Phytochemical and pharmacological screening of elaeocarpus tuburculatus

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Journal name: World Journal of Pharmaceutical Research
Original article title: Phytochemical and pharmacological screening of elaeocarpus tuburculatus
The WJPR includes peer-reviewed publications such as scientific research papers, reports, review articles, company news, thesis reports and case studies in areas of Biology, Pharmaceutical industries and Chemical technology while incorporating ancient fields of knowledge such combining Ayurveda with scientific data.
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Author:

Raju HV, Ganapathy S, Prasanna S Sutar, Kishori P Sutar


World Journal of Pharmaceutical Research:

(An ISO 9001:2015 Certified International Journal)

Full text available for: Phytochemical and pharmacological screening of elaeocarpus tuburculatus

Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research

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Summary of article contents:

Introduction

The study focuses on the phytochemical and pharmacological properties of Elaeocarpus tuburculatus, a medicinal plant used traditionally for various ailments. Given that approximately 60% of the world's population relies on traditional medicine for healthcare, the investigation of plants like Elaeocarpus tuburculatus is increasingly relevant. This research aims to provide scientific evidence regarding the anti-inflammatory and antifungal activities found in the chloroform and methanolic extracts of the bark of this plant, acknowledging its potential therapeutic benefits amidst the growing interest in natural remedies.

Phytochemical Profile and Extract Composition

The phytochemical examination of Elaeocarpus tuburculatus involved successively extracting the bark with chloroform and methanol. Various chemical constituents such as steroids, alkaloids, tannins, flavonoids, carbohydrates, and glycosides were identified in these extracts. Through techniques like thin layer chromatography and column chromatography, several active compounds were isolated and characterized, including β-Sitosterol, Cucurbitacin D, Cucurbitacin F, Kaempferol, Quercetin, Rutin, and Scopoletin. Each compound exhibited distinct properties and contributed to the overall bioactivity of the extracts, highlighting the importance of these phytochemicals in medicinal applications.

Anti-Inflammatory Activity

The anti-inflammatory effects of the extracts were evaluated using a carrageenan-induced paw edema model in Wistar albino rats. The experiments demonstrated that both the chloroform and methanolic extracts possess anti-inflammatory properties, with the methanolic extract showing more significant effects compared to the chloroform extract. The results indicated a dose-dependent relationship; higher doses led to greater inhibition of edema, establishing the extracts' potential as natural anti-inflammatory agents in comparison to ibuprofen, a standard anti-inflammatory drug.

Comparative Efficacy of Extracts

Results showed that the methanolic extract of Elaeocarpus tuburculatus was more effective than the chloroform extract in reducing paw edema induced by carrageenan. The study documented that the percentage inhibition of edema significantly increased with the dose of the methanolic extract. Specifically, at the highest tested doses, the methanolic extract matched the effectiveness of the reference standard ibuprofen, suggesting that the bioactive compounds present in the extracts may offer similar therapeutic benefits as synthetic drugs.

Conclusion

In conclusion, the research provides valuable insights into the pharmacological properties of Elaeocarpus tuburculatus, particularly its anti-inflammatory effects. Both chloroform and methanolic extracts demonstrated the potential for medicinal use, with the methanolic extract showing superior efficacy. This study supports the notion that traditional medicinal plants could serve as viable alternatives or complements to modern pharmaceuticals, reinforcing the need for further exploration and validation of their therapeutic applications. The findings encourage continued research into the potential benefits that natural compounds can offer in treating inflammation-related conditions.

FAQ section (important questions/answers):

What are the main findings regarding Elaeocarpus tuburculatus extracts?

The investigation found that chloroform and methanolic extracts of Elaeocarpus tuburculatus bark possess significant anti-inflammatory and antifungal activities.

What types of extracts were used in the study?

The study utilized chloroform and methanolic extracts of Elaeocarpus tuburculatus bark, prepared from air-dried and powdered bark.

