Butanolide, tyrosol ester, and acetophenone from Streptomyces.
Journal name: World Journal of Pharmaceutical Research
Original article title: Butanolide, tyrosol ester and acetophenone derivatives from terrestrial streptomyces spp.
The WJPR includes peer-reviewed publications such as scientific research papers, reports, review articles, company news, thesis reports and case studies in areas of Biology, Pharmaceutical industries and Chemical technology while incorporating ancient fields of knowledge such combining Ayurveda with scientific data.
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Sayed A. Ahmed, Ebtehal Taha, Islam Koriem, Emadeldin M. Kamel
World Journal of Pharmaceutical Research:
(An ISO 9001:2015 Certified International Journal)
Full text available for: Butanolide, tyrosol ester and acetophenone derivatives from terrestrial streptomyces spp.
Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research
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Summary of article contents:
Introduction
In the exploration of natural products derived from microorganisms, the study discusses the chemical investigation of crude extracts from terrestrial Streptomyces spp. isolates (Ank150, 174, and 179). It highlights the discovery of four new compounds: 2-hydroxy-1-(4-hydroxy-3-methoxy-phenyl)-ethanone, 1-(3,4-dimethoxy-phenyl)-2-hydroxy-ethanone, acetic acid 2-(4-hydroxy-phenyl)-ethyl ester, and N-acetyltyrosol. The methodologies employed, such as spectroscopic analysis and chromatography, facilitated the identification of these new compounds, contributing to the understanding of natural product diversity and their potential for drug discovery.
Isolation of Compounds from Streptomycetes
The isolation of compounds from Streptomyces spp. involved a systematic approach to purification and characterization. The study leveraged silica gel chromatography, enabling the separation of the obtained crude extracts into distinct fractions. Among these, four compounds were identified for the first time from bacterial sources, and others were confirmed as known compounds. This meticulous process is crucial, as it underscores the importance of traditional techniques combined with modern analytical methods in the isolation and characterization of bioactive compounds from natural sources.
Spectroscopic Identification
The structures of the newly discovered compounds were elucidated using rigorous spectroscopic techniques, including 1D and 2D NMR, mass spectrometry, and UV/VIS spectroscopy. Each compound exhibited distinct spectral characteristics that allowed for their identification and confirmation. For instance, the first compound, 2-hydroxy-1-(4-hydroxy-3-methoxy-phenyl)-ethanone, showcased unique correlations in its HMBC spectrum that clarified its structural features. This detailed analysis not only validates the structures but also illustrates the complexity of natural products derived from microbial sources.
Biological Activity and Potential Applications
The study noted the biological activities exhibited by the isolated extracts against several bacterial strains, including Escherichia coli and Staphylococcus aureus. The findings indicate that the compounds derived from the studied Streptomyces spp. possess potential antibiotic properties, supporting their further exploration for therapeutic applications. Historical references to their use in traditional medicine emphasize their biological relevance and the need for ongoing research in natural product pharmacology.
Conclusion
In summary, this investigation highlights the significance of terrestrial Streptomyces spp. as a source of novel and bioactive natural products. The study successfully identifies four new compounds and elucidates their structures through advanced analytical techniques. Furthermore, the exhibited antibacterial activity suggests a promising avenue for future research focused on drug development from these microbial metabolites. This work contributes to the broader field of natural product chemistry and reinforces the value of microorganisms as vital resources in the quest for new therapeutic agents.
FAQ section (important questions/answers):
What are the new compounds derived from Streptomyces spp.?
Four new compounds were isolated: 2-hydroxy-1-(4-hydroxy-3-methoxy-phenyl)-ethanone, 1-(3,4-dimethoxy-phenyl)-2-hydroxy-ethanone, acetic acid 2-(4-hydroxy-phenyl)-ethyl ester, and N-acetyltyrosol. These compounds were identified through spectroscopic analysis.
Which microorganisms were investigated for secondary metabolites?
