In vitro rapid multiplication of solanum trilobatum l. from shoot tip explant

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Journal name: World Journal of Pharmaceutical Research
Original article title: In vitro rapid multiplication of solanum trilobatum l. from shoot tip explant
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Author:

S. Subbaiya, S. Alagumanian, G. Jahirhussain, T. Nagarajan


World Journal of Pharmaceutical Research:

(An ISO 9001:2015 Certified International Journal)

Full text available for: In vitro rapid multiplication of solanum trilobatum l. from shoot tip explant

Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research

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Summary of article contents:

Introduction

Solanum trilobatum L., a perennial shrub of the Solanaceae family, is known for its medicinal properties and is commonly found in Southern India. This plant has long been used in traditional medicine to address various ailments, including respiratory disorders and tuberculosis. The current study aimed to develop an efficient protocol for the in vitro regeneration of S. trilobatum by utilizing shoot tip explants cultured on Murashige and Skoog (MS) basal medium supplemented with different concentrations of growth regulators. The overarching goal was to establish a reliable method for mass propagation of this medicinal plant to support conservation efforts and meet increasing demand.

In Vitro Shoot Multiplication

A critical focus of this research was the rapid multiplication of S. trilobatum shoots from explants. The study specifically evaluated the effects of two cytokinins, BAP (6-benzylaminopurine) and KIN (6-furfurylaminopurine), on shoot induction from shoot tip explants cultured on MS medium. The results indicated that BAP was more effective than KIN in promoting shoot proliferation. A maximum shoot induction frequency of 100% and an average of 8.4±1.51 shoots per explant were achieved at 2.0 µM/L BAP concentration. Meanwhile, 1.5 µM/L KIN yielded a significant average of 6.4±1.81 shoots. This demonstrates the critical role of cytokinins in enhancing shoot multiplication in in vitro cultures.

Root Induction Techniques

Following successful shoot multiplication, the next phase involved root induction of the isolated shoots. The plantlets were transferred to MS basal medium supplemented with various concentrations of auxins such as IBA (Indole butyric acid) and NAA (Naphthalene acetic acid). The study assessed the effectiveness of these hormones in promoting root formation. The experiments indicated that the optimal conditions for root induction were critical for the survival and successful acclimatization of plantlets. Rooting improvements are essential for ensuring the propagation process yields viable plants that can be transplanted into the soil.

Hardening and Acclimatization

The research also highlighted the importance of hardening and acclimatization of the regenerated plantlets. Successful transplantation to field conditions demands that in vitro-raised plants adapt to ex vitro environments. The hardened plantlets were gradually introduced to soil conditions after being maintained under controlled humidity levels. This step is pivotal in ensuring that the plants survive in natural habitats, particularly since established plants face the challenges of different environmental factors that were absent in the sterile culture environment.

Conclusion

The study successfully established a micropropagation protocol for S. trilobatum, demonstrating that axillary buds could be efficiently utilized for shoot multiplication and subsequent rooting under controlled in vitro conditions. The findings revealed that BAP is particularly effective in enhancing shoot proliferation, while appropriate auxin use is crucial for root development. The research contributes significantly to the conservation and rapid propagation of this important medicinal plant, addressing both its traditional uses and the need for sustainable cultivation practices amid increasing demand. The outcomes set the foundation for further studies on optimizing in vitro conditions to benefit other medicinal species as well.

FAQ section (important questions/answers):

What is the best cytokinin concentration for shoot induction?

The best cytokinin concentration for maximum shoot induction was found to be 2.0 µM/L BAP, which produced an average of 8.4±1.51 shoots per explant.

What plant species was studied for micropropagation?

The study focused on the micropropagation of Solanum trilobatum L., a medicinal plant used in traditional medicine for treating various diseases.

How were the explants prepared for the study?

Explants were surface sterilized by washing with detergent, followed by alcohol and mercuric chloride, then rinsed with sterile distilled water to ensure aseptic conditions.

What role do cytokinins play in shoot multiplication?

Cytokinins, specifically BAP and KIN, promote shoot multiplication by stimulating lateral meristem formation, enhancing the regeneration process in plant tissue culture.

What was the rooting protocol after shoot multiplication?

After shoot development, shoots were transferred to MS medium supplemented with IBA or NAA for root induction, where root number and length were measured.

What is the significance of micropropagation for medicinal plants?

Micropropagation allows for efficient, large-scale production of disease-free, genetically uniform plants, addressing the depletion of wild populations and ensuring availability of medicinal plants.

