Botanical and genetic traits of Dalbergia paniculata in Egypt.

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Journal name: World Journal of Pharmaceutical Research
Original article title: Botanical and genetic characteristics of dalbergia paniculata roxb. cultivated in egypt
The WJPR includes peer-reviewed publications such as scientific research papers, reports, review articles, company news, thesis reports and case studies in areas of Biology, Pharmaceutical industries and Chemical technology while incorporating ancient fields of knowledge such combining Ayurveda with scientific data.
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Original source:

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Author:

Amira K. El-Motayam , Dina M. R. Abou-Hussein , Soad H. Tadros and Elsayed A. Aboutabl


World Journal of Pharmaceutical Research:

(An ISO 9001:2015 Certified International Journal)

Full text available for: Botanical and genetic characteristics of dalbergia paniculata roxb. cultivated in egypt

Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research


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Summary of article contents:

1) Introduction

The genus Dalbergia, belonging to the Fabaceae family, includes around 159 species found predominantly in tropical and subtropical regions worldwide. Among these, Dalbergia paniculata Roxb. is a non-climbing evergreen tree notable for its yellowish white flowers and valuable heartwood used in instruments and construction. This species exhibits a variety of medicinal activities, such as antimicrobial and antioxidant properties. The study conducted presents detailed macro- and micro-morphological characteristics of Dalbergia paniculata and explores its genetic profiling through DNA fingerprinting techniques, aiming to improve its identification and differentiation from closely related species such as Dalbergia sisso Roxb., which is also found in Egypt.

2) Morphological Profiling of Dalbergia paniculata***
The morphological analysis of Dalbergia paniculata involves studying various plant organs, including the stem, leaves, fruits, and seeds. The tree is described as medium to large, with a height of 12-17 m and a hard woody trunk covered in dark, scaly bark. The leaves are alternate and compound, comprising 11-15 leaflets with a leathery texture and prominent venation. The fruit is presented as short-stalked legumes, and the seeds are kidney-shaped and glossy. This detailed macromorphological characterization aids in identifying the species in its whole and ground forms, essential for both botanical research and practical applications in medicinal uses.

3) Micromorphological Characteristics

The study's micromorphological investigations delve into the cellular structure of various parts, particularly the stem and leaves. The old stem's transverse section reveals a complex arrangement of cork, cortex, and vascular tissue, while the young stem shows slightly varying features, such as a thinner cork layer and differing types of trichomes. The leaves display a dorsiventral mesophyll and prominent vascular bundles, while also revealing the distinct structure of upper and lower epidermises. The microscopic details contribute significantly to understanding the anatomical features of Dalbergia paniculata, which can assist in its identification and classification within the family Fabaceae.

4) Genetic Profiling Using RAPD Analysis

The genetic profiling of Dalbergia paniculata used Random Amplified Polymorphic DNA (RAPD) techniques to examine genetic variability between it and Dalbergia sisso. A total of 15 decamer primers were employed, producing 179 fragments for D. paniculata and 156 for D. sisso. The analysis provided reproducible band patterns, demonstrating that both species could be differentiated effectively based on their genetic markers. This genetic fingerprinting serves as a powerful tool for authenticating medicinal plant species, revealing the potential for future applications in biodiversity studies and conservation efforts.

5) Conclusion

The study emphasizes the significance of both morphological and genetic profiling in understanding and distinguishing Dalbergia paniculata. The comprehensive analysis showcases the plant's valuable attributes and medicinal potential while demonstrating effective methodologies for species identification. The findings underscore the importance of combining botanical studies with genetic techniques to enhance the authentication of medicinal plants, thereby contributing to both scientific research and practical applications in herbal medicine and conservation. Utilizing the RAPD technique offers promising future directions for exploring genetic diversity and establishing conservation strategies for valuable species within the Fabaceae family.

FAQ section (important questions/answers):

What is the botanical significance of Dalbergia paniculata?

Dalbergia paniculata is a perennial tree with medicinal properties, used traditionally for treating various ailments including infections and inflammation. Its heartwood is valuable for making musical instruments and construction, and it exhibits significant antimicrobial and antioxidant activities.