The study focused on terrestrial derived Streptomyces spp. isolates Ank150, Ank174, and Ank179, which are known for producing diverse secondary metabolites with potential therapeutic uses.
What techniques were used for isolating the compounds?
Compounds were isolated using techniques like silica gel chromatography, Sephadex LH-20 chromatography, RP18 purification, and NMR spectroscopy for structural characterization.
What biological activities were exhibited by the crude extracts?
The crude extracts exhibited activity against various bacteria, including Bacillus subtilis and Staphylococcus aureus, indicating potential antimicrobial properties of the derived compounds.
What were the known compounds identified in the extracts?
The known compounds identified included polyhydroxybutyric acid, virginiae butanolide B, reductomycin, and tyrosol, which have been previously documented in medicinal studies.
Who conducted the research detailed in the text?
The research was conducted by a team from the Chemistry Department, Faculty of Science, Beni Suef University, including Dr. Sayed A. Ahmed and his colleagues.
Glossary definitions and references:
Scientific and Ayurvedic Glossary list for “Butanolide, tyrosol ester, and acetophenone from Streptomyces.”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.
1) Table:
Table refers to a structured format used to display data and results in an organized manner. In the context of scientific publications, tables help summarize complex information like NMR assignments and molecular weights of compounds. They allow readers to quickly understand and compare experimental results or findings without having to wade through extensive text.
2) Medium:
Medium in scientific research often denotes the environment in which organisms grow or react, such as culture media for microorganisms. In this study, M2 medium was utilized for cultivating Streptomyces spp., essential for extracting metabolites. The choice of medium can significantly affect the yield and variety of compounds produced by these organisms.
3) Purification:
Purification in the context of chemistry and microbiology refers to the process of isolating compounds from a mixture to obtain a pure substance. It involves various techniques like chromatography, which was used here to separate and isolate the metabolites from Streptomyces spp. This ensures that the chemical structure and activity of the isolated compounds can be reliably determined.
4) Science (Scientific):
Science is the systematic enterprise that builds and organizes knowledge in the form of testable explanations and predictions about the universe. In this article, science encompasses the study of natural products from Streptomyces spp., contributing to our understanding of microbiology and potential development of new antibiotics, showcasing the relevance of scientific research to societal needs.
5) Egypt:
Egypt serves as the geographic and cultural context for this research, highlighting the local biodiversity of microorganisms such as Streptomyces. Conducting studies in specific locales like Egypt can unveil unique strains with potential for drug discovery, contributing to global knowledge while promoting the use of local resources in scientific endeavors.
6) Field:
Field refers to the area of study or research circle within which specific scientific inquiries occur. In this research, the field of natural product chemistry and microbiology overlap as scientists investigate compounds from Streptomyces spp. The findings contribute to a broader understanding of drug development within this scientific field, addressing public health issues.
7) Street:
Street refers to a physical location, notably the address of the Chemistry Department at Beni Suef University in Egypt, where the research was conducted. It emphasizes the importance of institutional infrastructure in scientific research, as collaboration with universities facilitates academic inquiry and innovation in medicinal chemistry and natural products.
8) Shalem (Śāḷēṃ, Śāleṃ):
Salem in this context refers to part of the address for the Chemistry Department, indicating a specific locality in Egypt. Address identifiers like Salem enhance the traceability of research origins, establishing credibility and contextual relevance for the scientific contributions made in the publication, and connecting researchers to their geographic roots.
9) Water:
Water is a critical solvent and medium for biochemical reactions in biological systems. In this study, water was involved in the extraction processes during the isolation of microbial metabolites. Understanding how water interacts with compounds in these processes affects extraction efficiency, influencing the types and quantities of metabolites obtained from Streptomyces spp.
10) Drug:
Drug refers to substances that are used for medicinal purposes, either to diagnose, cure, or alleviate symptoms of diseases. This article discusses the potential of newly isolated compounds from Streptomyces spp. as candidates for drug development, emphasizing the natural products' role in creating novel pharmaceuticals for treating various health conditions.
