Antibacterial and antifungal activities of Wrightia tinctoria extracts.
Journal name: World Journal of Pharmaceutical Research
Original article title: Evaluation of antibacterial and antifungal activitiies of the leaf and bark extracts of wrightia tinctoria r.br.
The WJPR includes peer-reviewed publications such as scientific research papers, reports, review articles, company news, thesis reports and case studies in areas of Biology, Pharmaceutical industries and Chemical technology while incorporating ancient fields of knowledge such combining Ayurveda with scientific data.
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Beena Jose, Jesy E.J.
World Journal of Pharmaceutical Research:
(An ISO 9001:2015 Certified International Journal)
Full text available for: Evaluation of antibacterial and antifungal activitiies of the leaf and bark extracts of wrightia tinctoria r.br.
Source type: An International Peer Reviewed Journal for Pharmaceutical and Medical and Scientific Research
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Summary of article contents:
Introduction
This study investigates the antibacterial and antifungal activities of leaf and bark extracts from the medicinal plant Wrightia tinctoria (Pala Indigo). Conducted by researchers Beena Jose and Jesy E.J., the research assesses the effectiveness of various solvent extracts against a range of pathogenic bacteria and fungi. The plant has a history of use in traditional medicine for treating conditions such as jaundice and skin diseases, underscoring its importance in indigenous health practices. This study aims to validate these traditional uses scientifically and explore the potential of Wrightia tinctoria as a source for antimicrobial compounds.
Antibacterial Activity of Wrightia tinctoria Extracts
The research demonstrated that methanol extracts from the leaves and bark of Wrightia tinctoria exhibited significant antibacterial properties against both Gram-positive and Gram-negative bacteria. Among the various extracts, the methanol extracts showed the strongest activity, particularly against Bacillus cereus and Staphylococcus aureus, with inhibition zones comparable to the standard antibiotics tobramycin and gentamicin sulfate. The study utilized the agar well diffusion method to measure antibacterial effectiveness, finding that different solvents influenced the extraction of active compounds, with methanol yielding the best results for antimicrobial activity.
Antifungal Activity of Wrightia tinctoria Extracts
Wrightia tinctoria extracts also displayed antifungal potential, particularly against the fungi Aspergillus niger and Penicillium chrysogenum. The methanol and ethyl acetate extracts were noted for their effectiveness in inhibiting fungal growth, and the results suggest promising applications for the plant in the treatment of fungal infections. The inhibition zones recorded for the antifungal assays reflect strong potency, indicating that the phytochemical constituents of the plant may provide valuable avenues for developing new antifungal agents, particularly in light of rising antimicrobial resistance.
Phytochemical Analysis and Implications
Phytochemical analyses revealed the presence of various bioactive compounds in the extracts, including flavonoids, phenolics, saponins, and tannins, which are known for their therapeutic properties. The presence of these phytochemicals is closely linked to the antimicrobial activity observed in the study. The extraction methods, particularly the use of methanol, seem to facilitate the release of these beneficial compounds, suggesting that careful selection of solvents can enhance the extraction of bioactive substances. This reinforces the potential for Wrightia tinctoria to contribute to the field of phytotherapy and encourages further investigation into the plant's chemical components.
Conclusion
The findings from this research confirm the antibacterial and antifungal properties of Wrightia tinctoria, supporting its use in traditional medicine and providing a scientific basis for its inclusion in future pharmacological drug formulations. The pronounced antimicrobial activities of leaf and bark methanol extracts highlight this plant's potential as a source of new antimicrobial agents. As resistance to synthetic antibiotics becomes an increasing concern, exploring natural products such as those derived from Wrightia tinctoria presents a promising avenue for addressing public health challenges related to infectious diseases. Further studies on the mechanisms of action and detailed chemical profiling of the extracts are recommended to fully elucidate their therapeutic potential.
FAQ section (important questions/answers):
What plant was studied for antibacterial and antifungal activities?
The study investigated the antibacterial and antifungal activities of Wrightia tinctoria, commonly known as Pala Indigo, focusing particularly on its leaf and bark extracts.
What methods were used to extract compounds from Wrightia tinctoria?
Compounds were extracted using sequential Soxhlet extraction with solvents like petroleum ether, chloroform, ethyl acetate, and methanol, as well as coconut oil extract from fresh leaves.
Which microorganisms were targeted in this study?
The study targeted various human pathogenic bacteria, including Bacillus cereus and Staphylococcus aureus, as well as fungi like Aspergillus niger and Penicillium chrysogenum.
How effective were the plant extracts compared to standard antibiotics?
Methanol extracts of both leaf and bark exhibited antibacterial and antifungal activities comparable to standard antibiotics like tobramycin and chloramphenicol.
