Antimicrobial and hematological effects of Curcuma longa extracts.

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Journal name: World Journal of Pharmaceutical Research
Original article title: Comparative antimicrobial and hematological activity of different leaves and rhizome extract of curcuma longa
The WJPR includes peer-reviewed publications such as scientific research papers, reports, review articles, company news, thesis reports and case studies in areas of Biology, Pharmaceutical industries and Chemical technology while incorporating ancient fields of knowledge such combining Ayurveda with scientific data.
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Author:

Dr. Giri Akhand Rachana, Akhand Pooja and Dr. Pathak A.K


World Journal of Pharmaceutical Research:

(An ISO 9001:2015 Certified International Journal)

Full text available for: Comparative antimicrobial and hematological activity of different leaves and rhizome extract of curcuma longa

Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research

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Summary of article contents:

Introduction

The article presents a study focused on the antibacterial and hematological properties of different extracts from the leaves and rhizomes of Curcuma longa, commonly known as turmeric. Utilizing 95% ethanolic and aqueous extracts, the research examines their effectiveness against various Gram-negative and Gram-positive bacterial strains through the disc diffusion method. This study responds to the growing challenge of antibiotic resistance and highlights the potential of natural products in therapeutic applications.

Antibacterial Activity of Curcuma longa Extracts

The antibacterial activity of Curcuma longa was assessed using 95% ethanolic leaf extract, aqueous leaf extract, and 95% ethanolic rhizome extract against ten bacterial strains, comprising equal numbers of Gram-negative and Gram-positive varieties. The findings reveal that the 95% ethanolic leaf extract demonstrated significant inhibitory effects on several Gram-negative bacteria, including Enterobacter aerogenes and Streptobacillus monaliformis, exhibiting a greater effect compared to rhizome extracts. Conversely, the aqueous leaf extract showed minimal antimicrobial activity against both bacterial categories.

Hematological Impact of Turmeric Extracts

The research explored the hematological effects of turmeric extracts on rats before and after immunization with sheep red blood cells (SRBC). The results indicated that following treatment with the 95% ethanolic leaf extract, there was a notable increase in white blood cell (WBC) counts, suggesting an immunostimulant effect. Over the course of the immunization days, various hematological parameters showed changes, including increases in lymphocytes and overall leukocyte counts, indicating a potential enhancement of the immune response via turmeric leaf extracts.

Comparative Analysis of Efficacy

The study emphasizes the comparative efficacy of the turmeric extracts against standard antibiotics such as Cefotaxime and Amikacin. While those antibiotics demonstrated consistent antibacterial activity across all tested strains, the ethanolic leaf extract proved to be a promising alternative, particularly against specific Gram-negative and a few Gram-positive strains. The contrasting effects of the aqueous extract, which showed negligible antibacterial potential, underlines the importance of the extraction method and solvent choice in harnessing active constituents from plants.

Conclusion

This research highlights the potential utility of Curcuma longa leaf and rhizome extracts as alternative antimicrobial agents in the fight against antibiotic-resistant bacteria. The significant antibacterial activity observed, particularly from the ethanolic leaf extract, alongside the potential immunostimulant effects, bolsters the case for further exploration of turmeric's medicinal properties. Overall, this work contributes to a broader understanding of natural products in pharmacotherapy and the importance of developing new therapeutic strategies to combat microbial infections effectively.

FAQ section (important questions/answers):

What was the focus of the study on Curcuma longa?

The study compared the antibacterial and hematological activity of different extracts of Curcuma longa leaves and rhizomes, examining their effectiveness against various Gram-positive and Gram-negative bacterial strains.

Which extract showed better antibacterial activity against Gram-negative bacteria?

The 95% ethanolic leaf extract demonstrated better antibacterial activity compared to the rhizome extract, particularly against Enterobacter aerogenes and Streptobacillus monaliformus.

How was the antibacterial activity assessed in the study?

Antibacterial activity was measured using the disc diffusion method, recording the zone of inhibition around the discs after incubating with various bacterial strains.

What were the results of the aqueous leaf extract?

The aqueous leaf extract exhibited no significant antibacterial activity against both Gram-positive and Gram-negative bacterial strains tested in the study.

What was the yield percentage of different extracts?

The yield percentages of the extracts were 14.76% for 95% ethanolic leaf, 41.92% for 95% ethanolic rhizome, and 13.6% for chlorinated water maceration.

Did any extract show immunomodulatory effects?

Yes, the study indicated that various extracts of Curcuma longa had immunostimulant effects, enhancing certain hematological parameters after treatment and immunization.

Glossary definitions and references:

Scientific and Ayurvedic Glossary list for “Antimicrobial and hematological effects of Curcuma longa extracts.”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.

