Hplc-ms, hrms analysis of microbial acid free, short chain alkyl sophorosides

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Journal name: World Journal of Pharmaceutical Research
Original article title: Hplc-ms, hrms analysis of microbial acid free, short chain alkyl sophorosides
The WJPR includes peer-reviewed publications such as scientific research papers, reports, review articles, company news, thesis reports and case studies in areas of Biology, Pharmaceutical industries and Chemical technology while incorporating ancient fields of knowledge such combining Ayurveda with scientific data.
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Author:

Vrushali Dengle-Pulate, Parul Dubey, Sunil Bhagwat and Asmita Prabhune


World Journal of Pharmaceutical Research:

(An ISO 9001:2015 Certified International Journal)

Full text available for: Hplc-ms, hrms analysis of microbial acid free, short chain alkyl sophorosides

Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research

Copyright (license): WJPR: All rights reserved


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Summary of article contents:

Introduction

Sophorolipids (SL) are a class of glycolipid biosurfactants produced by specific yeast strains, notably Candida bombicola, using various feedstocks. These compounds exhibit excellent surfactant properties due to their amphipathic structures and have gained attention in recent years for their biodegradability and low ecotoxicity, positioning them as environmentally friendly alternatives to synthetic surfactants. This research focuses on the synthesis of acid-free and short-chain sophorolipids using lauryl alcohol as a lipophilic carbon source, exploring their structural characterization and potential applications through advanced analytical techniques.

Creative Synthesis of Sophorolipids

The production of sophorolipids in this study was achieved by employing lauryl alcohol as a hydrophobic source. The researchers developed a method that allows for the incorporation of glycosidic linkages between sophorose and lauryl alcohol without having to block the yeast’s β-oxidation pathway, thus facilitating the synthesis of new-to-nature alkyl sophorosides. Various forms of sophorolipids were generated, including monoacetylated and diacetylated variants, through modifications that resulted in structures with enhanced polar features. These modifications are crucial for broadening the application spectrum of sophorolipids by improving their surface activity and solubility.

Zeta Potential and Stability of SLLA

The stability of the synthesized lauryl alcohol-derived sophorolipid (SLLA) was assessed through zeta potential measurements across different pH values. The study revealed that SLLA exhibited moderate stability, particularly at pH 5.0, with a zeta potential of -20.45. It was determined that the lack of charged polar groups limited surface charge and, hence, contributed to lower repulsion within the solution. Understanding the zeta potential is significant for its applications in formulations such as germicides, shampoos, and lotions, where stability against aggregation is essential.

Advanced Characterization Techniques

The researchers combined various analytical techniques, including MALDI-TOF/TOF and HPLC-MS, to thoroughly characterize the structures of the synthesized sophorolipids. The findings indicated that the major ions correspond to different alkyl sophorosides with specific acetylation at various positions, displaying varying degrees of surface activity. The high-resolution mass spectrometry (HR-MS) provided precise measurements of molecular weights, allowing for the identification of the novel structures, which were confirmed to possess distinctive end groups, such as hydroxyl and acetylated forms, enhancing their physicochemical properties.

Conclusion

This research successfully demonstrated the potential of lauryl alcohol as a feedstock for sophorolipid production by C. bombicola. The innovative methods used in this study not only provided acid-free and short-chain sophorolipids but also highlighted the importance of structural modifications in enhancing the properties of these biosurfactants. The findings underline the versatility of sophorolipids for various industrial applications and exhibit the efficacy of advanced characterization techniques in revealing the intricate structures of these compounds. Future research could explore further applications of these bioproducts in diverse fields, emphasizing sustainable practices in surfactant production.

FAQ section (important questions/answers):

What are sophorolipids and why are they important?

Sophorolipids are glycolipid biosurfactants produced by certain yeasts. They are biodegradable, non-toxic, and have good surface-active properties, making them valuable for industrial applications such as surfactants and emulsifiers.

How were the sophorolipids synthesized in this study?

The study synthesized sophorolipids using lauryl alcohol as a hydrophobic source and glucose as a hydrophilic source, employing the yeast Candida bombicola to produce acid-free and short-chain sophorose lipids.

What techniques were used to analyze sophorolipids?

Various techniques were employed, including HPLC-MS, HRMS, and MALDI-TOF/TOF for identifying the molecular structures and assessing the surface-active properties of the synthesized sophorolipids.

What is the significance of zeta potential in this research?

Zeta potential indicates the stability of colloidal dispersions. In this study, the zeta potential of sophorolipid solution was measured to assess its stability for potential applications in germicidal formulations and personal care products.

What were the findings related to the structure of synthesized sophorolipids?

The research discovered novel sophorolipids with variations in acetylation and terminal groups, affecting their physicochemical properties, including improved surface activity and potential for enhanced antimicrobial effectiveness.

How does the use of lauryl alcohol benefit sophorolipid production?

