Himalayan juniper (Juniperus squamata): Phytochemical and antimicrobial assessment.

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Journal name: World Journal of Pharmaceutical Research
Original article title: Assessment of himalayan juniper, juniperus squamata buch– ham ex d. don for phytochemical screening and antimicrobial potential against some infection causing pathogens
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Author:

S. C. Sati and Parikshit Kumar


World Journal of Pharmaceutical Research:

(An ISO 9001:2015 Certified International Journal)

Full text available for: Assessment of himalayan juniper, juniperus squamata buch– ham ex d. don for phytochemical screening and antimicrobial potential against some infection causing pathogens

Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research

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Summary of article contents:

Introduction

The study published in the World Journal of Pharmaceutical Research focuses on the phytochemical screening and antimicrobial potential of Himalayan juniper, scientifically known as Juniperus squamata Buch.-Ham ex D. Don. Conducted by S. C. Sati and Parikshit Kumar, this research highlights the importance of medicinal plants in drug discovery, particularly in the context of rising antibiotic resistance among pathogens. The investigation sought to explore both the chemical constituents of J. squamata and its efficacy against various pathogenic bacteria, thus contributing valuable insights into its potential as a source of novel antimicrobial agents.

Phytochemical Constituents of Juniperus squamata

The qualitative phytochemical analysis of the leaves revealed a rich diversity of compounds in both ethanol and methanol extracts. A range of phytochemicals such as alkaloids, flavonoids, carbohydrates, glycosides, proteins, saponins, terpenoids, phenols, starch, and resins was found in the extracts, highlighting the complex biochemical nature of this gymnosperm. Notably, the ethanol extract showed the presence of 11 phytochemicals, while the methanol extract contained 13. The absence of phlobatannins and anthraquinones in both extracts further indicates the unique chemical profile of J. squamata leaves.

Antimicrobial Activity Against Pathogens

The antimicrobial activity of the leaf extracts was assessed through a disc-diffusion method against five pathogenic bacteria: Agrobacterium tumefaciens, Bacillus subtilis, Escherichia coli, Erwinia chrysanthemi, and Xanthomonas phaseoli. The ethanol extract demonstrated the highest antibacterial efficacy, with a maximum zone of inhibition measuring 21 mm against E. chrysanthemi. Additionally, the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values were determined, revealing that the ethanol extract had MIC values ranging from 31.25 to 500 μg/ml and MBC from 125 to 500 μg/ml, indicating its potential as a therapeutic agent.

Methodological Rigor

The study employed rigorous methodologies for both phytochemical analysis and antimicrobial testing. The extraction procedure involved soaking the dried leaves in methanol and ethanol, followed by filtering and concentrating the solutions. Phytochemical analyses followed established protocols to ensure reliable results in identifying various bioactive compounds. The antibacterial testing utilized triplicate samples to enhance accuracy, with comparisons made against standard antibiotic controls. This meticulous approach lends credibility to the findings and highlights the scientific basis for exploring medicinal plants as alternatives to conventional antibiotics.

Conclusion

In conclusion, the research underscores the significant antimicrobial potential of Juniperus squamata, revealing its promise as a source of novel antimicrobial agents against common pathogens. The presence of various bioactive phytochemicals supports its traditional uses in herbal medicine. Given the increasing prevalence of antibiotic resistance, the exploration of such natural products is crucial. The findings advocate for further studies to validate the medicinal properties of J. squamata, which could pave the way for its integration into pharmaceutical applications aimed at combating bacterial infections. This pioneering investigation into a high-altitude Himalayan gymnosperm emphasizes the need for continued exploration of indigenous flora to unearth new medicinal compounds.

FAQ section (important questions/answers):

What is the focus of the study on Juniperus squamata?

The study assesses the phytochemical composition and antimicrobial potential of Juniperus squamata against five pathogenic bacteria, using methanol and ethanol extracts to determine antibacterial activity.

How were the antibacterial activities of the extracts measured?

Antibacterial activities were measured using the disc-diffusion method, determining the zone of inhibition against various bacteria cultured on nutrient agar plates.

What phytochemicals were found in Juniperus squamata extracts?

Phytochemicals identified include alkaloids, flavonoids, carbohydrates, glycosides, proteins, saponins, terpenoids, phenols, starch, and resins, with a few varying by solvent used.

Which extract showed higher antibacterial activity?

The ethanol extract demonstrated greater antibacterial activity, especially against Erwinia chrysanthemi, with a maximum zone of inhibition recorded.

What are the Minimum Inhibitory Concentration (MIC) values found?

