Q-absorbance ratio method for cilostazol and imipramine estimation
Journal name: World Journal of Pharmaceutical Research
Original article title: Q-absorbance ratio method for simultaneous estimation of cilostazol and imipramine in combined dosage form
The WJPR includes peer-reviewed publications such as scientific research papers, reports, review articles, company news, thesis reports and case studies in areas of Biology, Pharmaceutical industries and Chemical technology while incorporating ancient fields of knowledge such combining Ayurveda with scientific data.
This page presents a generated summary with additional references; See source (below) for actual content.
Original source:
This page is merely a summary which is automatically generated hence you should visit the source to read the original article which includes the author, publication date, notes and references.
Paras M. Brahmbhatt, Laxman M. Prajapati, Amit K. Joshi, Mohammadali L. Kharodiya
World Journal of Pharmaceutical Research:
(An ISO 9001:2015 Certified International Journal)
Full text available for: Q-absorbance ratio method for simultaneous estimation of cilostazol and imipramine in combined dosage form
Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research
Copyright (license): WJPR: All rights reserved
Download the PDF file of the original publication
Summary of article contents:
Introduction
The manuscript presents a Q-absorbance ratio method developed for the simultaneous estimation of cilostazol and imipramine in combined dosage forms. This method is characterized as simple, sensitive, accurate, precise, and economical, facilitating the determination of both drugs. Cilostazol is a selective inhibitor of phosphodiesterase type 3, known for its therapeutic effects on blood flow and platelet aggregation. Imipramine, a tricyclic antidepressant, works by inhibiting neurotransmitter reuptake. Despite the common use of these drugs in clinical settings, a validated method for their combined estimation has not been officially documented.
Important Concept: Isoabsorptive Point
One significant aspect of the Q-absorbance ratio method is the identification of an isoabsorptive point. Cilostazol and imipramine exhibit an isoabsorptive point at 233.400 nm in methanol, which is crucial for the method's accuracy. The method requires absorbance measurements at both this isoabsorptive point and at the λ-max of one of the components—in this case, 258 nm, the λ-max for cilostazol. This approach leverages the unique properties of the drugs at specific wavelengths, allowing for reliable quantitative analysis in their combined formulations.
Method Validation
The method was rigorously validated following ICH guidelines, including assessments of linearity, precision, accuracy, and limits of detection (LOD) and quantification (LOQ). Both cilostazol and imipramine showed linear responses within the concentration range of 10-35 μg/ml. The precision of the method, measured through repeatability and intra- and inter-day variability, demonstrated low relative standard deviations (% RSD), confirming high reproducibility. The accuracy, evaluated via recovery studies, revealed recovery rates ranging from 98.03% to 99.54% for both drugs, signifying that the method is robust and reliable.
Application in Pharmaceutical Analysis
The applicability of the Q-absorbance ratio method was tested on a commercially available injection formulation. Analysis showed that the concentrations of cilostazol and imipramine in the marketed preparation were consistent with label claims, thus validating the method for routine analysis. The results highlighted that both drugs can be quantitatively determined without interference from excipients, ensuring that the method can be effectively utilized in pharmaceutical quality control.
Conclusion
In summary, the developed Q-absorbance ratio method provides a reliable, efficient, and cost-effective means for the simultaneous estimation of cilostazol and imipramine in combined dosage forms. The robustness of the method, underscored by validation results, presents a significant advancement for pharmaceutical analysis in this area. Its ease of use and high accuracy make it ideal for routine applications in drug formulation testing and quality assurance practices. The research received support from local pharmaceutical companies, highlighting the collaborative effort in advancing pharmaceutical methodologies.
FAQ section (important questions/answers):
What is the purpose of the Q-absorbance ratio method?
The Q-absorbance ratio method is used for the simultaneous estimation of cilostazol and imipramine in combined dosage forms, providing a sensitive and accurate analysis.
What are cilostazol and imipramine used for?
Cilostazol is used to inhibit platelet aggregation and improve blood flow, while imipramine is an antidepressant that increases serotonin and norepinephrine levels in the brain.
What solvents are used in the Q-absorbance ratio method?
Methanol is the chosen solvent for both cilostazol and imipramine as they are soluble in it, facilitating effective spectrophotometric analysis.
How were the calibration curves constructed in this study?
Calibration curves for cilostazol and imipramine were constructed by plotting absorbances against varying concentrations after measuring the absorbance at specific wavelengths.
What were the limits of detection and quantification for the drugs?
The limits of detection were 0.026 μg/ml for cilostazol and 0.069 μg/ml for imipramine, while the limits of quantification were 0.077 μg/ml and 0.209 μg/ml, respectively.
How accurate is the proposed spectrophotometric method?
The method demonstrated high accuracy, with recoveries for cilostazol ranging from 98.45% to 99.54% and for imipramine between 98.03% and 101.94%.
Glossary definitions and references:
Scientific and Ayurvedic Glossary list for “Q-absorbance ratio method for cilostazol and imipramine estimation”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.
1) Drug:
In the context of the article, 'Drug' refers to the active compounds being studied—cilostazol and imipramine. These substances are used for therapeutic purposes; cilostazol is primarily for vasodilation and preventing clotting, while imipramine is an antidepressant. Understanding their interactions and effective dosage forms is crucial for developing safe and effective medication.
