Antioxidant activity of the leaves of alstonia scholaris r.br.

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Journal name: World Journal of Pharmaceutical Research
Original article title: Antioxidant activity of the leaves of alstonia scholaris r.br.
The WJPR includes peer-reviewed publications such as scientific research papers, reports, review articles, company news, thesis reports and case studies in areas of Biology, Pharmaceutical industries and Chemical technology while incorporating ancient fields of knowledge such combining Ayurveda with scientific data.
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Author:

M. Ayub Ali, L. Inaotombi Devi, C. Lalchhandama, and C. Lalmuanthanga


World Journal of Pharmaceutical Research:

(An ISO 9001:2015 Certified International Journal)

Full text available for: Antioxidant activity of the leaves of alstonia scholaris r.br.

Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research

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Summary of article contents:

Introduction

Alstonia scholaris, commonly known as Thumriat in Mizoram, is a significant evergreen tree in the Apocynaceae family, found extensively across India, particularly in deciduous and evergreen forests. The latex from this plant has traditional medicinal applications, including treating wounds and addressing earaches. The plant's extensive use in traditional medicine highlights its potential phytochemical properties, particularly its antioxidant effects which are crucial in the context of wound healing. This study investigates the antioxidant activity present in the methanolic extract of A. scholaris leaves using various in vitro methods.

Antioxidant Activity and Methods

The study employed three in vitro assay methods to evaluate the antioxidant activity of A. scholaris leaves: DPPH free radical scavenging activity, Ferric reducing antioxidant potential (FRAP) assay, and total phenolic content estimation. The DPPH method measured antioxidant capacity by assessing the ability of the extract to reduce the absorbance of a DPPH solution, indicating its radical scavenging ability. Results indicated a significant DPPH free radical scavenging activity of 23.26±0.79 mg Trolox equivalent (TE) per gram of dry leaves, underlining the extract's potential to mitigate oxidative stress.

Phytochemical Constituent Analysis

The Ferric reducing antioxidant potential (FRAP) assay and total phenolic content (TPC) estimation further supported the findings regarding antioxidant capacity. The FRAP assay demonstrated an antioxidant activity of 6.93±1.04 mg TE per gram of dry leaves, reflecting the extract's ability to reduce ferric ions to ferrous ions. Additionally, the total phenolic content was quantified at 0.79±0.14 mg gallic acid equivalent (GAE) per gram of dry leaves, confirming the presence of beneficial phenolic compounds known for their radical-scavenging properties. This aspect of the study emphasizes the role of phenolic compounds, as their antioxidant capabilities are crucial for promoting wound healing and reducing oxidative damage.

Impact on Wound Healing

The antioxidant properties of A. scholaris play a transformative role in wound healing. Reactive Oxygen Species (ROS), which accumulate during the inflammatory response to wounds, can induce cellular damage and hinder the healing process. Antioxidants present in A. scholaris extracts can neutralize these harmful ROS and reduce their detrimental effects on tissue repair. The relationship between antioxidant activity and wound healing is underscored in the study, suggesting that the high antioxidant content present in A. scholaris can significantly enhance healing processes while protecting cells from oxidative stress.

Conclusion

The findings of this study reveal that the methanolic extract of Alstonia scholaris possesses notable antioxidant properties, characterized by significant DPPH free radical scavenging activity, FRAP activity, and total phenolic content. These properties suggest the potential application of A. scholaris extracts in wound healing and as a natural source of antioxidants. The study highlights not only the therapeutic potential of this plant in traditional medicine but also advocates for its further exploration in antioxidant and anticancer research, underlining its important role in enhancing health outcomes through natural remedies.

FAQ section (important questions/answers):

What are the medicinal uses of Alstonia scholaris?

Alstonia scholaris has been utilized traditionally to treat wounds, boils, asthma, hypertension, lung cancer, pneumonia, and fever, mainly among Mizo tribes who apply its latex for earache and various ailments.

What methods were used to assess antioxidant activity?

