Phytochemical screening and antioxidant evaluation of Dendrobium ochreatum.
Journal name: World Journal of Pharmaceutical Research
Original article title: Phytochemical screening and evaluation of anti oxidant activity of different parts of dendrobium ochreatum.
The WJPR includes peer-reviewed publications such as scientific research papers, reports, review articles, company news, thesis reports and case studies in areas of Biology, Pharmaceutical industries and Chemical technology while incorporating ancient fields of knowledge such combining Ayurveda with scientific data.
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Janmajoy Banerjee, Biplab Kumar Dey, Hemanta Khanal, Bibek Dahal
World Journal of Pharmaceutical Research:
(An ISO 9001:2015 Certified International Journal)
Full text available for: Phytochemical screening and evaluation of anti oxidant activity of different parts of dendrobium ochreatum.
Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research
Copyright (license): WJPR: All rights reserved
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Summary of article contents:
Introduction
The study focuses on the evaluation of phytoconstituents and the antioxidant activities of various extracts from the plant species Dendrobium ochreatum, an orchid belonging to the family Orchidaceae. With a rich biodiversity of about 25,000 species, orchids are primarily appreciated for their ornamental value; however, their medicinal properties have not been extensively explored. The research examines nine different solvent extracts, specifically targeting antimicrobial and antioxidant properties, thus contributing to the academic understanding of this understudied plant.
Phytochemical Screening
The preliminary phytochemical analysis indicated a presence of various bioactive compounds across the different extracts of Dendrobium ochreatum. In particular, the ethanolic extract of the root, stem, and the hexane-ethanol extract from the stem demonstrated a rich profile of phytochemicals including steroids, alkaloids, and tannins. The results emphasize the capability of ethanol as an effective solvent for extracting a broader range of phytoconstituents, suggesting potential therapeutic applications.
Antioxidant Activity Using DPPH Assay
The study employed the DPPH radical scavenging assay to assess antioxidant activity. It's a widely recognized method where antioxidants reduce DPPH, a stable radical, leading to a decrease in absorbance measured at 517 nm. The results revealed significant antioxidant activities in specific extracts, notably the ethyl acetate extract of the stem and the ethanolic extract of the root, exhibiting moderate radical scavenging activities with IC50 values of 172.27 μg/ml and 132.43 μg/ml, respectively. This suggests that these extracts could potentially mitigate oxidative stress.
ABTS Radical Scavenging Assay
Additionally, the research explored the antioxidant capacity using the ABTS radical scavenging assay. Similar to the DPPH assay, this method revealed that the ethyl acetate extract of the stem and ethanolic extract of the root showed commendable inhibition of ABTS radical activity, with IC50 values of 140.06 μg/ml and 123.21 μg/ml respectively. This corroborates findings from the DPPH assay, further highlighting the antioxidant potential of Dendrobium ochreatum and its phytoconstituents, reinforcing the need for further investigations.
Conclusion
In conclusion, the study on Dendrobium ochreatum indicates that the plant has significant antioxidant properties, primarily attributed to its rich phytochemical profile. The results demonstrate that ethanol is particularly effective for extracting bioactive compounds with potential health benefits. This research not only enriches the understanding of Dendrobium ochreatum as a source of natural antioxidants but also paves the way for further studies into its medicinal applications, thereby advocating for the exploration of more orchid species for their therapeutic potentials.
FAQ section (important questions/answers):
What was the main objective of the Dendrobium ochreatum study?
The study aimed to evaluate the phytoconstituents, antimicrobial, and antioxidant activities of different extracts from the stem, root, and leaves of Dendrobium ochreatum.
Which extraction solvents showed maximum phytochemical constituents?
The ethanolic extract of root and stem, along with the hexane-ethanol extract of stem, revealed the highest levels of phytochemical constituents, indicating effective extraction using these solvents.
How was antioxidant activity measured in the study?
Antioxidant activity was measured using DPPH and ABTS radical scavenging assays, which evaluate the ability of extracts to scavenge free radicals and reduce their concentration.
What were the findings regarding DPPH radical scavenging activity?
The ethyl acetate extract of the stem showed moderate DPPH radical scavenging activity (IC50 at 172.27 μg/ml), indicating significant antioxidant potential relative to other extracts.
What phytochemicals were identified in Dendrobium ochreatum extracts?