Glossary definitions and references:

Scientific and Ayurvedic Glossary list for “In vitro rapid multiplication of solanum trilobatum l. from shoot tip explant”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.

1) Medium:
In the context of plant micropropagation, 'medium' refers to the nutrient-rich formulation that supports plant tissue growth. It provides essential inorganic salts, carbon sources, vitamins, and growth regulators to facilitate the in vitro culture of the explants. The choice of medium directly influences the success of shoot and root induction.

2) Activity:
'Activity' in this study reflects the biological functions or responses elicited by Solanum trilobatum extracts, such as its medicinal properties. It encompasses aspects like antimicrobial, antidiabetic, and anticancer effects, indicating the functional efficacy of the plant in traditional medicine, thus highlighting its therapeutic potential.

3) Disease:
In this research, 'disease' pertains to health conditions that Solanum trilobatum can address, such as respiratory issues, bronchial asthma, and tuberculosis. Understanding its role in treating these ailments emphasizes the significance of studying medicinal plants for developing effective, natural remedies, contributing to healthcare and wellness.

4) India:
'India' is the geographical and cultural context where Solanum trilobatum is prevalent. The country's vast biodiversity and rich traditions of herbal medicine underscore the relevance of local flora in treating various ailments. The study highlights the importance of regional plants in traditional and contemporary medicine practices in India.

5) Water:
Water is a critical component of the culture medium for in vitro plant propagation. It serves as a solvent for nutrients and growth regulators, facilitating their absorption by explants. Adequate water supply ensures optimal growth conditions, influencing survival rates and the overall success of the micropropagation process.

6) Study (Studying):
'Study' refers to the systematic investigation conducted to develop an efficient micropropagation protocol for Solanum trilobatum. It involves examining the effects of various growth regulators and environmental conditions on plant regeneration, aiming to establish reliable methods for mass propagation of this valuable medicinal plant.

7) Medicine:
In this context, 'medicine' encompasses the therapeutic uses of Solanum trilobatum in treating various health conditions. It signifies the plant's role within traditional and modern medicinal practices, illustrating the integration of phytotherapy into healthcare systems for effective disease management and promotion of wellness.

8) Field:
'Field' denotes the natural environment where Solanum trilobatum is found and also the site for collecting explants for the study. The field context emphasizes the ecological aspects of plant growth, biodiversity, and the potential for sustainable harvesting of medicinal plants in their native habitats.

9) Salt (Salty):
'Salt' refers to the mineral components within the culture medium, specifically in the Murashige and Skoog (MS) medium, which provides essential nutrients for plant growth. The presence of salts aids in the osmotic balance and facilitates the uptake of nutrients, crucial for successful in vitro cultivation.

10) Species:
'Species' is a taxonomic classification representing Solanum trilobatum, highlighting its unique characteristics and significance within the Solanaceae family. The knowledge of species classification assists researchers in understanding biodiversity, conservation efforts, and the medicinal potential inherent in specific plant species.

11) Family:
'Family' indicates the broader taxonomic category to which Solanum trilobatum belongs, specifically Solanaceae. This classification relates to the shared botanical traits and behaviors of various genera and species within the family, linking the study of this plant to its relatives with similar medicinal properties.

12) Bronchial asthma:
'Bronchial asthma' refers to a chronic respiratory condition that can potentially be alleviated by Solanum trilobatum. The plant's traditional use in treating respiratory disorders emphasizes its relevance in herbal medicine, presenting an opportunity for scientific validation of its efficacy against respiratory ailments.

13) Phytochemical:
'Phytochemical' denotes the active chemical compounds found in Solanum trilobatum, such as alkaloids and flavonoids, which contribute to the plant's medicinal properties. The study of these compounds provides insights into the mechanisms of action and therapeutic effects, supporting the development of plant-based medicines.

14) Tuberculosis:
Tuberculosis is a bacterial infection affecting the lungs, which Solanum trilobatum has been historically used to treat. The plant's medicinal usage highlights the importance of exploring traditional remedies for modern therapeutic applications, particularly in areas with high prevalence of such diseases.

15) Tamilnadu (Tamil-nadu, Tamilnāḍu):
'Tamilnadu' is the state in India where Solanum trilobatum is commonly found. The region's rich biodiversity and traditional medicinal practices underscore the cultural significance of local plants in healthcare. It provides a framework for understanding the ethnobotanical relevance of species like Solanum trilobatum.

