Reversible effects of Jussiaea repens on rat testes architecture and function.

| Posted in: Health Sciences Science Journals

Journal name: World Journal of Pharmaceutical Research
Original article title: Reversible action of jussiaea repens (l) induced alterations of histoarchitechture vis-à-vis functions in testicular tissues of rat
The WJPR includes peer-reviewed publications such as scientific research papers, reports, review articles, company news, thesis reports and case studies in areas of Biology, Pharmaceutical industries and Chemical technology while incorporating ancient fields of knowledge such combining Ayurveda with scientific data.
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Original source:

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Author:

Subhasish Ghosal, Indrani Chakraborty and Nirmal Pradhan


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World Journal of Pharmaceutical Research:

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Full text available for: Reversible action of jussiaea repens (l) induced alterations of histoarchitechture vis-à-vis functions in testicular tissues of rat

Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research

Copyright (license): WJPR: All rights reserved


Summary of article contents:

Introduction

The study investigates the effects of the non-toxic herb Jussiaea repens L. on testicular histoarchitecture and hormonal functions in male rats. Previous research has identified Jussiaea repens as an antigonadal herb that impacts sperm morphology and decreases fertility. This study aims to clarify whether oral administration of crude aqueous extract affects the normal structure and function of testicular tissue and whether these effects are reversible upon treatment cessation.

Impact on Histoarchitecture

The administration of Jussiaea repens resulted in significant changes to testicular tissue structure, particularly in the seminiferous tubules. After 28 days of treatment at a dose of 200 mg/kg body weight, notable alterations were observed, including reductions in seminiferous tubular and Leydig cell nuclear diameters. Histological analyses indicated a marked decrease in Sertoli cells and various spermatogenic cell types indicative of compromised spermatogenesis. Post-treatment recovery showed a restoration of testicular architecture, further supporting the herb's reversible effects on tissue integrity.

Biochemical Alterations

In addition to histological changes, Jussiaea repens treatment led to significant biochemical modifications in the testicular tissue. There were decreases in testicular sialic acid, phospholipid, and reduced glutathione levels, suggesting impaired metabolic functions crucial for maintaining sperm and testicular health. While serum lipid levels remained stable, an insignificant increase in malondialdehyde (MDA) levels indicated some oxidative stress. Encouragingly, these biochemical parameters normalized during the recovery phase, suggesting the herb's effects are not permanent.

Hormonal Profile Changes

The study documented substantial changes in reproductive hormone levels, with marked reductions in serum testosterone, luteinizing hormone (LH), and follicle-stimulating hormone (FSH) observed in treated rats. These hormonal changes were closely correlated with the alterations seen in testicular histoarchitecture and cellular makeup, leading to disrupted spermatogenic processes. Upon withdrawal of the herb, hormone levels began to restore, further indicating that the hormonal suppression was reversible.

Conclusion

Oral administration of Jussiaea repens L. significantly alters testicular histoarchitecture, biochemical parameters, and reproductive hormone profiles in male rats, resulting in disrupted spermatogenesis and decreased fertility rates. However, the effects of the herb are reversible following the cessation of treatment, making it a potential candidate for development into an herbal contraceptive method. Overall, the research highlights the importance of understanding herbal treatments' short-term and long-term implications on reproductive health.

FAQ section (important questions/answers):

What is the main focus of the study on Jussiaea repens?

The study investigates the effects of the crude aqueous extract of Jussiaea repens on testicular histoarchitecture and functions, particularly focusing on its influence on spermatogenesis and hormone levels in male rats.

How was the extract of Jussiaea repens prepared for the study?

The extract was prepared by boiling dried powdered Jussiaea repens in distilled water, filtering the mixture, concentrating it using a rotary evaporator, and then storing it for further use.

What dosage of the extract was administered to the rats?

Rats were administered a dose of 200 mg of the aqueous extract per kg of body weight per day for 28 consecutive days.

What effects did the extract have on hormone levels?

The extract significantly reduced serum levels of testosterone, LH, and FSH in the treated rats, impacting their reproductive hormone profile.

Were the effects of Jussiaea repens treatment reversible?

Yes, the study concluded that the alterations caused by Jussiaea repens were reversible after the treatment was withdrawn, with hormonal and histological parameters returning towards normal.

What histological changes were observed in the testicular tissues?

Histological analysis revealed distortion and alterations in seminiferous tubules and a reduction in various spermatogenic cells, indicating impaired spermatogenesis in the treated rats.

Glossary definitions and references:

Scientific and Ayurvedic Glossary list for “Reversible effects of Jussiaea repens on rat testes architecture and function.”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.