How many species of Dalbergia are found in Egypt?

In Egypt, there are two species of Dalbergia: Dalbergia paniculata and Dalbergia sisso. Both species are notable for their timber, which is highly sought after for its decorative and fragrant qualities.

What methods were used for genetic profiling of Dalbergia paniculata?

Genetic profiling was conducted using Random Amplified Polymorphic DNA (RAPD) analysis, employing 15 decamer primers. This method produced 179 DNA fragments that helped differentiate Dalbergia paniculata from Dalbergia sisso.

What are the macromorphological features of Dalbergia paniculata?

Dalbergia paniculata exhibits a cylindrical trunk, dark rough bark, and compound leaves comprised of 11-15 leaflets. The tree flowers in March, and its fruits are elliptical legumes that ripen from green to yellowish brown.

What are the common uses of Dalbergia paniculata?

The heartwood of Dalbergia paniculata is primarily used in making musical instruments and construction. Additionally, it is known for its medicinal properties, being used for antimicrobial, anti-inflammatory, and antioxidant purposes in traditional medicine.

What are the results of the RAPD analysis for Dalbergia paniculata?

The RAPD analysis revealed a total of 179 fragments for Dalbergia paniculata and 156 for Dalbergia sisso. The study indicated a polymorphism rate of 61.61%, allowing for effective identification and authentication of these species.

Glossary definitions and references:

Scientific and Ayurvedic Glossary list for “Botanical and genetic traits of Dalbergia paniculata in Egypt.”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.

1) Wall:
In botany, the term 'wall' often refers to the cellular structure that makes up the boundaries of plant cells. The wall is essential for maintaining structural integrity, protection against pathogens, and regulating water movement, contributing to the overall physiology of the plant, particularly in species like Dalbergia paniculata.

2) Surface:
The 'surface' of a plant refers to the outermost layer of its organs, such as leaves and stems. This layer plays a critical role in facilitating gas exchange, protecting against water loss, and providing defense mechanisms against herbivory and pathogens. The microstructure of the surface is evidenced through microscopic examinations.

3) Species:
'Species' is a fundamental concept in biological classification, representing a group of organisms that can interbreed and produce fertile offspring. The research focuses on two species from the genus Dalbergia—Dalbergia paniculata and Dalbergia sisso—emphasizing their morphological and genetic characteristics and potential uses in medicine and construction.

4) Egypt:
'Egypt' is the geographical location where the study of Dalbergia paniculata was conducted. It highlights the significance of this region in biodiversity and the presence of specific plant species. The cultivation of local species has implications for traditional medicine and economic activities, such as timber production.

5) Table:
In scientific research, 'table' refers to a systematic arrangement of data often used to summarize findings, such as microscopical measurements or RAPD markers. Tables facilitate comparison and easy interpretation of quantitative results, ensuring clarity and enhancing the presentation of complex information relevant to plant studies.

6) Tree:
'Tree' refers to a perennial woody plant, typically characterized by a singular trunk supporting branches. The study focuses on Dalbergia paniculata, a medium to large tree known for its valuable heartwood. Understanding its characteristics contributes to knowledge about forestry, timber uses, and ecological significance.

7) Measurement:
'Measurement' is the quantitative assessment of various plant characteristics, such as dimensions of cells and structures in the studied species. Accurate measurements are vital in botany to establish norms for morphological analysis and to provide comparative data crucial for identifying species and their potential applications.

8) Filling (Filled):
The term 'filled' often describes the anatomical features of plant cells, particularly regarding their contents. For example, the cotyledons of seeds might be filled with oils and proteins, which are critical for the nutrition of developing plants. This aspect influences growth patterns and applications in agriculture.

9) Fainting (Fainted):
'Faint' is used to describe the subtle characteristics of plant parts, such as a faint characteristic odor. This term can be significant in pharmacognosy to identify and authenticate plant species, as aromatic properties can indicate the presence of specific phytochemicals linked to medicinal activities.

