In vitro propagation of Bacopa monnieri from nodal explants.
Journal name: World Journal of Pharmaceutical Research
Original article title: In vitro mass propagation of bacopa monnieri (linn.) wettst from nodal explant - a multipurpose medicinal plant
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T. Nagarajan, S. Alagumanian, G. Jahirhussain, S. Subbaiya
World Journal of Pharmaceutical Research:
(An ISO 9001:2015 Certified International Journal)
Full text available for: In vitro mass propagation of bacopa monnieri (linn.) wettst from nodal explant - a multipurpose medicinal plant
Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research
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Summary of article contents:
Introduction
Bacopa monnieri, commonly known as Brahmi or water hyssop, is a highly valued medicinal plant belonging to the Scrophulariaceae family. It has been traditionally used in Ayurvedic medicine for its cognitive-enhancing properties, including memory enhancement and anxiety reduction. However, due to overharvesting from its natural habitats to meet pharmaceutical demands, there is a pressing need for sustainable propagation methods. The study conducted by Nagarajan et al. focuses on developing a reliable in vitro mass propagation technique using nodal explants of Bacopa monnieri. The research utilizes plant tissue culture techniques to facilitate year-round propagation of this crucial medicinal plant.
In Vitro Mass Propagation Techniques
The research explores the in vitro cultivation of nodal explants on Murashige and Skoog (MS) medium supplemented with various concentrations of growth regulators, specifically cytokinins like BAP (6-benzylaminopurine) and KIN (6-furfurylaminopurine). The study found that BAP was significantly more effective for shoot multiplication compared to KIN. Notably, a concentration of 2.0 μM BAP achieved a 100% shoot induction rate, producing an average of 6.4 shoots per nodal explant, with an average shoot length of 5.02 cm. These findings demonstrate the potential for effectively utilizing nodal explants from Bacopa monnieri in tissue culture applications for rapid propagation.
Root Induction and Plantlet Establishment
After successful shoot multiplication, the isolated shoots were transferred to MS medium with varying concentrations of auxins such as IBA (Indole Butyric Acid) and NAA (Naphthalene Acetic Acid) for root induction. The auxin concentrations ranged from 2.0-10.0 μM. The study documents that the rooting efficiency was crucial for the successful transition of plantlets from an in vitro environment to soil. The hardened rooted plantlets were subsequently acclimatized to field conditions, which underscores the importance of thorough hardening processes to ensure successful establishment and growth in a natural environment.
Significance of Micropropagation
Micropropagation is recognized as a vital tool in the conservation and sustainable utilization of medicinal plants. This technique not only enables the mass propagation of Bacopa monnieri but also supports the production of pathogen-free, genetically uniform plantlets at a competitive cost. The study emphasizes the role of micropropagation in addressing the challenges posed by habitat loss and overexploitation of medicinal plants. By ensuring a continuous supply of quality plant material, micropropagation can play a crucial role in meeting the rising demand for herbal medicines.
Conclusion
In conclusion, the study conducted by Nagarajan et al. highlights the successful in vitro mass propagation of Bacopa monnieri through nodal explants, showcasing the effectiveness of cytokinins and auxins in shoot and root induction. This research not only contributes to the field of plant biotechnology by establishing viable propagation protocols but also emphasizes the need for sustainable practices in the utilization of medicinal plants. By adopting such biotechnological techniques, the preservation of biodiversity and the maintenance of medicinal plant resources can be better achieved, ensuring their availability for future generations.
FAQ section (important questions/answers):
What is Bacopa monnieri and its main uses?
Bacopa monnieri, also known as Brahmi, is a medicinal herb used primarily for enhancing cognitive function, reducing anxiety, and improving memory formation. It's traditionally used in Ayurveda.
How was the mass propagation of Bacopa monnieri achieved?
Mass propagation was achieved by culturing nodal explants on Murashige and Skoog (MS) medium, supplemented with cytokinins BAP and KIN, which facilitated multiple shoot induction and growth.
Which growth regulator was more effective for shoot multiplication?
Among the tested cytokinins, BAP was more effective than KIN, with 2.0 µM BAP yielding the highest number of shoots (6.4±1.94) and optimal shoot length.
What conditions were maintained for the culture growth?
The cultures were kept at 25±2°C under a 16-hour photoperiod, with a light intensity of 30-40 µM m-2 s-1, providing optimal conditions for plant growth.
How were the explants sterilized before culturing?