MIC values for the ethanol extract ranged from 31.25 to 500 µg/ml, indicating its effectiveness against tested pathogenic bacteria.

What is the significance of this study's findings?

The findings highlight Juniperus squamata's potential as a source for antimicrobial agents, supporting its traditional medicinal uses and possibility for leveraging in pharmaceutical applications.

Glossary definitions and references:

Scientific and Ayurvedic Glossary list for “Himalayan juniper (Juniperus squamata): Phytochemical and antimicrobial assessment.”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.

1) Phytochemical:
Phytochemicals are bioactive compounds found in plants that contribute to their medicinal properties. They play significant roles in various health benefits, including anti-inflammatory, antioxidant, and antimicrobial effects. Understanding phytochemicals enhances the exploration and utilization of plants like Juniperus squamata for therapeutic purposes.

2) Activity:
Activity in this context refers to the biological effects exhibited by the extracts of Juniperus squamata, particularly their ability to inhibit the growth of pathogenic bacteria. Evaluating such bioactivity is crucial for determining the potential application of plant extracts in healthcare and pharmaceuticals.

3) Antibiotic (Antibacterial):
Antibiotics are medicines used to treat bacterial infections, but challenges like antibiotic resistance underscore the need for new options. The study's exploration of Juniperus squamata as a potential source of antibacterial compounds is significant in developing alternative treatments for resistant pathogens.

4) Table:
Tables organize and present scientific data clearly, making it easier for readers to understand results. In this research, tables summarize phytochemical evaluations and antibacterial activities, allowing for quick comparisons of the extracts' effectiveness and chemical compositions, thus facilitating data interpretation.

5) Flavonoid:
Flavonoids are a class of phytochemicals known for their antioxidant and anti-inflammatory properties. Within the study, the presence of flavonoids in Juniperus squamata indicates its potential health benefits, including the ability to combat infections and reduce chronic disease risks, making it valuable for further research.

6) Shati (Sati, Sati, Sati, Sati, Sati, Sati, Sati, Sati, Sati):
Sati refers to one of the authors of the study, providing insights into the research on Juniperus squamata. This indicates collaboration and contributions to the scientific knowledge regarding the phytochemical and antibacterial properties of Himalayan plants, enhancing credibility and scientific inquiry.

7) Water:
Water plays a vital role in biological processes, including the extraction of phytochemicals from plant materials. The study used water as part of the extraction process and in the preparation of various tests, highlighting its fundamental importance in both laboratory procedures and nutrient transport in living organisms.

8) Disease:
The term disease indicates a disruption of normal physiological functioning, often requiring intervention. The study focuses on pathogens responsible for common diseases in plants and animals, emphasizing the importance of identifying effective antimicrobial agents from plants to combat infectious diseases and improve health outcomes.

9) India:
India is the geographical context for the research, known for its rich biodiversity and traditional medicinal practices. The study of Juniperus squamata in India emphasizes the potential of local flora in developing herbal medicines and supports the ongoing research for pharmaceutical applications in Ayurveda and modern medicine.

10) Drug:
A drug is a substance that alters physiological functions to treat or prevent diseases. The potential of the phytochemical constituents of Juniperus squamata as new drug candidates for antimicrobial activity reflects the ongoing search for alternative therapies, particularly important in the face of increasing antibiotic resistance.

11) Himalaya (Hima-alaya):
The Himalaya is the region where Juniperus squamata is found, characterized by high-altitude biodiversity and unique ecological conditions. The plants from this region often possess distinct medicinal properties due to their adaptation to harsh environments, making them important subjects in phytochemical research and traditional medicine.

12) Medicine:
Medicine encompasses the field focused on maintaining health and treating diseases through various interventions, including pharmacology and herbal remedies. The study investigates medicinal plants, particularly their extracts, contributing to the body of knowledge needed for developing alternative therapies and enhancing public health.

13) Botany:
Botany is the scientific study of plants, including their structure, properties, and biochemical processes. Understanding botany is crucial for research like this, as it aids in identifying medicinal plants, their phytochemicals, and potential applications in pharmaceuticals, agriculture, and environmental sustainability.

14) Joshi (Josi, Josi):
Joshi, another author of the study, signifies the collaborative effort in researching Juniperus squamata. Academic collaboration heightens the research's depth, ensuring diverse expertise contributes to the thorough investigation of the plant's properties, promoting scientific understanding and potential application in medicine.

15) Knowledge:
Knowledge in this context refers to understanding gained through research about the phytochemical properties and potential medicinal applications of Juniperus squamata. Accumulating such knowledge is vital for informing future studies, thus contributing to advancements in herbal medicine and public health.

