Antimicrobial activity of Salicornia brachiata against pathogens
Journal name: World Journal of Pharmaceutical Research
Original article title: In-vitro antimicrobial activity of salicornia brachiata (roxb.) against selected pathogens
The WJPR includes peer-reviewed publications such as scientific research papers, reports, review articles, company news, thesis reports and case studies in areas of Biology, Pharmaceutical industries and Chemical technology while incorporating ancient fields of knowledge such combining Ayurveda with scientific data.
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Prasanna Lakshmi K., Lakshmi Narayana V. and G. M. Narasimha Rao
World Journal of Pharmaceutical Research:
(An ISO 9001:2015 Certified International Journal)
Full text available for: In-vitro antimicrobial activity of salicornia brachiata (roxb.) against selected pathogens
Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research
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Summary of article contents:
Introduction
The study investigates the in vitro antimicrobial activity of Salicornia brachiata (Roxb.), a halophyte found in mangrove habitats along the coasts of India. Salicornia species have gained attention due to their potential use in various applications, including as food, paper production, and edible oil extraction. This research focuses on evaluating the antimicrobial properties of S. brachiata extracts against several pathogenic microorganisms, including both plant and human pathogens. The plant parts were collected, dried, and extracted using different solvents (hexane, chloroform, methanol, and water) to identify their antimicrobial efficacy.
Antimicrobial Activity of Plant Extracts
The extraction process revealed varying degrees of antimicrobial activity among the different solvent extracts of S. brachiata. The study highlights that the highest concentration of 500 mg/ml consistently yielded greater inhibition of microbial growth. Specifically, the hexane extracts exhibited limited antifungal activity with no effect on bacterial strains. Conversely, chloroform and methanol extracts showed moderate antimicrobial activity against both bacterial and fungal pathogens, indicating the potential efficacy of these solvents in extracting bioactive compounds from the plant. The methanol extract demonstrated its highest antimicrobial activity against Saccharomyces cerevisiae, while chloroform extracts displayed activity against various tested strains, including Bacillus subtilis and Lactobacillus acidophilus.
Water Extracts Displaying Significant Antimicrobial Properties
Interestingly, water extracts of S. brachiata exhibited the highest antimicrobial effectiveness across a broader range of microorganisms tested. This extract demonstrated significant inhibitory effects against important bacterial strains such as Bacillus megaterium and Klebsiella pneumonia, and fungal strains, including Candida albicans and Rhizopus stolonifer. The results suggested that water extracts have a more potent overall antimicrobial profile compared to hexane, chloroform, and methanol extracts. This reinforces the need to consider water-soluble compounds in the pursuit of natural antimicrobial agents.
Implications for Pharmacological Applications
The study indicates that the antimicrobial properties of S. brachiata could lead to potential applications in pharmaceutical development. The findings suggest that halophytic plants, especially S. brachiata, may serve as novel sources for discovering new antimicrobial agents that can address infections caused by antibiotic-resistant pathogens. Secondary metabolites produced by mangroves and halophytes might reveal unique chemical structures suitable for pharmaceutical formulations, providing an opportunity for integrating traditional medicine with modern health practices.
Conclusion
In summary, Salicornia brachiata displays promising antimicrobial activity against various pathogens, particularly with its water extracts. The research underscores the importance of utilizing halophytes in exploring new options for antimicrobial agents that can combat resistant strains. The antimicrobial properties found in this study could have significant implications for the development of novel treatments for infectious diseases, emphasizing the potential of halophytic plants in the pharmaceutical industry. Further research is warranted to isolate and identify the specific bioactive compounds responsible for these effects, paving the way for innovative therapeutic applications.
FAQ section (important questions/answers):
What is Salicornia brachiata and where is it found?
Salicornia brachiata, also known as 'Sea asparagus', is a halophyte found in the estuarine and mangrove habitats of the East and West coasts of India.
What methods were used for extracting Salicornia brachiata?
The extracts were obtained using hexane, chloroform, methanol, and water through Soxhlet extraction for five to six hours under controlled temperatures.
What microorganisms were tested for antimicrobial activity?
The study tested Bacillus subtilis, Escherichia coli, Lactobacillus acidophilus, Candida albicans, and several other fungal and bacterial strains.
Which extract showed the highest antimicrobial activity?