1) Activity:
In the context of this study, 'activity' refers to the biological effects observed when testing the antimicrobial properties of Curcuma longa extracts. The focus is on measuring the efficiency of these extracts against different bacterial strains, providing insights into their potential as natural antibacterial agents.

2) Antibiotic (Antibacterial):
Referring to substances with the ability to inhibit the growth of bacteria, 'anti-bacterial' highlights the extracts' effectiveness against both Gram-positive and Gram-negative strains. This study assesses the comparative efficacy of Curcuma longa extracts against these bacteria, demonstrating its relevance in natural medicine.

3) Curcuma longa:
Curcuma longa, commonly known as turmeric, is a flowering plant of the ginger family widely recognized for its culinary and medicinal applications. The phytochemicals derived from its rhizomes exhibit various health benefits, including anti-inflammatory, antioxidant, and antibacterial properties, which are explored in this research.

4) Turmeric:
Turmeric, derived from the rhizome of Curcuma longa, is renowned for its golden pigment and medicinal qualities. Highlighted throughout the study, turmeric is utilized not only as a spice but also for its medicinal properties against infections and various diseases, thus emphasizing its importance in traditional medicine.

5) Drug:
A drug is any substance used to prevent, treat, or diagnose disease. In this study, extracts from Curcuma longa are analyzed for their therapeutic potential, specifically their antibacterial and immunomodulatory effects, leading to the identification of natural compounds that may serve as new drug candidates.

6) Study (Studying):
This study aims to evaluate the antibacterial and hematological effects of Curcuma longa extracts. It systematically investigates the extracts' efficacy against various bacterial strains and their potential as medicinal interventions, contributing valuable knowledge to both pharmacology and traditional medicine.

7) Aureus:
Staphylococcus aureus is a common bacterium that can cause serious infections in humans. This study evaluates the antibacterial activity of Curcuma longa extracts against this pathogen, highlighting the importance of finding effective treatments in light of antibiotic resistance.

8) Medicine:
Medicine encompasses various discovered and employed methods for treating and preventing diseases. The study of Curcuma longa reflects traditional practices in Ayurveda, focusing on how herbal extracts can complement modern treatments and the importance of botanical resources in health care.

9) Table:
In the context of this study, 'table' refers to organized data presentation of results, enabling clear comparison of the antibacterial activity of various extracts. Tables summarize findings and highlight differences in efficacy between the tested extracts and antibiotics against chosen bacterial strains.

10) Blood:
Blood is a crucial biological fluid transporting oxygen, nutrients, and immune components within organisms. This study measures the hematological effects of Curcuma longa extracts and examines how these extracts impact immune response and blood components, specifically after immunization in animal models.

11) Substance:
A substance refers to any specific material examined for its properties or effects. In this study, Curcuma longa extracts are treated as substances that possess systematic biological activity, and their antibacterial properties are evaluated through rigorous testing methods to determine their safety and efficacy.

12) Powder:
Powder refers to the fine particulate matter produced by grinding the dried leaves or rhizomes of Curcuma longa. The powdered form is essential for extraction processes and is used in this study to prepare various extracts for antibacterial testing, contributing to the overall evaluation of their efficacy.

13) Water:
Water serves as a solvent in the extraction of phytochemicals from Curcuma longa leaves, particularly in preparing aqueous extracts. This extraction method facilitates the dissolution of bioactive compounds, allowing for the subsequent evaluation of their biological properties and potential therapeutic applications.

14) Pharmacological:
Pharmacological research encompasses the study of how substances interact with biological systems. This study assesses the pharmacological activity of Curcuma longa extracts, examining their antibacterial and immunomodulatory effects, thus contributing valuable insights into their potential as therapeutic agents.

15) Container:
The term 'container' refers to the glass or similar vessel used for storing extracts during preparation. Proper containment is essential to maintain the stability of the extracted compounds and ensure no contamination occurs, thus preserving the integrity of the results obtained from the study.

16) Disease:
Disease denotes a pathological condition disrupting normal bodily function. The study investigates how the antibacterial properties of Curcuma longa extracts could provide alternatives for treating infections caused by resistant bacteria, bridging traditional knowledge with modern medical requirements.

17) Cancer:
Cancer represents a range of diseases characterized by uncontrolled cell growth. Curcuma longa is researched for its beneficial effects on cancer prevention and treatment, including its antitumor properties, which are relevant to the exploration of natural compounds as potential adjunctive therapies.

18) Animal:
Animal models are essential in biomedical research for testing the safety and efficacy of substances. This study utilizes animal subjects to assess the immunological and hematological responses to Curcuma longa extracts, providing insights that are often translatable to human health outcomes.

19) India:
India, the country of origin for Curcuma longa, has a rich tradition of employing herbal medicine within its Ayurvedic practices. This context is vital for understanding the cultural significance and historical usage of turmeric as a remedy in various health conditions throughout the region.