What phytochemicals were found in Wrightia tinctoria extracts?
The extracts contained various phytochemicals, including flavonoids, phenols, saponins, alkaloids, glycosides, and tannins, which contribute to their antimicrobial properties.
What is the significance of this research on Wrightia tinctoria?
The study reinforces the potential of Wrightia tinctoria as a source of bioactive compounds for developing new antimicrobial agents, particularly against resistant strains.
Glossary definitions and references:
Scientific and Ayurvedic Glossary list for “Antibacterial and antifungal activities of Wrightia tinctoria extracts.”. This list explains important keywords that occur in this article and links it to the glossary for a better understanding of that concept in the context of Ayurveda and other topics.
1) Activity:
Activity refers to the capacity of substances, in this case, the extracts from Wrightia tinctoria, to exert actions against microbial pathogens. The study demonstrated that these extracts show considerable antibacterial and antifungal activity, marking their potential application in medicine and contributing to the search for new antimicrobial agents.
2) Coconut (Cocoanut):
Coconut, particularly mentioned concerning its oil, is used in the preparation of leaf extracts in this research. The oil serves as a solvent for extracting bioactive compounds that may have medicinal properties, demonstrating how traditional practices can be integrated into scientific methodologies for enhancing the efficacy of plant extracts.
3) Table:
Table signifies the systematic presentation of data within the study, allowing for clear comparison of results. Various tables in the research summarize the inhibition zones formed by the leaf and bark extracts against different microorganisms, providing a structured way to interpret the experimental findings and support conclusions drawn.
4) Aureus:
Aureus, as in Staphylococcus aureus, indicates a Gram-positive bacterium that poses significant health risks, including infections and food poisoning. The study evaluates the antimicrobial potential of Wrightia tinctoria extracts against this pathogen, highlighting their relevance in addressing antibiotic resistance and the need for alternative treatments.
5) Antibiotic (Antibacterial):
Antibiotic refers to substances that inhibit bacterial growth, used in treatment to combat infections. The study compares the efficacy of Wrightia tinctoria extracts with standard antibiotics, emphasizing their potential as alternative therapeutic agents in managing bacterial infections, particularly in the context of rising antibiotic-resistant strains.
6) Phytochemical:
Phytochemical pertains to bioactive compounds derived from plants. The research highlights the presence of various phytochemicals in Wrightia tinctoria, such as flavonoids and saponins, which are central to its medicinal properties and may contribute to its antimicrobial activities, reinforcing the importance of analyzing plant materials in drug discovery.
7) Drug:
Drug signifies any substance used in the diagnosis, cure, or prevention of diseases. The study suggests the incorporation of Wrightia tinctoria extracts into drug formulations, presenting the possibility of developing new therapeutic agents sourced from traditional medicinal plants that may meet unmet medical needs.
8) Flavonoid:
Flavonoid denotes a class of polyphenolic compounds known for various biological activities, including anti-inflammatory and antioxidant effects. Their presence in the leaf extracts of Wrightia tinctoria is significant for the observed antimicrobial activity and potentially offers health benefits, making them important in developing natural therapeutics.
9) Medicine:
Medicine encompasses the science and practice of diagnosing, treating, and preventing disease. This research emphasizes the traditional use of Wrightia tinctoria in indigenous systems, linking the plant's potential pharmacological benefits to modern medicinal practices and the importance of integrating ethnobotanical knowledge in healthcare.
10) Medium:
Medium refers to the substances or environments used for cultivating microorganisms in laboratory settings. In this study, nutrient agar and potato dextrose agar serve as media for bacterial and fungal cultures, respectively, facilitating the evaluation of antimicrobial activities and ensuring viable growth conditions for testing.
11) Disease:
Disease signifies the pathological condition affecting humans, necessitating treatment. This study aims to address communicable diseases by exploring the antimicrobial properties of plant extracts, showcasing how traditional remedies can contribute to contemporary health challenges and the potential for novel therapeutic solutions.
12) Substance:
Substance refers to any material with distinct physical or chemical properties. Here, it denotes the various extracts derived from Wrightia tinctoria containing bioactive compounds. Understanding these substances is crucial for assessing their medicinal properties and their potential integration into health applications.
13) India:
India is crucial in this research context, being the native region of Wrightia tinctoria and a hub for traditional medicinal practices. The country’s rich biodiversity and extensive use of indigenous plants provide a valuable resource for discovering new medicines and combating health issues prevalent in the population.
14) Vimala (Vimalā):
Vimala, in this context, refers to Vimala College, where the research was conducted. The institution plays a vital role in fostering scientific inquiry into traditional medicine, providing a platform for exploring the pharmacological values of local medicinal plants, thereby contributing to academic and community health knowledge.