16) Heating:
Heating is a technique used in various experiments to extract phytochemicals or initiate chemical reactions. In phytochemical analysis, heating may help release soluble compounds from plant materials, supporting the analysis of active ingredients necessary for assessing the plant's medicinal properties.

17) Species:
Species refers to a group of organisms sharing common characteristics. The study focuses on the species Juniperus squamata, highlighting its unique properties as a well-known medicinal plant in the Himalayas, which contributes to research aimed at discovering new therapeutic agents.

18) Surface:
Surface refers to the layer accessible for interaction, such as when extracts interact with bacteria during antibacterial assays. The surface of agar plates in microbiological tests plays a critical role in determining microbial growth inhibition by allowing extracts to diffuse and affect bacterial activity.

19) Animal:
The term animal encompasses living organisms that may be impacted by pathogens targeted in the study. Understanding how plant extracts can combat infections in animal models emphasizes their potential therapeutic applications and importance in veterinary medicine and zoonotic disease control.

20) Campu:
Campu likely refers to 'campus,' the location related to where the study was conducted, indicating the academic environment of Kumaun University, which adds credibility to the research findings. The university setting fosters collaboration and access to resources critical for scientific inquiry.

21) Kumar:
Kumar, the corresponding author, indicates one of the key contributors to the study. Authorial involvement signifies commitment to the research process and highlights collaboration in scientific exploration, enhancing the credibility and depth of the findings discussed in the paper.

22) Study (Studying):
The study refers to the research project conducted to assess the phytochemical and antibacterial properties of Juniperus squamata. It contributes to the understanding of the plant's medicinal potential and is part of the broader endeavor to discover alternative and effective therapeutic agents.

23) Vata:
Vata is a term from Ayurvedic medicine referring to one of the three doshas, or bodily energies, associated with wind and space. The study highlights the traditional uses of Juniperus squamata in Ayurvedic practices, showcasing its role in addressing various health conditions relevant to local cultures.

24) Food:
Food refers to substances consumed for nutritional value. The significance of plant extracts from Juniperus squamata extends beyond medicinal uses; they also offer dietary benefits that align with traditional practices, supporting the importance of understanding their nutritional and health-promoting properties.

25) Rich (Rch):
Rich, in this context, describes the biodiversity of medicinal plants and their phytochemical compounds. The richness of such resources is key for developing new pharmaceutical products, emphasizing the need for comprehensive studies to harness the full potential of plants like Juniperus squamata.

26) Hand:
Hand could refer to manual techniques used in preparing extracts or conducting experiments. In scientific research, hands-on methods are crucial for ensuring accurate data collection, influencing the reliability and validity of results, particularly when examining the properties of medicinal plants.

27) Animal disease:
Animal disease encompasses health conditions affecting non-human species, which may be addressed by the antibacterial properties of plant extracts. The findings suggest that compounds from Juniperus squamata could provide new solutions for managing infections in animals, benefiting veterinary medicine.

28) Human body:
Human body pertains to the biological organism that may benefit from the medicinal properties of Juniperus squamata. The implications of the study highlight the need for novel therapeutic agents to combat infections and promote health, addressing concerns linked to human health challenges.

29) New Delhi:
New Delhi, as the capital of India, is significant for fostering scientific research and policy development. It is not only a hub for medicinal plant research but also plays a pivotal role in shaping health initiatives aimed at leveraging native flora for therapeutic benefits.

30) Pharmacological:
Pharmacological pertains to the study of drugs and their effects on biological systems. Understanding the pharmacological potential of plant extracts like those from Juniperus squamata is essential for exploring their therapeutic applications and assessing their safety and efficacy in medical treatments.

31) Tuberculosis:
Tuberculosis is a serious infectious disease caused by bacteria, emphasizing the need for effective treatments. The study's context addresses the search for antimicrobial properties in plant extracts, highlighting the potential of Juniperus squamata as an alternative option in combating resistant infections, including tuberculosis.

32) Observation:
Observation in scientific research refers to the detailed assessment of experiments and results. Accurate observation is critical to interpreting findings regarding the effects of Juniperus squamata extracts and their antibacterial properties, ensuring that conclusions drawn are valid and reliable.

33) Agriculture:
Agriculture relates to the cultivation of plants and animals for food, fiber, and other products. The relevance of plant extracts in agriculture extends to pest management and improving plant health, showcasing how the antimicrobial properties of Juniperus squamata can be applied in agroecological practices.

34) Discussion:
Discussion refers to the section of research papers where results are analyzed and contextualized. In this study, discussions about the significance of the antibacterial properties and phytochemical compounds help interpret findings, draw conclusions, and propose future research directions based on observed outcomes.