10) Surrounding:
The term 'surrounding' refers to the protective or encompassing structures around plant tissues or organelles. This can include layers of cells around a vascular bundle. Understanding surrounding tissues is essential for exploring physiological roles, growth conditions, and the plant's structural integrity against environmental stress.

11) Similarity:
'Similarity' signifies the degree to which two species share genetic or morphological traits. This concept is crucial in the study of Dalbergia species, aiding in understanding their evolutionary relationships, differentiation, and potential uses in various applications, including medicinal and ecological contexts.

12) Bitter:
The term 'bitter' relates to sensory properties of certain plant parts, particularly in pharmacognosy. The bitterness of some plant extracts indicates the potential presence of bioactive compounds that can be useful. Such sensory qualities often guide traditional uses in medicine and food preparation, particularly in herbal remedies.

13) Study (Studying):
'Study' is fundamental to the scientific method, representing the systematic investigation into the properties and characteristics of Dalbergia paniculata. This encompasses botanical profiling, microscopical examinations, and genetic analysis, aimed at expanding knowledge for potential applications in medicine, timber production, and conservation.

14) Genu:
The term 'genus' refers to a taxonomic rank that groups species sharing common characteristics. Dalbergia is the genus containing various species of trees used for timber and medicinal purposes. Understanding the genus is essential in botanical classification and for exploring ecological relationships within this family.

15) Depression:
'Depression' in botanical terms may refer to features on seeds or leaves, such as indentations near the hilum. Such morphological characteristics can be important for species identification and classification, as they provide key diagnostic features in distinguishing between similar species in the Dalbergia genus.

16) Medicine:
'Medicine' refers to the field concerned with health and healing. The study highlights the medicinal properties of Dalbergia paniculata, which has reported activities such as antimicrobial and antioxidant effects. Understanding medicinal plants contributes to developing new therapeutic agents and healthcare practices in traditional settings.

17) Street:
'Street' in this context refers to Kasr El-Einy Street, where the research institution is located. It demonstrates the importance of specific geographical locations in botanical studies, often associated with the availability of native plants, making it vital for researchers and educators in pharmacognosy and related fields.

18) Powder:
'Powder' describes the form of plant material after grinding, essential for preparing extracts used in analysis. The powdered form of Dalbergia paniculata facilitates microscopic analysis and the extraction of bioactive compounds necessary for pharmacological studies and quality control in herbal medicines.

19) Amira:
'Amira' refers to one of the authors of the study, signifying the contribution of researchers in advancing knowledge in pharmacognosy and botany. The work done by authors like Amira K. El-Motayam is critical in providing scientific rigor and innovative findings regarding plant species and their applications.

20) Ripe:
'Ripe' refers to the state of fruit maturity, impacting the availability of seeds for research. Understanding the ripening process is crucial in botanical studies, as it signifies the optimal time for collection, influencing the quality of genetic and phytochemical analysis in species like Dalbergia paniculata.

21) Discussion:
'Discussion' is a critical component in scientific papers, summarizing findings and providing interpretations of results. This section allows researchers to connect their work to existing literature, highlight implications, and suggest future research directions, particularly concerning the medicinal and ecological significance of studied species.

22) Activity:
'Activity' relates to the biological effects exhibited by plant extracts, such as antioxidative or antimicrobial properties. In the context of Dalbergia paniculata, activity assessments are crucial for validating traditional uses in medicine and exploring new avenues for pharmaceuticals, contributing to drug discovery efforts.

23) Bleeding:
'Bleeding' denotes a critical medical condition often treated with medicinal plants. The research discusses the traditional use of Dalbergia species for ailments such as bleeding piles, associating botanical research with practical applications in addressing health concerns and potentially identifying bioactive compounds beneficial for healing.

24) Leprosy:
'Leprosy' is a chronic infectious disease, historically addressed by various medicinal plants in traditional therapies. The study emphasizes the involvement of Dalbergia species in treating such conditions, highlighting the significance of ethnobotanical research in discovering effective treatments and validating traditional claims through scientific investigation.