16) Surface:
'Surface' refers to the outer layers of plant tissues that require sterilization for successful in vitro culture. Proper surface treatment of explants is essential to prevent microbial contamination, ensuring a sterile environment for the initiation of plant tissue culture and successful regeneration.

17) Cotton:
'Cotton' is used here to describe the plugs used to cap culture tubes, maintaining an aseptic environment during plant tissue culture. This material facilitates gas exchange while preventing contamination, thus playing a vital role in ensuring the success of in vitro propagation techniques.

18) Botany:
'Botany' is the scientific study of plants, which encompasses the research conducted on Solanum trilobatum. The discipline involves understanding plant biology, taxonomy, and ecology, contributing to the knowledge base for its medicinal applications and the importance of plant conservation.

19) Drug:
'Drug' refers to the potential pharmaceutical applications derived from Solanum trilobatum. Investigating the plant's medicinal properties may lead to the discovery or development of new drugs, contributing to modern medicine by providing alternative therapies based on natural compounds.

20) Soil:
'Soil' refers to the medium into which successful plantlets are eventually transferred after cultivation in vitro. It represents the importance of natural substrates for sustaining plant growth, emphasizing the transition from laboratory conditions to real-world cultivation environments for herbal medicine usage.

21) Southern India:
'Southern India' is the geographical region where Solanum trilobatum is most commonly found. The distinct climate and biodiversity of this area form the backdrop for the study of local flora, reflecting the cultural and medicinal significance of native plants in traditional practices.

22) Sanskrit:
'Sanskrit' refers to the ancient language in which many traditional medicinal texts were written, thus providing historical context to Solanum trilobatum's use in Ayurveda. Understanding these texts can further validate the plant's role in traditional medicine and its relevance in contemporary herbal practices.

23) Siddha (Siddhā):
'Siddha' is an ancient Indian system of medicine that employs herbal remedies, including Solanum trilobatum, to treat diseases. This system's holistic approach to health exemplifies the cultural connection between indigenous practices and the use of local flora for therapeutic purposes in Southern India.

24) Alarka (Ālarka):
'Alarka' is the Sanskrit name for Solanum trilobatum, illustrating its cultural significance within traditional medicine. This nomenclature emphasizes the importance of understanding local names and uses, fostering a greater appreciation for the plant's role in regional healthcare practices.

25) Cancer:
'Cancer' refers to the group of diseases characterized by uncontrollable cell growth, which some extracts from Solanum trilobatum have shown potential in treating. Research into the plant's anticancer properties highlights the importance of evaluating natural compounds for effective cancer therapies.

26) Sugar:
'Sugar' indicates the carbon source (sucrose) used in the culture medium for plant tissue propagation. Providing energy for growing cells is essential for successful growth and development of plantlets, emphasizing the role of carbon sources in plant tissue culture protocols.

27) Table:
'Table' refers to the data presentation format used to summarize results from the experiments on shoot induction and root development. Tables facilitate comparative analysis of different treatments, aiding in the interpretation of results related to the influence of growth regulators on plant propagation.

28) Tamil (Tamiḻ):
'Tamil' is the language spoken in Tamilnadu, where Solanum trilobatum is commonly found, highlighting its cultural and regional significance. Understanding local languages fosters effective communication of traditional uses and values of the plant within the context of its medicinal properties.

29) Life:
'Life' encompasses the biological and ecological significance of Solanum trilobatum within its natural habitat. The plant plays a role in local ecosystems while also contributing to human health through its medicinal applications, emphasizing the interconnectedness of life forms within ecosystems.

30) Lamp:
'Lamp' signifies the use of a flame source (e.g., spirit lamp) during sterilization procedures within the lab to maintain aseptic conditions. This step is critical for preventing contamination during tissue culture, underscoring the meticulous approach taken in plant propagation research.

31) Snake poison (Snake venom):
'Snake poison' refers to the traditional use of Solanum trilobatum in treating snake bites. This highlights the significance of the plant in folklore medicine, suggesting a rich historical context for its applications in emergency health interventions based on local expertise.

32) Vallikantakarika (Vallikaṇṭakārikā, Valli-kantakarika):
'Vallikantakarika' is the Sanskrit name associated with the medicinal properties of Solanum trilobatum, reflecting its place in traditional texts and its application in Ayurvedic medicine. This historical nomenclature underscores the cultural continuity in herbal traditions and contemporary scientific inquiries.

33) Civilization:
'Civilization' pertains to the development of societies that utilize plants like Solanum trilobatum for medicinal purposes. The connection between cultural practices and plant usage for health reflects the intertwined evolution of human societies and their understanding of natural resources for wellness.

