In vitro antioxidant efficacy of turbinaria conoides

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Journal name: World Journal of Pharmaceutical Research
Original article title: In vitro antioxidant efficacy of turbinaria conoides
The WJPR includes peer-reviewed publications such as scientific research papers, reports, review articles, company news, thesis reports and case studies in areas of Biology, Pharmaceutical industries and Chemical technology while incorporating ancient fields of knowledge such combining Ayurveda with scientific data.
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Original source:

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Author:

J.Jayabarath and K.Jeyaprakesh


World Journal of Pharmaceutical Research:

(An ISO 9001:2015 Certified International Journal)

Full text available for: In vitro antioxidant efficacy of turbinaria conoides

Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research

Copyright (license): WJPR: All rights reserved


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Summary of article contents:

Introduction

The study explores the in vitro antioxidant efficacy of the marine seaweed Turbinaria conoides, which is widely recognized for its medicinal properties attributed to its rich phytochemical content. Conducted by J. Jayabarath and K. Jeyaprakesh, the research underscores the significance of phytochemicals found in seaweeds as potential therapeutic agents against various diseases. The investigation involved extracting phytochemicals using different solvents, primarily methanol, and evaluating their antioxidant activities using several established assays.

Presence of Phytochemicals

One of the significant findings was the presence of various phytochemicals in Turbinaria conoides. The study identified key compounds, including alkaloids, flavonoids, carbohydrates, phenolic compounds, proteins, and amino acids, through preliminary screening using aqueous, ethanol, and methanol extracts. Methanol proved to be the most effective solvent for extraction. This rich phytochemical profile highlights the potential of T. conoides as a valuable resource for antioxidant compounds that provide health benefits by combatting oxidative stress-related diseases.

Antioxidant Activity Assays

The research employed multiple methods to assess the antioxidant activity of Turbinaria conoides. These methods included the DPPH radical-scavenging assay, total antioxidant capacity, superoxide anion scavenging assay, iron chelating activity assays, and reducing power assays. Results indicated that the methanolic extract of T. conoides demonstrated substantial antioxidant activity across all tests, particularly exhibiting a significant scavenging effect on DPPH radicals and superoxide anions. This suggests that the extracts can effectively neutralize harmful free radicals, supporting their potential use in health applications.

Health Implications of Antioxidants

The study discusses the broader implications of dietary antioxidants, especially those derived from natural sources like seaweeds. It emphasizes that natural antioxidants, such as the polyphenols identified in T. conoides, can play a crucial role in preventing oxidative stress, which is associated with various chronic diseases like cancer, cardiovascular diseases, and neurodegenerative disorders. The research indicates that including seaweeds in diets can provide not only nutritional benefits but also protective qualities against oxidative damage.

Conclusion

In conclusion, the methanolic extracts of Turbinaria conoides exhibit remarkable in vitro antioxidant activity, positioning them as promising candidates for natural antioxidants in food supplements and pharmaceutical applications. The findings underline the significance of exploring marine resources for bioactive compounds that contribute to health and well-being. This research advocates for further investigations into the therapeutic uses of seaweeds and their potential role in promoting health through their antioxidative properties.

FAQ section (important questions/answers):

What are the key phytochemicals found in Turbinaria conoides?

The study identified various phytochemicals in Turbinaria conoides, including alkaloids, flavonoids, carbohydrates, phenolic compounds, proteins, and amino acids, particularly highlighting the presence of these compounds in methanolic extracts.

How was the antioxidant activity of Turbinaria conoides measured?

Antioxidant activity was measured using methods like Total Antioxidant Activity, DPPH scavenging assay, superoxide anion scavenging activity, ion chelating activity, and reducing power assay.

What solvents were used for extracting phytochemicals from Turbinaria conoides?

Extraction of phytochemicals was carried out using different solvents including water, ethanol, and methanol, with methanol showing the best results for antioxidant activity.

What was the effect of Turbinaria conoides on DPPH radicals?

Turbinaria conoides exhibited significant DPPH radical scavenging activity, achieving up to 99.34% inhibition at the highest concentration of 80 µg/ml.

Why are antioxidants important in human health?

Antioxidants help neutralize free radicals that can cause cellular damage and contribute to chronic diseases, thereby playing a crucial role in protecting human health.

What conclusions were drawn about Turbinaria conoides as a natural antioxidant?

The study concluded that the methanolic extracts of Turbinaria conoides demonstrate significant antioxidant properties, suggesting its potential use in food supplements and pharmaceuticals.

Glossary definitions and references:

Scientific and Ayurvedic Glossary list for “In vitro antioxidant efficacy of turbinaria conoides”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.

