Development and validation of method for determination of lutein by hplc

Glossary definitions and references:

Scientific and Ayurvedic Glossary list for “Development and validation of method for determination of lutein by hplc”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.

1) Table:
Table refers to a systematic arrangement of data in rows and columns, facilitating easy reference and comparison. In scientific research, tables present essential findings, such as assay results and method validations, in an organized fashion, allowing for straightforward interpretation and communication of complex information.

2) Water:
Water serves as a critical solvent in numerous analytical methods, particularly in chromatography. It aids in the preparation of mobile phases and diluents, ensuring the desired composition for effective separation of analytes. Its purity (HPLC grade) is vital for achieving reproducible and accurate results in experiments.

3) Relative:
Relative quantitative measures express the ratio or comparison of one quantity to another, essential in determining the consistency of results within analytical methods. In the context of chromatography, relative measurements like percent recovery and relative standard deviation are crucial for assessing method reliability and accuracy.

4) Study (Studying):
A study is an investigative process aimed at understanding a particular phenomenon, usually involving systematic methodology. In pharmaceutical research, studies are conducted to explore drug formulations, validate analytical methods, and analyze interactions of compounds, contributing to advancements in pharmaceutical sciences and therapeutic developments.

5) Performance:
Performance in analytical chemistry refers to the efficiency and effectiveness of an analytical method in measuring the target analyte. Key performance indicators in the context of HPLC analysis include accuracy, precision, sensitivity, and specificity, all critical for evaluating how well a method meets predefined scientific standards.

6) Measurement:
Measurement is the process of quantifying a property's value or quantity using specific techniques or instruments. In the realm of HPLC, measurement involves assessing concentration levels of compounds in a sample, ensuring that the results align with regulatory standards and facilitate quality control in pharmaceuticals.

7) Quality:
Quality refers to the degree of excellence of a product or process, typically involving stringent criteria and standards. In pharmaceutical research, quality of drugs and analytical methods is paramount to ensure safety, efficacy, and compliance with regulatory frameworks, underscoring the importance of rigorous testing and validation.

8) Food:
Food represents a significant source of various bioactive compounds, including carotenoids like lutein. Understanding the composition and concentration of these compounds in food products through analytical methods is essential for nutritional research, product formulation, and meeting health claims, thereby bridging food science and health.

9) Reliability:
Reliability in analytical methods emphasizes the consistency and dependability of results across multiple trials or conditions. This aspect is vital for gaining confidence in the data produced, especially in quality control and regulatory submissions, where reproducible findings ensure the credibility of scientific conclusions.

10) Discussion:
Discussion is a critical component of scientific reporting that interprets data, evaluates findings, and relates them to existing knowledge. It allows researchers to contextualize results, propose implications, and identify areas for future study, fostering advancement and innovation within the field of research.

11) Substance:
Substance generally refers to a material with a defined composition, often being the subject of analysis in scientific research. In the pharmaceutical context, substances can denote active pharmaceutical ingredients or excipients, where precise identification and quantification are crucial for ensuring product efficacy and safety.

12) Observing:
Observing is the act of carefully examining and recording phenomena or results in research. In a laboratory setting, observing chromatographic separations and their characteristics helps scientists understand and enhance analytical methods, leading to improved results and valuable insights into chemical behaviors.

13) Isha (Isa):
Isha refers to a specific individual involved in the study, Md. Isha Tareque, contributing to the research team's efforts. The inclusion of team members highlights the collaborative nature of scientific research, showing how diverse expertise can enhance the development and validation of methods in pharmaceutical analysis.

14) Milk:
Milk is a complex biological fluid rich in nutrients, posing challenges in the analysis of components like lutein due to its fat content. Analytical methods tailored for dairy products often use specific techniques to ensure accurate quantification of bioactive compounds, impacting nutrition and dietary formulations.

15) Drug:
A drug is a substance used for diagnosis, treatment, or prevention of disease. The development and validation of analytical methods for drug components, like lutein, are essential for ensuring that pharmaceuticals meet regulatory standards and provide therapeutic benefits, influencing public health outcomes.