34) Chemotherapy:
'Chemotherapy' refers to the modern medical treatment for cancer using drugs to kill or slow growth of cancer cells. Understanding how Solanum trilobatum may enhance or complement chemotherapy treatments reveals the relevance of natural products in enhancing cancer care and patient outcomes.

35) Agnidamani (Agnidamanī, Agni-damani):
'Agnidamani' is a Sanskrit term linked to the medicinal properties of Solanum trilobatum, indicating its cultural significance in traditional medicine. This term encapsulates the revered relationship between indigenous practices and the natural remedies utilized for healing purposes across generations.

36) Flavonoid:
'Flavonoid' indicates a class of phytochemicals present in Solanum trilobatum that may possess antioxidant and anti-inflammatory properties. Researching these compounds enhances the understanding of the plant’s therapeutic potential, guiding future explorations into plant-derived medicine and phytotherapy.

37) Ayurveda (Āyurveda, Ayus-veda):
'Ayurveda' is the traditional system of Indian medicine that emphasizes natural healing methods, including the use of plants like Solanum trilobatum. The integration of herbal remedies into Ayurvedic practices illustrates the profound cultural reliance on local flora for health and wellness.

38) Relative:
'Relative' refers to the botanical relationships between Solanum trilobatum and other species within the Solanaceae family. Studying these relationships aids in understanding genetic diversity, ecological roles, and potential uses of related species in traditional and modern medicine.

39) Vitiation (Vitiated):
'Vitiated' describes an imbalance or disturbance in the body, often addressed in traditional medicine systems. The use of Solanum trilobatum to pacify vitiated conditions underscores its relevance in treatments aimed at restoring homeostasis in the body according to Ayurvedic principles.

40) Marathi (Marāṭhī, Marāthi):
'Marathi' is the language spoken in Maharashtra, indicating the linguistic diversity within India. This highlights the cultural variations in the nomenclature for medicinal plants like Solanum trilobatum, which may influence local practices and beliefs about its health benefits.

41) Kannada (Kannaḍa):
'Kannada' is one of the languages spoken in parts of Southern India, reflecting the cultural heritage and linguistic diversity of the region. Understanding the local names and uses of Solanum trilobatum in Kannada showcases the plant's significance across different communities.

42) Village:
'Village' denotes the rural contexts where traditional use of medicinal plants prevails. In villages, knowledge of plants like Solanum trilobatum for health treatments is often rooted in cultural practices and passed down through generations, signifying the importance of community wisdom in herbal medicine.

43) Habitat:
'Habitat' describes the natural environments where Solanum trilobatum is found, emphasizing its ecological preferences. Understanding the habitat supports conservation efforts and informs sustainable practices for harvesting the plant, thereby safeguarding its medicinal potential for future generations.

44) Mineral:
'Mineral' indicates the inorganic nutrients provided in the culture medium, essential for plant growth. Minerals play a crucial role in cellular functions, ensuring proper physiological processes in developing plant tissues during in vitro propagation.

45) Science (Scientific):
'Science' reflects the systematic study and research approach applied to investigate Solanum trilobatum. Scientific inquiry into its properties, uses, and regeneration methods provides a foundation for validating traditional knowledge and advancing sustainable practices in herbal medicine.

46) Nature:
'Nature' encompasses the organic environment where Solanum trilobatum exists, highlighting the importance of biodiversity. The study of natural plants for medicinal purposes emphasizes the need for conservation while promoting the understanding of ecosystem interplay and the benefits of sustainably utilizing natural resources.

47) Sowing:
'Sowing' refers to the planting of seeds or seedlings, connecting back to the eventual goal of the research to propagate Solanum trilobatum successfully. This process emphasizes the cultivation aspect, highlighting the agricultural dimensions of medicinal plant usage.

48) Powder:
'Powder' indicates the form in which plant material extracts are often prepared for use in herbal remedies. The process of converting raw plant materials into powdered form facilitates easy dosage and incorporation into various medicinal preparations, highlighting its practical applications.

49) Manure:
'Manure' refers to organic matter used to enrich soil for plant growth. Its role in providing nutrients emphasizes the importance of soil quality for successful cultivation, linking sustainable practices to the propagation of medicinal plants like Solanum trilobatum.