1) Activity:
In the context of the study, 'Activity' refers to the effectiveness of the extracts of *Turbinaria conoides* in exhibiting antioxidant properties. The assays conducted aim to quantify the scavenging capabilities against free radicals, providing insight into how active these compounds are in binding and neutralizing harmful species that cause oxidative stress in biological systems.

2) Phytochemical:
'Phytochemical' refers to the bioactive compounds derived from plants that have potential health benefits. In this study, phytochemicals extracted from *Turbinaria conoides* are analyzed for their antioxidant properties. Emphasis on phytochemicals highlights their role in combating diseases and oxidative damage, contributing to health and nutrition.

3) Table:
'Table' in this document serves as a structured format presenting data clearly, summarizing results from various experiments. Each table provides comparative insights into the antioxidant capacity and phytochemical composition of *Turbinaria conoides* extracts. This makes it easier for readers to digest and interpret the findings quantitatively.

4) Disease:
The term 'Disease' is significant as the study highlights the potential therapeutic applications of *Turbinaria conoides* in fighting oxidative stress-related ailments. The discussion addresses how antioxidants may mitigate the risks of chronic diseases such as cancer, cardiovascular conditions, and other health issues linked to free radicals.

5) Study (Studying):
'Study' is vital to the text as it encompasses the entire research effort to evaluate the antioxidant properties of *Turbinaria conoides*. This word signifies a systematic inquiry involving experimental design, data collection, and analysis to provide scientific evidence regarding the efficacy of seaweed extracts in biomedical contexts.

6) Water:
'Water' is one of the solvents used for extracting phytochemicals from *Turbinaria conoides*. Its mention underlines the importance of this universal solvent in the extraction process, facilitating an understanding of how water-soluble compounds contribute to the antioxidant capacity of the algae being studied.

7) Thanjavur:
'Thanjavur' is the geographical location of the research institution involved in the study. It indicates the place where the sampling and experiments were conducted, providing context and geographical relevance to *Turbinaria conoides*, which is sourced from the Gulf of Mannar in South East India.

8) Species:
'Species' denotes the specific reference to *Turbinaria conoides*, a type of brown algae studied for its antioxidant properties. Understanding species is integral in biological research as it allows scientists to categorize organisms and understand their unique biochemical properties and potential applications in health and disease management.

9) Food:
'Food' relates to the implications of the study findings in dietary contexts. The antioxidant properties of *Turbinaria conoides* suggest potential uses in food supplements, with natural antioxidants being preferred over synthetic alternatives. The study reflects a growing interest in health-conscious dietary choices supporting overall wellness.

10) Substance:
'Substance' refers to the active compounds extracted from *Turbinaria conoides*, emphasizing that these phytochemicals possess specific effects, such as antioxidant activity. This term indicates the significance of identifying and characterizing these materials, which can lead to applications in medicine, nutrition, and food industries.

11) Flavonoid:
'Flavonoid' is a class of compounds identified in the study, tied to antioxidant activities. These polyphenolic compounds are relevant for their multiple health benefits, including reducing inflammation and oxidative stress. The presence of flavonoids in *Turbinaria conoides* indicates its potential value in preventive healthcare and nutrition.

12) Powder:
'Powder' refers to the processed form of the *Turbinaria conoides* used in the extraction. By grinding the seaweed into powder, researchers maximize the surface area for solvent interaction, thus enhancing the efficiency of phytochemical extraction, which is crucial for analyzing antioxidant properties.

13) Coral:
'Coral' pertains to the marine ecosystem where *Turbinaria conoides* is found, as it primarily grows attached to coral substrates. This connection emphasizes the importance of biodiversity and ecological health in marine environments, providing a rich source of bioactive compounds with potential industrial applications.

14) Cina:
'China' appears in reference to the laboratory equipment used (China dishes), which are standard glassware in biological research for preparing and storing samples. This highlights the importance of proper laboratory materials in ensuring accurate and reliable scientific experimentation for studying antioxidant properties.

15) Dish (Dis):
'Dish' specifically refers to the shallow glass containers used for culturing and storing extracts in laboratory settings. Mentioning 'dish' underscores the significance of proper containment during the experimental process, which affects the quality and integrity of the extracted phytochemical samples.

16) Antibiotic (Antibacterial):
'Antibacterial' relates to the potential applications of *Turbinaria conoides* beyond antioxidant activity. The study hints at its efficacy against microbial infections, suggesting further examination of its antibacterial properties, which can be crucial in developing natural alternatives to traditional antibiotics.

17) Surrounding:
'Surrounding' refers broadly to environmental factors or conditions under which *Turbinaria conoides* grows, such as pollution or other stressors that generate oxidative stress in marine ecosystems. Understanding the surrounding ecosystem is key to comprehending the adaptive mechanisms of the algae and potential for human health benefits.