The water extracts of Salicornia brachiata exhibited the highest antimicrobial activity against multiple tested pathogens.
How were the antimicrobial activities measured in the study?
Antimicrobial activities were measured using the Well Diffusion Method, with inhibition zones recorded in millimeters.
What is the potential use of Salicornia brachiata's extracts?
The extracts of Salicornia brachiata show potential as antimicrobial agents for treating infections caused by resistant pathogens.
Glossary definitions and references:
Scientific and Ayurvedic Glossary list for “Antimicrobial activity of Salicornia brachiata against pathogens”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.
1) Activity:
In the context of this study, 'Activity' refers to the antimicrobial effects demonstrated by the extracts of Salicornia brachiata against various pathogens. The investigation aimed to measure the degree of inhibition exhibited by plant extracts, which can be crucial in developing natural antimicrobial products and understanding plant-based medicinal properties.
2) Water:
Water in this study refers to one of the solvent extracts used to evaluate the antimicrobial properties of Salicornia brachiata. The water extract showed the highest bactericidal and fungicidal activity compared to other solvents, indicating the potential of hydrophilic compounds in the plant for therapeutic applications.
3) Table:
The term 'Table' is significant as it highlights the organization of data regarding the antimicrobial activity of different extracts of Salicornia brachiata. In scientific research, tables are essential for presenting findings clearly, such as zones of inhibition, making comparisons easy and enhancing the comprehensibility of experimental results.
4) Antibiotic (Antibacterial):
Antibiotic refers to substances that combat bacteria, a key focus of the research. The study highlights the potential of plant extracts as alternatives to traditional antibiotics, especially in light of increasing antibiotic resistance, reinforcing the value of natural sources in medicinal chemistry.
5) Andhra (Āndhra, Amdhra):
Andhra refers to Andhra Pradesh, the Indian state where the Salicornia brachiata plants were collected for this study. This geographical context is important, as it highlights the ecological diversity and specific habitats that contribute to the biological and chemical properties of the studied plant species.
6) Narasimha (Narasiṃha, Nārasiṃha, Narasingha, Nara-simha):
Narasimha pertains to G. M. Narasimha Rao, one of the authors of the study. His expertise and contributions to the research provide credibility to the results presented, emphasizing the collaborative nature of scientific exploration and the importance of knowledgeable researchers in investigating plant-based medicinal properties.
7) Botany:
Botany is the scientific study of plants, which is the field under which this research falls. This discipline is crucial in understanding plant biology, physiology, and biochemistry, particularly in investigating plant species like Salicornia brachiata for their potential use in medicine and ecology.
8) Study (Studying):
Study denotes the specific investigation conducted to evaluate the antimicrobial activity of Salicornia brachiata. Academic studies are foundational for generating knowledge, contributing to the understanding of plant-based remedies, and informing practices in agricultural and pharmaceutical applications, reflecting the significance of research in science.
9) Godavari (Godāvarī, Godāvari, Go-davari):
Godavari pertains to the estuary in Andhra Pradesh where the Salicornia brachiata was collected. This location is ecologically important as it is a habitat for halophytic species and contributes to the unique biochemical composition of the plants studied, impacting their antimicrobial properties.
10) Habitat:
Habitat signifies the natural environment where the Salicornia brachiata grows, specifically mangrove habitats. Understanding the habitat is essential as it influences the plant's adaptations and chemical constituents, which may be responsible for its observed antimicrobial activities.
11) Species:
Species refers to Salicornia brachiata, the subject of the study. It emphasizes botanical classification, underlining the importance of studying specific plants for their unique properties and potential applications in medicine, particularly regarding antimicrobial activity against human pathogens.
12) Aureus:
Aureus refers to Staphylococcus aureus, a common pathogenic bacterium tested in the study. Its significance lies in its role as a model organism for evaluating antimicrobial activity, especially in relation to the rising challenges of antibiotic resistance in infectious diseases.
13) Lakshmi (Lakṣmī):
Lakshmi denotes Prasanna Lakshmi K., one of the co-authors of the study. Her involvement signifies collaborative research efforts and emphasizes the contributions of multiple researchers in generating valuable findings that enhance the scientific community's understanding of natural antimicrobial sources.
14) India:
India is the geographical context for this research on Salicornia brachiata. Recognizing the country indicates the relevance of indigenous plants and traditional knowledge in exploring natural remedies, thereby fostering further research into the biodiversity and pharmaceutical potential found in Indian flora.
