Effect of Portulaca oleracea on blood and plasma in Wistar rats.
Journal name: World Journal of Pharmaceutical Research
Original article title: Effect of fraction 1 of portulaca oleracea on hematological and plasma biochemical parameters in male wistar rats
The WJPR includes peer-reviewed publications such as scientific research papers, reports, review articles, company news, thesis reports and case studies in areas of Biology, Pharmaceutical industries and Chemical technology while incorporating ancient fields of knowledge such combining Ayurveda with scientific data.
This page presents a generated summary with additional references; See source (below) for actual content.
Original source:
This page is merely a summary which is automatically generated hence you should visit the source to read the original article which includes the author, publication date, notes and references.
Oyedeji Kazeem Olusina, Adegoke Olajire, Oladosu Adebayo, Abidoye Dele
Download the PDF file of the original publication
World Journal of Pharmaceutical Research:
(An ISO 9001:2015 Certified International Journal)
Full text available for: Effect of fraction 1 of portulaca oleracea on hematological and plasma biochemical parameters in male wistar rats
Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research
Copyright (license): WJPR: All rights reserved
Summary of article contents:
Introduction
Portulaca oleracea, a widely distributed fleshy annual herb, has been studied for its potential hematological and biochemical effects. This study examines the impact of Fraction 1 of Portulaca oleracea on male Wistar rats, investigating both the toxic and beneficial outcomes of the plant’s extracts. The research involved a series of experiments, including the preparation of methanol extracts, chromatographic fractionation, and bioassays to evaluate blood parameters after treatment with different doses of Fraction 1.
Effect on Hematological Parameters
The study revealed that Fraction 1 of Portulaca oleracea significantly impacted the hematological parameters of treated rats. At the highest dose (3 mg/kg), there was a significant increase in packed cell volume (PCV) and red blood cell (RBC) values, indicating potential stimulation of erythropoiesis. Conversely, lower doses (1 mg/kg and 2 mg/kg) resulted in significant reductions in PCV and RBC values, suggesting a possible induction of anemia. The findings indicate that the fraction may influence the production and metabolism of erythrocytes, highlighting the dual effects of the extract depending on the dosage administered.
Plasma Biochemical Analysis
In addition to hematological effects, the study assessed plasma biochemical parameters, noting significant increases in total protein, albumin, and globulin levels in response to Fraction 1 treatment at doses of 2 mg/kg and 3 mg/kg. Elevated total protein levels may enhance the blood's buffering capacity and colloid osmotic pressure, while increased albumin reinforces the transport of various substances in the bloodstream. Furthermore, the rise in globulin levels suggests a potential boost in both natural and acquired immunity, underscoring the extract's favorable impact on plasma biochemistry.
Liver Enzyme Activity
The research also evaluated the activity of liver enzymes, specifically alanine aminotransferase (ALT) and aspartate aminotransferase (AST). Significant increases in the activities of these enzymes across all doses administered indicated potential hepatic damage or necrosis. Elevated enzyme levels are typically associated with cell damage, particularly in the liver, highlighting the need for caution in using this plant extract, as it may pose toxic risks alongside its beneficial effects on hematological parameters.
Conclusion
Overall, the findings of this study suggest that Fraction 1 of Portulaca oleracea possesses both toxic and beneficial effects on hematological functions and blood chemistry in male rats. While it demonstrates potential in enhancing certain blood parameters and plasma biochemistry, the study also raises concerns regarding liver enzyme activity, indicating possible hepatic damage. Due to these mixed effects, consumers and researchers alike are urged to exercise moderation in the utilization of Portulaca oleracea, especially given the unknown impacts on human health.
FAQ section (important questions/answers):
What is the main objective of the study on Portulaca oleracea?
The study aimed to investigate the effects of chromatographic fraction 1 of Portulaca oleracea on hematological and plasma biochemical parameters in male Wistar rats.
What extraction method was used for Portulaca oleracea in this study?
