Terpenoids from cassia siamea
Journal name: World Journal of Pharmaceutical Research
Original article title: Terpenoids from cassia siamea
The WJPR includes peer-reviewed publications such as scientific research papers, reports, review articles, company news, thesis reports and case studies in areas of Biology, Pharmaceutical industries and Chemical technology while incorporating ancient fields of knowledge such combining Ayurveda with scientific data.
This page presents a generated summary with additional references; See source (below) for actual content.
Original source:
This page is merely a summary which is automatically generated hence you should visit the source to read the original article which includes the author, publication date, notes and references.
Deepa Chauhan and J. Singh
World Journal of Pharmaceutical Research:
(An ISO 9001:2015 Certified International Journal)
Full text available for: Terpenoids from cassia siamea
Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research
Copyright (license): WJPR: All rights reserved
Download the PDF file of the original publication
Summary of article contents:
Introduction
The research article discusses the isolation and structural elucidation of two new triterpenoids derived from the plant Cassia siamea, a member of the fabaceae family predominantly found in Northern India. Known for its medicinal properties, this plant has active compounds contributing to various therapeutic effects, including antimicrobial, antidiabetic, and anticancer activities. This study aims to provide insights into the chemical composition of Cassia siamea and evaluate the significance of its triterpenoids and other secondary metabolites.
Novel Triterpenoids Isolated
Compound 1: Structural Highlights
One of the new compounds identified is 2-oxo-1β, 3β, 19α-trihydroxyurs-12-ene-28-oic acid-β-D-glucopyranoside, referred to as Compound 1. It was isolated as platelet crystals and characterized through various spectral analyses, demonstrating distinct functional groups such as hydroxy and carbonyl groups. The compound’s structure was confirmed using NMR (Nuclear Magnetic Resonance) data, revealing a glucosyl moiety attached at C-28, suggesting its classification as a 19α-hydroxy urs-12-ene type of triterpenoid.
Another Unique Triterpenoid
Compound 2: Chemical Features and Analysis
The second triterpenoid identified, 1β, 2α, 3β, 19α-tetrahydroxyurs-12-ene-28-oate-3-0-β-D-glucopyranoside, or Compound 2, was characterized by its melting point and chemical components, indicating a similar glucosyl attachment. The mass spectral data provided insights into the compound's fragmentation patterns and structural features, with significant peaks correlating to its hydroxyl and carbonyl functional groups. The integration of spectral data, including IR and NMR, allowed for a thorough confirmation of its molecular structure.
Extraction and Isolation Methods
Isolation Techniques Employed
The extraction process involved air-drying and crushing the stem bark of Cassia siamea, followed by exhaustive extraction using boiling ethanol. Chromatography over silica gel G was utilized, yielding the two isolated compounds, which were rigorously analyzed through IR and NMR spectrographs. This methodological approach underscores the importance of proper extraction techniques in identifying active compounds from plant materials, supporting further research into their pharmacological potential.
Conclusion
The study established that Cassia siamea is a rich source of novel triterpenoids, which hold promise for medicinal applications due to their diverse biological activities. The elucidation of structures for the identified compounds, along with their extraction and characterization, contributes to the growing knowledge of plant-derived therapeutic agents. Continued research into the pharmacological efficacy of triterpenoids from Cassia siamea could pave the way for developing new treatments for various diseases. The findings from this study enhance the understanding of the chemical biodiversity present in this plant and encourage further exploration of its medicinal uses.
FAQ section (important questions/answers):
What triterpenoids were isolated from Cassia siamea in the study?
The study isolated two new triterpenoids: 2-oxo-1β, 3β, 19α-trihydroxyurs-12-ene-28-oic acid-β-D-glucopyranoside and 1β, 2α, 3β, 19α-tetrahydroxyurs-12-ene-28-oate-3-0-β-D-glucopyranoside from Cassia siamea.
What medicinal properties does Cassia siamea exhibit?
Cassia siamea has shown antimicrobial, antidiabetic, anticancer, hypotensive, and antioxidant properties, among others. Its decoction is used for various ailments, including diabetes and urogenital diseases.
