the potential to invade or spread to other parts of the body. The study's findings emphasize the presence of bioactive compounds with possible anti-cancer properties, demonstrating the relevance of traditional plants in cancer treatment strategies.

41) Purity:
Purity refers to the extent to which a substance is free from contaminants or mixtures. In phytochemistry, assessing the purity of plant extracts is crucial for evaluating their medicinal efficacy, ensuring that the studied bioactive compounds from Moringa oleifera and Phyllanthus emblica remain effective.

42) Insect:
Insect denotes a class of organisms that can have both beneficial and detrimental effects on agriculture. The study indirectly references the role of medicinal plants in pest control and as natural insect repellents, enhancing the ecological importance of plants like Moringa oleifera and Phyllanthus emblica in sustainable practices.

43) Indian:
Indian refers to anything originating from or related to India, encompassing its rich cultural, historical, and medicinal traditions. The study prominently features Indian medicinal plants, emphasizing the importance of indigenous knowledge systems that guide the understanding and development of natural healthcare practices.

44) Botany:
Botany is the scientific study of plants, including their structure, properties, and biochemical processes. The study's focus on the phytochemical constituents of Moringa oleifera and Phyllanthus emblica highlights the contributions of botany to understanding medicinal plants and their applications in health and medicine.

45) Lanka (Lamka, Lànkà):
Lanka, commonly associated with Sri Lanka, refers to the geographical and cultural context of certain medicinal plants. The historical trade and exchange of plant knowledge between India and Lanka showcase the significance of these regions in preserving traditional medicinal practices and the use of phytochemicals.

46) Kumar:
Kumar likely refers to a researcher or author mentioned within the study whose work contributes to the understanding of phytochemicals and their health impacts. Their previous findings help build a comprehensive perspective on the medicinal benefits of compound analysis from various plant sources.

47) Water:
Water serves as a universal solvent and is essential for the extraction processes in phytochemistry. In the study, the use of water in extraction methods emphasizes its critical role in isolating bioactive compounds from plants like Moringa oleifera and Phyllanthus emblica for further analysis.

48) Kovil:
Kovil translates to 'temple' in Tamil, often indicating the cultural significance associated with nature and local flora. The collected plant samples were sourced from Kovil hills, showcasing the fusion of cultural reverence and natural resource utilization for traditional medicinal practices in Tamil Nadu.

49) Horse:
Horse relates to the common name of Moringa oleifera, known as the horse radish tree. This name reflects the plant’s traditional uses and attributes, underpinning its importance in local culture and herbal medicine, particularly in regions where it is cultivated and used.

50) Field:
Field pertains to the domain or context of knowledge or study. In this research, it refers to the field of herbal medicine and pharmacognosy, emphasizing the importance of understanding the medicinal properties of plants like Moringa oleifera and Phyllanthus emblica for therapeutic developments.

51) Fever:
Fever denotes an elevated body temperature, often indicating an underlying health issue or infection. The traditional application of Moringa oleifera and Phyllanthus emblica in treating fever showcases their historical roles in combating various ailments, expanding the understanding of their therapeutic benefits.

52) Cina:
China is noted for its extensive use of medicinal plants, which overlap with some indigenous practices in India. The mention of China contextualizes the broader Eastern medicinal landscape, highlighting the shared botanical knowledge and the potential global relevance of the phytochemicals derived from Moringa oleifera and Phyllanthus emblica.

53) Amla:
Amla is the common name for Phyllanthus emblica, renowned for its high vitamin C content and significant medicinal properties in Ayurvedic practice. Its presence in the study emphasizes the plant's importance in health and nutrition and its role in traditional formulations for wellness.

54) Jeya:
Jeya likely refers to Jeya College of Arts and Science, associated with academic research on medicinal plants. Its mention underscores the academic connections and institutional support vital for scientific exploration into the pharmacological benefits of Moringa oleifera and Phyllanthus emblica.

55) Bile:
Bile is a digestive fluid produced by the liver, playing a crucial role in fat digestion and absorption. Research may highlight the potential role of certain bioactive compounds from Moringa oleifera and Phyllanthus emblica in supporting liver health and contributing to efficient digestion and metabolic functions.

56) Genu:
Genu relates to authenticity and lineage, emphasizing the significance of understanding and preserving traditional knowledge in herbal medicine. The exploration of Moringa oleifera and Phyllanthus emblica in the study reflects the need to maintain genuine practices rooted in local tradition and cultural practices.

57) Rich (Rch):
Rich indicates the abundance of valuable resources, including medicinal plants. The study highlights the richness of phytochemical diversity found in Moringa oleifera and Phyllanthus emblica, underscoring their significance within the realms of traditional medicine and contemporary pharmacological research.

Other Science Concepts:

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Discover the significance of concepts within the article: ‘GC-MS analysis of bioactive compounds in Moringa and Emblica extracts.’. Further sources in the context of Science might help you critically compare this page with similair documents:

Phyllanthus emblica, Active principle, Traditional medicine, Phytochemical screening, Moringa oleifera, Clinical application, Bioactive compound, Biologically active phytoconstituents, Chemical constituent, Gas Chromatography Mass Spectrometry, Active fraction, Functional group.

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