The study identified compounds such as steroids, alkaloids, flavonoids, glycosides, and tannins across various solvent extracts, suggesting potential health benefits of the plant.
What conclusion was drawn from the study about extract efficacy?
The study concluded that ethanol effectively extracts the highest phytoconstituents, and the antioxidant potential is attributed to flavonoids, alkaloids, and other compounds present in the extracts.
Glossary definitions and references:
Scientific and Ayurvedic Glossary list for “Phytochemical screening and antioxidant evaluation of Dendrobium ochreatum.”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.
1) Activity:
Activity in this context refers to the functional effectiveness of the extracts in performing various biological tasks, particularly their antioxidant capacity. The study seeks to measure how well the extracts can neutralize free radicals, which is significant for determining their potential health benefits and therapeutic applications.
2) Table:
A Table is a structured arrangement of data, used here to organize information such as yield values, percentages of inhibition, and phytochemical constituents present in extracts of Dendrobium ochreatum. Tables help to present complex data clearly for easier comparison, analysis, and interpretation within the research.
3) Species:
Species denotes a specific group of plants or organisms that share common characteristics. In this research, Dendrobium ochreatum is the species under investigation, highlighting its unique properties and chemical composition which may have antioxidant or medicinal applications, thereby contributing to botanical and pharmacological knowledge.
4) Study (Studying):
Study refers to the systematic investigation conducted to assess the phytochemical screening and antioxidant activities of various parts of Dendrobium ochreatum. It encompasses methodology, results, and analysis aimed at expanding the scientific understanding of this plant's potential benefits, especially in the context of pharmaceutical research.
5) Powder:
Powder refers to the processed form of the plant materials, obtained by grinding dried leaves, stems, and roots. Powdered extracts are crucial for subsequent solvent extraction processes in research, as they enhance the surface area available for the extraction of bioactive compounds, facilitating their analysis.
6) Phytochemical:
Phytochemical refers to the natural bioactive compounds present in plants, which may possess health-promoting properties. The study emphasizes the identification and quantification of these compounds in Dendrobium ochreatum, aiming to explore their potential therapeutic effects, such as antioxidant activity, which is a focus of the research.
7) Flavonoid:
Flavonoid is a type of phytochemical known for its antioxidant properties and various health benefits. In this context, the presence of flavonoids in the extracts of Dendrobium ochreatum contributes to its overall antioxidant activity. Their role is crucial in understanding the plant's potential medicinal applications.
8) India:
India indicates the geographical or cultural focus of the research, specifically where the Dendrobium ochreatum plant was sourced. It highlights the regional biodiversity and traditional uses of plants, thus contextualizing the medicinal and nutritional significance of the species within its indigenous landscape.
9) Tirunavukkaracu (Tiru-navukkaracu, Thirunavukkarasu):
Thirunavukkarasu refers to a contributing author or researcher who has worked on related scientific matters, particularly in the area of antioxidant activity assessment. Citing this individual acknowledges their contributions to the field and provides a framework for understanding the methodologies or results presented in this study.
10) Shrinivasa (Shri-nivasa, Srinivasa):
Srinivasa indicates another researcher referenced within the study, contributing evidence or methodology regarding antioxidant activities. Mentioning such authors lends credibility to the research findings and connects the current study to previous work, enhancing the scholarly context and validating the investigation.
11) Campu:
Campu likely refers to a context within educational or research facilities where studies are conducted. In this manuscript, it emphasizes the institutional affiliations of the authors and may influence the credibility and authority of the research conducted on Dendrobium ochreatum.
12) Water:
Water is a vital solvent in the extraction of phytochemicals and is often used in laboratory processes. In this study, it may relate to the methods used for preparing extracts from Dendrobium ochreatum. Understanding solvent selection is critical for effective extraction and analysis of bioactive components.
13) Cina:
China denotes the geographical relevance of the Dendrobium species, which is known to have many varieties in the region. The differential species may offer insights into the unique pharmacological properties attributed to these plants, driving further research into their ecological and medicinal significance.
14) Genu:
Genu refers to a taxonomic classification, specifically the genus, under which Dendrobium falls. Understanding the genus is vital for identifying the plant's relationships with other species, its classification, and its role within the large family of Orchidaceae. This contextualizes the phytochemical exploration of Dendrobium ochreatum.