The antioxidant activity was evaluated using three in vitro assays: DPPH free radical scavenging activity, Ferric Reducing Antioxidant Potential (FRAP) assay, and Total Phenolic content estimation.

What were the findings regarding DPPH radical scavenging activity?

The DPPH free radical scavenging activity of Alstonia scholaris leaves was measured at 23.26 ± 0.79 mg Trolox equivalent per gram of dry leaves, indicating significant antioxidant capacity.

How was the total phenolic content measured in the study?

Total phenolic content was estimated using the Folin-Ciocalteu method, resulting in a measurement of 0.79 ± 0.14 mg Gallic acid equivalent per gram of dry leaves.

What is the potential application of Alstonia scholaris based on the study?

The findings suggest that Alstonia scholaris leaves could be used as a natural antioxidant and a potent material for wound healing due to their significant antioxidant properties.

Which phytochemical constituents contribute to the antioxidant properties?

Phenolic compounds, including flavonoids and tannins, play a crucial role in the antioxidant activities of Alstonia scholaris by scavenging free radicals and protecting against oxidative damage.

Glossary definitions and references:

Scientific and Ayurvedic Glossary list for “Antioxidant activity of the leaves of alstonia scholaris r.br.”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.

1) Activity:
Activity refers to the measurable effect or performance of a specific treatment or substance, such as the antioxidant activity of the methanolic extract of Alstonia scholaris leaves. This activity is significant in biomedical research, particularly for evaluating the potential health benefits of plant extracts in areas like wound healing and disease management.

2) India:
India is the geographical context of the study, home to Alstonia scholaris, which is explored for its medicinal properties. As a tropical country, India boasts rich biodiversity and numerous indigenous plants utilized in traditional medicine, making it a vital region for ethnopharmacological studies and research on natural remedies.

3) Veterinary science:
Veterinary science relates to the field of medicine focused on animal health and care. This study's authors are affiliated with a veterinary college, underscoring the relevance of natural products in treating animal ailments, promoting healing, and managing diseases through herbal remedies derived from plants like Alstonia scholaris.

4) Inflammation:
Inflammation is a biological response to harmful stimuli, including pathogens and injuries, causing tissue damage. The study indicates that antioxidants play a crucial role in reducing inflammation, which can benefit wound healing by preventing further tissue injury and aiding in the recovery of inflamed areas.

5) Substance:
Substance refers to a particular matter or material with distinct properties. In this study, it highlights the extracts derived from Alstonia scholaris leaves as notable substances exhibiting antioxidant and healing properties. Identifying effective substances is essential in developing therapies based on natural products for various health conditions.

6) Evolution:
The term evolution in the context of this research pertains to the development of therapeutic practices over time, emphasizing the ongoing scientific discovery of plant-derived compounds. Acknowledging the evolution of knowledge regarding herbal medicine can inform modern approaches in healthcare, integrating traditional methods with contemporary research.

7) Science (Scientific):
Science encompasses the systematic study of the natural world, employing rigorous methodologies to understand phenomena. This research exemplifies the scientific approach to investigating the antioxidant properties and therapeutic potential of Alstonia scholaris, contributing to the broader understanding of the biological effects of plant extracts in health and medicine.

8) Study (Studying):
Study denotes a detailed examination or analysis of a specific subject. In this research, the study of Alstonia scholaris focused on assessing its antioxidant activity and potential health benefits. Such studies are critical for validating traditional medicinal claims and exploring new applications for natural substances in healthcare.

9) Accumulation (Accumulating, Accumulate):
Accumulation refers to the build-up of substances, typically relating to neutrophils and reactive oxygen species (ROS) at a wound site. In the study, it highlights the importance of antioxidants in counteracting the negative effects of ROS accumulation, which can hinder healing, thus underscoring the protective role of these compounds.

10) Flavonoid:
Flavonoid relates to a class of polyphenolic compounds commonly found in plants, known for their antioxidative properties. The study references flavonoids as constituents responsible for the antioxidant activity of Alstonia scholaris, demonstrating their significance in preventing oxidative stress and promoting healing in injury management.

