Prodigiosin's antimicrobial effects on leukemia patient bacteria.

| Posted in: Health Sciences Science

Journal name: World Journal of Pharmaceutical Research
Original article title: Antimicrobial and anti adhesive properties of prodigiosin extracted from serratia marcescens against bacteria isolated from patients with leukemia.
The WJPR includes peer-reviewed publications such as scientific research papers, reports, review articles, company news, thesis reports and case studies in areas of Biology, Pharmaceutical industries and Chemical technology while incorporating ancient fields of knowledge such combining Ayurveda with scientific data.
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Original source:

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Author:

Dr. Taif H. Hussan and Sawsan H. Authaman


World Journal of Pharmaceutical Research:

(An ISO 9001:2015 Certified International Journal)

Full text available for: Antimicrobial and anti adhesive properties of prodigiosin extracted from serratia marcescens against bacteria isolated from patients with leukemia.

Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research

Copyright (license): WJPR: All rights reserved


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Summary of article contents:

1) Introduction

Prodigiosin is a red pigment produced by the bacterium Serratia marcescens, which has garnered attention for its antimicrobial, anti-adhesive, and antibiofilm properties. This study aims to investigate the effectiveness of Prodigiosin against bacteria isolated from leukemia patients in Iraq. The research highlights the optimal conditions for pigment production, the extraction and purification processes, and the pigment's effects on various bacterial strains associated with infections in leukemia patients. The findings suggest that Prodigiosin exhibits significant inhibitory effects on the growth and adhesion of bacterial pathogens, highlighting its potential therapeutic applications in managing infections.

2) Antimicrobial Activity of Prodigiosin

The study assessed the antimicrobial properties of Prodigiosin through various methods, demonstrating its effectiveness against both Gram-positive and Gram-negative bacteria. The agar well-diffusion and tube methods revealed significant inhibition percentages for several bacterial strains, with Staphylococcus epidermidis showing the highest sensitivity at 80.4%. Other bacteria like Acinetobacter baumannii and Escherichia coli also exhibited notable susceptibility, which indicates that Prodigiosin has promising antimicrobial potential for clinical use against pathogens involved in leukemia infections. These results align with existing literature that suggests gram-positive bacteria are generally more susceptible to Prodigiosin than gram-negative strains.

3) Anti-Adhesive and Antibiofilm Properties

Prodigiosin's ability to reduce bacterial adherence and biofilm formation was evaluated as key factors for its potential application in infection control. The study indicated that Prodigiosin significantly inhibited biofilm formation in various bacterial strains, shifting the classification from strong to weak biofilm-producing capabilities. For instance, the E. coli isolates displayed a substantial reduction in adhesion, with an inhibition percentage of 46.5%. This aspect of Prodigiosin is particularly relevant given the established role of biofilms in chronic infections and antibiotic resistance, highlighting the need for novel approaches to disrupt bacterial colonization and persistence.

4) Optimal Production Conditions for Prodigiosin

The research outlined the factors influencing Prodigiosin production, including incubation period, temperature, and pH. The optimal conditions identified included an incubation temperature of 30°C over 72 hours and a medium pH of 8, both of which maximized pigment yields. The study noted that variations in these conditions could significantly affect the quantity and quality of Prodigiosin produced, emphasizing the importance of optimizing fermentation parameters for enhanced production. Such findings are crucial for scaling up the production of Prodigiosin for therapeutic use, as they provide insights into the best practices for its biosynthesis.

5) Conclusion

The study concludes that Prodigiosin extracted from Serratia marcescens exhibits substantial antimicrobial, anti-adhesive, and antibiofilm properties against various bacteria isolated from leukemia patients. Its effectiveness suggests a viable potential as a therapeutic agent in managing infections, particularly for patients with compromised immune systems such as those with leukemia. Additionally, optimizing production conditions can enhance the yield of Prodigiosin, making it more feasible for clinical applications. Overall, the findings highlight Prodigiosin as a promising candidate for future research and therapeutic strategies in combating bacterial infections.

FAQ section (important questions/answers):

What is the main focus of the study?

The study investigates the antimicrobial and anti-adhesive properties of prodigiosin, extracted from Serratia marcescens, against bacteria linked with leukemia infections in Iraqi patients.

How was prodigiosin extracted in this research?