What method was used to test anti-inflammatory activity?

Anti-inflammatory activity was assessed using the carrageenan-induced paw edema method in Wistar albino rats.

What were the dosages administered to the rats in the study?

Rats were administered extracts at dosages of 100, 300, and 500 mg/kg body weight, with Ibuprofen as a control.

What chemical constituents were identified in the extracts?

Chemical analysis revealed the presence of steroids, alkaloids, flavonoids, tannins, and glycosides in both chloroform and methanolic extracts.

What were the significant effects of the methanolic extracts?

The methanolic extracts showed significant anti-inflammatory activity, reducing paw edema in a dose-dependent manner compared to control.

Glossary definitions and references:

Scientific and Ayurvedic Glossary list for “Phytochemical and pharmacological screening of elaeocarpus tuburculatus”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.

1) Substance:
Substances refer to the chemical materials derived from plants or minerals utilized in pharmaceutical formulations. In the research context, the substance includes the extracts from the bark of Elaeocarpus tuburculatus, which were analyzed for their pharmacological properties, such as anti-inflammatory and antifungal activities, showcasing the significance of traditional remedies.

2) Edema (Oedema):
Edema, synonymous with oedema, refers to the swelling caused by fluid accumulation. The study specifically investigates the effects of Elaeocarpus tuburculatus extracts on edema induced in rats, using these metrics as key indicators for assessing the extracts' anti-inflammatory efficacy and therapeutic potential.

3) Activity:
Activity in this research context pertains to the pharmacological effects exhibited by the extracts of Elaeocarpus tuburculatus. The study aimed to demonstrate the anti-inflammatory and antifungal activity through laboratory tests, comparing the extracts' performance against established drugs like Ibuprofen to validate their potential therapeutic benefits.

4) Medicine:
Medicine encompasses the science and practice of diagnosing, treating, and preventing illnesses. In the study, traditional medicine approaches were explored by examining natural extracts from the Elaeocarpus tuburculatus plant, showcasing its potential role in developing alternative therapeutic options within both traditional and modern medicinal practices.

5) Karnataka:
Karnataka is a state in India, known for its rich biodiversity and vast resources of medicinal plants. The study was conducted in Karnataka, highlighting the region's contribution to traditional medicine by investigating local species such as Elaeocarpus tuburculatus, thereby reinforcing the importance of regional botany in pharmacological research.

6) India:
India has a profound historical and cultural heritage of traditional medicine, utilizing various plant species for health care. This research emphasizes India’s significance as a source of medicinal herbs and contributes to global knowledge on the pharmacological potentials of indigenous flora, augmenting both local and international medicinal practices.

7) Drug:
A drug is a substance used in the diagnosis, treatment, or prevention of disease. The research involves evaluating the extracts of Elaeocarpus tuburculatus as potential natural drugs, assessing their therapeutic activities, and comparing them to synthetic drugs like Ibuprofen for effective management of inflammation and other ailments.

8) Science (Scientific):
Scientific pertains to a methodological approach to understanding phenomena through observation and experimentation. The study employs scientific principles in assessing the pharmacological benefits of Elaeocarpus tuburculatus, linking traditional remedies with empirical evidence, exemplifying the transition toward evidence-based natural therapies.

9) Table:
Tables in research articles serve to organize and present data clearly and concisely, facilitating better comprehension and comparison. In this study, tables summarize the anti-inflammatory effects of different extracts, providing critical insights into the experimental results and allowing for easy interpretation of the findings.

10) Inflammation:
Inflammation is a biological response to harmful stimuli, and assessing its reduction is vital in evaluating therapeutic efficacy. The study focuses on inflammation induced in rats using carrageenan to evaluate the anti-inflammatory properties of Elaeocarpus tuburculatus extracts, aiming to find effective treatments for inflammatory conditions.