11) Forest:
Forest is relevant not only as a habitat for Alstonia scholaris but also as a symbol of biodiversity in India. The forests provide numerous medicinal plants that are utilized in traditional practices. Understanding the ecological context of these plants can enhance conservation efforts and sustainable use of resources.

12) Family:
Family in this context refers to the taxonomic group Apocynaceae to which Alstonia scholaris belongs. Knowing the family classification aids researchers in understanding the plant’s characteristics and biochemical properties, facilitating the exploration of other related species that may share valuable therapeutic attributes.

13) Animal:
Animal signifies the scope of veterinary applications of plant-derived treatments. The research connects the studied plant's antioxidant properties to potential benefits in animal health, showcasing how natural remedies can aid in managing animal illnesses and promoting overall well-being through traditional knowledge integration.

14) Powder:
Powder indicates the physical state of the processed plant leaves for extraction in the research. Proper preparation of powdered plant material is essential for effective extraction of bioactive compounds. The powder form increases surface area, enhancing solvent interaction and improving yield during the maceration process.

15) Tree:
Tree specifies the botanical classification of Alstonia scholaris, emphasizing its status as a large evergreen tree. The characteristics of the tree, such as its latex and leaves, are fundamental to the research, as specific plant parts are often associated with different medicinal properties and therapeutic uses.

16) Human body:
Human body signifies the target of the therapeutic benefits derived from the studied antioxidant activity. Understanding how plant extracts affect human physiology, including mechanisms of action and healing processes, is crucial in developing effective treatments that leverage natural substances for improved health outcomes.

17) Transformation (Transform, Transforming):
Transformation denotes significant changes occurring within biological systems, such as neoplastic transformation due to oxidative stress. The study references transformation to emphasize the harmful effects of excessive reactive oxygen species, highlighting the protective role of antioxidants in preventing damage to cellular structures and maintaining health.

18) Phytochemical:
Phytochemical refers to the naturally occurring chemical compounds found in plants. The study identifies phytochemicals, like phenolic compounds, as key players in the antioxidant activity of Alstonia scholaris, which are instrumental in protecting cells from oxidative damage and promoting healing in various biological contexts.

19) Manjunatha (Mañjunātha):
Manjunatha is cited in the study as a reference for methodology in extracting plant materials. This highlights the importance of following established protocols and previous research in ensuring reliable, reproducible results. Citations enrich the study by connecting it to the broader scientific landscape.

20) Discussion:
Discussion represents the segment of the research that synthesizes findings and draws connections to existing literature. It provides critical insights into the implications of the antioxidant properties of Alstonia scholaris, facilitating a deeper understanding of its potential applications in medicine, particularly in wound healing.

21) Developing:
Developing indicates a focus on progressing research or practical applications in healthcare. The study looks toward developing natural remedies based on plant studies, especially pertinent in regions with rich biodiversity, where traditional knowledge meets modern scientific inquiry to address health challenges effectively.

22) Container:
Container refers to the storage mechanism for the extracted plant material. Proper storage in airtight containers is crucial for preserving the active compounds, preventing degradation over time. This practice ensures that the extracts retain their medicinal properties until they are further tested and utilized.

23) Disease:
Disease signifies the health conditions targeted by the potential therapeutic applications of Alstonia scholaris. By assessing antioxidant activity, the study aims to provide insights into its effectiveness in treating diseases associated with oxidative stress, positioning it as a candidate for traditional and modern medicinal use.

24) Species:
Species is a taxonomic term relevant to the biological classification of Alstonia scholaris. It emphasizes the uniqueness of the plant and its specific traits that contribute to its medicinal properties. The study contributes to the body of knowledge concerning different species and their potential benefits.

25) Yamuna (Yamunā, Yāmuna, Yāmunā, Yamuṉā):
Yamuna pertains to a geographical landmark in India where Alstonia scholaris can be found. Mentioning specific regions like the Yamuna reinforces the ecological context for the research and can drive interest in regional studies focusing on biodiversity and traditional uses of local flora.