18) Discussion:
'Discussion' encompasses the analytical part of the research where authors interpret results and relate them to existing literature. This section synthesizes findings, providing context and relevance while suggesting future research directions, emphasizing the importance of scientific dialogue in understanding complex biological phenomena.

19) Attacking:
'Attacking' refers to the action of free radicals on healthy cells, leading to oxidative damage. The document educates on the detrimental effects of free radicals in biological systems and emphasizes the need for antioxidants to neutralize these reactive species, enhancing the understanding of cellular health and protection.

20) Relative:
'Relative' suggests comparisons made between the antioxidant efficiencies of *Turbinaria conoides* and other substances like standard antioxidants (e.g., ascorbic acid). The term emphasizes the importance of establishing a benchmark in scientific studies, which helps illuminate the significance of findings in broader contexts within nutritional and medicinal fields.

21) Harvesting (Harvest):
'Harvest' in this context relates to the collection of bioactive compounds from *Turbinaria conoides*. It indicates the process through which these compounds can be sourced for practical use, particularly in food, health, and pharmaceutical industries, reinforcing the interest in sustainable practices.

22) Cancer:
'Cancer' is mentioned as a significant disease potentially preventable by dietary antioxidants. The research highlights the role of *Turbinaria conoides* in contributing to health through its antioxidant effects, suggesting that compounds in the seaweed may mitigate oxidative stress related to cancer development, enhancing its therapeutic potentials.

23) Nature:
'Nature' refers to the origin of antioxidants derived from natural sources, contrasting synthetic compounds. The emphasis on nature implies a holistic approach to health, encouraging the exploration of naturally occurring substances like *Turbinaria conoides* as alternatives to manufactured chemicals, fostering a better understanding of ecological health.

24) Reason:
'Reason' relates to the underlying rationale for conducting the study—understanding how *Turbinaria conoides* can contribute to health by counteracting oxidative stress. The document stresses the importance of grounding research in logical motivations, driving inquiries that can lead to real-world applications and health benefits.

25) India:
'India' signifies the geographical context of the study, particularly the location from which *Turbinaria conoides* was sourced. This aspect emphasizes the rich biodiversity and vast marine resources in India, underlining the potential of indigenous species in contributing to global health and wellness through traditional knowledge.

26) Aroma (A-roma):
'Aroma' connects to the sensory properties that may be imparted by the phytochemicals within *Turbinaria conoides*. This term signifies the broader applications of marine algae in food flavoring and fragrance, indicating the various aspects of their usability beyond health benefits, enriching culinary experiences.

27) Earth:
'Earth' in the document refers to the global ecosystem that encompasses all living organisms. Mentioning 'Earth' emphasizes the interconnectedness of all biosystems and the importance of biodiversity in supporting life, highlighting the role of natural resources like *Turbinaria conoides* in sustaining human health.

28) Genu:
'Genu' indicates the plural form of genus in biological classification, pertaining to the significance of categorizing *Turbinaria* correctly. Acknowledging genus is crucial for understanding evolutionary relationships, ecological niches, and the specific characteristics that define the bioactive potential of a species.

29) Rich (Rch):
'Rich' describes the abundance of phytochemicals within *Turbinaria conoides*. This term emphasizes the potential of the algae to provide a plethora of bioactive compounds with health benefits, reinforcing the notion of harnessing natural resources for their diverse and beneficial properties in health and nutrition.

30) Hand:
'Hand' signifies the manual processes involved in preparing the extracts, such as shaking the conical flasks to facilitate extraction. This emphasizes the practical, hands-on methods that researchers employ in laboratory settings, highlighting human involvement in experimental setups and the importance of technique in scientific inquiry.

Other Science Concepts:

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Discover the significance of concepts within the article: ‘In vitro antioxidant efficacy of turbinaria conoides’. Further sources in the context of Science might help you critically compare this page with similair documents:

Flavonoid, Phytochemical, Chronic disease, Research Scholar, Pharmaceutical industry, Antioxidant activity, Phytochemical screening, Oxidative stress, Reactive oxygen species, Total phenolic content, Total antioxidant activity, Antioxidant, In vitro antioxidant activity, Reducing power assay, Reducing power, Alkaloids flavonoids, Methanolic extract, Free radical-scavenging assay, Methanol extract, Tannin, 1, 1-diphenyl-2-picrylhydrazyl (DPPH), DPPH radical scavenging activity, Food supplement, Free Radical, Saponin, Superoxide scavenging activity, Terpenoid, DPPH radical, Natural antioxidant, Iron chelating activity, Healthy Cells, Synthetic antioxidant.

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