15) Kerala (Keralā):
Kerala is a state in India known for its rich biodiversity and traditional medicine practices, such as Ayurveda. The study highlights the local availability of Wrightia tinctoria in Kerala, underscoring the significance of regional plants in both cultural practices and contemporary scientific research on medicinal properties.
16) Study (Studying):
Study signifies the systematic investigation into the antibacterial and antifungal activities of Wrightia tinctoria. It represents a structured approach to examining traditional medicine and phyto-therapeutics, demonstrating the need for scientific validation of ethnobotanical knowledge and the potential for developing new antimicrobial agents.
17) Rich (Ṛch):
Rich indicates the abundance of biodiversity and medicinal resources within the plant kingdom. The study underscores the potential of rich plant sources like Wrightia tinctoria, which contains various bioactive compounds, positioning these plants as vital contributors to the discovery of new medicines and therapeutic solutions.
18) Pharmacological:
Pharmacological pertains to the study of drug action and the interaction of substances within biological systems. This research explores the pharmacological properties of Wrightia tinctoria extracts, aiming to validate their medicinal uses and highlight their significance in developing effective treatments for bacterial and fungal infections.
19) Siddha (Siddhā):
Siddha refers to an ancient Indian system of medicine that emphasizes the use of natural substances for healing. The study's references to Siddha practices underline the traditional knowledge surrounding Wrightia tinctoria, connecting historical medical practices to modern scientific approaches in evaluating the plant's therapeutic potential.
20) Botany:
Botany is the scientific study of plants, encompassing their structure, properties, and biochemical processes. The research relies on botanical knowledge to authenticate the plant species used, emphasizing the importance of botany in pharmacognosy and natural product research for drug discovery from plant sources.
21) Glass:
Glass here suggests lab equipment, specifically glassware used in experimental procedures. Its presence in the methodology implies standard laboratory practices necessary for conducting experiments on microbial cultures and preparations of extracts, ensuring precision and reliability in the study’s findings.
22) Food:
Food, in the context of potential bacterial infections, links to the role of Staphylococcus aureus and Bacillus cereus in foodborne illnesses. The study aims to identify natural antimicrobial agents that can provide safer food practices and reduce the risks associated with contamination, advancing public health measures.
23) Snake poison (Snake venom):
Snake poison refers to the toxic substances produced by snakes, necessitating effective antidotes for treatment. The research highlights the traditional uses of Wrightia tinctoria, suggesting its ethnomedical relevance in treating snake bites, thereby bridging folk medicine and contemporary pharmacological exploration for antidote development.
24) Human body:
Human body indicates the physiological system that the studied extracts aim to affect or heal. The research underscores the significance of Wrightia tinctoria's phytochemicals in interacting with human biology to protect against or treat infections, demonstrating the importance of understanding plant-human interactions in medicine.
25) New Delhi:
New Delhi, in the context of the study, represents the administrative and academic hub of India, where regulatory bodies like the University Grants Commission support research initiatives. The city symbolizes the efforts in India to foster higher education and research in traditional medicine and pharmacology.
26) Inflammation:
Inflammation refers to the body's immune response to pathogens or injury, often resulting in symptoms like redness and swelling. The study's findings on Wrightia tinctoria's potential anti-inflammatory properties indicate its broader relevance in treatment regimens, offering insights into managing inflammation alongside infection.
27) Discussion:
Discussion denotes the section where results are interpreted and contextualized within existing knowledge. In this study, it serves to analyze the significance of Wrightia tinctoria's antimicrobial properties, linking the findings with broader themes of antibiotic resistance and the revitalization of traditional medicines in contemporary healthcare.
28) Ayurveda (Āyurveda, Ayus-veda):
Ayurveda is an ancient system of Indian medicine that uses herbal formulations for healing. The research highlights the role of Wrightia tinctoria in Ayurvedic practices, reinforcing the connection between traditional knowledge and modern scientific evaluation, and emphasizing the necessity of integrating diverse medical systems for holistic health approaches.
29) Species:
Species refers to the specific type of plant under investigation, Wrightia tinctoria. Identifying species is critical for research as it allows for the understanding and documentation of unique phytochemical properties, guiding further exploration of its medicinal uses and potential benefits within the context of health sciences.
30) Surface:
Surface refers to the outer layers of the microorganisms tested. The study implies the importance of understanding microbial surface properties as they relate to extract permeability. This knowledge is crucial for developing effective antimicrobial treatments and can influence the interactions between plant extracts and pathogenic bacteria.