26) Pankti (Paṅkti, Pamkti):
Pankti is another cited researcher whose work contributes context to the study. Referencing Pankti underlines the collaboration and continuity within scientific literature. It establishes credibility and shows the interconnected nature of research in evaluating the properties of Alstonia scholaris and related plants.

27) Bengal (Bemgal):
Bengal refers to a region in India known for its rich biodiversity, including numerous medicinal plants. Associating Alstonia scholaris with this area highlights the ecological and cultural richness that informs traditional medicine, encouraging more localized studies on plant-based therapies in diverse ecosystems.

28) Muslin:
Muslin pertains to the fabric used in the filtration process during the extraction of plant compounds. The use of muslin signifies practical, traditional approaches in laboratory techniques and underscores the importance of effective filtration methods in obtaining pure extracts for subsequent study.

29) Cancer:
Cancer indicates a critical health issue that could be addressed through antioxidants found in Alstonia scholaris. Researching natural antioxidants can lead to alternative treatment options for cancer, showing the plant's potential as a complementary asset in cancer therapies through its medicinal properties.

30) Bitter:
Bitter relates to the taste profile of Alstonia scholaris latex, which is often associated with certain medicinal properties in traditional practices. Understanding flavor profiles in plants can be indicative of their biochemical constituents and hints at their usage in folk medicine or dietary applications.

31) Killing (Killed):
Killed refers to the detrimental effects of excessive reactive oxygen species leading to cell death. In the study, this highlights the importance of antioxidants in preventing oxidative stress-induced cellular damage, illuminating their role in wound healing and maintaining the viability of essential cells.

32) Table:
Table refers to the organized representation of data summarizing the findings of the study. Utilizing tables enhances the clarity and accessibility of information, enabling readers to quickly grasp key results related to antioxidant content and compare the findings against established standards.

33) Field:
Field signifies the specific area of research and application related to medicinal plants, particularly in context to wound healing and antioxidant activity. This study contributes knowledge to the field, connecting traditional uses of plants with contemporary scientific investigation in natural product research.

34) Fever:
Fever denotes a common symptom that the Mizo tribes treat using Alstonia scholaris. It reinforces the plant's role in traditional medicine and highlights the relevance of studying its properties, potentially identifying new therapeutic approaches for managing fever and related health conditions.

35) Devi (Devī):
Devi refers to one of the co-authors involved in the study, representing collaborative efforts in scientific research. Each author's contribution plays a significant role in shaping the study's methodology and results, showcasing teamwork essential for comprehensive scientific investigation.

36) Hair:
Hair refers to the specific physical characteristic of the seeds of Alstonia scholaris. The presence of hair on the seeds may be linked to ecological adaptations and can be relevant in seed dispersal strategies, emphasizing the intricate relationships between botanical features and environmental dynamics.

37) Drug:
Drug pertains to the implications of the antioxidant properties of Alstonia scholaris leaves for pharmaceutical development. By demonstrating beneficial effects, the study suggests that plant extracts could serve as a basis for creating natural drugs or supplements, integrating botanical knowledge into contemporary medicinal practices.

Other Science Concepts:

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Discover the significance of concepts within the article: ‘Antioxidant activity of the leaves of alstonia scholaris r.br.’. Further sources in the context of Science might help you critically compare this page with similair documents:

Alstonia scholaris, Natural remedies, Wound healing, Antioxidant activity, Antioxidant properties, Reactive oxygen species, Oxidative damage, Total phenolic content, Ethnomedicinal plant, Antioxidant, Reactive oxygen species (ROS), Hydrogen donating ability, Methanolic extract, Total antioxidant capacity, Wound healing activities, Plant Extract, Hyaluronic acid, Absorbance measurement, DPPH free radical scavenging activity, DPPH free radical scavenging, Neoplastic transformation, Phenolic compound, Plant Derived Compound, Standard curve calibration.

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