2) Para (Para°, Párá):
'Para' is a prefix and also a common abbreviation used in research publications, particularly scientific contexts. It indicates a part or section of the paper. In this instance, 'Paras' represents one of the authors, signifying their contribution to the study and reinforcing collaboration within pharmaceutical research.
3) Table:
'Table' denotes structured data presentation that summarizes results and findings systematically. In this manuscript, tables are utilized to display the analysis of validation parameters, accuracy results, and various metrics critical for assessing the spectrophotometric methods employed. Tables enhance clarity, allowing readers to interpret results quickly and efficiently.
4) Gujarat:
'Gujarat' is a state in western India, where the study's institutional affiliations are located. The mention of Gujarat signifies the geographical context of the research institutions and may hint at regional collaborations or specificity in drug formulation practices, reflecting local pharmaceutical education standards and regulatory environments.
5) Education:
'Education' in this context pertains to pharmaceutical education and research development. This term suggests the collaborative aspect of the study, highlighting the importance of academic institutions in advancing pharmaceutical sciences, fostering research capabilities, and contributing to the training of professionals in the field of pharmacy.
6) India:
'India' is the country where the research took place and where the authors are affiliated. The mention of India emphasizes the nation's active role in pharmaceutical research and development. It also indicates the local significance and accessibility of the studied medications within the Indian healthcare system.
7) Shri (Sri, Sr):
The term 'Shri' is an honorific used in India to denote respect and is often prefixed to names, especially among professionals and academics. In this article, it precedes the names of authors and indicates recognition of their contributions and standing within the academic and professional communities.
8) Sah:
'Shah' is part of the name 'Shri B. M. Shah College of Pharmaceutical Education & Research,' indicating the affiliation of the authors to this specific institution. It suggests a legacy of contributions to pharmacological education and research, emphasizing the role of the institution in shaping healthcare professionals.
9) Measurement:
'Measurement' is crucial in the context of the study, referring to the quantification of drug concentrations through the Q-absorbance ratio method. Accurate measurement ensures the reliability of results. The term implies technical precision, reflecting methodologies that uphold scientific rigor in pharmaceutical analysis.
10) Discussion:
'Discussion' is a section of the paper where results are analyzed and interpreted in the context of existing literature. It provides insights into the relevance, applicability, and implications of findings. This part is significant for contextualizing the study within broader research themes and reinforcing the significance of the results.
11) Depression:
'Depression' is a clinical concern addressed by imipramine, a medication used to manage this mental health condition. Recognizing its relevance emphasizes the therapeutic importance of the drug and encourages ongoing research into effective treatments, combining psychopharmacology with patient outcomes in mental health management.
12) Relative:
'Relative' indicates relationships or comparisons made between different measurements or findings within the study. In the context of pharmacology, relative comparisons—such as those between drug concentrations and effects—are fundamental for determining dosage levels and understanding drug interactions, ensuring effective treatment protocols.
13) Campu:
'Campu' likely refers to the campus of the Shri B. M. Shah College of Pharmaceutical Education & Research. This term underscores the educational environment where research is conducted, highlighting the role of campus facilities in fostering collaboration, innovation, and training aspiring pharmacists and researchers.
14) Joshi (Josi):
'Joshi' is one of the authors of the study, underscoring individual contributions to the research. Including author names reflects collaborative efforts in pharmaceutical research, highlighting the importance of teamwork in advancing the understanding of drug interactions and methodologies in medicinal chemistry.
15) Blood:
'Blood' is indirectly related to cilostazol, primarily known for its effects on blood circulation and platelet aggregation. The mention underscores the pharmacological importance of cilostazol in managing vascular health and potentially correlates with its clinical applications in treating conditions related to blood flow and clots.
16) Study (Studying):
'Study' refers to the detailed research investigation designed to evaluate the efficacy of the Q-absorbance ratio method for drug estimation. This term highlights the systematic approach used in the research, reflecting the meticulous planning necessary to ensure both validity and application of results in the pharmaceutical field.
17) Noise:
'Noise' refers to the background signals or fluctuations that can interfere with measurements in spectrophotometric analysis. Understanding and mitigating noise is crucial in achieving accurate, reproducible results and emphasizes the importance of rigorous methodology to enhance the reliability of the obtained data.
18) Line:
'Line' in this research likely pertains to linearity in the calibration curves express correlations between drug concentration and absorbance. Establishing a linear relationship is fundamental for validating analytical methods, ensuring that results can be appropriately interpreted and reliably applied in clinical settings.
Other Science Concepts:
Discover the significance of concepts within the article: ‘Q-absorbance ratio method for cilostazol and imipramine estimation’. Further sources in the context of Science might help you critically compare this page with similair documents:
Central nervous system, Literature survey, Recovery study, Regression analysis, Therapeutic focus, Limit of detection, Limit of quantification, Sample preparation, Calibration curve, Limit of Detection (LOD), Limit of Quantification (LOQ), Pharmaceutical formulation, UV-visible spectrophotometer, Method validation, Spectrophotometric determination, Relative standard deviation, Spectrophotometric analysis, Routine Analysis, Spectrophotometric estimation, Standard solution, Molecular structure, Tricyclic antidepressant, Digital analytical balance.