Prodigiosin was extracted using chloroform and methanol, followed by purification through acid precipitation and thin layer chromatography.

Which bacteria were tested for susceptibility to prodigiosin?

The study tested various Gram-positive and Gram-negative bacteria, including E. coli and A. baumanii, isolated from leukemia patients.

What were the results of prodigiosin's antimicrobial activity?

Prodigiosin exhibited varying inhibitory effects, with E. coli showing the highest sensitivity and A. baumanii the lowest.

What impact did prodigiosin have on biofilm formation?

Prodigiosin significantly reduced biofilm formation by pathogenic bacteria, demonstrating its potential as an anti-adhesive agent.

What was the optimum condition for prodigiosin production?

The optimum conditions for prodigiosin production were determined to be 30°C for 72 hours of incubation.

Glossary definitions and references:

Scientific and Ayurvedic Glossary list for “Prodigiosin's antimicrobial effects on leukemia patient bacteria.”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.

1) Table:
A table is a systematic arrangement of data, typically organized in rows and columns. In the context of scientific studies, tables are used to present results clearly, allowing for easy comparative analysis. The text references several tables that capture experimental data on bacterial isolates, prodigiosin production, and results of antimicrobial activity.

2) Blood:
Blood is a vital human fluid composed of cells, plasma, and platelets, essential for transporting oxygen and nutrients. In this study, blood samples from leukemia patients served as a source for isolating bacteria. The presence of certain bacteria in blood can indicate infections which may complicate leukemia treatment.

3) Activity:
Activity refers to the actions or behaviors of organisms, including their biological functions. In this research, the focus is on the antimicrobial and anti-adhesive activity of prodigiosin against bacteria causing infections in cancer patients. Understanding the activity enables the development of potential therapeutic agents.

4) Medium:
In microbiological research, a medium is a substance that supplies nutrients for the growth of microorganisms. The study utilized various growth media to cultivate Serratia marcescens. Optimal medium selection is crucial for maximizing prodigiosin production, which is vital for assessing its antimicrobial properties.

5) Cancer:
Cancer encompasses a group of diseases characterized by uncontrolled cell growth. The study discusses leukemia, a type of cancer affecting blood cells. The relevance lies in investigating bacterial infections in leukemia patients, as such infections can lead to serious complications and affect treatment outcomes.

6) Study (Studying):
A study represents a systematic investigation aimed at discovering specific facts or principles. In this context, the research focuses on evaluating the antimicrobial properties of prodigiosin derived from Serratia marcescens against bacteria isolated from leukemia patients, contributing to medical microbiology and potential treatments.

7) Antibiotic (Antibacterial):
Anti-bacterial refers to agents or substances that combat bacterial infections. Prodigiosin's classification as an anti-bacterial compound underlines its potential clinical applications. The study aims to explore the efficacy of prodigiosin in targeting specific bacterial strains, contributing to the spectrum of therapeutic choices against infections.

8) Purification:
Purification is the process of removing impurities or unwanted elements from a substance. In this study, purification techniques for extracting prodigiosin from S. marcescens were critical to ensure that the isolated pigment was suitable for evaluating its biological activities without interference from other substances.

9) Science (Scientific):
Science is the systematic study of the structure and behavior of the physical and natural world through observation and experimentation. This research falls under the branches of microbiology and pharmacology, and aims to explore natural compounds like prodigiosin to provide insights into potential medical applications.

10) Water:
Water is an essential solvent in biochemistry and microbiology, critical for sustaining life. In the conducted experiments, water was used in various extraction and purification processes of prodigiosin, highlighting its role in facilitating microbial growth and dissolving pigments during laboratory procedures.

11) Surface:
Surface refers to the outermost layer of an object. In microbiological contexts, it often pertains to the interface where microorganisms grow, such as the surfaces of culture plates. The adherence of bacteria to surfaces is central to biofilm formation, affecting both infection processes and treatment strategies.

12) Suffering:
Suffering is often related to experiencing pain or distress. The text discusses patients with leukemia suffering from infections, emphasizing the challenges they face due to weakened immune systems. This underscores the need for effective antibacterial agents to assist in their treatment and recovery.

13) Wall:
A wall can refer to the structural boundary of cells or biological entities. In microbial contexts, the cell wall is crucial for maintaining shape and integrity. The study relates to prodigiosin's effect on bacterial cell membranes, implicating changes that disrupt normal functions and promote death.