11) Animal:
Animal models, such as Wistar albino rats used in this research, are crucial for studying the biological effects of substances in a controlled environment. The study's use of animal testing allows researchers to explore the pharmacological properties of plant extracts and their potential therapeutic applications before proceeding to human studies.

12) Powder:
Powder refers to the physical state of the plant material after processing, impacting the extraction and analysis of bioactive compounds. In this investigation, the bark of Elaeocarpus tuburculatus was powdered to facilitate the extraction process, ensuring effective isolation and concentration of phytochemicals for subsequent pharmacological evaluation.

13) Study (Studying):
A study involves a detailed investigation aimed at understanding a specific area of research. This specific study examines the phytochemical and pharmacological properties of Elaeocarpus tuburculatus extracts, employing various methods to scientifically validate traditional medicinal claims, contributing to the body of knowledge in pharmacognosy.

14) Pharmacological:
Pharmacological refers to the effects and mechanisms of drug action within biological systems. The study investigates the pharmacological properties of extracts from Elaeocarpus tuburculatus, highlighting their potential therapeutic benefits as anti-inflammatory agents, thus bridging the gap between traditional remedies and evidence-based medicine.

15) Phytochemical:
Phytochemical pertains to the chemical compounds produced by plants, often related to their health benefits. The study emphasizes the identification of various phytochemicals in the extracts of Elaeocarpus tuburculatus, aiming to correlate these compounds with observed pharmacological activities, enriching the understanding of plant-based therapies.

16) Developing:
Developing in this context refers to the process of creating new pharmaceutical therapies. The study contributes to developing alternative treatments by investigating indigenous plants like Elaeocarpus tuburculatus, addressing the growing interest in natural remedies as safer, effective options in modern healthcare systems.

17) Flavonoid:
Flavonoids are a diverse group of phytonutrients found in many fruits and vegetables, known for their antioxidant and anti-inflammatory properties. The research explores the presence of flavonoids in the extracts of Elaeocarpus tuburculatus, emphasizing their potential role in therapeutic applications and health promotion within traditional medicine systems.

18) Prasanna:
Prasanna S Sutar is one of the authors of the study, contributing to the research within the field of pharmacognosy. His work involves investigating the medicinal properties of local plant species, reflecting the essential role of researchers in expanding the scientific understanding of traditional medicinal practices.

19) Disease:
Disease refers to any abnormal condition affecting the body which requires medical intervention. The study targets diseases associated with inflammation and aims to explore traditional plant extracts, such as that of Elaeocarpus tuburculatus, for their potential in treating various illnesses through their pharmacological benefits.

20) Species:
Species denotes a group of related organisms that share common characteristics. In this study, the species Elaeocarpus tuburculatus is examined for its medicinal properties, emphasizing the significance of biodiversity in providing potential natural remedies and the relevance of specific species in traditional medicine.

21) Andhra (Amdhra):
Andhra Pradesh is a state in India known for its rich agricultural culture and diverse medicinal plants. The mention of Andhra in the study underscores the geographical context in which traditional plant species thrive, contributing to broader discussions about the uses of local flora in health and healing practices.

22) Mandal:
Mandal refers to an administrative region in India. The inclusion of Mandal in the research context likely indicates the area where Elaeocarpus tuburculatus is found, highlighting the importance of local knowledge and regional biodiversity in sourcing traditional medicinal plants for pharmacological study.

23) Hind:
Hind often relates to the cultural and historical context of India. In the research landscape, understanding the traditional uses of medicinal plants like Elaeocarpus tuburculatus within the historical Hind context is pivotal in recognizing the deep-rooted practices of herbal medicine and the transition to contemporary scientific methods.

24) Civilization:
Civilization pertains to advanced human societies showcasing cultural, social, and technological development. The study of traditional medicine reflects India's ancient civilizations, where herbal knowledge was crucial for health. Insights from such civilizations enrich current pharmacological research, allowing for the validation of ancient practices using modern scientific techniques.