15) Pharmacological:
Pharmacological pertains to the study of drugs and their effects on biological systems. This term emphasizes the focus of the research on exploring the potential medicinal properties of the extracts derived from Dendrobium ochreatum, linking phytochemical constituents to their therapeutic applications.
16) Pharmacology:
Pharmacology is the scientific discipline that examines the study of drugs, their actions, and interactions with living organisms. This research contributes to the field by investigating the pharmacological properties of Dendrobium ochreatum, aiming to identify its potential as a source of beneficial medicinal compounds.
17) Siddhartha (Siddha-artha):
Siddhartha is a referenced author in the text, contributing to the background knowledge about the use of orchids in medicine. Including his work helps contextualize the study’s results regarding the economic importance and medicinal uses of Dendrobium ochreatum within wider scholarly research.
18) Discussion:
Discussion comprises a section in the research where findings are interpreted, compared, and related to previous studies. This part elaborates on the significance of the antioxidant activity observed in Dendrobium ochreatum, connecting experimental results with existing literature and suggesting future research directions.
19) Hemanta (Hemamta):
Hemanta refers to one of the authors or researchers involved in this study, indicating collaborative efforts in the investigation. Mentioning all authors promotes accountability and gives credit to those contributing to the research, thereby enhancing the integrity of the documented findings.
20) Habitat:
Habitat refers to the natural environment where Dendrobium ochreatum is found and can significantly influence its phytochemical composition. Understanding the habitat provides insights into the ecological factors that contribute to the growth and properties of this species, aiding in conservation and cultivation strategies.
21) Bengal (Bemgal):
Bengal specifies a geographic region from which the Dendrobium ochreatum samples were collected, particularly in India. It highlights the localized nature of the study, showcasing the regional biodiversity and traditional uses of plants, thus contributing to the understanding of floral diversity in the area.
22) Family:
Family denotes the taxonomic rank in the classification of organisms. In this research, it reflects the broader classification of Dendrobium ochreatum within the Orchidaceae family, which is significant for identifying relationships with other species and understanding shared characteristics, including medicinal properties.
23) Reason:
Reason indicates the rationale behind conducting the research and investigating Dendrobium ochreatum's antioxidant activities. It reflects motivations such as the growing interest in natural products as sources for health benefits and addressing gaps in knowledge regarding the effects of this particular species.
24) Filling (Filled):
Filled refers to the process of preparing plant extracts by packing the powdered materials into an extractor for consistent solvent extraction. This term is significant as it emphasizes the methodology that affects the yield and quality of the phytochemicals extracted for further assessment.
25) Kumar:
Kumar is another author contributing to the study, showcasing collaboration in researching Dendrobium ochreatum. Mentioning all authors emphasizes the collective effort in scientific inquiry, helps in acknowledging individual contributions, and enhances the credibility of the research findings.
26) Janaka (Janak):
Janak identifies the specific nursery or source from which the plants were obtained for the study. This detail is important as it lends authenticity to the research by confirming where the specimens were sourced, ensuring that studies can replicate the findings from studied materials.
27) Sho (So):
Shao refers to an author whose work is cited within the context of this research, particularly regarding the immunological or antioxidant properties of phytochemicals. Their inclusion highlights prior investigative findings which lend support to the current study's framework and elucidates relationships with previous research.
28) Pur:
Poor describes the level of effectiveness or yield in performance for certain extracts within the study. This term is relevant as it indicates specific extracts that did not show significant antioxidant activity in comparison to others and informs the overall understanding of the plant's properties.
Other Science Concepts:
Discover the significance of concepts within the article: ‘Phytochemical screening and antioxidant evaluation of Dendrobium ochreatum.’. Further sources in the context of Science might help you critically compare this page with similair documents:
Phytochemical, Antioxidant activity, Preliminary phytochemical screening, Phytochemical screening, Phytochemical analysis, Free radical scavenging, Free radical scavenging activity, DPPH assay, Ethanolic extract, ABTS assay, Analytical grade, Antioxidant activities, IC50 value, Antioxidant potential, Ethanol extract, 1, 1-diphenyl-2-picrylhydrazyl (DPPH), Percentage inhibition, Preliminary phytochemical analysis, Chemical constituent, Phytochemical methods, Hexane extract, Pharmacological mechanism, Himalayan pharmacy institute, Polysaccharide, Solvent extract.