35) Substance:
Substance relates to any material with a definitive chemical identity. The extracts from Juniperus squamata are substances studied for their medicinal properties, aiming to identify their active ingredients, which could provide benefits in treating infections and disease in humans and animals.

36) Fragrance:
Fragrance pertains to pleasant scents derived from plants. While not the focus of this study, the essential oils from Juniperus squamata also have applications in aromatherapy and herbal products, highlighting the importance of exploring all potential uses of medicinal plants.

37) Pesticide:
Pesticide refers to substances used to deter harmful organisms affecting plants. The study may imply that the antibacterial properties of Juniperus squamata could extend to development as natural pesticides, offering alternatives to synthetic chemicals in agricultural practices and promoting sustainable farming techniques.

38) Swelling:
Swelling refers to inflammation potentially alleviated by medicinal herbs. The traditional medicinal uses of Juniperus squamata include addressing swelling, indicating the plant's potential anti-inflammatory properties, which could be relevant for therapeutic applications in managing various health conditions related to inflammation.

39) Family:
Family refers to the taxonomic classification grouping related organisms. Juniperus squamata belongs to the Cupressaceae family, indicating its ecological relationships and potential common characteristics, including shared medicinal properties with other members, emphasizing the importance of botanical classification in pharmacognosy.

40) Powder:
Powder indicates the form of plant material processed for extraction. The leaves of Juniperus squamata were ground into powder to facilitate extraction of phytochemicals, crucial for subsequent analyses assessing the medicinal potential and antibacterial effects of the plant's constituents.

41) Cotton:
Cotton, in this context, likely refers to the sterile cotton swab used for inoculating bacteria on agar plates during the antibacterial assays. The use of sterile materials is vital in laboratory practices to prevent contamination and ensure accurate results in microbiological testing.

42) Delhi:
Delhi is significant as a hub for research and education in India. Its academic institutions contribute to the advancement of knowledge regarding medicinal plants. The recognition of Juniperus squamata's value in scientific studies emphasizes the importance of research centers located within urban settings.

43) Sugar:
Sugar, in the context of phytochemicals, is relevant as a potential energy source and a part of plant metabolism. Some phytochemicals from Juniperus squamata could contain sugar derivatives, affecting their biological activity and enhancing their potential use in nutritional applications.

44) Blood:
Blood is critical in human physiology, carrying oxygen and nutrients. The relevance of Juniperus squamata's compounds may extend to hematological health, potentially influencing circulatory functions or serving as therapeutic agents that can benefit conditions affecting blood composition and circulation.

45) Cutan:
Sudan, in this context, refers to Sudan III, a dye used for testing the presence of fats and oils in phytochemical analysis. The use of specific reagents like Sudan III highlights the methods employed to investigate various plant compound categories during the study.

46) Ripe:
Ripe refers to the optimal maturity of fruits from which essential oils can be extracted. The study mentions extracting from ripe berries, highlighting the significance of harvest timing for obtaining maximum phytochemical yield, important for the subsequent analyses of medicinal properties.

47) Pain:
Pain indicates a physical condition targeted for relief by various medicinal plants. The traditional use of Juniperus squamata in managing pain_symptoms emphasizes the plant's broader therapeutic potential, underscoring the need for rigorous testing of its analgesic properties.

48) Worm:
Worm, in this context, relates to parasitic infections possibly treatable with the antimicrobial properties of Juniperus squamata. The study's focus on the plant's potential efficacy against various pathogens expands its relevance in addressing gastrointestinal issues, particularly those caused by parasitic worms.

Other Science Concepts:

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Discover the significance of concepts within the article: ‘Himalayan juniper (Juniperus squamata): Phytochemical and antimicrobial assessment.’. Further sources in the context of Science might help you critically compare this page with similair documents:

Medicinal plant, Phytochemical, Essential oil, Therapeutic agent, Traditional medicine, Ayurvedic medicine, Medicinal value, Antibacterial activity, Phytochemical screening, Phytochemical analysis, Antimicrobial activity, Disc diffusion method, Zone of inhibition, Minimum inhibitory concentration, Active ingredient, Antimicrobial agent, Phytochemical Investigation, Secondary metabolite, Gram positive bacteria, Gram negative bacteria, Commercial exploitation, Alkaloids flavonoids, Qualitative phytochemical analysis, Ethanol extract, Methanol extract, Bacterial strain, Minimum bactericidal concentration, Chemical constituent, Steam distillation, Bacterial pathogen, Ferric chloride test, Himalayan plants, Primary metabolite, Wild medicinal plant, Presence of flavonoid.

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