14) Soil:
Soil is a natural resource that supports plant life, containing organic matter, minerals, and microorganisms. While not directly the focus of this study, the reference to Serratia marcescens isolation could imply its environmental resilience, hinting at diverse habitats where bacteria like S. marcescens thrive.

15) Evolution:
Evolution refers to the change in the genetic composition of populations over time. This concept underpins the study by contextualizing the adaptability of bacteria like Serratia marcescens, which may evolve resistance to antibacterial substances, underscoring the need for ongoing research in microbial pharmacology.

16) Aureus:
Aureus, often referring to Staphylococcus aureus, is a common bacterium that can cause various infections. It is significant in this study, highlighting the spectrum of bacterial pathogens associated with leukemia and the relevance of evaluating the antimicrobial effects of prodigiosin against such organisms.

17) Kumar (Kumār):
Kumar typically represents a surname common in South Asia. In this context, it is part of references to collaborative authors who contributed to the research on microbial effects and prodigiosin. Recognizing contributing researchers is essential for academic accountability and credit in scientific literature.

18) Surrounding:
Surrounding pertains to the environment or conditions around a specific entity. In studies of bacteria and their interactions, surrounding conditions, including nutrient availability and temperature, significantly influence growth and metabolic activity, impacting the production of compounds like prodigiosin during investigations.

19) Discussion:
Discussion is a section in a scientific paper where results are interpreted and implications are drawn. In this study, the discussion encompasses analyzing the findings related to prodigiosin's antimicrobial properties, exploring the comprehensive implications for treatment strategies for infections caused by leukemia-associated bacteria.

20) Developing:
Developing implies the process of growth or evolution over time. In the context of this research, it pertains to the continuous effort in medical science to enhance treatment modalities against bacterial infections. The study contributes towards developing effective antibacterial agents derived from natural products like prodigiosin.

21) Detachment:
Detachment refers to the process of separation. In microbiology, it may relate to biofilm dynamics, where bacteria detach from biofilms. This study highlights prodigiosin's role in promoting the detachment of bacteria from surfaces, which is crucial for understanding biofilm control and infection management.

22) Narendra (Nara-indra):
Narendra is a common Indian name that could refer to an author or researcher in the referenced studies. Highlighting individual contributions in academic writing is important for reflecting collaboration and recognizing the efforts of numerous researchers in elucidating the properties of compounds like prodigiosin.

23) Bleeding:
Bleeding refers to the loss of blood, a significant clinical concern in leukemia patients due to associated thrombocytopenia. The study implicitly touches on the importance of managing bacterial infections to prevent complications such as prolonged bleeding, emphasizing the need for effective antibacterial treatments.

24) Cutting:
Cutting connotes the act of severing or separating. In scientific contexts, it could describe the deliberate action of isolating certain bacterial strains or segments for analysis. In this study, it may relate to methodologies used in sample preparation for evaluating the antimicrobial properties of prodigiosin.

25) Company:
Company typically denotes a business entity. In this scientific context, it may not have direct relevance, but it could refer to collaborations between academic institutions and commercial entities that facilitate research. Partnerships are crucial in advancing scientific developments and commercializing findings from such research.

26) Dealing:
Dealing references the act of managing or handling a situation. In the context of medical research, it emphasizes the challenges associated with treating infections in compromised patients, such as those suffering from leukemia. Effective dealing with bacterial threats is vital for patient health outcomes.

27) Species:
Species is a fundamental unit of biological classification and refers to a group of organisms capable of interbreeding. Within this study, the term is relevant as it identifies specific bacterial species, such as Serratia marcescens, to evaluate their susceptibility to prodigiosin, enhancing understanding of microbial diversity.

28) Sharman (Śarma, Sarmā, Sarma, Sharma, Śarman):
Sharma is a common surname that may represent one of the authors or contributors in the study or referenced literature. Highlighting various authors is essential in academic writing to ensure comprehensive acknowledgment of collaborative research efforts and the collective advancement of knowledge regarding prodigiosin.

29) Purity:
Purity denotes the absence of impurities or contaminants in a substance. In the context of this study, the purity of prodigiosin after extraction and purification is significant for ensuring accurate assessment of its biological activities, which are crucial for determining its therapeutic potential against bacterial infections.