15) Rich (Ṛch):
Rich refers to the abundance of various compounds found in Salicornia brachiata that may exert antimicrobial effects. This term highlights the plant's potential as a source of bioactive substances that can be utilized in medicinal and nutritional applications, underlining the significance of biodiversity in drug discovery.
16) Lakshminarayana (Lakṣmīnārāyaṇa, Lakshmi-narayana):
Lakshmi-narayana indicates Lakshmi Narayana V., another contributor to the research. His collaboration is pertinent in establishing a cohesive team effort, essential for scientific research, which often requires a multidisciplinary approach to effectively explore the therapeutic potential of plants.
17) Discussion:
Discussion refers to the section of the research where results are interpreted in light of existing knowledge. It serves to contextualize findings within the broader scientific framework, facilitating understanding of implications, limitations, and future research directions, contributing to ongoing dialogues in biochemistry and pharmacology.
18) Developing:
Developing suggests the goal of enhancing pharmaceutical approaches through the utilization of plant extracts as novel antimicrobial agents. This term emphasizes the importance of finding sustainable and effective alternatives to conventional medicines, addressing public health needs in light of evolving antibiotic resistance.
19) Science (Scientific):
Science denotes the systematic study of the fundamental aspects of the natural world, which is the foundation of the research conducted on Salicornia brachiata. This relationship emphasizes the ongoing quest for knowledge through empirical investigation to improve health solutions and understanding biological systems.
20) Knowledge:
Knowledge denotes the insights gained from the research into the antimicrobial properties of Salicornia brachiata. This knowledge is essential for advancing the field of ethnobotany and toxicology, enabling the development of new, effective therapies derived from traditional medicines and plant resources.
21) Prasanna:
Prasanna indicates Prasanna Lakshmi K., one of the authors of the study. Identifying her contributions highlights the collaborative effort involved in this research and underlines the importance of diverse expertise in uncovering the potential of plant-derived antimicrobials.
22) Narayana (Nārāyaṇa, Nārāyana, Narāyana, Nara-ayana, Nara-yana):
Narayana refers to Lakshmi Narayana V., another key researcher in the study. His involvement represents the collective effort in investigating the uses of Salicornia brachiata, pointing to the collaborative nature of scientific inquiry in exploring and validating the potential applications of natural products.
23) Medicine:
Medicine refers to the field concerned with the treatment and prevention of diseases, where this study positions the antimicrobial properties of Salicornia brachiata. The potential therapeutic applications of plant extracts underscore the relevance of traditional botanical knowledge in contemporary medical practices.
24) Disease:
Disease represents the health conditions that the antimicrobial properties of Salicornia brachiata aim to combat. The focus on pathogens connects the research to global health issues and the need for effective treatments, particularly in the face of antibiotic-resistant strains.
25) Family:
Family refers to the taxonomic classification that groups plants with shared characteristics. In this study, Salicornia brachiata belongs to the Acanthaceae family, indicating the relevance of phylogenetics in botanical research, advancing our understanding of evolutionary relationships and ecological roles.
26) Filling (Filled):
Filled typically describes the process of introducing the extracts into wells during the antimicrobial assays. This crucial step ensures that results are standard and replicable, emphasizing the importance of methodological precision in scientific experimentation and its impact on data integrity.
27) Patra (Pātra):
Patra refers to a cited researcher, Patra, whose work is relevant in connecting findings of Salicornia brachiata with the broader context of natural product research. Such references indicate a dialogue within the scientific community that promotes the sharing and validation of research results.
28) Dish (Diś):
Dish signifies the petri dish used in microbial assays to culture organisms and evaluate antimicrobial effects. The use of dishes is vital for laboratory experiments, providing a controlled environment to observe the inhibitory actions of plant extracts on pathogens, thus informing future applications.
Other Science Concepts:
Discover the significance of concepts within the article: ‘Antimicrobial activity of Salicornia brachiata against pathogens’. Further sources in the context of Science might help you critically compare this page with similair documents:
Antimicrobial activity, Pharmaceutical industries, Chloroform extract, Water extract, Methanol extract, Well diffusion method, Zones of inhibition, Plant Extract, In Vitro Antimicrobial Activity, Hexane extract, Traditional folk medicine, Test microorganism.