20) Glass:
Glass refers to the material used to create containers and apparatus in laboratory settings. The importance of glass is significant in extraction processes, as it avoids chemical reaction with extracts, ensuring the accuracy and reliability of results in this study.

21) Giri:
Giri is likely one of the researchers involved in this study, Dr. Giri Akhand Rachana, whose contributions to the field of pharmacy and medicinal plant research are detailed within this publication. This highlights the collaborative nature of academic research and associated expertise.

22) Inflammation:
Inflammation is a biological response to harmful stimuli like pathogens. Some extracts from Curcuma longa are known for their anti-inflammatory effects, which could provide insights not only into antibacterial activity but also into reducing inflammation, thus offering a dual therapeutic approach.

23) Observation:
Observation in this context pertains to the systematic tracking of changes during experimental interventions, including antibacterial effects and hematological parameters. Accurate observations are fundamental to validating results, ensuring that conclusions drawn from the study are scientifically supported.

24) Discussion:
The discussion section of research presents interpretations of results, addressing their significance and implications within broader scientific contexts. In this study, discussions any advancements on traditional uses of Curcuma longa and explored its viability as an alternative antimicrobial treatment.

25) Tamilnadu (Tamil-nadu):
Tamil Nadu, a state in India, is one of the regions where Curcuma longa is cultivated extensively. Its geographical significance and historical usage in local medicine further amplify the role of turmeric as a staple in various health practices and culinary traditions.

26) Harvesting (Harvest):
Harvesting refers to the process of gathering mature plants for use. In the context of Curcuma longa, effective harvesting techniques ensure quality yields of rhizomes and leaves, which are essential for subsequent extraction processes studied for various therapeutic benefits.

27) Karnataka:
Karnataka, another Indian state known for turmeric cultivation, plays an essential role in the agricultural and economic aspects of this spice. Highlighting diverse sourcing regions, the study emphasizes the importance of geographic diversity in obtaining quality Curcuma longa for research.

28) Samjivani (Sanjivani):
Sanjivani likely refers to Sanjivani Ayurveda, a source for collecting Curcuma longa plant material in the study. This points to the use of authenticated sources in research, ensuring that the herbal material meets quality standards critical for scientific investigations.

29) Evolution:
Evolution denotes the gradual development of antibiotic resistance in microbial populations. This study emphasizes the need for alternative treatments as traditional antibiotics lose effectiveness; thus, examining Curcuma longa extracts could provide promising options for combating resistant strains.

30) Colouring (Coloring):
Colouring refers to the use of Curcuma longa in the food industry as a natural dye due to its vibrant yellow pigment, curcumin. Such applications highlight the multifaceted uses of the plant, enhancing its relevance in both culinary and medicinal domains.

31) Composite:
Composite signifies the combination of various constituents in Curcuma longa that contribute to its medicinal properties. Understanding the complex interactions of these components can aid in developing standardized extracts for consistent therapeutic applications.

32) Ayurveda (Ayus-veda):
Ayurveda is the traditional Indian system of medicine that utilizes natural remedies and holistic healing practices. The study underscores the significance of Curcuma longa in Ayurveda, showcasing its long-established role in treating ailments and promoting health through herbal preparations.

33) Epidemic:
An epidemic refers to widespread occurrence of infectious diseases. The rising rates of antibiotic resistance contribute to the importance of finding alternative treatments like the ones derived from Curcuma longa, which may aid in managing infectious diseases more effectively.

34) Chandra:
Chandra, possibly Dr. Chandra R., is another contributing author in the research. The collaboration highlights the joint effort and expertise involved in carrying out this study, exemplifying the collective knowledge necessary for exploring medicinal plant properties.

35) Cutting:
Cutting pertains to the preparation of plant material for experimentation, such as chopping leaves. Proper cutting techniques affect the extraction efficiency of bioactive compounds and influence the outcomes observed in the study, emphasizing the methodological rigor required in research.

36) Leprosy:
Leprosy is a chronic infectious disease historically documented in Ayurvedic texts. Curcuma longa's antibacterial properties are significant in potential treatments for such conditions, reaffirming its relevance in traditional healing practices while exploring its modern applications in combating infections.

37) Surface:
The surface represents the area of agar plates where extracts and antibiotics are applied during testing. This is crucial for determining the zone of inhibition and evaluating the efficacy of each extract against specific bacterial strains.

38) Andhra (Amdhra):
Andhra Pradesh is another Indian state that contributes to the cultivation of turmeric. This highlights the geographic scope of turmeric farming in India, leading to increased biodiversity and sources for research into Curcuma longa’s pharmacological applications.