1) Study (Studying):
The term 'study' refers to a systematic investigation into a specific subject, in this case, the effects of Jussiaea repens on testicular histoarchitecture and functions in male rats. It encompasses various scientific methods and analyses to derive conclusions about the plant's influences on fertility and hormonal changes.

2) Animal:
'Animal' in this context signifies the use of live rats, specifically adult male albino rats (Rattus norvegicus L.), for experimental purposes. They serve as biological models to understand the effects of the Jussiaea repens extract on reproductive parameters and provide insights into potential human applications.

3) Table:
'Table' refers to organized data representations within the study, summarizing results such as biochemical parameters and histological observations. Tables present comparative analyses across different groups (control, treated, and recovery) to enhance clarity and understanding of the effects of the herb on male rat fertility.

4) Water:
In the context of the study, 'water' is crucial as a solvent used to prepare the plant extract. The crude aqueous extract of Jussiaea repens was created using boiled distilled water, indicating the extraction method for the bioactive components believed to affect reproductive health in the study.

5) Male:
The term 'male' identifies the gender of the rats used in the experiment, emphasizing the study's focus on male reproductive physiology. It highlights the specific aspect of fertility, hormonal levels, and testicular functions that are being investigated in the context of potential male contraceptive applications.

6) Activity:
'Activity' is used here to describe the physiological responses and changes observed in the rats as a result of Jussiaea repens administration. This includes alterations in reproductive hormone levels, sperm quality, and histological structure, reflecting the herb's potential pharmacological effects.

7) India:
'India' indicates the geographical location where Jussiaea repens was collected for the study, specifically in West Bengal. It situates the research within the context of local ethnobotanical knowledge and possibly highlights the medicinal use of the plant in traditional Indian medicine practices.

8) Pradhan:
'Pradhan' refers to the lead author and corresponding author of the study, Dr. Nirmal Pradhan. Their involvement signifies the scientific expertise and responsibility for the research, indicating the contributions made toward investigating and publishing findings related to Jussiaea repens and male fertility.

9) Forest:
'Forest' can relate to the natural habitat where Jussiaea repens may be found, emphasizing the ecological context of the study. Since the plant is a water-loving species, its growing conditions might influence its medicinal properties and active compounds, worthy of consideration in the research.

10) Filling (Filled):
In the study, 'filled' usually describes the state of the seminiferous tubules in the testes of the rats, specifically referring to the observation of spermatozoa present or absent in the tubules. The degree of filling can indicate the health and functional capacity of the sperm-producing structures.

11) Arrow:
The term 'arrow' could refer to graphical or illustrative annotations in figures or microscopy images that point to specific structures or findings. This visual aid enhances clarity in presentations of data, allowing readers to quickly identify critical elements relevant to the study's outcomes.

12) Blood:
'Blood' is significant in the research, especially considering the collection of serum samples for hormonal assays. It indicates the biochemical analysis performed to monitor hormonal changes related to testosterone, LH, and FSH levels, which are critical indicators of reproductive function.

13) Antibiotic (Antibacterial):
'Antibacterial' implies that Jussiaea repens has been reported to possess medicinal properties, including antimicrobial effects. This characteristic adds depth to understanding the plant’s various biological roles and potential therapeutic benefits, situating it amongst other traditionally used herbal remedies for health problems.

14) Measurement:
'Measurement' pertains to the quantification of specific biological parameters in the study, including hormonal levels and spermatogenic cell counts. Accurate measurements are crucial for determining the extent of effects induced by the treatment, allowing for statistical analysis and valid conclusions.

15) Observation:
Observation revolves around systematically documenting and analyzing the physical changes in testicular tissues and reproductive health of rats during the study. Careful observation enables the researchers to identify patterns, correlations, and effects of Jussiaea repens on male fertility.

16) Discussion:
The 'Discussion' section provides a critical analysis of the results, where authors interpret their findings in relation to existing literature. It connects study outcomes with broader implications for reproductive health and potential applications of Jussiaea repens in male contraceptive methods.

17) Developing:
'Developing' refers to the processes associated with the maturation of sperm cells within the seminiferous tubules. Understanding how Jussiaea repens influences these developmental stages aids in elucidating its effects on reproductive health and potential fertility impairment.

18) Medicine:
'Medicine' pertains to the context of the research as it touches on pharmacological applications, considering the use of Jussiaea repens as a potential treatment for fertility control. This highlights the intersection of traditional practices and modern medicinal research.

19) Fixation:
'Fixation' in the study context is a technique related to preparing tissue samples for histological examination. It preserves cellular structures, allowing for accurate microscopic analysis and ensuring that the observed histoarchitectural features remain stable during testing.