Explants were surface sterilized using detergent, followed by 70% alcohol and 0.1% mercuric chloride, then rinsed thoroughly with sterile distilled water to eliminate contaminants.
What were the results of root induction for the plantlets?
Root induction was achieved using MS medium supplemented with auxins like IBA and NAA, resulting in healthy rooted plantlets, which were later acclimatized and transplanted.
Glossary definitions and references:
Scientific and Ayurvedic Glossary list for “In vitro propagation of Bacopa monnieri from nodal explants.”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.
1) Medium:
In the context of plant tissue culture, 'medium' refers to the nutrient-rich solution that provides the necessary growth environment for plant cells to develop. The study utilized Murashige and Skoog (MS) medium, supplemented with vitamins and growth regulators like BAP and KIN, essential for the in vitro propagation of Bacopa monnieri.
2) Water:
Water is a crucial component in plant cultivation, serving as the primary solvent for nutrients and facilitating various physiological processes. In micropropagation, sterile water is used to wash explants and prepare culture media, ensuring a contamination-free environment for optimal growth and development of plantlets in laboratory conditions.
3) Study (Studying):
The 'study' encompasses the systematic investigation into the in vitro mass propagation of Bacopa monnieri, focusing on nodal explants. It evaluates different concentrations of growth regulators and their effects on shoot and root induction, ultimately contributing to plant tissue culture methodologies and enhancing the propagation of medicinal plants.
4) Drug:
Drugs derived from medicinal plants are of significant interest in pharmacology. Bacopa monnieri is noted for its various therapeutic properties, such as cognitive enhancement and antioxidant activities. The study highlights the importance of this plant in the pharmaceutical industry and the need for sustainable cultivation methods to meet drug demands.
5) Medicine:
The term 'medicine' refers to the substances used to treat diseases and maintain health. Bacopa monnieri is traditionally used in Ayurveda and Siddha systems of medicine for its cognitive benefits. The research emphasizes the role of plant-derived medicines in primary healthcare, especially among rural populations reliant on herbal remedies.
6) Brahmi (Brāhmī):
Brahmi is a commonly used name for Bacopa monnieri, recognized for its cognitive-enhancing properties. The ancient Ayurvedic texts recommend its use to promote brain health and improve memory. The study aims to facilitate the sustainable propagation of Brahmi to support its continued use in traditional and modern medicine.
7) India:
India is a hub of biodiversity and traditional medicine, home to many medicinal plants, including Bacopa monnieri. The study acknowledges the rich heritage of herbal medicine in India, where plants are integral to practices like Ayurveda, and highlights the necessity of conserving and propagating these valuable species.
8) Field:
The 'field' refers to the natural habitat where Bacopa monnieri is found. Due to overharvesting and habitat loss, natural populations of this medicinal plant are declining. The study aims to establish in vitro propagation methods that can replenish field populations and ensure the sustainable availability of Brahmi.
9) Salt (Salty):
In the context of tissue culture, 'salt' refers to the inorganic nutrients essential for plant growth, such as those found in Murashige and Skoog (MS) medium. These salts provide the necessary elements for cellular processes and are critical for the successful in vitro cultivation of plant explants.
10) Surface:
Surface sterilization is a crucial step in plant tissue culture, ensuring that explants are free of contaminants. The study utilized methods like washing with detergent and alcohol to prepare nodal explants for culture. Proper surface sterilization increases the chances of successful plant propagation in vitro.
11) Science (Scientific):
Scientific refers to the objective, systematic study of phenomena, in this case, pertaining to plant biology and propagation methods. The research employs scientific methodologies to explore the principles of in vitro culture, contributing valuable insights into effective propagation techniques for medicinal plants like Bacopa monnieri.
12) Family:
Bacopa monnieri belongs to the Scrophulariaceae family, which consists of various species known for their medicinal properties. The study emphasizes the importance of understanding taxonomic relationships within plant families to better implement conservation and propagation strategies for valuable medicinal plants.
13) Cotton:
In the context of culture media, 'cotton' refers to the cotton plugs used to cover culture tubes, providing a sterile environment while allowing airflow. This practice is essential for preventing contamination during plant tissue culture, as described in the experimental methods of the study.
