Alizarin dye preconcentration using β-CD epichlorohydrin polymer.

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Journal name: World Journal of Pharmaceutical Research
Original article title: Preconcentration of alizarin dye using betacyclodextrin epichlorohydrin polymer as a solid phase extractant.
The WJPR includes peer-reviewed publications such as scientific research papers, reports, review articles, company news, thesis reports and case studies in areas of Biology, Pharmaceutical industries and Chemical technology while incorporating ancient fields of knowledge such combining Ayurveda with scientific data.
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Original source:

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Author:

Amandeep Kaur and Dr. Usha Gupta.


World Journal of Pharmaceutical Research:

(An ISO 9001:2015 Certified International Journal)

Full text available for: Preconcentration of alizarin dye using betacyclodextrin epichlorohydrin polymer as a solid phase extractant.

Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research

Copyright (license): WJPR: All rights reserved


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Summary of article contents:

Introduction

This study focuses on the preconcentration of alizarin dye using a newly synthesized β-Cyclodextrin epichlorohydrin polymer (β-CDP) as a solid-phase extraction material. Alizarin, a red dye originally derived from the madder plant, has significant applications in textiles, clinical staining, and biomedical research. However, alizarin dye is hazardous, posing risks of toxicity and carcinogenic effects, necessitating stringent control over its concentration in various samples. The method presented here utilizes UV-Vis spectrophotometry, known for its sensitivity and cost-effectiveness, to detect and quantify alizarin dye in different matrices following preconcentration.

Optimization of Preconcentration Conditions

The study explores several conditions to optimize the preconcentration of alizarin dye using β-CDP. Key parameters include pH, sample volume, amount of polymer, and shaking time. The investigation revealed that an optimal pH of 4.0 yielded the highest recovery of alizarin, while sample volumes up to 35.0 ml were suitable for effective dye extraction. The amount of β-CDP polymer was determined to be ideal at 250 mg, ensuring maximum recovery, and a shaking time of 120 minutes was found to be effective for complete extraction of the dye. These findings establish critical conditions for maximizing the efficiency of the extraction process.

Synthesis of β-Cyclodextrin Polymer

The synthesis of the β-Cyclodextrin epichlorohydrin polymer was achieved by crosslinking β-cyclodextrins with epichlorohydrin under basic conditions. This process involved dissolving β-cyclodextrins and soluble starch in sodium hydroxide, followed by the gradual addition of epichlorohydrin, leading to the formation of a water-insoluble polymer. Post-synthesis, the polymer was characterized by its stability and ability to form stable complexes with hydrophobic molecules like alizarin. Such polymers serve as effective sorbents, enhancing the removal and preconcentration of dyes and other organic pollutants from aqueous solutions.

Applications of the Developed Method

The proposed extraction method was tested for its applicability in real-world scenarios by determining the concentration of alizarin dye in various spiked samples, including ink and commercial formulations. The results showcased recovery rates of 96.0% and 99.0% for the alizarin ink and alizarin crimson samples, respectively, demonstrating the method's reliability and efficiency in quantifying the dye. Table data reflected the effectiveness of the β-CDP in achieving high recoveries, indicating its potential for widespread use in environmental monitoring and quality control in dye manufacturing.

Conclusion

In conclusion, the synthesized β-Cyclodextrin epichlorohydrin polymer proves to be a promising and efficient solid-phase extractant for preconcentrating alizarin dye from various samples. The established method delivers quantitative recoveries while being simple, cost-effective, and repeatable. Furthermore, the polymer's ability to be regenerated enhances its practicality for repeated applications. Overall, this study presents a robust approach for the determination of alizarin dye, advancing techniques in both analytical chemistry and environmental safety.

FAQ section (important questions/answers):

What is the main purpose of this research article?

The research article discusses a method for preconcentrating alizarin dye using β-Cyclodextrin epichlorohydrin polymer as a solid phase extractant, utilizing UV-Vis spectrophotometry for dye determination.

What are the key benefits of using β-CDP in this method?

β-CDP provides a low-cost, simple, and efficient means for extracting alizarin dye from various samples, achieving a recovery rate of over 95%.

Why is controlling alizarin dye concentration important?

Alizarin dye poses health risks, including toxicity and potential carcinogenic effects, making it crucial to monitor and control its concentration in various applications.

What parameters were optimized in the study?

The study optimized parameters such as pH, sample volume, amount of β-CDP, and shaking time to enhance the recovery of alizarin dye.

How was β-CDP polymer synthesized in the experiment?

The β-CDP polymer was synthesized by cross-linking β-Cyclodextrin with epichlorohydrin in a sodium hydroxide solution and then drying the resulting polymer.

What was the recovery percentage of alizarin dye from samples?

The recovery percentage of alizarin dye from tested samples was found to be 96.0% to 99.0%, demonstrating the method's effectiveness in extraction.

Glossary definitions and references:

Scientific and Ayurvedic Glossary list for “Alizarin dye preconcentration using β-CD epichlorohydrin polymer.”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.

1) Water:
Water plays a crucial role in various chemical processes, including the synthesis of β-Cyclodextrin polymer and as a solvent for dissolving chemicals like alizarin dye. In laboratory settings, double distilled water ensures purity and avoids contamination, which is vital for accurate experimental results and reliable measurements in chemistry and research.