The plant was cold-extracted in methanol for 72 hours to obtain crude extracts, which were then subjected to column chromatography for fractionation.
How were the male rats treated in the experiment?
Twenty male rats were divided into four groups, receiving various doses of fraction 1 orally for 30 days. Control group received distilled water.
What significant hematological changes were observed with fraction 1 treatment?
Fraction 1 at 3 mg/kg significantly increased red blood cell (RBC) counts and packed cell volume (PCV) levels, indicating potential enhancements in erythropoiesis and oxygen-carrying capacity.
What were the plasma biochemical effects of fraction 1 treatment?
Treatment with fraction 1 significantly increased total protein, albumin, and globulin levels, suggesting enhanced buffering capacity and immunity in the rats.
What is the conclusion regarding the safety of Portulaca oleracea consumption?
Fraction 1 showed both toxic and beneficial effects; therefore, moderation in Portulaca oleracea consumption is advised until further studies are conducted on humans.
Glossary definitions and references:
Scientific and Ayurvedic Glossary list for “Effect of Portulaca oleracea on blood and plasma in Wistar rats.”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.
1) Blood:
Blood is a vital bodily fluid that transports oxygen, nutrients, and waste products throughout the body. It plays an essential role in maintaining homeostasis and defending against pathogens. In the context of the study, blood parameters such as Red Blood Cell count and plasma biochemistry were evaluated to understand the effects of Portulaca oleracea on hematological functions.
2) Relative:
The term 'relative' in this context refers to comparisons made between the experimental group (treated rats) and the control group (untreated rats). These comparisons help in assessing the effectiveness of the treatment. Statistical significance is determined, indicating whether the observed effects are likely due to the treatment or chance.
3) Animal:
Animals, specifically male Wistar rats in this study, serve as model organisms for research into human health. They provide insights into biological processes and the effects of substances, which can help to understand complications in drug effects, toxicology, and potential therapeutic benefits before human testing occurs.
4) Male (Mālē):
The specification 'male' indicates that only male rats were used in the study. This is important because biological differences between male and female organisms can influence the results of pharmacological and toxicological studies. Using only male subjects ensures uniformity and reduces variability in the data related to gender differences.
5) Medicine:
Medicine encompasses the science and practice of diagnosing, treating, and preventing diseases. The findings of this study contribute to medicinal knowledge by indicating the potential effects of Portulaca oleracea on hematological parameters, suggesting its possible therapeutic or toxic properties, which are important for pharmaceutical applications.
6) Science (Scientific):
Science represents the systematic study of the structure and behavior of the physical and natural world through observation and experimentation. This study is rooted in scientific inquiry as it aims to understand the biochemical and hematological impacts of a natural compound, contributing to broader scientific knowledge in pharmacology and toxicology.
7) Water:
Water is often used as a control substance in biological experiments, serving as a solvent or vehicle for treatment administration. In this study, distilled water was used for the control group, allowing researchers to determine how the treatment with Portulaca oleracea affected the animals compared to a non-treated state.
8) Activity:
Activity refers to the biological effects observed in the study, specifically the influence of fraction 1 of Portulaca oleracea on various hematological and biochemical parameters. Measuring these activities, such as the concentration of specific proteins or enzyme levels, provides insights into the physiological impacts of the treatment on the rats.
9) Table:
Tables present data in a structured format, summarizing results such as hematological and biochemical parameters for different dosage groups. They facilitate easier comparison and interpretation of results, showcasing significant differences observed through statistical analysis, which is crucial for validating the study's findings and conclusions.
10) Study (Studying):
The study is a systematic investigation designed to assess the effects of fraction 1 of Portulaca oleracea on rats. It provides essential insights into the plant's potential therapeutic and toxicological properties and contributes to the understanding of its safe use in traditional medicine clinics.
11) Toxicity:
Toxicity refers to the degree to which a substance can cause harm to biological systems. This study explores the toxicological aspects of Portulaca oleracea by assessing various blood parameters and determining safe consumption limits, helping in the safe application of the plant in medicinal practices.