14) Indian:
The term 'Indian' signifies the cultural and geographical context of the study. Bacopa monnieri, also known as Brahmi, is indigenous to India, and its extensive use in traditional Indian medicine highlights the significance of preserving and propagating this plant for future generations
15) Botany:
Botany, the study of plants, is central to the research on Bacopa monnieri. This field examines plant biology, taxonomy, and ecology, providing essential knowledge for developing sustainable propagation techniques via in vitro cultures, that support conservation and potential commercial applications.
16) Soil:
Soil provides essential nutrients, water, and support for plant life. The study discusses hardening plantlets by transferring them to a soil mixture to acclimatize them from in vitro conditions to natural environments. This transition is critical for ensuring the survival and growth of propagules in the field.
17) Azadirachta indica:
Azadirachta indica, commonly known as Neem, is another important medicinal plant in India. Though the study focuses on Bacopa monnieri, the mention of Neem highlights the diversity of traditional medicinal plants in Indian flora and the significance of plant propagation research for sustainable health care solutions.
18) New Delhi:
New Delhi, the capital of India, represents a significant hub for research and education in medicinal plant studies. As the study may contribute to academic and practical aspects of traditional medicine, recognizing the importance of urban centers like New Delhi facilitates research and collaboration in plant science.
19) Transformation (Transform, Transforming):
Transformation refers to the genetic alteration of plants for improved traits. While the study does not focus on genetic transformation, understanding these methods can enhance the efficiency of propagating Bacopa monnieri through advancements in biotechnology, leading to healthier and more resilient plant varieties.
20) Tamilnadu (Tamil-nadu, Tamilnāḍu):
Tamilnadu refers to the state in South India where Bacopa monnieri thrives and where the research is conducted. The state's rich biodiversity and traditional knowledge greatly influence the study's focus, emphasizing the importance of localized studies in understanding and conserving valuable plants.
21) Ayurveda (Āyurveda, Ayus-veda):
Ayurveda is a traditional Indian system of medicine that emphasizes the use of herb-based remedies for health and wellness. Bacopa monnieri is a prominent herb in Ayurveda, recommended for cognitive enhancement. The study highlights the importance of conserving such Ayurvedic plants through sustainable propagation practices.
22) Varanasi (Vārāṇasī, Varanashi, Vārāṇasi, Varaṇasī, Varāṇasī, Vāraṇasī, Vāraṇāsī):
Varanasi is a significant cultural and spiritual city in India, associated with ancient Ayurvedic practices. Its mention in the study context emphasizes the historical relevance of places where traditional medicine was formulated and highlights the ongoing need for research into the propagation of medicinal plants like Bacopa monnieri.
23) Sanskrit:
Sanskrit is the classical language of ancient Indian texts, including those on Ayurveda and herbal medicine. The relevance of Sanskrit in the study lies in its role in preserving ancient wisdom about medicinal plants like Bacopa monnieri, thus emphasizing the need for continuous research and documentation.
24) Samhita (Saṃhitā, Saṃhita):
Samhita refers to ancient collections of texts, particularly those in Ayurveda. The inclusion of this term highlights the historical importance of scriptural knowledge in understanding medicinal plants such as Bacopa monnieri and their applications. Research aims to facilitate the utilization of such knowledge in modern practices.
25) Gujarat:
Gujarat is a state known for its rich biodiversity and traditional medicinal practices in India. While the study centers on Bacopa monnieri, the mention of Gujarat underscores the geographical diversity of medicinal plant studies in India, emphasizing the need for widespread propagation efforts across different regions.
26) Quality:
Quality assurance in medicinal plant research is crucial for ensuring effective therapies. The study addresses the importance of standardization and characterization of Bacopa monnieri to ensure that products derived from it meet safety and efficacy standards, thereby enhancing its therapeutic potential.
27) Habitat:
Habitat refers to the natural environment where Bacopa monnieri grows. The study highlights habitat loss due to overharvesting, emphasizing the critical need for conservation and in vitro propagation techniques to protect this valuable medicinal plant and ensure its availability for future generations.
28) Species:
Species classification is vital for understanding the biological characteristics of Bacopa monnieri. The study centers around this specific species within the Scrophulariaceae family, highlighting the significance of research aimed at propagation and conservation efforts to maintain biodiversity in medicinal plants.
29) Tamala (Tamāla):
Cinnamomum tamala, also known as Tamala, is another species with medicinal properties. Including it in the study's context highlights the diversity of plants studied for their pharmacological benefits, paralleling the research focus on Bacopa monnieri and emphasizing the importance of comprehensive research on various medicinal species.