2) Beta:
Beta (β) refers specifically to β-Cyclodextrins, a class of molecules used as host compounds in supramolecular chemistry. Their unique structure allows them to form complexes with various substances, enhancing their utility in applications such as dye extraction and environmental remediation through solid phase extraction techniques.

3) Measurement:
Measurement in the context of this research refers to the quantification of the alizarin dye concentration using a UV-vis spectrophotometer. This process is integral to determining the effectiveness of the extraction method and ensuring that results are accurate and reproducible, which underpins the scientific reliability of the study.

4) India:
India is the geographical context in which the research was conducted, specifically at Punjabi University Patiala. The study highlights local educational efforts in chemistry and environmental science, addressing issues relevant to India’s textile industry and chemical safety regulations, where dye pollution is a significant concern.

5) Table:
A table presents clear and organized data for the results of the study, showing the amounts of alizarin dye added versus found in various samples. Tables are essential in scientific publications for summarizing experimental results, facilitating quick comprehension, and allowing for straightforward comparisons between different experimental conditions.

6) Human body:
The human body serves as a historical substrate for dyes, as mentioned in the context of alizarin. Dyes affect human health, with alizarin being used in medical applications for staining tissues. Understanding interactions between dyes and biological systems is crucial for evaluating health risks related to dye exposure.

7) Inflammation:
Inflammation is a concern when discussing the toxic effects of alizarin dye on human health, particularly through skin exposure. Chronic inflammation can affect cellular health and lead to wider health issues. The study emphasizes the need for methods to control and monitor dye concentrations to mitigate such toxicity.

8) Substance:
In this research, a substance refers to any material being studied or extracted, particularly the alizarin dye and the β-Cyclodextrin polymer. Understanding how these substances interact during the extraction process is central to the research, determining their effectiveness and potential applications in various fields, including environmental science.

9) Activity:
Activity denotes the chemical reactivity of substances, such as the interaction between alizarin dye and β-CD polymer. The study examines how varying conditions like pH and shaking time influence this activity, thereby affecting the efficiency of dye extraction, which is a critical aspect of the overall methodology.

10) Colouring (Coloring):
Coloring describes the use of dyes like alizarin, which provides coloration to materials such as textiles and biological substances. Alizarin’s historical significance as a dye reflects its application across various fields, indicating the intertwining of chemistry, art, industry, and health-related assessment in contemporary research.

11) Gujarat:
Gujarat is mentioned as the region where Bhatia Color Company operates, providing commercial context for the study. The presence of local companies highlights the relevance of the research to regional industry practices in dye usage and safety, emphasizing the need for effective detection and control methods for hazardous substances.

12) Chennai:
Chennai is mentioned as the location of Fischer Chemic Ltd, which produces alizarin ink. This underscores how research and industry are interconnected in India, demonstrating regional contributions to chemical manufacturing and the importance of developing analytical methods to ensure product safety and environmental compliance.

13) Painter:
Painters historically utilized alizarin dye to create rich colors in artworks. In the context of this study, the dye's properties and safety are investigated for modern applications, indicating the ongoing relevance of traditional materials in contemporary settings, especially regarding safety assessments for artists and the public.

14) Company:
The term company refers to the commercial entities involved in producing or utilizing alizarin dye, such as Fischer Chemic Ltd and Bhatia Color Company. These companies represent the intersection of commerce and science, highlighting the practical implications of research findings for manufacturing and regulatory practices in dye production.

15) Fabric:
Fabric serves as a primary substrate for dyes like alizarin, which have been historically significant for coloring textiles. The link between fabric and dye extends to contemporary concerns about dye safety, environmental impact, and the need for cleaner technologies in the dyeing process is addressed in the study.

16) Gupta:
Dr. Usha Gupta is one of the authors of the research, indicating her role in the academic and scientific endeavor. The inclusion of author names in studies is vital for attributing credit and fostering collaborations that advance research in fields such as chemistry and environmental science.

17) Glass:
Glass is referenced in the context of using quartz cells in UV-vis spectrophotometry measurements. The use of glass instruments is standard in laboratory settings as they provide stability and transparency necessary for accurate optical measurements, which are critical for analyzing the concentration of substances like alizarin dye.

18) Blood:
Blood is mentioned in relation to the harmful effects of alizarin when it enters the bloodstream, highlighting the importance of understanding the biological implications of chemical exposure. This connects the study to broader issues of toxicology and health safety, underlining the need for analytical methods to monitor hazardous substances.

19) Usa (Usha):
Usha refers to Dr. Usha Gupta, co-author of the study. Recognizing contributors emphasizes collaborative efforts in research, which is essential for advancing knowledge in scientific fields. Such collaborations often lead to innovative methodologies and comprehensive findings, enriching the understanding of complex processes like dye extraction and toxicity.

20) Genu:
Genu refers to the genus of plants from which alizarin is derived, specifically the madder genus. Understanding the botanical origins of dyes contributes valuable context to their historical uses and chemical properties, reflecting the relationship between nature and synthetic processes in dye manufacturing and applications in various fields.

Other Science Concepts:

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Discover the significance of concepts within the article: ‘Alizarin dye preconcentration using β-CD epichlorohydrin polymer.’. Further sources in the context of Science might help you critically compare this page with similair documents:

Toxicity, Analytical method, Clinical Practice, Quantitative analysis, Solid-phase extraction, Recovery percentage, Long-term exposure, Spectrophotometric determination, Spectrophotometry, Double distilled water, Hazardous effect, Carcinogenic effect, Sample volume, Organic pollutant, Food chem.

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