11) Measurement:
Measurement pertains to the quantification of physical or chemical properties, essential for validating experimental results. In this research, measurements of compound structures via NMR and mass spectrometry provide critical insights into their identity and properties, which are fundamental to evaluating their possible biological activities and pharmaceutical applications.
12) Discussion:
Discussion constitutes a section of scientific papers where results are interpreted and contextualized within the broader field. In this study, the discussion allows authors to synthesize findings regarding the new compounds derived from Streptomyces spp., considering their significance in antibiotic discovery and the implications for future research in natural product chemistry.
13) Antibiotic (Antibacterial):
Antibiotic refers to substances that inhibit or destroy bacteria, including compounds derived from natural sources like Streptomyces spp. The article emphasizes the significance of studying these microorganisms for potential antibiotic production, as traditional antibiotics face challenges like resistance, showcasing the need for continuous exploration of novel antibacterial agents.
14) Substance:
Substance describes a distinct chemical entity with specific properties and compositions. This research focuses on isolating various organic compounds (substances) derived from Streptomyces spp., highlighting the significance of identifying and characterizing these substances for potential applications in medicine, particularly in drug development and therapeutic interventions.
15) Transformation (Transform, Transforming):
Transform refers to the alteration of one state or structure into another. In the context of this research, it discusses the transformation of microbial metabolites into identified compounds through extraction and purification processes. Understanding these transformations helps elucidate pathways of natural product biosynthesis and their potential uses in therapeutic applications.
16) Medicine:
Medicine refers to the field dedicated to the diagnosis, treatment, and prevention of illness, heavily reliant on chemical compounds derived from nature. This study on Streptomyces spp. underscores the connection between natural products and medicine, illustrating how isolated compounds may lead to new therapeutic agents that contribute to public health.
17) Activity:
Activity signifies the biological effectiveness or reactivity of compounds, an essential aspect of pharmacology. The research highlights the biological activity of newly isolated compounds against various bacterial strains, illustrating their potential use as antimicrobial agents and contributing vital data towards drug efficacy assessment and therapeutic relevance.
18) Aureus:
Aureus refers to Staphylococcus aureus, a type of bacteria that is often studied in microbiology due to its clinical significance as a pathogen. In the article, the mention of Staphylococcus aureus provides context for the antibiotic testing of newly isolated compounds, showcasing their relevance in combating antibiotic-resistant infections.
19) Animal:
Animal denotes living organisms in the kingdom Animalia, often utilized in scientific research for understanding biological processes. In pharmacology, animal studies are crucial for assessing the safety and efficacy of new drugs before clinical use. This research indirectly highlights animal testing in the process of drug development from microbial sources.
20) Nagel:
Nagel refers to Macherey-Nagel, a company that produces laboratory supplies such as chromatography plates. This brand mentioned in the study emphasizes the utilization of high-quality materials in the research processes, reinforcing the importance of reliable tools and equipment in achieving reproducible and accurate scientific results during compound isolation.
21) Rich (Ṛch):
Rich in a scientific context often pertains to the diversity and abundance of resources available. Here, it highlights the biological richness of Streptomyces spp. in producing novel compounds, underlining the significance of biodiversity in drug discovery and the unique potential of these microorganisms for yielding biologically active substances.
22) Hand:
Hand may refer to the practical skills and manual techniques utilized in laboratory procedures. In research, 'hand-on' skills are essential for executing experiments effectively, emphasizing the researcher’s ability to handle instruments and materials competently, thus ensuring accurate results when isolating compounds from microbial extracts.
Other Science Concepts:
Discover the significance of concepts within the article: ‘Butanolide, tyrosol ester, and acetophenone from Streptomyces.’. Further sources in the context of Science might help you critically compare this page with similair documents:
Natural product, Molecular weight, Chemical screening, Acetic acid, Pharmaceutical Research, Spectral data, Structural elucidation, Spectroscopic analysis, Chemical investigation, Color reaction, Hydroxy group, New antibiotic, Cultivation condition.