30) Sthana (Sthāna):
Sthana translates to 'place' in Sanskrit and often refers to significant locations in Ayurvedic texts. Its mention underscores the importance of traditional knowledge as foundational to understanding the meanings and uses of medicinal plants and the practices surrounding their propagation and application in healthcare.
31) Delhi:
As the capital city, Delhi plays a central role in educational and healthcare initiatives related to medicinal plants. The city's relevance in the research on Bacopa monnieri underscores the connection between urban centers and the traditional medicinal plant discourse in India, enhancing community access to scientific knowledge.
32) Table:
In research, tables are used to present data systematically. Table 1 in the study summarizes the effects of various concentrations of cytokinins on shoot induction from nodal explants, facilitating comparison and analysis of results, which is crucial for drawing conclusions and understanding the propagation dynamics.
33) Tamil (Tamiḻ):
Tamil refers to the language spoken in Tamil Nadu, the region where this research was conducted. Understanding local languages can enhance communication about the significance of traditional medicinal practices, and it allows for broader community engagement in conservation efforts and the promotion of native medicinal plants like Bacopa monnieri.
34) Nadu (Nāḍu):
Nadu signifies 'land' or 'region' in Tamil and is often used to denote states in India, including Tamil Nadu. This regional reference emphasizes the rich cultural heritage and biodiversity found in Tamil Nadu, critically informing the research on local medicinal plants and their sustainable propagation.
35) Neem:
Neem, or Azadirachta indica, is a significant medicinal tree native to India. Though the focus of the study is on Bacopa monnieri, the inclusion of Neem underscores the diverse array of plants researched in India, highlighting the importance of understanding various plants for holistic medicinal applications.
36) Tree:
In the context of this study, 'tree' evokes the broader category of plants, including those such as Neem or Cinnamomum tamala. Recognizing trees and their roles in traditional medicine emphasizes the need for conservation and propagation work across varied plant types to ensure medicinal resources remain available.
37) Lamp:
The lamp symbolizes the aseptic conditions required during the inoculation in tissue culture. It represents safety protocols in microbiology where flame sterilization is used, highlighting the importance of maintaining sterile environments in scientific research to prevent contamination of plant cultures.
38) Cult:
Cult usually refers to cultivation practices, particularly in the context of plant nursery operations. The study explores 'in vitro' methods for cultivating Bacopa monnieri, showcasing modern cult practices that are essential for mass propagation and conservation of medicinal plants through controlled conditions.
39) Cinnamomum tamala:
Cinnamomum tamala, or Tamala Patra, is another important medicinal plant mentioned alongside Bacopa monnieri. Its inclusion in the research context highlights the variety of beneficial plant species found in India, emphasizing the diverse applications of plant-based therapies being studied and preserved.
40) Plumbago zeylanica:
Plumbago zeylanica is another medicinal plant referenced in the context of plant propagation techniques. The mention highlights the diverse approaches being developed in in vitro studies, drawing parallels with Bacopa monnieri and reflecting the broader significance of research in preserving various medicinal species.
41) Pharmacognostical:
Pharmacognostic studies involve the evaluation of medicinal plants for quality, safety, and efficacy. The research into Bacopa monnieri incorporates such evaluations, with the aim of ensuring that the propagated plants meet required standards for use in traditional and modern medicinal practices.
42) Aegle marmelos:
Aegle marmelos, commonly known as Bael, is another significant medicinal plant. The mention of this plant alongside Bacopa monnieri enriches the research’s narrative about the diversity in Indian medicinal plants and underscores the importance of similar propagation studies to support traditional medicine.
43) Mandukaparni (Maṇḍūkaparṇī, Manduka-parni, Mamdukaparni):
Mandukaparni is another name for Bacopa monnieri, emphasizing its identity in traditional contexts. Including this term reinforces the cultural richness surrounding the plant and highlights its recognized importance in Ayurvedic practices and in the ongoing research for enhancing its propagation.
44) Vayasthapana (Vayasthāpana, Vaya-sthapana):
Vayasthapana refers to a classification in Ayurveda for herbs that maintain youth and vigor. It directly relates to the traditional use of Bacopa monnieri in enhancing cognitive function and memory, situating the plant within significant Ayurvedic practices that this research aims to preserve.
45) Tamalapatra (Tamālapatra, Tamala-patra):
Tamala-patra refers to the leaves of Cinnamomum tamala, often used in traditional culinary and medicinal applications. The mention of this term shows the interconnectedness of various plants in traditional medicine and highlights the importance of preserving knowledge and practices associated with them.