How were the triterpenoids extracted from Cassia siamea?
The triterpenoids were extracted from air-dried and crushed material using boiling ethanol. The extract was then chromatographed over a dry flash column resulting in the isolation of the compounds.
What techniques were used for the structural elucidation of the compounds?
The structural elucidation of the compounds involved techniques like IR spectroscopy, NMR spectroscopy, mass spectral analysis, and chemical hydrolysis to determine their molecular structures and functional groups.
What was the melting point of Compound 1?
The melting point of Compound 1 was found to be 140-142°C, indicating its stable crystalline form as isolated from Cassia siamea's extracts.
What components were identified in the spectral analysis of Compound 1?
The spectral analysis of Compound 1 indicated the presence of hydroxy groups, ester carbonyls, and double bonds. Chemical shifts in the NMR spectrum also helped identify its structure.
Glossary definitions and references:
Scientific and Ayurvedic Glossary list for “Terpenoids from cassia siamea”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.
1) Chauhan:
Chauhan refers to Deepa Chauhan, one of the authors who contributed to the research published in the World Journal of Pharmaceutical Research. Her work focuses on isolating and characterizing new triterpenoids from Cassia siamea, highlighting her scientific expertise and contribution to the field of natural products chemistry.
2) Sugar:
Sugar in this context refers to the glucopyranoside moieties found within the triterpenoids isolated from Cassia siamea. These sugar components are significant because they play a crucial role in the bioactivity of glycosides, impacting the solubility, absorption, and pharmacological properties of the compounds being studied.
3) Table:
The term Table here pertains to the tabular representation of the 13C NMR spectral data of compounds 1 and 2, which is crucial for the identification and elucidation of their structural characteristics. Such tables provide a concise format for comparing spectral data to validate compound structures in research.
4) Alder:
Alder in this context likely refers to the Diels-Alder reaction, a well-known cycloaddition reaction in organic chemistry. This reaction is essential to the mass spectral analysis discussed in the text, highlighting its role in determining the fragmentation patterns of the terpenoid compounds analyzed in the study.
5) Flavonoid:
Flavonoids are a class of plant secondary metabolites known for their antioxidant and anti-inflammatory properties. Although not the primary focus of the study, the mention of flavonoids in connection with Cassia siamea indicates the plant's diverse bioactive components, suggesting its potential therapeutic uses and importance in herbal medicine.
6) Disease:
Disease refers to health conditions that Cassia siamea has shown potential benefits against, as mentioned in the text. The plant has demonstrated activities against various ailments, including diabetes and infections, indicating its importance in traditional and modern medicine for treating health issues and its relevance in phytochemical research.
7) Family:
In this context, Family refers to the Fabaceae family, to which Cassia siamea belongs. This classification is significant as it relates to the botanical characteristics shared among plants within this family, including leguminous features, and underscores the plant's scientific categorization and its ecological importance within its group.
8) India:
India indicates the geographical region where Cassia siamea is found abundantly. Its significance lies in the plant's ethnopharmacological use in Indian traditional medicine, making it a relevant subject of study for researchers interested in natural remedies and the exploration of indigenous plants for therapeutic purposes.
9) Tree:
Tree refers to the mature stature of Cassia siamea, emphasizing its classification as a large perennial plant. The physical characteristics of the tree—such as its size and flowering habits—are relevant for ecological studies and potential uses in agroforestry, landscaping, and traditional medicine derived from its various parts.
Other Science Concepts:
Discover the significance of concepts within the article: ‘Terpenoids from cassia siamea’. Further sources in the context of Science might help you critically compare this page with similair documents:
Antioxidant activity, Antimicrobial activities, Anti-inflammatory activities, Analgesic activity, Hydroxyl group, Antioxidant activities, Analgesic activities, Diuretic activity, Antipyretic activity, Bioactive compound, Alkaloids and flavonoids, Antipyretic activities, Antidiabetic activities, Stem Bark, Anticancer activities, Antimalarial activities, Diuretic activities, Triterpenoid.