30) Nature:
Nature refers to the inherent characteristics and qualities of entities, including biological organisms. This study delves into the natural qualities of prodigiosin, a pigment produced by Serratia marcescens, to elucidate its antimicrobial properties, showcasing the therapeutic potential of natural compounds in combatting infections.

31) Reason:
Reason signifies the explanation or justification for a particular phenomenon. In the scientific context, identifying the reasons behind the antimicrobial effects of prodigiosin against specific bacterial strains aids in elucidating mechanisms of action, guiding future research toward developing effective antibacterial therapies.

32) Farmer:
Farmer refers to a surname that typically represents an author or researcher. Within this study, it might relate to contributions in microbiology. Identifying researchers is vital in academia to recognize diverse input, validate findings, and enhance collaborative prospects in ongoing research efforts.

33) Campu (Campū, Cāmpu):
Though 'Campu' appears to be a typographical error within the text, it likely refers to ‘campus,’ indicating the institutional setting in which research and study were conducted. The campus fosters a collaborative environment vital for advancing scientific inquiry and supporting educational pursuits.

34) Glass:
Glass refers to a type of material often used in laboratory settings. Laboratory glassware is crucial for conducting experiments, as it provides environments for chemical reactions and analysis, such as in the extraction and evaluation of prodigiosin, essential to the study’s methodologies.

35) Crowd:
Crowd signifies a large number of individuals gathered in one place. In this study, a metaphor may be drawn concerning the leukemic cells overwhelming normal blood cells. Such biological overcrowding can lead to adverse health effects, emphasizing the need for efficacious treatment methods against infections.

36) Death:
Death denotes the permanent cessation of biological functions. In the context of this study, it is used to highlight the effects of prodigiosin on bacterial cells, particularly the intention to induce bacterial death as a measure of its antimicrobial efficacy in combating infections in patients.

37) Fight:
Fight refers to the act of engaging in combat or struggle. In this context, it underscores the challenge of addressing bacterial infections in patients with leukemia. Developing effective antibacterial agents is part of the larger fight against infections that threaten patient health and recovery.

38) Janta (Jamta, Jaṇṭā):
Janda represents a surname belonging to a researcher or author referenced in the study. Acknowledging various contributors and their work is crucial in academic research, highlighting collaborative efforts in investigating microbial properties and responses to compounds like prodigiosin.

39) Giri:
Giri is another surname typically associated with authors in academic literature. In this context, it emphasizes the collaborative nature of scientific research, appreciating the contributions of various researchers in exploring the applications of prodigiosin derived from Serratia marcescens.

40) Cora:
Cora indicates another surname, likely belonging to an author or researcher referenced in the associated studies. This case reiterates the importance of acknowledging contributors in the scientific community, fostering collaboration and knowledge sharing to advance understanding in fields like microbiology and pharmacology.

41) Tank (Ṭaṅk, Taṅk):
A tank in laboratory settings often refers to equipment that holds substances for experiments, such as chromatography tanks used in the purification process of prodigiosin. These setups are critical for ensuring controlled experimental conditions and accurate, reproducible results during research activities.

42) Hand:
Hand generally refers to the human extremity; however, in scientific discourse, it can symbolize manual interactions in laboratory practices. The term may also symbolize the practitioner's role in handling and executing experiments, emphasizing the human element within structured scientific methodologies.

Other Science Concepts:

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Discover the significance of concepts within the article: ‘Prodigiosin's antimicrobial effects on leukemia patient bacteria.’. Further sources in the context of Science might help you critically compare this page with similair documents:

Bone marrow, Causative factor, Incubation period, Thin Layer Chromatography, Antimicrobial properties, Antimicrobial activity, PH value, Pathogenic bacteria, Optical density, Antibacterial effect, Anti-cancer properties, Gram positive bacteria, Gram negative bacteria, Inhibitory effect, Cancer patients, Gram-negative bacilli, Gram-positive and Gram-negative, Biochemical test, Biofilm formation, Cytotoxicity activity, Bacterial isolate, Absorbance measurement, Growth Medium, Serratia marcescens, Anti adhesive properties, Antibiofilm activity, Bacterial identification, Optimum condition, Microbial Communities, Blood sample, Crystal violet, Control Well, Different temperature, Causative bacteria, High production, Amino-acid supplementation, Biochemical Characteristic.

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