Lauryl alcohol, being a medium-chain primary alcohol, is an economical and efficient substrate for producing sophorolipids. It allows for significant incorporation into the biosynthetic pathway without blocking metabolic processes.

Glossary definitions and references:

Scientific and Ayurvedic Glossary list for “Hplc-ms, hrms analysis of microbial acid free, short chain alkyl sophorosides”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.

1) Surface:
The term 'surface' refers to the outermost layer of a material, where interactions and chemical reactions occur, especially relevant in biosurfactants like sophorolipids. The surface activity of these compounds affects their ability to lower surface tension and interact with other substances, vital for applications in detergents and emulsifiers.

2) Medium:
'Medium' indicates the nutrient solution in which microorganisms, such as Candida bombicola, grow and produce sophorolipids. The choice of medium composition, including glucose and lauryl alcohol, directly influences the yield and nature of the produced biosurfactants, making it a significant factor in microbial fermentation processes.

3) India:
'India' highlights the geographical context of the study, where the research was conducted at institutions like the Institute of Chemical Technology and the National Chemical Laboratory. The mention of India also underscores the region's significance in scientific research on sustainable biosurfactant production and renewable resources.

4) Water:
'Water' is an essential solvent in biochemical processes, including the solubilization of compounds and as a medium for microbial fermentation. In the context of the study, water serves as a key component in preparing the culture medium, influencing the solubility and reactivity of other components in producing sophorolipids.

5) Nature:
'Nature' refers to the natural occurrence and sources of sophorolipids that were initially discovered in specific yeast species. The study explores the synthetic production of 'new-to-nature' sophorolipids, emphasizing the innovation in creating biosurfactants that retain beneficial properties while being engineered for particular applications.

6) Measurement:
'Measurement' denotes the quantification and analysis of physical properties, such as zeta potential, using various scientific techniques. Accurate measurements in the study ensure that the properties of produced sophorolipids are understood, particularly their stability and effectiveness as surfactants, which impact their industrial applicability.

7) Science (Scientific):
'Science' encompasses the systematic enterprise that builds and organizes knowledge in the form of testable explanations and predictions about the universe. In this research, scientific methods and principles guide the exploration of biosurfactants, contributing to advancements in biochemical and environmental science for sustainable applications.

8) Activity:
'Activity' in the context of the research refers to the functional performance of sophorolipids as surfactants and their antimicrobial properties. The study examines how modifications in their chemical structure influence their activity, impacting applications in cleaning, personal care, and industrial processes, showcasing their potential utility.

9) Species:
'Species' relates to the specific organisms utilized in the production process, particularly the yeast Candida bombicola. Understanding the species involved is crucial for optimizing fermentation conditions and enhancing the production of sophorolipids, which are derived from natural biological processes and can exhibit unique properties.

10) Table:
'Table' is a reference to organized data presented within the research. In scientific literature, tables summarize findings succinctly, such as showing quantitative results related to the mass, structure, and composition of different sophorolipid variants produced, aiding in the interpretation and validation of research outcomes.

11) Study (Studying):
'Study' encompasses the systematic investigation conducted to explore the synthesis and properties of sophorolipids. A well-defined study design underpins the validity of the results and conclusions drawn regarding their production processes, characterization, and potential applications in diverse fields, including pharmaceuticals and biotechnology.

12) Pune:
'Pune' refers to the city in India where the National Chemical Laboratory is located. This highlights the local context of the research facilities and collaborative nature of scientific endeavors in the area, emphasizing Pune's role as a hub for biochemical and chemical research in the country.

13) Biodegradable:
'Biodegradable' is a key attribute of sophorolipids, signifying their capability to decompose naturally in the environment. This characteristic enhances their appeal as eco-friendly alternatives in industrial applications, reflecting growing environmental concerns and the demand for sustainable solutions in the production and use of surfactants.

14) Discussion:
'Discussion' typically refers to the section of scientific articles where the authors interpret findings, compare results, reflect on their significance, and propose future research directions. In this study, the discussion would encompass the implications of the results for practical applications and broader scientific understanding of biosurfactants.

15) Relative:
'Relative' denotes a comparative aspect often utilized in scientific research to assess properties or behaviors in context. In the study, relative assessments are crucial for evaluating the effectiveness and characteristics of new-to-nature sophorolipids against existing models or products, determining their competitive advantage.

16) Fainting (Fainted):
'Faint' describes the level of visibility or discernability of a substance, such as the faint brown color of the sophorolipid product noted in the methodology. This observation can provide insights into the purity and concentration of the treated material, relevant for subsequent analyses and characterizations.

Other Science Concepts:

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Discover the significance of concepts within the article: ‘Hplc-ms, hrms analysis of microbial acid free, short chain alkyl sophorosides’. Further sources in the context of Science might help you critically compare this page with similair documents:

Financial support, Antimicrobial agent, Chromatographic separation, Zeta potential, Antimicrobial studies, Experimental work, Flow rate, High yield, Biodegradability, Physico-chemical characterization, Technical support, Gas analysis.

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