25) Discussion:
Discussion in scientific research provides a platform to interpret results, relate findings to existing knowledge, and suggest implications. In this study, the discussion aims to analyze the effectiveness of Elaeocarpus tuburculatus extracts, contextualizing their anti-inflammatory properties within broader pharmacological literature to support traditional claims.

26) Heating:
Heating refers to a process that can influence the chemical properties of substances. In the study, heating may be related to the extraction methods used for isolating bioactive compounds from Elaeocarpus tuburculatus, emphasizing the importance of proper technique to preserve the efficacy of obtained extracts.

27) Mineral:
Minerals are naturally occurring inorganic substances essential to human health. While the primary focus of the study is on plant extracts, understanding the role of minerals in conjunction with herbal treatments may provide a holistic view of therapeutic options within natural medicine, emphasizing comprehensive approaches to health.

28) Forest:
Forest ecosystems are vital for biodiversity and contain numerous medicinal plants. The study's reference to regions like Karnataka, where Elaeocarpus tuburculatus grows, underscores the importance of forests as repositories of traditional medicinal knowledge, supporting conservation efforts and the sustainable use of plant resources.

29) Cancer:
Cancer is a major health concern characterized by the uncontrolled growth of cells. Though not the primary focus of the study, exploring the medicinal properties of plants like Elaeocarpus tuburculatus may contribute to future cancer treatment research, highlighting the need for diverse sources for potential therapies.

30) Indian:
Indian refers to the cultural and geographical context of India known for its ancient traditions of herbal medicine. The research emphasizes the significance of Indian flora, such as Elaeocarpus tuburculatus, in traditional healing practices, illustrating the importance of indigenous plant knowledge in contemporary pharmacological studies.

31) Water:
Water is essential for life and is often used as a solvent in extractions. In the study, water's role could pertain to its use in preparing solutions for pharmacological testing or its influence on the biological activities observed in the extracts of Elaeocarpus tuburculatus.

32) Rani:
Rani Chennamma College of Pharmacy, associated with one of the authors, signifies collaboration within academic institutions for pharmaceutical research. The involvement of educational entities like Rani Chennamma College demonstrates the connection between traditional knowledge and modern educational frameworks advancing pharmacognosy.

33) Genu:
In a research context, Genu may refer to specific anatomical structures or components pertinent to the study's methodology. If related to animal models, understanding anatomical variations can be critical for interpreting biological responses, particularly in context to inflammation measured through paw oedema in this study.

34) Food:
Food encompasses substances consumed for nutritional support, often containing bioactive compounds. The study indirectly relates food to the holistic health perspective where plants like Elaeocarpus tuburculatus not only serve medicinal purposes but may also provide dietary benefits, aligning nutrition with traditional therapeutic practices.

35) Tree:
Tree symbolizes the source of medicinal products, in this case, Elaeocarpus tuburculatus. Distinct species of trees contribute significantly to the pharmacopoeias of traditional medicine. Understanding diverse tree species is crucial for harnessing their benefits in modern medicine, highlighting the link between environment, biodiversity, and health.

Other Science Concepts:

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Discover the significance of concepts within the article: ‘Phytochemical and pharmacological screening of elaeocarpus tuburculatus’. Further sources in the context of Science might help you critically compare this page with similair documents:

Research work, Traditional medicine, Statistical analysis, Anti-inflammatory activity, Pharmacological activities, Thin Layer Chromatography, One-way ANOVA, Rutin, Quercetin, Analytical grade, Professor and Head, Chloroform extract, Kaempferol, Methanolic extract, Column chromatography, Melting point, Carrageenan, Ferric chloride, Carrageenan induced paw oedema, Ibuprofen, Chemical constituent, Department of Pharmacognosy, Lead acetate, Phytochemical examination, Reference Standard, Volume displacement method, Color reaction, Co-TLC, Acknowledgment, Wistar albino rat, Percentage inhibition of oedema.

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