50) Pitta (Pītta, Piṭṭā):
'Pitta' denotes one of the three doshas in Ayurveda that governs metabolic processes in the body. The use of Solanum trilobatum to pacify vitiated pitta reflects its significance in maintaining balance and health according to traditional Ayurvedic principles on wellness.

51) Kapha:
'Kapha' describes another dosha in Ayurvedic medicine associated with bodily structure and stability. Solanum trilobatum's role in balancing kapha conditions underscores its relevance in traditional health practices, demonstrating the intricate connections between herbal medicine and holistic health.

52) Karur:
'Karur' is a district in Tamil Nadu known for its biodiversity, including the presence of Solanum trilobatum. This locality exemplifies the regional importance of medicinal plants in traditional practices, reinforcing the link between geographic areas and their flora's cultural significance.

53) Edema (Oedema):
'Edema' refers to swelling caused by fluid accumulation, which Solanum trilobatum may assist in treating. The plant's use in traditional medicine highlights its potential benefits for managing such conditions, underscoring the importance of studying local flora for therapeutic purposes.

54) Oriya (Oriyā):
'Oriya' is the language spoken in Odisha, reflecting cultural diversity. The nomenclature and usage of Solanum trilobatum in different languages signify its widespread recognition and importance in traditional herbal practices across various Indian states.

55) Male (Mālē):
'Male' refers to gender aspects in traditional medicine, particularly regarding male reproductive health. Solanum trilobatum has applications in enhancing male fertility, emphasizing the cultural significance of gender-specific health issues addressed by indigenous plant remedies.

56) Genu (Geṇu):
'Genu' refers to knee in anatomical terms, which may be relevant in discussing various healing practices. Although less directly tied to Solanum trilobatum, it highlights the potential for diverse applications of medicinal plants in treating musculoskeletal issues.

57) Tree:
'Tree' signifies the larger plant classification compared to herbs, placing Solanum trilobatum within the broader context of plant diversity. Understanding the differences between trees and shrubs aids in grasping ecological dynamics and interactions among varied plant forms in their habitats.

58) Crop:
'Crop' denotes cultivated plants for agricultural purposes. Solanum trilobatum may be promoted as a medicinal crop, highlighting the diversification of agricultural practices to include plants not only for food but also for health, encouraging sustainable practices in farming.

59) Rich (Ṛch):
'Rich' refers to the abundance of biodiversity available in regions like Southern India where Solanum trilobatum is found. The richness of local flora highlights the potential for discovering new medicinal plants and enhancing knowledge on their diverse applications.

60) Wall:
'Wall' signifies the physical structures present in micropropagation setups, such as culture vessels. While not directly relating to the plant, it underscores the controlled environments necessary for successful plant growth during in vitro studies, contributing to overall research methodologies.

61) Hand:
'Hand' refers to the manual skills required in plant tissue culture processes, from handling explants to performing inoculation. The human ability to cultivate and propagate plants emphasizes the significance of skillful techniques in achieving successful micropropagation outcomes.

62) Ghi (Ghī, Ghee):
'Ghee' is clarified butter used in traditional medicine, particularly in Siddha and Ayurveda. Its relevance in conjunction with Solanum trilobatum for treatments underscores the holistic approach of combining various natural products for therapeutic efficacy in herbal medicine.

63) Worm:
'Worm' pertains to parasitic conditions potentially treated by Solanum trilobatum, emphasizing the plant's broad applications in addressing various health issues. Understanding its medicinal roles highlights the importance of research in validating traditional uses of plants for contemporary healthcare solutions.

64) Shand (Śaṇḍ, Sand):
'Sand' refers to the soil composition used in growing media for plants such as Solanum trilobatum. Its inclusion in potting mixtures reflects the importance of soil structure, drainage, and aeration for sustaining healthy plant growth, crucial for successful cultivation practices.

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Solanum trilobatum, Traditional usage, Traditional medicine, Herbal medicine, Morphological features, Experimental design, Economic viability, Phytochemical screening, Antimicrobial activity, Traditional healer, Siddha System, Secondary metabolite, Duncan's multiple range test, Phytochemical studies, Distilled water, Acclimatization, Micropropagation, Explant, Plant tissue culture, Tissue culture technique, World Journal of Pharmaceutical Research, Culture medium, Gelling agent, Survival rate, Culture condition, Solanum trilobatum L., In vitro regeneration, Hormone concentration, Nutrient media, Clonal propagation, Different concentration, Medicinal agent, Growth regulator, Plant growth regulator, In vitro organogenesis, Basal medium, High frequency, In vitro multiplication, Normal laboratory conditions.

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