12) Language:
Language is the means by which scientific findings are communicated among researchers and within the medical community. Clear and precise language in the study is essential to articulate methodologies, results, and implications. It ensures that findings can be effectively interpreted, critiqued, and applied in practice or further research.
13) Azadirachta indica:
Azadirachta indica, commonly known as neem, is a plant known for its medicinal properties similar to those of Portulaca oleracea. This study references Azadirachta indica to establish comparisons with other herbal extracts, illustrating the broader context of natural products in therapeutic applications and their varied hematological effects.
14) Discussion:
The discussion section in research papers interprets the results, placing them in context with existing literature. It offers insights into the biological significance of the findings, potential mechanisms of action for the plant extracts studied, and their implications for future research or clinical applications.
15) Substance:
Substance refers to any chemical or material being tested, in this case, the chromatographic fraction 1 of Portulaca oleracea. Understanding the properties and effects of the substance is crucial for determining its therapeutic benefits or risks when used in medicinal practices.
16) Disease:
Disease refers to a pathological condition that alters body function. Understanding hematological and biochemical changes provides insights into how natural remedies like Portulaca oleracea may assist in disease prevention or treatment, potentially influencing clinical applications for various health conditions.
17) Family:
The term family in botanical classification categorizes related plants sharing common characteristics. Portulaca oleracea belongs to the Portulacaceae family. Understanding plant families aids in identifying potential medicinal properties shared among related species, thus guiding research on new therapeutic compounds.
18) Ghana (Ghanā, Ghāṇa, Ghāna):
Ghana is mentioned in the study as a geographical location where Portulaca oleracea is traditionally used for medicinal purposes. This highlights the cultural significance and potential historical therapeutic applications of the plant, reinforcing the need for scientific investigation into its effects.
19) House:
House refers to the controlled environment where the animals were bred and kept for experimentation. Proper housing conditions are crucial for ensuring the health and well-being of experimental subjects, which ultimately affects the validity and reliability of the scientific study's results.
20) Sida (Sīdā, Shida):
Sida, another plant referenced in the study, serves as a comparative point to highlight similar research findings within the domain of herbal remedy effects. This illustrates the interconnectedness of various plant studies in understanding their roles in physiology and potential therapeutic applications.
21) Tank (Ṭaṅk, Taṅk):
Tank refers to the container used for developing thin-layer chromatography (TLC) plates in the methodology. The tank environment is vital for achieving the necessary conditions for separating components within the extract, facilitating the purification process critical for gathering pure substances for testing.
Other Science Concepts:
Discover the significance of concepts within the article: ‘Effect of Portulaca oleracea on blood and plasma in Wistar rats.’. Further sources in the context of Science might help you critically compare this page with similair documents:
Portulaca oleracea, Statistical analysis, Control group, Experimental design, Significant reduction, Significant change, Thin Layer Chromatography, Animal model, Wound healing activity, Aqueous extract, Hematological Parameters, Significant decrease, Hemoglobin concentration, Duncan's multiple range test, Diuretic, Significant increase, Male Wistar rats, Methanolic extract, One-way analysis of variance, Acute toxicity test, Packed cell volume, Macrocytic anemia, Mean corpuscular hemoglobin, Bioassay, Toxic effect, Mean corpuscular hemoglobin concentration, Alanine amino transferase, Serum total protein, Aspartate Transaminase, Spectrophotometry, Aspartate Amino Transferase, Cold extraction method, Fractionation, Total Protein Concentration, Chromatographic fractionation, Experimental animal, Fraction 1, Plasma biochemical parameters, Total White Blood Cell, Red Blood Cell, Albumin concentration, Globulin concentration, Extraction technique, Clinical chemistry, Beneficial effect, Chromatographic procedure, Plasma level, Male Albino Rat, Blood Chemistry, Hemocytometer, Colloid osmotic pressure, Nutrition recommendation.