31) Science (Scientific):
Science represents the systematic study of the natural world through observation and experimentation. This research exemplifies scientific inquiry into traditional medicine, validating its practices with empirical evidence, and illustrates the importance of rigorous methodologies in exploring plant-based compounds for therapeutic applications.
32) Nature:
Nature signifies the natural environment from which medicinal plants are sourced. This study underscores the importance of preserving biodiversity in nature, as it holds immense potential for discovering new drugs and understanding how various plant species can contribute to healthcare and medical advancement.
33) Indian:
Indian connects the study to its cultural and geographical context, emphasizing the significance of indigenous knowledge systems in medicine. It denotes the rich heritage of traditional practices, such as Ayurveda and Siddha, and highlights the role of local plants in addressing health challenges faced by the population.
34) Indigo:
Indigo refers to the common name associated with Wrightia tinctoria, local to certain regions and linked to its dyeing properties. The name connects the plant to its traditional uses, illustrating the cultural significance of indigenous species in various applications, including medicinal and aesthetic, within local communities.
35) Filling (Filled):
Filled, in this context, refers to the traditional practice of using Wrightia tinctoria leaves for pain relief, such as in treating toothaches. This representation of folk remedies showcases the cultural significance of plants and illustrates how traditional knowledge can influence current research into their therapeutic uses.
36) Delhi:
Delhi represents an administrative center for research institutions and funding agencies that support studies like this. It serves as a geographic nod to the regulatory and academic structures within India that facilitate scientific work on traditional medicine and provide frameworks for advancing research.
37) Water:
Water is a vital solvent and medium used throughout the experimental procedures in this study. It illustrates the importance of understanding how solvents influence the extraction of bioactive compounds from plants and emphasizes the role of solubility in determining the efficacy of antimicrobial substances extracted.
38) Fever:
Fever often indicates an underlying infection and is a common clinical symptom necessitating treatment. The study's focus on antimicrobial agents from Wrightia tinctoria relates to the need for effective treatments that can mitigate symptoms like fever, supporting the development of plant-derived medicines aimed at common health issues.
39) Dish (Diś):
Dish refers to the laboratory plates used for culturing microorganisms during the antimicrobial assays. The use of sterile dishes is critical in maintaining experimental integrity, preventing contamination, and ensuring accurate measurements of the antimicrobial activity of various extracts against the tested pathogens.
40) Sah:
Shah refers to a historical figure mentioned in the context of traditional medicine, symbolizing the continuity of using indigenous resources for health practices in India. This highlights the longstanding recognition of certain plants like Wrightia tinctoria in both historical literature and contemporary medical studies.
41) Pala (Palā, Pāla):
Pala is a common name for Wrightia tinctoria, reflecting its cultural identity and traditional uses in local healthcare practices. The name signifies the plant's importance within the community and reinforces the relevance of ethnobotanical studies in discovering effective natural remedies derived from local flora.
42) Pain (Paiṇ):
Pain signifies a symptom that commonly leads individuals to seek treatment. The relevance of Wrightia tinctoria in alleviating various types of pain showcases the plant's potential therapeutic applications, linking traditional remedies to modern medical inquiries into sourcing effective treatments for pain management.
43) Post:
Post refers to complications ensuing after medical procedures, such as infections. The study’s focus on pathogenic bacteria like Staphylococcus aureus illustrates the need for effective antimicrobial agents to prevent postoperative infections, emphasizing the relevance of exploring plant extracts as potential solutions in postoperative care.
Other Science Concepts:
Discover the significance of concepts within the article: ‘Antibacterial and antifungal activities of Wrightia tinctoria extracts.’. Further sources in the context of Science might help you critically compare this page with similair documents:
Wrightia tinctoria, Medicinal plant, Galactagogue, Traditional medicine, Synthetic antibiotic, University Grants Commission, Antibacterial activity, Bioactive substances, Phytochemical analysis, Anti-inflammatory activities, Antimicrobial activity, Zone of inhibition, Minimum inhibitory concentration, Pathogenic bacteria, Antimicrobial agent, Antifungal activity, Ethnomedicine, Staphylococcus aureus, Phytochemical evaluation, Escherichia coli, Drug formulation, Standard drug, Gram positive bacteria, Gram negative bacteria, Saponins and tannins, Agar well diffusion method, Minimum inhibitory concentration (MIC), Aspergillus niger, Anti-diabetic activity, Methanol extract, Anti pyretic, Gram positive, Gram negative, Susceptibility of microorganisms, Plant Extract, Leaf extract, Antimicrobial potency, Crude extract, Antibacterial evaluation, Standard antibiotic, Ethnomedicinal use, Bacillus cereus, Bark extract, Flavonoids phenolic compounds, Ethnobotanical importance, Phytocompound, Phytochemical compound, Multiple resistance, Active extract.