25) Mineral:
'Mineral' refers to inorganic constituents that may be found in plant tissues. In the study of Dalbergia paniculata, minerals like calcium oxalate are noted for their role in plant structure and physiology. Understanding mineral composition aids in comprehending plant mechanisms and their adaptations to environmental conditions.

26) Gelatin:
'Gelatin' is referenced in the context of gel electrophoresis, a technique used to separate DNA fragments. Its role is crucial in molecular biology for visualizing the results of genetic profiling. Understanding these techniques aids in the analysis of genetic variability among species like Dalbergia.

27) Family:
'Family' is a higher taxonomic rank that groups related genera. In this context, Fabaceae is the family containing Dalbergia species. Understanding family classifications supports the examination of evolutionary relationships, aiding in the study of plant morphology, ecology, and potential uses in agriculture and medicine.

28) Timber:
'Timber' denotes wood that is processed for construction and other applications. Dalbergia paniculata is prized for its heartwood, contributing to economic activities. The study underscores the importance of sustainable timber management and the ecological significance of temperate hardwood species in forestry practices.

29) Medium:
'Medium' indicates the size or scale of an organism, particularly in describing Dalbergia paniculata as a medium to large tree. Understanding the medium size is relevant in assessing its habitat requirements, growth potential, and comparative studies against other tree species in similar environments.

30) Botany:
'Botany' is the scientific study of plants, encompassing various aspects such as morphology, genetics, and ecology. The research on Dalbergia paniculata contributes to botany by enhancing knowledge on plant structures, genetic variability, and their applications in medicine and environmental conservation.

31) Water:
'Water' is a critical component for plant survival, influencing growth and physiological processes. Its availability affects the distribution and health of Dalbergia paniculata, emphasizing the importance of studying water relations in understanding the adaptability and ecological roles of this species in its native habitat.

32) Ulcer:
'Ulcer' refers to a medical condition that can be treated with herbal remedies. The study highlights the traditional use of Dalbergia species for treating ulcers, connecting historical medicinal practices with current research aimed at validating the efficacy of such treatments in contemporary medicine.

33) Cutan:
'Sudan' may refer to Sudan III, a staining reagent used to visualize lipids within plant tissues. In examining Dalbergia paniculata, this method illustrates the presence of specific compounds in the seed, thereby shedding light on its nutritional and pharmacological properties and potential health benefits.

34) Dina:
'Dina' refers to one of the authors of the study, contributing to the body of knowledge regarding Dalbergia paniculata. The collaboration among authors illustrates the interdisciplinary nature of research in pharmacognosy, genetics, and botany, fostering advances in understanding plant biology and its applications.

35) Cetu:
'Cetus' is referenced regarding the type of thermocycler used in PCR processes for genetic analysis. This equipment is essential for amplifying DNA, crucial in conducting the RAPD analysis of Dalbergia species, providing insights into genetic diversity and species authentication through molecular techniques.

36) King:
'King' refers to King Abdulaziz University, where one of the authors is affiliated. This connection highlights the academic and research networks fostering studies in pharmacognosy and botany, emphasizing collaboration in advancing research in ethnobotanical and medicinal plants in Saudi Arabia.

37) Hair:
'Hair' pertains to the non-glandular trichomes on plant surfaces. These structures have adaptive functions, such as reducing water loss or deterring herbivory. Understanding hair morphology is essential in exploring the ecological relationships and potential uses of plant species like Dalbergia paniculata.

38) Line:
'Line' can represent various contexts in botanical research, including lineage, lineage tracing in genetics, or the demarcation of observations or discussions in research reports. In the study of Dalbergia paniculata, lines of inquiry connect observations on morphology and genetic identification for comprehensive understanding.

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Discover the significance of concepts within the article: ‘Botanical and genetic traits of Dalbergia paniculata in Egypt.’. Further sources in the context of Science might help you critically compare this page with similair documents:

Traditional medicine, Antimicrobial activities, Medicinal plant species, Pharmacognosy, Antioxidant activities, Morphological character.

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