46) Discussion:
Discussion refers to the analytical portion of research where results are interpreted and contextualized. In this study, the discussion allows for reflection on the findings related to the in vitro propagation of Bacopa monnieri, offering insights on implications for conservation and practical applications in the field.
47) Meditation:
Meditation is a practice associated with mental focus and tranquility, historically linked to the use of Bacopa monnieri in enhancing concentration. Its mention underscores the cultural significance of this medicinal plant in traditional practices aimed at cognitive health and mental well-being.
48) Relative:
In the context of species classification, 'relative' refers to the relationship between different species within the same taxonomic family. The study examines Bacopa monnieri's relatives within the Scrophulariaceae family, which can provide insights into its growth characteristics and conservation strategies.
49) Epilepsy:
Epilepsy is a neurological condition where Bacopa monnieri is traditionally used for its potential therapeutic effects. Mentioning epilepsy highlights the importance of researching the medicinal properties of Bacopa monnieri, reinforcing its relevance in treating various cognitive and neurological disorders.
50) Cutting:
In plant propagation, 'cutting' refers to a method used to grow new plants from sections of stems or leaves. Although the study focuses on nodal explants for Bacopa monnieri propagation, understanding other methods like cuttings is essential for diversifying propagation techniques in medicinal plant cultivation.
51) Disease:
Disease refers to any condition that impairs normal functioning, where medicinal plants often play a crucial role in treatment. The study of Bacopa monnieri aims at understanding its efficacy in treating cognitive diseases and its potential place in modern pharmacotherapy for various health conditions.
52) Mineral:
Mineral nutrients are vital for plant growth and health. In the study, minerals are provided through the MS medium formulation, playing an essential role in cellular functions and overall plant development during the in vitro propagation process aimed at enhancing Bacopa monnieri cultivation.
53) Siddha (Siddhā):
Siddha is a traditional South Asian system of medicine similar to Ayurveda, utilizing various herbal remedies. The mention of Siddha in relation to Bacopa monnieri emphasizes the plant's usage in different traditional medicine systems, reinforcing the need for research into its propagation and therapeutic applications.
54) Bhalla:
Bhalla is likely a reference to a contributor or researcher in the field mentioned within citations in the study. It signifies the collaborative nature of scientific research, highlighting how multiple experts contribute to the body of knowledge related to propagating medicinal plants like Bacopa monnieri.
55) Kannan (Kannaṉ, Kaṇṇaṉ, Kaṉṉaṉ, Kaṉṉāṉ):
Kannan most likely refers to a researcher noted for their contributions to the study. Recognizing individual researchers underscores the collaborative efforts in scientific inquiries involving plant propagation techniques and emphasizes the importance of shared expertise in the ongoing exploration of medicinal botanicals.
56) Nausea:
Nausea is a potential side effect linked to the consumption of Bacopa monnieri on an empty stomach. The mention emphasizes the need for safe herbal use research, reinforcing the importance of understanding dosage and methods that ensure the health benefits of medicinal plants without adverse side effects.
57) Nature:
Nature relates to the natural environment in which medicinal plants grow. The study acknowledges the critical role of understanding natural habitats to develop effective propagation strategies that can sustainably harvest and conserve plant species like Bacopa monnieri while maintaining biodiversity.
58) Sowing:
Sowing refers to the planting of seeds, an essential step in traditional agriculture. Although the study focuses on in vitro propagation, understanding sowing techniques enriches the dialogue about sustainable practices for growing and maintaining crops of medicinal plants like Bacopa monnieri.
59) Saxena (Sakshena, Saksena):
Saxena is likely a reference to a co-author or researcher associated with the study. Acknowledging contributions from various authors highlights the interdisciplinary approach to plant research and the collective effort to explore the applications of medicinal plants in health and wellness.
60) Pigeon:
Pigeon generally refers to a bird species but in this context, it could denote another research perspective. Its relevance may relate to ecological studies or traditions that involve wildlife in relation to the habitat of medicinal plants like Bacopa monnieri.
61) Manure:
Manure is organic material applied to soil to enhance its nutrient content. The study invokes manure in the context of hardening plantlets, emphasizing its role in supporting healthy growth and development of propagated medicinal plants whenever they are transitioned into the soil.