20) Relative:
The term 'relative' may indicate comparisons made between treatment groups or among observed parameters, such as hormonal levels and histological changes. It highlights the importance of analyzing variations in context to establish comprehensive findings relevant to the impacts of treatment.

21) Indrani (Imdrani):
'Indrani' refers to one of the co-authors of the research, Indrani Chakraborty, signifying collaborative effort in conducting the study. Her contribution adds scientific rigor and diversity of expertise to the project, enhancing the overall quality of the research findings.

22) Quality:
'Quality' refers to the standard of sperm or testicular function measured in the study, including parameters like sperm morphology and viability. Understanding the quality dimensions is essential for evaluating the herb's contraceptive potential and reproductive health implications.

23) Disease:
In this context, 'disease' might refer to health conditions related to infertility or reproductive dysfunctions. Investigating Jussiaea repens can contribute to understanding and addressing male reproductive diseases, offering insights for herbal treatments in fertility preservation.

24) Science (Scientific):
'Science' is the overarching field that encompasses the methodical study conducted in this research. It represents the disciplined approach to understanding biological and medical principles, emphasizing evidence-based conclusions drawn from the experiments on Jussiaea repens.

25) Surface:
'Surface' may refer to the morphology of the plant or specific structural features within the testicular tissues observed during histological or electron microscopic examinations. This term is crucial for understanding the detailed anatomy and functions examined in the study.

26) Bengal (Bemgal):
'Bengal' designates the specific region in India where the study took place, emphasizing its botanical and cultural significance. The environmental context can relate to the availability of Jussiaea repens and its traditional uses in local medicine.

27) Muslin:
'Muslin' denotes the type of cloth used for filtering the plant extract during preparation. This detail indicates the methods and materials utilized in extracting bioactive components, highlighting an essential step in the scientific process employed in the study.

28) Family:
'Family' relates to the botanical classification of Jussiaea repens within the Onagraceae family. This classification aids in contextualizing the plant within other related species, which may share similar properties or efficacy, enriching the understanding of its medicinal value.

29) Street:
'Street' appears to reference the specific location related to one of the authors' affiliations or the setting of the research institution. It helps provide an address and institutional context for the collaboration involved in conducting the research.

30) Powder:
'Powder' refers to the form of the plant material that was used for extraction. The dried powdered form of Jussiaea repens indicates the method of preparing the plant for subsequent aqueous extraction to analyze its potential biological effects.

31) Diet:
'Diet' indicates the standard food provided to the rats during the study. A controlled diet ensures consistent nutritional availability for the animals, eliminating dietary variations that could influence the research results on the effects of the plant extract.

32) Gold (Golden):
'Gold' may refer to the coating used during the scanning electron microscopy (SEM) process for imaging the testicular tissues, enhancing clarity and resolution. This technique is important for observing structural details at the microscopic level, vital for accurately assessing the study's findings.

33) Post:
'Post' can refer to the period following the treatment of the rats, particularly regarding the recovery phase. This aspect is essential for assessing the reversibility of the changes induced by Jussiaea repens upon cessation of the treatment protocol.

Other Science Concepts:

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Discover the significance of concepts within the article: ‘Reversible effects of Jussiaea repens on rat testes architecture and function.’. Further sources in the context of Science might help you critically compare this page with similair documents:

Seminal fluid, Oral administration, Experimental group, Animal Ethics Committee, Reduced glutathione, Aqueous extract, Spermatogenesis, Follicle Stimulating Hormone (FSH), Microsomal lipid peroxidation, Malondialdehyde (MDA), Serum testosterone, Biochemical alteration, Recovery group, Toxic effect, Leydig cells, Male contraceptive, Testicular histology, Pharmaceutical Research, Histological structure, Serum testosterone level, Testosterone Level, Luteinizing hormone (LH), Testicular dysfunction, Histological analysis, Glutathione depletion, Spectrophotometer reading, Leydig cell function, Histoarchitecture, ELISA method, Biochemical parameter, Morphological change, Hormonal profile, GSH activity, MDA level, Male reproductive system, Acridine orange staining, Testicular testosterone, Controlled laboratory conditions, Spermatogenesis process, Spermatogenic cell, Sperm chromatin structure, Statistical Analysis of Data, Optical Densities, Testicular tissue, Pyknotic nuclei, Testicular architecture, Standard laboratory condition, Infertility rate, Colorimetric determination, Spermatozoa maturation, Cell population dynamics, Testicular function, Critical point dryer, Germinal cells, Plant extract treatment, Sperm DNA integrity, Seminiferous tubule, Sertoli cell, Tissue sulfhydryl groups, Sialic acid content, Spermatogenesis stages, Interstitial cells, Crude aqueous extract, Hormone profile, Clinical guide, Glutathione related enzymes.

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