39) Bengal (Bemgal):
Bengal, specifically West Bengal, is known for its extensive cultivation of Curcuma longa. Highlighting the historical and contemporary relevance of this region underscores the plant's cultural significance and the existing knowledge of its medicinal use.

40) Family:
In botanical classification, 'family' pertains to a group of related plants. Curcuma longa belongs to the Zingiberaceae family, which identifies its taxonomic relations and influences the understanding of potential similarities in phytochemical activities and therapeutic benefits among family members.

41) Reason:
Reason reflects the justification for conducting the study, primarily the rise of antibiotic resistance and the need for effective natural alternatives. This highlights the significance of examining Curcuma longa extracts and their potential applications in combating resistant bacterial strains.

42) Medium:
Medium refers to the growth environment used for bacterial cultures, such as Mueller-Hinton agar in this study. The right medium is critical for accurate testing of antibacterial activity, influencing the viability of bacteria and reliability of results obtained.

43) Indian:
Indian refers to the context of Ayurveda and traditional medicine practiced in India, where Curcuma longa is indigenous. The cultural background enriches the understanding of turmeric's historical significance and its practical applications in contemporary health practices.

44) Manure:
Manure highlights the agricultural aspect of using turmeric leaves as green manure, suggesting that even waste products from turmeric cultivation can be utilized effectively. This points to the ecological importance of plant resources within sustainable agricultural practices.

45) Lanka (Lamka, Lànkà):
Lanka, referring to Sri Lanka, is known for high-quality turmeric production. The inclusion of Sri Lanka in discussions of turmeric cultivation emphasizes regional production importance and the spice's economic significance within international markets.

46) Bihar:
Bihar is another state in India where Curcuma longa is cultivated. This indicates the geographical diversity in turmeric production within India, providing various sources for research and highlighting the implications for local agricultural practices and cultural significance.

47) Pooja:
Pooja is another author involved in this research. Collaborative efforts are crucial for comprehensive studies, representing the combined expertise and contributions of multiple researchers in understanding the pharmacological aspects of Curcuma longa.

48) House:
House may refer conceptually to sustained research or a laboratory setting for conducting experiments. Adequate facilities for conducting the study influence the quality of research processes and outcomes derived from the exploration of Curcuma longa extracts.

49) Tamil:
Tamil refers to language and culture prevalent in Tamil Nadu, India. This adds contextual depth when discussing the regional significance of turmeric in the local traditional medicine and its prominence in historical and contemporary practices.

50) Cina:
China is known for its extensive history with herbal medicine, including turmeric. Highlighting its role signifies the widespread cultural recognition and utilization of Curcuma longa across different regions, strengthening its relevance in comprehensive medicinal studies.

51) Java:
Java, an island in Indonesia, is another region associated with turmeric cultivation. The mention of Java emphasizes the global cultivation of Curcuma longa, stressing the geographic diversity in sourcing and applications of this valuable medicinal plant.

52) Nadu:
Nadu, as in Tamil Nadu, points to an important Indian state in the cultivation of turmeric. It underlines regional agricultural significance and the longstanding tradition of using turmeric in diverse health practices within the state.

53) Food:
Food underscores the importance of turmeric as a common spice used in culinary practices. This highlights the plant's multifaceted role, integrating culinary enjoyment with medicinal benefits, thus bridging nutrition and health in dietary contexts.

54) Crop:
Crop underscores the agricultural context of turmeric cultivation, indicating its economic importance. As a crop, Curcuma longa greatly contributes to the livelihoods of farmers and the economy, particularly in traditional farming regions where it is a staple specimen.

55) Post:
Post refers to the time following activities like harvesting or processing. For example, post-harvesting practices are crucial for ensuring the quality of turmeric and maximizing the efficacy of extracts used in research studies for antibacterial and health-promoting properties.

Other Science Concepts:

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Discover the significance of concepts within the article: ‘Antimicrobial and hematological effects of Curcuma longa extracts.’. Further sources in the context of Science might help you critically compare this page with similair documents:

Curcuma longa, Medicinal plant, Antibiotic, Treatment, Adverse effect, Synthetic antibiotic, Medicinal use, Antibacterial activity, Haemoglobin content, Disc diffusion method, Zone of inhibition, Immune response, Sheep RBC, Gram positive bacteria, Gram negative bacteria, Hygroscopic nature, RBC count, Bacterial resistance, Amino acid, Packed cell volume, Biological activities, WBC count, Anti bacterial activity, Phytochemical properties, Gram positive, Gram negative, Bacterial strain, Total leucocyte count, Delayed type hypersensitivity, Hematological Profile, Pharmacological effect, Immunostimulant activity, Laboratory techniques, Gram Negative Bacterial Strain, Extraction Protocol, Blood sample, Haematological profile, Amikacin, Active chemical constituent, Leukocytes, Haematological parameter, Indigenous herbal medicine.

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