62) Patra (Pātra):
Patra translates to 'leaf' in Sanskrit and often denotes important herbal components in traditional medicine. The mention of 'Patra' signifies the significant role of plant parts in pharmaceutical preparations, highlighting the need for research on their properties and conservation in the study of medicinal plants.
63) Shiva (Śivā, Sivā, Śiva, Siva, Sīva):
Siva likely denotes another researcher or contributor to the study. Acknowledging an array of authors emphasizes the collaborative nature of contemporary plant research, encouraging a shared commitment to advancing our understanding and cultivation of important medicinal species like Bacopa monnieri.
64) Simha (Siṃha):
Sinha refers to an author or collaborator associated with the research on Bacopa monnieri. Recognizing names within the context of the study highlights the value of team efforts in scientific inquiry and the shared goal of advancing knowledge in medicinal plant propagation.
65) Varga (Vargā):
Varga refers to classifications or categories in Ayurvedic texts, often related to medicinal plants. Its mention signifies the traditional context in which Bacopa monnieri is utilized and emphasizes the importance of integrating traditional knowledge with scientific research for the effective use of medicinal plants.
66) Kumar (Kumār):
Kumar likely references an author or researcher contributing to the study. Highlighting individual contributors emphasizes the collaborative effort behind research in plant science, showcasing the shared goal of knowledge advancement regarding vital medicinal species like Bacopa monnieri.
67) Karur:
Karur refers to a town in Tamil Nadu, possibly where research is conducted or relevant to the historical context of medicinal plant usage. The mention supports the geographical diversity of studies focused on propagating native medicinal species important to regional health practices.
68) Chang:
Chang likely refers to another researcher or contributor involved in the study. Acknowledging diverse authors underscores the breadth of expertise contributing to scientific research concerning plant propagation, revealing the collective desire to advance methods for cultivating significant medicinal plants.
69) Sudha (Sudhā):
Sudha likely denotes a researcher or contributor within the context of the study. Acknowledging the diverse backgrounds of authors enriches the narrative of collaborative scientific inquiry, emphasizing that knowledge about plant propagation is built through ongoing contributions from multiple experts.
70) Birth:
Birth relates to the origins or inception of ideas and practices. Its mention signifies the historical significance of medicinal plant research and the need to trace back traditional uses of plants like Bacopa monnieri, aiming to cultivate them sustainably in present times.
71) Veda (Vedā):
Vedic refers to the ancient scriptures of Hinduism that outline various aspects of life, including medicine. The mention emphasizes the deep historical roots of medicinal practices using plants like Bacopa monnieri, reinforcing the need for research that bridges traditional wisdom with contemporary applications.
72) Guru:
Guru refers to a teacher or spiritual guide, especially in Hindu traditions. In the context of Bacopa monnieri’s traditional use, the term invokes the importance of wisdom passed through generations regarding the herb’s efficacy in enhancing cognitive functions and meditation practices.
73) Raja (Rāja, Rājā):
Raja likely refers to a contributor or researcher involved in the study. Acknowledging individual contributors enhances the understanding of collective effort in advancing knowledge and research in plant science, making valuable contributions towards the sustainable propagation and application of traditional medicinal plants.
74) Sita (Sītā, Śīta, Sīta, Shita, Sītā, Sitā, Śita, Śītā, Siṭa, Siṭā):
Sita may reference a contributor or concept within the context of the study. It symbolizes the intertwining of cultural narratives and scientific research on medicinal plants, highlighting the cultural dimensions that support the understanding and utilization of plants like Bacopa monnieri in health applications.
75) Rama (Ramā, Rāmā, Rāma):
Rama likely signifies another collaborator involved in the research. Recognizing individual contributors illustrates the cooperation within the scientific community to deepen the understanding of plant propagation methods and stresses the importance of teamwork in advancing medicinal plant studies.
[Note: The above list is limited to 75. Total glossary definitions available: 80]
Other Science Concepts:
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Kin, Baap, Medicinal plant, Herbal medicine, Seasonal variation, Statistical analysis, Phytochemistry, In vitro, Nootropic, Aseptic environment, Antioxidant activities, Bacopa monnieri, Duncan's multiple range test, Adaptogen, Micropropagation, Explant, Quality-control method, Shoot length, MS medium, Adaptive mechanisms, Memory formation, Sucrose concentration, Pharmaceutical product, Drug standardization, Growth regulator, Plant growth regulator, Auxin, Basal medium, Active medicinal constituents, Ancient Ayurvedic Text, Agricultural technology, Plant cell culture, Standard culture conditions.